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Q03EM9 (DDL_PEDPA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:PEPE_1302
OrganismPediococcus pentosaceus (strain ATCC 25745 / 183-1w) [Complete proteome] [HAMAP]
Taxonomic identifier278197 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaePediococcus

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030476

Regions

Domain146 – 340195ATP-grasp
Nucleotide binding173 – 22654ATP By similarity

Sites

Metal binding2951Magnesium or manganese 1 By similarity
Metal binding3071Magnesium or manganese 1 By similarity
Metal binding3071Magnesium or manganese 2 By similarity
Metal binding3091Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03EM9 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: DB5C222F9E3FFE6C

FASTA35138,351
        10         20         30         40         50         60 
MKIAVLAGGK STERNVSLSS GSKITNALRS KGYDATMIDL FLGYELEDGQ SYEDVFKSSN 

        70         80         90        100        110        120 
TSTDYEISDA VLTEEDIEEL RTDGTVGLFG KNVLPILQAA DFVFLALHGG DGENGKVQAV 

       130        140        150        160        170        180 
LDLNHIKYTG SGSLASGIAM DKAISKEIML YNNIKTAQFA VLHAKDGIHP QLAFDYPMVV 

       190        200        210        220        230        240 
KPNSGGSSIG TRIVHDEAEL AESLKDAYRF DDEIIVEEFI TGREFSLGVV NGQAMPAIEI 

       250        260        270        280        290        300 
VVNDGWYDYE HKFQTGSTTK FVTPPEIEDD VHDEMKRVAV QTMDALGMTN YGRVDFLTND 

       310        320        330        340        350 
TGVYVIEANN LPGMTPLSLL PQEAEAAGIS YEDLCESIVL GKQKLYDELK K 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000422 Genomic DNA. Translation: ABJ68343.1.
RefSeqYP_804785.1. NC_008525.1.

3D structure databases

ProteinModelPortalQ03EM9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING278197.PEPE_1302.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ68343; ABJ68343; PEPE_1302.
GeneID4417557.
KEGGppe:PEPE_1302.
PATRIC22875922. VBIPedPen34213_1231.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
OMADRIQAND.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycPPEN278197:GI4Y-1302-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_PEDPA
AccessionPrimary (citable) accession number: Q03EM9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways