Q03E72 (ASNA_PEDPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	PEPE_1469

Organism

Pediococcus pentosaceus (strain ATCC 25745 / 183-1w) [Complete proteome] [HAMAP]

Taxonomic identifier

278197 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Pediococcus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017956

Sequences

	Sequence		Length	Mass (Da)
	Q03E72 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 3147D739EA3BFEC7	FASTA	335	38,834
	10 20 30 40 50 60 MDLIIPASYD PKLSVRQTQE AIRYIRETFQ DEFGKELNLS RLSAPMFVPS ATGLNDNLNG 70 80 90 100 110 120 VETPVSFSMQ DMPETSIEIV HSLAKWKRVA LKRFDFEMHS GLYTNMNAIR KDEDLDNIHS 130 140 150 160 170 180 IYVDQWDWEK VIDQSERNLT TLKETVQTIF KVIKHMEHEV WYKYPEAVYH LPDQIHFVTT 190 200 210 220 230 240 QELEDRFPKL TPKEREDAIC KELGCVFLMQ IGGTLKSGER HDGRAPDYDD WQLNGDILFW 250 260 270 280 290 300 YEPLQKALEI SSMGIRVSEE SMLKQLKIAN AEDRIHLPYH QMLLNHQLPY TIGGGIGQSR 310 320 330 LCMLLLGKAH VGEVQASVWP QSMIDQCEEN QIHIL

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25745 / 183-1w.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000422 Genomic DNA. Translation: ABJ68500.1.
RefSeq	YP_804942.1. NC_008525.1.
3D structure databases
ProteinModelPortal	Q03E72.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03E72.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4417320.
GenomeReviews	Gene locus PEPE_1469 in contig CP000422_GR.
KEGG	ppe:PEPE_1469.
NMPDR	fig|278197.10.peg.1293.
PATRIC	22876248. VBIPedPen34213_1386.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HBG288146.
OMA	LNDNLNG.
PhylomeDB	Q03E72.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	PPEN278197:PEPE_1469-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_PEDPA

Accession

Primary (citable) accession number: Q03E72

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program