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Q03C44

- PAGL1_LACC3

UniProt

Q03C44 - PAGL1_LACC3

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Protein

6-phospho-alpha-glucosidase 1

Gene

simA

Organism
Lactobacillus casei (strain ATCC 334)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Is probably involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including the five isomeric derivatives of sucrose, i.e. trehalulose-6'-phosphate, turanose-6'-phosphate, maltulose-6'-phosphate, leucrose-6'-phosphate, and palatinose-6'-phosphate, but is not active on sucrose-6-phosphate. Can also hydrolyze maltose-6'-phosphate and methyl-alpha-glucose-6-phosphate, and poorly, trehalose-6-phosphate. Fails to hydrolyze beta-O-linked phosphorylated disaccharides such as cellobiose-6'-phosphate and gentiobiose-6'-phosphate. Does not seem to be involved in maltose catabolism.1 Publication

Catalytic activityi

Alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mn2+1 Publication, Co2+1 Publication, Ni2+1 Publication, Fe2+1 Publication, Mg2+1 PublicationNote: Binds 1 divalent metal ion per subunit. Mn(2+) is the most efficient metal, but can also use Co(2+), Ni(2+) and Fe(2+), and to a lesser extent Mg(2+). Can not use Zn(2+).1 Publication
  • NAD(+)1 PublicationNote: Binds 1 NAD(+) per subunit.1 Publication

Enzyme regulationi

Is inhibited by EDTA in vitro.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951SubstrateBy similarity
Sitei111 – 1111Increases basicity of active site TyrBy similarity
Binding sitei149 – 1491SubstrateBy similarity
Metal bindingi171 – 1711ManganeseBy similarity
Active sitei172 – 1721Proton donorCurated
Metal bindingi202 – 2021ManganeseBy similarity
Active sitei265 – 2651Proton acceptorCurated
Binding sitei285 – 2851SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 7267NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. maltose-6'-phosphate glucosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Cobalt, Iron, Manganese, Metal-binding, NAD, Nickel

Enzyme and pathway databases

BioCyciLCAS321967:GH4S-368-MONOMER.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-alpha-glucosidase 1 (EC:3.2.1.122)
Gene namesi
Name:simA
Ordered Locus Names:LSEI_0369
OrganismiLactobacillus casei (strain ATCC 334)
Taxonomic identifieri321967 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001651: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4424426-phospho-alpha-glucosidase 1PRO_0000389204Add
BLAST

Expressioni

Inductioni

By maltulose, leucrose and palatinose, but not by maltose, glucose or sucrose.1 Publication

Interactioni

Subunit structurei

Homodimer. May also form homotetramer.1 Publication

Protein-protein interaction databases

STRINGi321967.LSEI_0369.

Structurei

3D structure databases

ProteinModelPortaliQ03C44.
SMRiQ03C44. Positions 4-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 4 family.Curated

Phylogenomic databases

eggNOGiCOG1486.
HOGENOMiHOG000239810.
KOiK01232.
OMAiVLAHIRV.
OrthoDBiEOG632D2X.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03C44-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDRKFSVLI AGGGSTYTPG IVLTLLDHIQ KFPLRKLKFY DIDGERQQRV
60 70 80 90 100
ADACEILVKE RAPEVEFLAT TDPEEAFTDV DFVMAQIRVG KYAMRSLDEK
110 120 130 140 150
IPLKHGVVGQ ETTGPGGIAY GLRSIPGVIG LVDYMEKYSP NAWMLNYSNP
160 170 180 190 200
AAIVAEATRR LRPHSRIINI CDMPIGIMDR MAQIVGLKDR NDLVFRYYGL
210 220 230 240 250
NHFGWWTDVR DKTGKDLMPA LKQYVAKNGY WLGDKDKDTE ASWVSTFKKA
260 270 280 290 300
ADVYALDPST LPNTYLKYYL YPKYVVEHSD PNYTRTDEVE AYREKHVFDE
310 320 330 340 350
CDRIIAAGTA ADTHFKSDDH ATYIVDLCTA IAYDTKQRML AIVPNDGAIE
360 370 380 390 400
NIDPEAMVEV PCLFGANGAE RLAMGKAATF QKGLITEQNC VEKLTVDAFE
410 420 430 440
QQSYTKLWEA MSLCKIVPDA SVAKEILDEM VVANKDYWPE LK
Length:442
Mass (Da):49,842
Last modified:November 14, 2006 - v1
Checksum:iAE4D60737BACD491
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000423 Genomic DNA. Translation: ABJ69228.1.
RefSeqiYP_805670.1. NC_008526.1.

Genome annotation databases

EnsemblBacteriaiABJ69228; ABJ69228; LSEI_0369.
GeneIDi4421568.
KEGGilca:LSEI_0369.
PATRICi22204458. VBILacCas62221_0390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000423 Genomic DNA. Translation: ABJ69228.1 .
RefSeqi YP_805670.1. NC_008526.1.

3D structure databases

ProteinModelPortali Q03C44.
SMRi Q03C44. Positions 4-441.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 321967.LSEI_0369.

Protein family/group databases

CAZyi GH4. Glycoside Hydrolase Family 4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ69228 ; ABJ69228 ; LSEI_0369 .
GeneIDi 4421568.
KEGGi lca:LSEI_0369.
PATRICi 22204458. VBILacCas62221_0390.

Phylogenomic databases

eggNOGi COG1486.
HOGENOMi HOG000239810.
KOi K01232.
OMAi VLAHIRV.
OrthoDBi EOG632D2X.

Enzyme and pathway databases

BioCyci LCAS321967:GH4S-368-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view ]
PRINTSi PR00732. GLHYDRLASE4.
SUPFAMi SSF56327. SSF56327. 1 hit.
PROSITEi PS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 334.
  2. "The sim operon facilitates the transport and metabolism of sucrose isomers in Lactobacillus casei ATCC 334."
    Thompson J., Jakubovics N., Abraham B., Hess S., Pikis A.
    J. Bacteriol. 190:3362-3373(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-30, IDENTIFICATION BY MASS SPECTROMETRY, CATALYTIC ACTIVITY, FUNCTION, SUBUNIT, ENZYME REGULATION, SUBSTRATE SPECIFICITY, COFACTOR, CATALYTIC MECHANISM, INDUCTION.

Entry informationi

Entry nameiPAGL1_LACC3
AccessioniPrimary (citable) accession number: Q03C44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 14, 2006
Last modified: November 26, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3