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Q03C44

- PAGL1_LACC3

UniProt

Q03C44 - PAGL1_LACC3

Protein

6-phospho-alpha-glucosidase 1

Gene

simA

Organism
Lactobacillus casei (strain ATCC 334)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (14 Nov 2006)
      Previous versions | rss
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    Functioni

    Is probably involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including the five isomeric derivatives of sucrose, i.e. trehalulose-6'-phosphate, turanose-6'-phosphate, maltulose-6'-phosphate, leucrose-6'-phosphate, and palatinose-6'-phosphate, but is not active on sucrose-6-phosphate. Can also hydrolyze maltose-6'-phosphate and methyl-alpha-glucose-6-phosphate, and poorly, trehalose-6-phosphate. Fails to hydrolyze beta-O-linked phosphorylated disaccharides such as cellobiose-6'-phosphate and gentiobiose-6'-phosphate. Does not seem to be involved in maltose catabolism.1 Publication

    Catalytic activityi

    Alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate.1 Publication

    Cofactori

    Binds 1 divalent metal ion per subunit. Mn2+ is the most efficient metal, but can also use Co2+, Ni2+ and Fe2+, and to a lesser extent Mg2+. Can not use Zn2+.1 Publication
    Binds 1 NAD+ per subunit.1 Publication

    Enzyme regulationi

    Is inhibited by EDTA in vitro.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei95 – 951SubstrateBy similarity
    Sitei111 – 1111Increases basicity of active site TyrBy similarity
    Binding sitei149 – 1491SubstrateBy similarity
    Metal bindingi171 – 1711ManganeseBy similarity
    Active sitei172 – 1721Proton donorCurated
    Metal bindingi202 – 2021ManganeseBy similarity
    Active sitei265 – 2651Proton acceptorCurated
    Binding sitei285 – 2851SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi6 – 7267NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. maltose-6'-phosphate glucosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Cobalt, Iron, Manganese, Metal-binding, NAD, Nickel

    Enzyme and pathway databases

    BioCyciLCAS321967:GH4S-368-MONOMER.

    Protein family/group databases

    CAZyiGH4. Glycoside Hydrolase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phospho-alpha-glucosidase 1 (EC:3.2.1.122)
    Gene namesi
    Name:simA
    Ordered Locus Names:LSEI_0369
    OrganismiLactobacillus casei (strain ATCC 334)
    Taxonomic identifieri321967 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000001651: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4424426-phospho-alpha-glucosidase 1PRO_0000389204Add
    BLAST

    Expressioni

    Inductioni

    By maltulose, leucrose and palatinose, but not by maltose, glucose or sucrose.1 Publication

    Interactioni

    Subunit structurei

    Homodimer. May also form homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi321967.LSEI_0369.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03C44.
    SMRiQ03C44. Positions 4-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 4 family.Curated

    Phylogenomic databases

    eggNOGiCOG1486.
    HOGENOMiHOG000239810.
    KOiK01232.
    OMAiVLAHIRV.
    OrthoDBiEOG632D2X.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view]
    PRINTSiPR00732. GLHYDRLASE4.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03C44-1 [UniParc]FASTAAdd to Basket

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    MDDRKFSVLI AGGGSTYTPG IVLTLLDHIQ KFPLRKLKFY DIDGERQQRV    50
    ADACEILVKE RAPEVEFLAT TDPEEAFTDV DFVMAQIRVG KYAMRSLDEK 100
    IPLKHGVVGQ ETTGPGGIAY GLRSIPGVIG LVDYMEKYSP NAWMLNYSNP 150
    AAIVAEATRR LRPHSRIINI CDMPIGIMDR MAQIVGLKDR NDLVFRYYGL 200
    NHFGWWTDVR DKTGKDLMPA LKQYVAKNGY WLGDKDKDTE ASWVSTFKKA 250
    ADVYALDPST LPNTYLKYYL YPKYVVEHSD PNYTRTDEVE AYREKHVFDE 300
    CDRIIAAGTA ADTHFKSDDH ATYIVDLCTA IAYDTKQRML AIVPNDGAIE 350
    NIDPEAMVEV PCLFGANGAE RLAMGKAATF QKGLITEQNC VEKLTVDAFE 400
    QQSYTKLWEA MSLCKIVPDA SVAKEILDEM VVANKDYWPE LK 442
    Length:442
    Mass (Da):49,842
    Last modified:November 14, 2006 - v1
    Checksum:iAE4D60737BACD491
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000423 Genomic DNA. Translation: ABJ69228.1.
    RefSeqiYP_805670.1. NC_008526.1.

    Genome annotation databases

    EnsemblBacteriaiABJ69228; ABJ69228; LSEI_0369.
    GeneIDi4421568.
    KEGGilca:LSEI_0369.
    PATRICi22204458. VBILacCas62221_0390.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000423 Genomic DNA. Translation: ABJ69228.1 .
    RefSeqi YP_805670.1. NC_008526.1.

    3D structure databases

    ProteinModelPortali Q03C44.
    SMRi Q03C44. Positions 4-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 321967.LSEI_0369.

    Protein family/group databases

    CAZyi GH4. Glycoside Hydrolase Family 4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABJ69228 ; ABJ69228 ; LSEI_0369 .
    GeneIDi 4421568.
    KEGGi lca:LSEI_0369.
    PATRICi 22204458. VBILacCas62221_0390.

    Phylogenomic databases

    eggNOGi COG1486.
    HOGENOMi HOG000239810.
    KOi K01232.
    OMAi VLAHIRV.
    OrthoDBi EOG632D2X.

    Enzyme and pathway databases

    BioCyci LCAS321967:GH4S-368-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view ]
    PRINTSi PR00732. GLHYDRLASE4.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    PROSITEi PS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 334.
    2. "The sim operon facilitates the transport and metabolism of sucrose isomers in Lactobacillus casei ATCC 334."
      Thompson J., Jakubovics N., Abraham B., Hess S., Pikis A.
      J. Bacteriol. 190:3362-3373(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-30, IDENTIFICATION BY MASS SPECTROMETRY, CATALYTIC ACTIVITY, FUNCTION, SUBUNIT, ENZYME REGULATION, SUBSTRATE SPECIFICITY, COFACTOR, CATALYTIC MECHANISM, INDUCTION.

    Entry informationi

    Entry nameiPAGL1_LACC3
    AccessioniPrimary (citable) accession number: Q03C44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: November 14, 2006
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3