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Protein

6-phospho-alpha-glucosidase 1

Gene

simA

Organism
Lactobacillus casei (strain ATCC 334)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is probably involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including the five isomeric derivatives of sucrose, i.e. trehalulose-6'-phosphate, turanose-6'-phosphate, maltulose-6'-phosphate, leucrose-6'-phosphate, and palatinose-6'-phosphate, but is not active on sucrose-6-phosphate. Can also hydrolyze maltose-6'-phosphate and methyl-alpha-glucose-6-phosphate, and poorly, trehalose-6-phosphate. Fails to hydrolyze beta-O-linked phosphorylated disaccharides such as cellobiose-6'-phosphate and gentiobiose-6'-phosphate. Does not seem to be involved in maltose catabolism.1 Publication

Catalytic activityi

Alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mn2+1 Publication, Co2+1 Publication, Ni2+1 Publication, Fe2+1 Publication, Mg2+1 PublicationNote: Binds 1 divalent metal ion per subunit. Mn2+ is the most efficient metal, but can also use Co2+, Ni2+ and Fe2+, and to a lesser extent Mg2+. Cannot use Zn2+.1 Publication
  • NAD+1 PublicationNote: Binds 1 NAD+ per subunit.1 Publication

Enzyme regulationi

Is inhibited by EDTA in vitro.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95SubstrateBy similarity1
Sitei111Increases basicity of active site TyrBy similarity1
Binding sitei149SubstrateBy similarity1
Metal bindingi171ManganeseBy similarity1
Active sitei172Proton donorCurated1
Metal bindingi202ManganeseBy similarity1
Active sitei265Proton acceptorCurated1
Binding sitei285SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 72NADBy similarityAdd BLAST67

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Cobalt, Iron, Manganese, Metal-binding, NAD, Nickel

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-alpha-glucosidase 1 (EC:3.2.1.122)
Gene namesi
Name:simA
Ordered Locus Names:LSEI_0369
OrganismiLactobacillus casei (strain ATCC 334)
Taxonomic identifieri321967 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000001651 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003892041 – 4426-phospho-alpha-glucosidase 1Add BLAST442

Expressioni

Inductioni

By maltulose, leucrose and palatinose, but not by maltose, glucose or sucrose.1 Publication

Interactioni

Subunit structurei

Homodimer. May also form homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ03C44.
SMRiQ03C44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 4 family.Curated

Phylogenomic databases

HOGENOMiHOG000239810.
KOiK01232.
OMAiKYPMREK.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03C44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDRKFSVLI AGGGSTYTPG IVLTLLDHIQ KFPLRKLKFY DIDGERQQRV
60 70 80 90 100
ADACEILVKE RAPEVEFLAT TDPEEAFTDV DFVMAQIRVG KYAMRSLDEK
110 120 130 140 150
IPLKHGVVGQ ETTGPGGIAY GLRSIPGVIG LVDYMEKYSP NAWMLNYSNP
160 170 180 190 200
AAIVAEATRR LRPHSRIINI CDMPIGIMDR MAQIVGLKDR NDLVFRYYGL
210 220 230 240 250
NHFGWWTDVR DKTGKDLMPA LKQYVAKNGY WLGDKDKDTE ASWVSTFKKA
260 270 280 290 300
ADVYALDPST LPNTYLKYYL YPKYVVEHSD PNYTRTDEVE AYREKHVFDE
310 320 330 340 350
CDRIIAAGTA ADTHFKSDDH ATYIVDLCTA IAYDTKQRML AIVPNDGAIE
360 370 380 390 400
NIDPEAMVEV PCLFGANGAE RLAMGKAATF QKGLITEQNC VEKLTVDAFE
410 420 430 440
QQSYTKLWEA MSLCKIVPDA SVAKEILDEM VVANKDYWPE LK
Length:442
Mass (Da):49,842
Last modified:November 14, 2006 - v1
Checksum:iAE4D60737BACD491
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000423 Genomic DNA. Translation: ABJ69228.1.
RefSeqiWP_011674086.1. NC_008526.1.
YP_805670.1. NC_008526.1.

Genome annotation databases

EnsemblBacteriaiABJ69228; ABJ69228; LSEI_0369.
GeneIDi4421568.
KEGGilca:LSEI_0369.
PATRICi22204458. VBILacCas62221_0390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000423 Genomic DNA. Translation: ABJ69228.1.
RefSeqiWP_011674086.1. NC_008526.1.
YP_805670.1. NC_008526.1.

3D structure databases

ProteinModelPortaliQ03C44.
SMRiQ03C44.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ69228; ABJ69228; LSEI_0369.
GeneIDi4421568.
KEGGilca:LSEI_0369.
PATRICi22204458. VBILacCas62221_0390.

Phylogenomic databases

HOGENOMiHOG000239810.
KOiK01232.
OMAiKYPMREK.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAGL1_LACC3
AccessioniPrimary (citable) accession number: Q03C44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 14, 2006
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.