Reviewed, UniProtKB/Swiss-Prot Q03AR1 (GUAC_LACC3)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	LSEI_0911

Organism

Lactobacillus casei (strain ATCC 334) [Complete proteome] [HAMAP]

Taxonomic identifier

321967 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	GMP reductase HAMAP MF_01511		PRO_0000292051
Regions
	Nucleotide binding	207 – 230	24	NADP Potential
Sites
	Active site	178	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03AR1-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 952A6568ED4643C8	FASTA	329	36,504
	10 20 30 40 50 60 MNNGMQIFDY EDIQMIPNKC VVQSRKEVDT SVKFGPHTFK IPVVPANMQT IIDEPLAIWL 70 80 90 100 110 120 AEHDYFYIMH RFQPERRMDF VRDMKKRGLI ASISVGVKDD EFDFIEALAA NELTPDYITI 130 140 150 160 170 180 DIAHGYAQVV IDMIQHIKHY LPNAFVIAGN VGTPEAVREL ENAGADATKV GIGPGKVCIT 190 200 210 220 230 240 KLKTGFGTGG WQLAAVRWCA KAARKPIIAD GGIRNNGDIA KSIRFGATMC MIGSLFAGHE 250 260 270 280 290 300 ETPGKHVNID GKEYQEYYGS ASEYQKGTHH NVEGKKILLP VKGKIGDTLK EMQEDLQSAV 310 320 SYAGGRDLES LTKVDYVIVK NSIFNGDQY

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000423 Genomic DNA. Translation: ABJ69711.1.
RefSeq	YP_806153.1.
3D structure databases
SMR	Q03AR1. Positions 7-324.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03AR1.
Genome annotation databases
GeneID	4419308.
GenomeReviews	Gene locus LSEI_0911 in contig CP000423_GR.
KEGG	lca:LSEI_0911.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0516.
HOGENOM	HBG298985.
OMA	FAGHEES.
PhylomeDB	Q03AR1.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_LACC3

Accession

Primary (citable) accession number: Q03AR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	November 14, 2006
Last modified:	February 9, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)