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Q039Q9

- SYI_LACC3

UniProt

Q039Q9 - SYI_LACC3

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus casei (strain ATCC 334)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi887 – 8871ZincUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLCAS321967:GH4S-1279-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LSEI_1280
OrganismiLactobacillus casei (strain ATCC 334)
Taxonomic identifieri321967 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001651: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 928928Isoleucine--tRNA ligasePRO_1000022082Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi321967.LSEI_1280.

Structurei

3D structure databases

ProteinModelPortaliQ039Q9.
SMRiQ039Q9. Positions 1-916.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q039Q9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIKDTLNMG KTSFPMRAGL PKNESIWQKK WDEDHVYEQR QKLNEGKPTF
60 70 80 90 100
MLHDGPPFAN GNIHMGHALN KISKDIIVRY KSMNGFRAPY VPGWDTHGLP
110 120 130 140 150
IEQQLAKQGV KRKEMSMTDY RELCRQFAMQ EIDKQRTDFK RLGVMGDWEH
160 170 180 190 200
PYITLQHHYE ASEIRVFGAM AKKGYIYHGL KPVYWSWSSE STLAEAEIEY
210 220 230 240 250
HDDKSPSIYV AFKVVDGKGL LDTDTYLVIW TTTPWTIPAN YGITVNPRFD
260 270 280 290 300
YVQVQVGDKK YVVAAELLDR VAEEIGWENP KILKTFKGTD MDKMTAQHPL
310 320 330 340 350
YDRTSLVMNA DHVTLDAGTG LVHTAPGHGE DDYKVGVKYG LPVVSVVDAK
360 370 380 390 400
GYMNEYAPGF EGVFYDDANK QITQALADKG ALLKLDFFTH SYPHDWRTKK
410 420 430 440 450
PVIFRATTQW FASIDAFRDQ ILKAIDTVDF KPSWGKTRLY NMIRDRGDWV
460 470 480 490 500
ISRQRAWGVP LPIFYAEDGE PIIEEETINH VADLFGKYGS NVWFEREAKD
510 520 530 540 550
LLPEGYTNPH SPNGQFTKEK DIMDVWFDSG SSHQAVLAAR PELSFPADLY
560 570 580 590 600
LEGSDQYRGW FNSSLITSVA ATGVAPYRGI LSQGFTLDGK GRKMSKSLGN
610 620 630 640 650
TIVPATIEKQ FGAEIIRLWV ATVDSSSDVR VSVDNFAQTS EAYRKIRNTM
660 670 680 690 700
RFMVANTGDF DPEKDTVAYD ELGSVDRYMM VRLNQIIKQV KTAYDAYDFA
710 720 730 740 750
TVEKTISSFL VNDLSAFYLD VAKDVVYIEA KDDPKRRGMQ TVMFAALLAL
760 770 780 790 800
TKLITPILPH TAEEVWPYLK QPEAYAALAD MPEAEQFDDE SQLLDIWSGF
810 820 830 840 850
MDFRSEVQKA LELARDNKVI GKSLEAAVTV YPSEPVRDML DDVDANVMQL
860 870 880 890 900
LITSHFEIAP ATTQAPANAE QFDDMAVVVK HADGEVCPRC RMVRTDIGTD
910 920
PKLPQLCSRC AAIVEANFPD AVTNGFDK
Length:928
Mass (Da):105,013
Last modified:November 14, 2006 - v1
Checksum:iD85AD91B2A0D64AA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000423 Genomic DNA. Translation: ABJ70063.1.
RefSeqiWP_011674453.1. NC_008526.1.
YP_806505.1. NC_008526.1.

Genome annotation databases

EnsemblBacteriaiABJ70063; ABJ70063; LSEI_1280.
GeneIDi4419576.
KEGGilca:LSEI_1280.
PATRICi22206280. VBILacCas62221_1258.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000423 Genomic DNA. Translation: ABJ70063.1 .
RefSeqi WP_011674453.1. NC_008526.1.
YP_806505.1. NC_008526.1.

3D structure databases

ProteinModelPortali Q039Q9.
SMRi Q039Q9. Positions 1-916.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 321967.LSEI_1280.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ70063 ; ABJ70063 ; LSEI_1280 .
GeneIDi 4419576.
KEGGi lca:LSEI_1280.
PATRICi 22206280. VBILacCas62221_1258.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LCAS321967:GH4S-1279-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 334.

Entry informationi

Entry nameiSYI_LACC3
AccessioniPrimary (citable) accession number: Q039Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3