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Q039Q9

- SYI_LACC3

UniProt

Q039Q9 - SYI_LACC3

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus casei (strain ATCC 334)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (14 Nov 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
    Binding sitei596 – 5961ATPUniRule annotation
    Metal bindingi887 – 8871ZincUniRule annotation
    Metal bindingi890 – 8901ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLCAS321967:GH4S-1279-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:LSEI_1280
    OrganismiLactobacillus casei (strain ATCC 334)
    Taxonomic identifieri321967 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000001651: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 928928Isoleucine--tRNA ligasePRO_1000022082Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi321967.LSEI_1280.

    Structurei

    3D structure databases

    ProteinModelPortaliQ039Q9.
    SMRiQ039Q9. Positions 1-916.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGRRSCQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q039Q9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIKDTLNMG KTSFPMRAGL PKNESIWQKK WDEDHVYEQR QKLNEGKPTF    50
    MLHDGPPFAN GNIHMGHALN KISKDIIVRY KSMNGFRAPY VPGWDTHGLP 100
    IEQQLAKQGV KRKEMSMTDY RELCRQFAMQ EIDKQRTDFK RLGVMGDWEH 150
    PYITLQHHYE ASEIRVFGAM AKKGYIYHGL KPVYWSWSSE STLAEAEIEY 200
    HDDKSPSIYV AFKVVDGKGL LDTDTYLVIW TTTPWTIPAN YGITVNPRFD 250
    YVQVQVGDKK YVVAAELLDR VAEEIGWENP KILKTFKGTD MDKMTAQHPL 300
    YDRTSLVMNA DHVTLDAGTG LVHTAPGHGE DDYKVGVKYG LPVVSVVDAK 350
    GYMNEYAPGF EGVFYDDANK QITQALADKG ALLKLDFFTH SYPHDWRTKK 400
    PVIFRATTQW FASIDAFRDQ ILKAIDTVDF KPSWGKTRLY NMIRDRGDWV 450
    ISRQRAWGVP LPIFYAEDGE PIIEEETINH VADLFGKYGS NVWFEREAKD 500
    LLPEGYTNPH SPNGQFTKEK DIMDVWFDSG SSHQAVLAAR PELSFPADLY 550
    LEGSDQYRGW FNSSLITSVA ATGVAPYRGI LSQGFTLDGK GRKMSKSLGN 600
    TIVPATIEKQ FGAEIIRLWV ATVDSSSDVR VSVDNFAQTS EAYRKIRNTM 650
    RFMVANTGDF DPEKDTVAYD ELGSVDRYMM VRLNQIIKQV KTAYDAYDFA 700
    TVEKTISSFL VNDLSAFYLD VAKDVVYIEA KDDPKRRGMQ TVMFAALLAL 750
    TKLITPILPH TAEEVWPYLK QPEAYAALAD MPEAEQFDDE SQLLDIWSGF 800
    MDFRSEVQKA LELARDNKVI GKSLEAAVTV YPSEPVRDML DDVDANVMQL 850
    LITSHFEIAP ATTQAPANAE QFDDMAVVVK HADGEVCPRC RMVRTDIGTD 900
    PKLPQLCSRC AAIVEANFPD AVTNGFDK 928
    Length:928
    Mass (Da):105,013
    Last modified:November 14, 2006 - v1
    Checksum:iD85AD91B2A0D64AA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000423 Genomic DNA. Translation: ABJ70063.1.
    RefSeqiYP_806505.1. NC_008526.1.

    Genome annotation databases

    EnsemblBacteriaiABJ70063; ABJ70063; LSEI_1280.
    GeneIDi4419576.
    KEGGilca:LSEI_1280.
    PATRICi22206280. VBILacCas62221_1258.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000423 Genomic DNA. Translation: ABJ70063.1 .
    RefSeqi YP_806505.1. NC_008526.1.

    3D structure databases

    ProteinModelPortali Q039Q9.
    SMRi Q039Q9. Positions 1-916.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 321967.LSEI_1280.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABJ70063 ; ABJ70063 ; LSEI_1280 .
    GeneIDi 4419576.
    KEGGi lca:LSEI_1280.
    PATRICi 22206280. VBILacCas62221_1258.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGRRSCQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LCAS321967:GH4S-1279-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 334.

    Entry informationi

    Entry nameiSYI_LACC3
    AccessioniPrimary (citable) accession number: Q039Q9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 14, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3