Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

AT-rich interactive domain-containing protein 5A

Gene

ARID5A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to AT-rich stretches in the modulator region upstream of the human cytomegalovirus major intermediate early gene enhancer. May act as repressor and down-regulate enhancer-dependent gene expressison (PubMed:8649988). May positively regulate chondrocyte-specific transcription such as of COL2A1 in collaboration with SOX9 and positively regulate histone H3 acetylation at chondrocyte-specific genes. May stimulate early-stage chondrocyte differentiation and inhibit later stage differention (By similarity). Can repress ESR1-mediated transcriptional activation; proposed to act as corepressor for selective nuclear hormone receptors (PubMed:15941852). As RNA-binding protein involved in the regulation of inflammatory response by stabilizing selective inflammation-related mRNAs, such as IL6, STAT3 and TBX21. Binds to stem loop structures located in the 3'UTRs of IL6, STAT3 and TBX21 mRNAs; at least for STAT3 prevents binding of ZC3H12A to the mRNA stem loop structure thus inhibiting its degradation activity. Contributes to elevated IL6 levels possibly implicated in autoimmunity processes. IL6-dependent stabilization of STAT3 mRNA may promote differentiation of naive CD4+ T-cells into T-helper Th17 cells. In CD4+ T-cells may also inhibit RORC-induced Th17 cell differentiation independently of IL6 signaling. Stabilization of TBX21 mRNA contributes to elevated interferon-gamma secretion in Th1 cells possibly implicated in the establishment of septic shock (By similarity).By similarity2 Publications

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • DNA binding Source: GDB
  • estrogen receptor binding Source: UniProtKB
  • retinoid X receptor binding Source: UniProtKB
  • RNA binding Source: UniProtKB-KW
  • thyroid hormone receptor binding Source: UniProtKB
  • transcription corepressor activity Source: UniProtKB
  • transcription factor binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: GO_Central

GO - Biological processi

  • cellular response to estrogen stimulus Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: GDB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding, Repressor, RNA-binding
Biological processImmunity, Innate immunity, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
AT-rich interactive domain-containing protein 5A
Short name:
ARID domain-containing protein 5A
Alternative name(s):
Modulator recognition factor 1
Short name:
MRF-1
Gene namesi
Name:ARID5A
Synonyms:MRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:17361. ARID5A.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000196843.
PharmGKBiPA134937822.

Polymorphism and mutation databases

BioMutaiARID5A.
DMDMi148840818.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002889301 – 594AT-rich interactive domain-containing protein 5AAdd BLAST594

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineBy similarity1
Cross-linki85Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki94Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei256PhosphoserineBy similarity1
Modified residuei289PhosphoserineBy similarity1
Modified residuei438PhosphoserineBy similarity1
Modified residuei463PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by MAPK14 on serine residues involving a TLR4 signaling pathway upon lipopolysaccharide (LPS) stimulation leading to its ubiquitination and proteasomal degradation.By similarity
Ubiquitinated leading to proteasomal degradation; involving WWP1 linked to MAPK14-mediated phosphorylation upon LPS stimulation.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ03989.
MaxQBiQ03989.
PaxDbiQ03989.
PeptideAtlasiQ03989.
PRIDEiQ03989.

PTM databases

iPTMnetiQ03989.
PhosphoSitePlusiQ03989.

Expressioni

Gene expression databases

BgeeiENSG00000196843.
CleanExiHS_ARID5A.
ExpressionAtlasiQ03989. baseline and differential.
GenevisibleiQ03989. HS.

Organism-specific databases

HPAiHPA002860.
HPA023879.

Interactioni

Subunit structurei

Interacts with SOX9 (By similarity). Interacts with ESR1 (PubMed:15941852). Interacts with RORC (By similarity).By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • estrogen receptor binding Source: UniProtKB
  • retinoid X receptor binding Source: UniProtKB
  • thyroid hormone receptor binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116074. 20 interactors.
IntActiQ03989. 103 interactors.
MINTiMINT-2868767.
STRINGi9606.ENSP00000350078.

Structurei

3D structure databases

ProteinModelPortaliQ03989.
SMRiQ03989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 147ARIDPROSITE-ProRule annotationAdd BLAST93

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 300Interaction with SOX9By similarityAdd BLAST300

Phylogenomic databases

eggNOGiKOG2744. Eukaryota.
ENOG410Y2AP. LUCA.
GeneTreeiENSGT00890000139441.
HOGENOMiHOG000034050.
HOVERGENiHBG062210.
InParanoidiQ03989.
OMAiHFFHLNT.
OrthoDBiEOG091G0E6N.
PhylomeDBiQ03989.
TreeFamiTF324725.

Family and domain databases

InterProiView protein in InterPro
IPR001606. ARID_dom.
PfamiView protein in Pfam
PF01388. ARID. 1 hit.
SMARTiView protein in SMART
SM00501. BRIGHT. 1 hit.
SUPFAMiSSF46774. SSF46774. 1 hit.
PROSITEiView protein in PROSITE
PS51011. ARID. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q03989-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPVKGNRK QSTEGDALDP PASPKPAGKQ NGIQNPISLE DSPEAGGERE
60 70 80 90 100
EEQEREEEQA FLVSLYKFMK ERHTPIERVP HLGFKQINLW KIYKAVEKLG
110 120 130 140 150
AYELVTGRRL WKNVYDELGG SPGSTSAATC TRRHYERLVL PYVRHLKGED
160 170 180 190 200
DKPLPTSKPR KQYKMAKENR GDDGATERPK KAKEERRMDQ MMPGKTKADA
210 220 230 240 250
ADPAPLPSQE PPRNSTEQQG LASGSSVSFV GASGCPEAYK RLLSSFYCKG
260 270 280 290 300
THGIMSPLAK KKLLAQVSKV EALQCQEEGC RHGAEPQASP AVHLPESPQS
310 320 330 340 350
PKGLTENSRH RLTPQEGLQA PGGSLREEAQ AGPCPAAPIF KGCFYTHPTE
360 370 380 390 400
VLKPVSQHPR DFFSRLKDGV LLGPPGKEGL SVKEPQLVWG GDANRPSAFH
410 420 430 440 450
KGGSRKGILY PKPKACWVSP MAKVPAESPT LPPTFPSSPG LGSKRSLEEE
460 470 480 490 500
GAAHSGKRLR AVSPFLKEAD AKKCGAKPAG SGLVSCLLGP ALGPVPPEAY
510 520 530 540 550
RGTMLHCPLN FTGTPGPLKG QAALPFSPLV IPAFPAHFLA TAGPSPMAAG
560 570 580 590
LMHFPPTSFD SALRHRLCPA SSAWHAPPVT TYAAPHFFHL NTKL
Length:594
Mass (Da):64,074
Last modified:May 29, 2007 - v2
Checksum:i89A494C4560416FF
GO
Isoform 2 (identifier: Q03989-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Show »
Length:526
Mass (Da):56,694
Checksum:i2CB14C05D57D1A35
GO

Sequence cautioni

The sequence AAA36325 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti392D → G in BC047390 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0589691 – 68Missing in isoform 2. Add BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62324 mRNA. Translation: AAA36325.1. Different initiation.
AC013270 Genomic DNA. No translation available.
CH471207 Genomic DNA. Translation: EAW71359.1.
BC047390 mRNA. No translation available.
CCDSiCCDS33251.1. [Q03989-1]
CCDS82484.1. [Q03989-5]
PIRiS27962.
RefSeqiNP_001306021.1. NM_001319092.1. [Q03989-5]
NP_997646.1. NM_212481.2. [Q03989-1]
UniGeneiHs.920.

Genome annotation databases

EnsembliENST00000357485; ENSP00000350078; ENSG00000196843. [Q03989-1]
ENST00000454558; ENSP00000400785; ENSG00000196843. [Q03989-5]
GeneIDi10865.
KEGGihsa:10865.
UCSCiuc002swe.4. human. [Q03989-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARI5A_HUMAN
AccessioniPrimary (citable) accession number: Q03989
Secondary accession number(s): C9J1Q0, Q6NX37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: August 30, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot