Reviewed, UniProtKB/Swiss-Prot Q03986 (ERMD_BACLI)

Names and origin

Protein names

Recommended name:
    rRNA adenine N-6-methyltransferase
    EC=2.1.1.48
Alternative name(s):
    Macrolide-lincosamide-streptogramin B resistance protein
    Erythromycin resistance protein

Gene names

Name:

ermD

Organism

Bacillus licheniformis

Taxonomic identifier

1402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein produces a dimethylation of the adenine residue at position 2058 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine.
Induction	By erythromycin and oleandomycin.
Sequence similarities	Belongs to the rRNA adenine N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	rRNA adenine N-6-methyltransferase		PRO_0000101669

Sequences

	Sequence		Length	Mass (Da)
	Q03986-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D3227F255A729060	FASTA	287	32,798
	10 20 30 40 50 60 MKKKNHKYRG KKLNRGESPN FSGQHLMHNK KLIEEIVDRA NISIDDTVLE LGAGKGALTT 70 80 90 100 110 120 VLSQKAGKVL AVENDSKFVD ILTRKTAQHS NTKIIHQDIM KIHLPKEKFV VVSNIPYAIT 130 140 150 160 170 180 TPIMKMLLNN PASGFQKGII VMEKGAAKRF TSKFIKNSYV LAWRMWFDIG IVREISKEHF 190 200 210 220 230 240 SPPPKVDSAM VRITRKKDAP LSHKHYIAFR GLAEYALKEP NIPLCVALRG IFTPRQMKHL 250 260 270 280 RKSLKINNEK TVGTLTENQW AVIFNTMTQY VMHHKWPRAN KRKPGEI

References

[1]	"DNA sequence and regulation of ermD, a macrolide-lincosamide-streptogramin B resistance element from Bacillus licheniformis." Gryczan T., Israeli-Reches M., del Bue M., Dubnau D. Mol. Gen. Genet. 194:349-356(1984) [PubMed: 6429477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M29832 Genomic DNA. Translation: AAA22599.1.
PIR	I39885.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.48. 1017.
Family and domain databases
InterPro	IPR001737. RRNA_Ade_Mease_Trfase. [Graphical view]
PANTHER	PTHR11727. RRNA_meth_trans. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERMD_BACLI

Accession

Primary (citable) accession number: Q03986

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	March 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)