Laccase precursor - Cryphonectria parasitica (Chesnut blight fungus)

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Reviewed, UniProtKB/Swiss-Prot Q03966 (LAC1_CRYPA)

Last modified November 25, 2008. Version 54. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Laccase
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase
    Urishiol oxidase
    Diphenol oxidase

Gene names

Name:

LAC-1

Organism

Cryphonectria parasitica (Chesnut blight fungus) (Endothia parasitica)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Diaporthales › Cryphonectriaceae › Cryphonectria-Endothia complex › Cryphonectria

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	SecretedPotential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

20

Potential

Chain

571

Laccase

PRO_0000002920

Regions

Domain

124

Plastocyanin-like 1

Domain

159

Plastocyanin-like 2

Domain

136

Plastocyanin-like 3

Sites

Metal binding

1

Copper 1; type 2 By similarity

Metal binding

1

Copper 2; type 3 By similarity

Metal binding

1

Copper 2; type 3 By similarity

Metal binding

1

Copper 3; type 3 By similarity

Metal binding

1

Copper 4; type 1 By similarity

Metal binding

1

Copper 1; type 2 By similarity

Metal binding

1

Copper 3; type 3 By similarity

Metal binding

1

Copper 3; type 3 By similarity

Metal binding

1

Copper 4; type 1 By similarity

Metal binding

1

Copper 2; type 3 By similarity

Metal binding

1

Copper 4; type 1 By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q03966-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B2F44CB2AAD77701
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591

64,696

        10         20         30         40         50         60 
MPSFFRALFS GLIASQLSWA APSLLHPLEP RQQPNCNTAS NRACWISGSY DITTDYEVKT 

        70         80         90        100        110        120 
PLTGVVRQYD LTLTQAENWL GPDGVVKEDV MLVNGNILGP VIHAQWGDTI SVTVTNNLKY 

       130        140        150        160        170        180 
NGTTIHWHGI RQLNTNLQDG VNGITECPIP PNGGSKTYTF IAHQYGTSWY HSHFSAQYGN 

       190        200        210        220        230        240 
GIVGAIQIDG PASLPYDIDL GPLVLSDYYY KTADELVVYT QSNAPPASDN VLFNGTNINP 

       250        260        270        280        290        300 
ANTTQGQYKT ITLTPGKRHR LRIINTSVEN NFQVSIVGHS MTVIESDFVP VDSFTTDSLF 

       310        320        330        340        350        360 
VGIGQRYDVT IDASQATDNY WMNVTFGGGG FCGKSNNPYP AAIIHYNGAS NSHPTNKGVA 

       370        380        390        400        410        420 
PADHECLDLL NLVPVVPRSI PTSGFVAASD NTLDVQLSTT TRKWTINGST LDVDWGHPIT 

       430        440        450        460        470        480 
QYVINKSTAW PSTDNVWLVE EANQWAYWLI ENDPTATGNA LPHPIHLHGH DFVVLGRSPN 

       490        500        510        520        530        540 
VSPTAQTPYT FTSSDVSSLN GNNPIRRDVV MLPPKGWLLI AFQTTNPGAW LMHCHIAWHV 

       550        560        570        580        590 
SAGLGNTFLE QPSAFVAGLN TNDVNQLNSQ CKSWNAYYPS KDIFKQDDSG V

References

[1]

"Molecular analysis of the laccase gene from the chestnut blight fungus and selective suppression of its expression in an isogenic hypovirulent strain."
Choi G.H., Larson T.G., Nuss D.L.
Mol. Plant Microbe Interact. 5:119-128(1992) [PubMed: 1535523] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
	M73257 Genomic DNA. Translation: AAA33105.1. S38903 Genomic DNA. Translation: AAA09235.1.
3D structure databases
HSSP	HSSP built from PDB template 1HFU based on UniProtKB Q9Y780.
ModBase	Search...
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC1_CRYPA

Accession

Primary (citable) accession number: Q03966

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents