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Q03940

- RUVB1_YEAST

UniProt

Q03940 - RUVB1_YEAST

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Protein

RuvB-like protein 1

Gene

RVB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

DNA helicase which participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes. The SWR1 complex mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. The INO80 complex remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to 'Ser-129' phosphorylated H2A histones as a response to DNA damage. RVB1 recruits ARP5 to the INO80 complex. During transcription may recruit SPT15/TBP to the TATA-boxes of involved genes. Required for box C/D and box H/ACA snoRNA accumulation and involved in pre-rRNA processing.6 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi79 – 868ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent 5'-3' DNA helicase activity Source: SGD

GO - Biological processi

  1. box C/D snoRNP assembly Source: SGD
  2. chromatin remodeling Source: SGD
  3. DNA duplex unwinding Source: GOC
  4. DNA repair Source: UniProtKB-KW
  5. histone exchange Source: SGD
  6. regulation of transcription from RNA polymerase II promoter Source: SGD
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29778-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RuvB-like protein 1 (EC:3.6.4.12)
Short name:
RUVBL1
Alternative name(s):
TIP49-homology protein 1
TIP49a homolog
Gene namesi
Name:RVB1
Synonyms:TIH1, TIP49A
Ordered Locus Names:YDR190C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR190c.
SGDiS000002598. RVB1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. Ino80 complex Source: SGD
  2. nucleus Source: SGD
  3. R2TP complex Source: SGD
  4. Swr1 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi85 – 851K → E: Lethal. 1 Publication
Mutagenesisi312 – 3121E → G: Lethal. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463RuvB-like protein 1PRO_0000165661Add
BLAST

Proteomic databases

MaxQBiQ03940.
PaxDbiQ03940.
PeptideAtlasiQ03940.

Expressioni

Gene expression databases

GenevestigatoriQ03940.

Interactioni

Subunit structurei

Probably forms a homohexamer. Interacts with RVB2 and may form heterododecamers with RVB2. Component of the SWR1 chromatin remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80. Component of the R2TP complex composed of at least RVB1, RVB2, TAH1 and PIH1. Interacts with SPT15/TBP and HSP90.9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PIH1P387686EBI-30712,EBI-24499
RVB2Q1246410EBI-30712,EBI-31814
SPT15P133933EBI-30712,EBI-19129

Protein-protein interaction databases

BioGridi32243. 156 interactions.
DIPiDIP-4896N.
IntActiQ03940. 130 interactions.
MINTiMINT-523823.
STRINGi4932.YDR190C.

Structurei

3D structure databases

ProteinModelPortaliQ03940.
SMRiQ03940. Positions 23-456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.Curated

Phylogenomic databases

eggNOGiCOG1224.
GeneTreeiENSGT00550000075043.
HOGENOMiHOG000190885.
InParanoidiQ03940.
KOiK04499.
OMAiNKVVSKY.
OrthoDBiEOG7FBRT0.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR027238. RuvB-like.
IPR010339. TIP49_C.
[Graphical view]
PANTHERiPTHR11093. PTHR11093. 1 hit.
PfamiPF06068. TIP49. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q03940-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVAISEVKEN PGVNSSNSGA VTRTAAHTHI KGLGLDESGV AKRVEGGFVG
60 70 80 90 100
QIEAREACGV IVDLIKAKKM SGRAILLAGG PSTGKTALAL AISQELGPKV
110 120 130 140 150
PFCPLVGSEL YSVEVKKTET LMENFRRAIG LRIKETKEVY EGEVTELTPE
160 170 180 190 200
DAENPLGGYG KTISHVIVGL KSAKGTKTLR LDPTIYESIQ REKVSIGDVI
210 220 230 240 250
YIEANTGAVK RVGRSDAYAT EFDLETEEYV PLPKGEVHKK KEIVQDVTLH
260 270 280 290 300
DLDVANARPQ GGQDVISMMG QLLKPKKTEI TEKLRQEVNK VVAKYIDQGV
310 320 330 340 350
AELIPGVLFI DEVNMLDIEI FTYLNKALES NIAPVVVLAS NRGMTTVRGT
360 370 380 390 400
EDVISPHGVP PDLIDRLLIV RTLPYDKDEI RTIIERRATV ERLQVESSAL
410 420 430 440 450
DLLATMGTET SLRYALQLLA PCGILAQTSN RKEIVVNDVN EAKLLFLDAK
460
RSTKILETSA NYL
Length:463
Mass (Da):50,453
Last modified:November 1, 1996 - v1
Checksum:i93D6ECB7006AF0F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48784 Genomic DNA. Translation: CAA88704.1.
BK006938 Genomic DNA. Translation: DAA12033.1.
PIRiS52698.
RefSeqiNP_010476.1. NM_001180498.1.

Genome annotation databases

EnsemblFungiiYDR190C; YDR190C; YDR190C.
GeneIDi851771.
KEGGisce:YDR190C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48784 Genomic DNA. Translation: CAA88704.1 .
BK006938 Genomic DNA. Translation: DAA12033.1 .
PIRi S52698.
RefSeqi NP_010476.1. NM_001180498.1.

3D structure databases

ProteinModelPortali Q03940.
SMRi Q03940. Positions 23-456.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32243. 156 interactions.
DIPi DIP-4896N.
IntActi Q03940. 130 interactions.
MINTi MINT-523823.
STRINGi 4932.YDR190C.

Proteomic databases

MaxQBi Q03940.
PaxDbi Q03940.
PeptideAtlasi Q03940.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR190C ; YDR190C ; YDR190C .
GeneIDi 851771.
KEGGi sce:YDR190C.

Organism-specific databases

CYGDi YDR190c.
SGDi S000002598. RVB1.

Phylogenomic databases

eggNOGi COG1224.
GeneTreei ENSGT00550000075043.
HOGENOMi HOG000190885.
InParanoidi Q03940.
KOi K04499.
OMAi NKVVSKY.
OrthoDBi EOG7FBRT0.

Enzyme and pathway databases

BioCyci YEAST:G3O-29778-MONOMER.

Miscellaneous databases

NextBioi 969564.
PROi Q03940.

Gene expression databases

Genevestigatori Q03940.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR027238. RuvB-like.
IPR010339. TIP49_C.
[Graphical view ]
PANTHERi PTHR11093. PTHR11093. 1 hit.
Pfami PF06068. TIP49. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The Saccharomyces cerevisiae RuvB-like protein, Tih2p, is required for cell cycle progression and RNA polymerase II-directed transcription."
    Lim C.R., Kimata Y., Ohdate H., Kokubo T., Kikuchi N., Horigome T., Kohno K.
    J. Biol. Chem. 275:22409-22417(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RVB2.
  4. "A chromatin remodelling complex involved in transcription and DNA processing."
    Shen X., Mizuguchi G., Hamiche A., Wu C.
    Nature 406:541-544(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE INO80 COMPLEX, FUNCTION OF THE INO80 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes."
    Jonsson Z.O., Dhar S.K., Narlikar G.J., Auty R., Wagle N., Pellman D., Pratt R.E., Kingston R., Dutta A.
    J. Biol. Chem. 276:16279-16288(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RVB2, IDENTIFICATION IN A COMPLEX WITH RBV2; ACT1 AND ARP4, IDENTIFICATION BY MASS SPECTROMETRY, MUTAGENESIS OF LYS-85 AND GLU-312.
  6. "Impairment of the DNA binding activity of the TATA-binding protein renders the transcriptional function of Rvb2p/Tih2p, the yeast RuvB-like protein, essential for cell growth."
    Ohdate H., Lim C.R., Kokubo T., Matsubara K., Kimata Y., Kohno K.
    J. Biol. Chem. 278:14647-14656(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPT15.
  7. Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex."
    Jonsson Z.O., Jha S., Wohlschlegel J.A., Dutta A.
    Mol. Cell 16:465-477(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE INO80 COMPLEX, IDENTIFICATION BY IDENTIFICATION BY MASS SPECTROMETRY.
  11. "A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin."
    Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J.
    PLoS Biol. 2:587-599(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  12. "ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex."
    Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C.
    Science 303:343-348(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  13. "Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone."
    Zhao R., Davey M.G., Hsu Y.-C., Kaplanek P., Tong A., Parsons A.B., Krogan N.J., Cagney G., Mai D., Greenblatt J.F., Boone C., Emili A., Houry W.A.
    Cell 120:715-727(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HSP90, IDENTIFICATION IN THE R2PT COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRUVB1_YEAST
AccessioniPrimary (citable) accession number: Q03940
Secondary accession number(s): D6VSH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3