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Q03940 (RUVB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RuvB-like protein 1

Short name=RUVBL1
EC=3.6.4.12
Alternative name(s):
TIP49-homology protein 1
TIP49a homolog
Gene names
Name:RVB1
Synonyms:TIH1, TIP49A
Ordered Locus Names:YDR190C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA helicase which participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes. The SWR1 complex mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. The INO80 complex remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to 'Ser-129' phosphorylated H2A histones as a response to DNA damage. RVB1 recruits ARP5 to the INO80 complex. During transcription may recruit SPT15/TBP to the TATA-boxes of involved genes. Required for box C/D and box H/ACA snoRNA accumulation and involved in pre-rRNA processing. Ref.4 Ref.5 Ref.7 Ref.10 Ref.11 Ref.12

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Probably forms a homohexamer. Interacts with RVB2 and may form heterododecamers with RVB2. Component of the SWR1 chromatin remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80. Component of the R2TP complex composed of at least RVB1, RVB2, TAH1 and PIH1. Interacts with SPT15/TBP and HSP90. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.10 Ref.11 Ref.12 Ref.13

Subcellular location

Nucleus Ref.8.

Miscellaneous

Present with 11600 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RuvB family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 463463RuvB-like protein 1
PRO_0000165661

Regions

Nucleotide binding79 – 868ATP By similarity

Experimental info

Mutagenesis851K → E: Lethal. Ref.5
Mutagenesis3121E → G: Lethal. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q03940 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 93D6ECB7006AF0F2

FASTA46350,453
        10         20         30         40         50         60 
MVAISEVKEN PGVNSSNSGA VTRTAAHTHI KGLGLDESGV AKRVEGGFVG QIEAREACGV 

        70         80         90        100        110        120 
IVDLIKAKKM SGRAILLAGG PSTGKTALAL AISQELGPKV PFCPLVGSEL YSVEVKKTET 

       130        140        150        160        170        180 
LMENFRRAIG LRIKETKEVY EGEVTELTPE DAENPLGGYG KTISHVIVGL KSAKGTKTLR 

       190        200        210        220        230        240 
LDPTIYESIQ REKVSIGDVI YIEANTGAVK RVGRSDAYAT EFDLETEEYV PLPKGEVHKK 

       250        260        270        280        290        300 
KEIVQDVTLH DLDVANARPQ GGQDVISMMG QLLKPKKTEI TEKLRQEVNK VVAKYIDQGV 

       310        320        330        340        350        360 
AELIPGVLFI DEVNMLDIEI FTYLNKALES NIAPVVVLAS NRGMTTVRGT EDVISPHGVP 

       370        380        390        400        410        420 
PDLIDRLLIV RTLPYDKDEI RTIIERRATV ERLQVESSAL DLLATMGTET SLRYALQLLA 

       430        440        450        460 
PCGILAQTSN RKEIVVNDVN EAKLLFLDAK RSTKILETSA NYL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The Saccharomyces cerevisiae RuvB-like protein, Tih2p, is required for cell cycle progression and RNA polymerase II-directed transcription."
Lim C.R., Kimata Y., Ohdate H., Kokubo T., Kikuchi N., Horigome T., Kohno K.
J. Biol. Chem. 275:22409-22417(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RVB2.
[4]"A chromatin remodelling complex involved in transcription and DNA processing."
Shen X., Mizuguchi G., Hamiche A., Wu C.
Nature 406:541-544(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE INO80 COMPLEX, FUNCTION OF THE INO80 COMPLEX, MASS SPECTROMETRY.
[5]"Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes."
Jonsson Z.O., Dhar S.K., Narlikar G.J., Auty R., Wagle N., Pellman D., Pratt R.E., Kingston R., Dutta A.
J. Biol. Chem. 276:16279-16288(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RVB2, IDENTIFICATION IN A COMPLEX WITH RBV2; ACT1 AND ARP4, MASS SPECTROMETRY, MUTAGENESIS OF LYS-85 AND GLU-312.
[6]"Impairment of the DNA binding activity of the TATA-binding protein renders the transcriptional function of Rvb2p/Tih2p, the yeast RuvB-like protein, essential for cell growth."
Ohdate H., Lim C.R., Kokubo T., Matsubara K., Kimata Y., Kohno K.
J. Biol. Chem. 278:14647-14656(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SPT15.
[7]"A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1."
Krogan N.J., Keogh M.-C., Datta N., Sawa C., Ryan O.W., Ding H., Haw R.A., Pootoolal J., Tong A., Canadien V., Richards D.P., Wu X., Emili A., Hughes T.R., Buratowski S., Greenblatt J.F.
Mol. Cell 12:1565-1576(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex."
Jonsson Z.O., Jha S., Wohlschlegel J.A., Dutta A.
Mol. Cell 16:465-477(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE INO80 COMPLEX, MASS SPECTROMETRY.
[11]"A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin."
Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J.
PLoS Biol. 2:587-599(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE SWR1 COMPLEX, MASS SPECTROMETRY.
[12]"ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex."
Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C.
Science 303:343-348(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[13]"Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone."
Zhao R., Davey M.G., Hsu Y.-C., Kaplanek P., Tong A., Parsons A.B., Krogan N.J., Cagney G., Mai D., Greenblatt J.F., Boone C., Emili A., Houry W.A.
Cell 120:715-727(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HSP90, IDENTIFICATION IN THE R2PT COMPLEX, MASS SPECTROMETRY.
[14]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z48784 Genomic DNA. Translation: CAA88704.1.
BK006938 Genomic DNA. Translation: DAA12033.1.
PIRS52698.
RefSeqNP_010476.1. NM_001180498.1.

3D structure databases

ProteinModelPortalQ03940.
SMRQ03940. Positions 23-456.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32243. 155 interactions.
DIPDIP-4896N.
IntActQ03940. 130 interactions.
MINTMINT-523823.
STRING4932.YDR190C.

Proteomic databases

PaxDbQ03940.
PeptideAtlasQ03940.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR190C; YDR190C; YDR190C.
GeneID851771.
KEGGsce:YDR190C.

Organism-specific databases

CYGDYDR190c.
SGDS000002598. RVB1.

Phylogenomic databases

eggNOGCOG1224.
GeneTreeENSGT00550000075043.
HOGENOMHOG000190885.
KOK04499.
OMANKVVSKY.
OrthoDBEOG7FBRT0.

Enzyme and pathway databases

BioCycYEAST:G3O-29778-MONOMER.

Gene expression databases

GenevestigatorQ03940.

Family and domain databases

InterProIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR027238. RuvB-like.
IPR010339. TIP49_C.
[Graphical view]
PANTHERPTHR11093. PTHR11093. 1 hit.
PfamPF06068. TIP49. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

NextBio969564.
PROQ03940.

Entry information

Entry nameRUVB1_YEAST
AccessionPrimary (citable) accession number: Q03940
Secondary accession number(s): D6VSH3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families