RuvB-like protein 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q03940 (RUVB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
RuvB-like protein 1
Short name=RUVBL1
EC=3.6.4.12

Alternative name(s):
TIP49-homology protein 1
TIP49a homolog

Gene names

Name:	RVB1
Synonyms:	TIH1, TIP49A
Ordered Locus Names:	YDR190C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA helicase which participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes. The SWR1 complex mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. The INO80 complex remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to 'Ser-129' phosphorylated H2A histones as a response to DNA damage. RVB1 recruits ARP5 to the INO80 complex. During transcription may recruit SPT15/TBP to the TATA-boxes of involved genes. Required for box C/D and box H/ACA snoRNA accumulation and involved in pre-rRNA processing. Ref.4 Ref.5 Ref.7 Ref.10 Ref.11 Ref.12
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subunit structure	Probably forms a homohexamer. Interacts with RVB2 and may form heterododecamers with RVB2. Component of the SWR1 chromatin remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80. Component of the R2TP complex composed of at least RVB1, RVB2, TAH1 and PIH1. Interacts with SPT15/TBP and HSP90. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.10 Ref.11 Ref.12 Ref.13
Subcellular location	Nucleus Ref.8.
Miscellaneous	Present with 11600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the RuvB family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Chromatin regulator Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW box C/D snoRNP assembly Inferred from mutant phenotype PubMed 18268103. Source: SGD histone exchange Inferred from physical interaction Ref.12. Source: SGD regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.5. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ASTRA complex Inferred from direct assay PubMed 19040720. Source: SGD Ino80 complex Inferred from physical interaction Ref.4. Source: SGD R2TP complex Inferred from direct assay Ref.13. Source: SGD Swr1 complex Inferred from direct assay PubMed 16299513. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent 5'-3' DNA helicase activity Inferred from direct assay PubMed 18234224. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
PIH1	P38768	6	EBI-30712,EBI-24499
RVB2	Q12464	8	EBI-30712,EBI-31814
SPT15	P13393	3	EBI-30712,EBI-19129

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 463

463

RuvB-like protein 1

PRO_0000165661

Regions

Nucleotide binding

79 – 86

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.14

Modified residue

Phosphoserine Ref.14

Experimental info

Mutagenesis

K → E: Lethal. Ref.5

Mutagenesis

312

E → G: Lethal. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

Q03940 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 93D6ECB7006AF0F2
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FASTA

463

50,453

        10         20         30         40         50         60 
MVAISEVKEN PGVNSSNSGA VTRTAAHTHI KGLGLDESGV AKRVEGGFVG QIEAREACGV 

        70         80         90        100        110        120 
IVDLIKAKKM SGRAILLAGG PSTGKTALAL AISQELGPKV PFCPLVGSEL YSVEVKKTET 

       130        140        150        160        170        180 
LMENFRRAIG LRIKETKEVY EGEVTELTPE DAENPLGGYG KTISHVIVGL KSAKGTKTLR 

       190        200        210        220        230        240 
LDPTIYESIQ REKVSIGDVI YIEANTGAVK RVGRSDAYAT EFDLETEEYV PLPKGEVHKK 

       250        260        270        280        290        300 
KEIVQDVTLH DLDVANARPQ GGQDVISMMG QLLKPKKTEI TEKLRQEVNK VVAKYIDQGV 

       310        320        330        340        350        360 
AELIPGVLFI DEVNMLDIEI FTYLNKALES NIAPVVVLAS NRGMTTVRGT EDVISPHGVP 

       370        380        390        400        410        420 
PDLIDRLLIV RTLPYDKDEI RTIIERRATV ERLQVESSAL DLLATMGTET SLRYALQLLA 

       430        440        450        460 
PCGILAQTSN RKEIVVNDVN EAKLLFLDAK RSTKILETSA NYL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The Saccharomyces cerevisiae RuvB-like protein, Tih2p, is required for cell cycle progression and RNA polymerase II-directed transcription." Lim C.R., Kimata Y., Ohdate H., Kokubo T., Kikuchi N., Horigome T., Kohno K. J. Biol. Chem. 275:22409-22417(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RVB2.
[4]	"A chromatin remodelling complex involved in transcription and DNA processing." Shen X., Mizuguchi G., Hamiche A., Wu C. Nature 406:541-544(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE INO80 COMPLEX, FUNCTION OF THE INO80 COMPLEX, MASS SPECTROMETRY.
[5]	"Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes." Jonsson Z.O., Dhar S.K., Narlikar G.J., Auty R., Wagle N., Pellman D., Pratt R.E., Kingston R., Dutta A. J. Biol. Chem. 276:16279-16288(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RVB2, IDENTIFICATION IN A COMPLEX WITH RBV2; ACT1 AND ARP4, MASS SPECTROMETRY, MUTAGENESIS OF LYS-85 AND GLU-312.
[6]	"Impairment of the DNA binding activity of the TATA-binding protein renders the transcriptional function of Rvb2p/Tih2p, the yeast RuvB-like protein, essential for cell growth." Ohdate H., Lim C.R., Kokubo T., Matsubara K., Kimata Y., Kohno K. J. Biol. Chem. 278:14647-14656(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SPT15.
[7]	"A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1." Krogan N.J., Keogh M.-C., Datta N., Sawa C., Ryan O.W., Ding H., Haw R.A., Pootoolal J., Tong A., Canadien V., Richards D.P., Wu X., Emili A., Hughes T.R., Buratowski S., Greenblatt J.F. Mol. Cell 12:1565-1576(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex." Jonsson Z.O., Jha S., Wohlschlegel J.A., Dutta A. Mol. Cell 16:465-477(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE INO80 COMPLEX, MASS SPECTROMETRY.
[11]	"A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin." Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J. PLoS Biol. 2:587-599(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE SWR1 COMPLEX, MASS SPECTROMETRY.
[12]	"ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex." Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C. Science 303:343-348(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[13]	"Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone." Zhao R., Davey M.G., Hsu Y.-C., Kaplanek P., Tong A., Parsons A.B., Krogan N.J., Cagney G., Mai D., Greenblatt J.F., Boone C., Emili A., Houry W.A. Cell 120:715-727(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HSP90, IDENTIFICATION IN THE R2PT COMPLEX, MASS SPECTROMETRY.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-18, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48784 Genomic DNA. Translation: CAA88704.1. BK006938 Genomic DNA. Translation: DAA12033.1.
PIR	S52698.
RefSeq	NP_010476.1. NM_001180498.1.
3D structure databases
ProteinModelPortal	Q03940.
SMR	Q03940. Positions 23-456.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4896N.
IntAct	Q03940. 143 interactions.
MINT	MINT-523823.
STRING	4932.YDR190C.
Proteomic databases
PaxDb	Q03940.
PeptideAtlas	Q03940.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR190C; YDR190C; YDR190C.
GeneID	851771.
KEGG	sce:YDR190C.
Organism-specific databases
CYGD	YDR190c.
SGD	S000002598. RVB1.
Phylogenomic databases
eggNOG	COG1224.
GeneTree	ENSGT00550000075043.
HOGENOM	HOG000190885.
KO	K04499.
OMA	GNKVPFC.
OrthoDB	EOG4QJVWR.
Gene expression databases
Genevestigator	Q03940.
GermOnline	YDR190C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR012340. NA-bd_OB-fold. IPR027238. RuvB-like. IPR010339. TIP49_C. [Graphical view]
PANTHER	PTHR11093. PTHR11093. 1 hit.
Pfam	PF06068. TIP49. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
ProtoNet	Search...
Other
NextBio	969564.

Entry information

Entry name

RUVB1_YEAST

Accession

Primary (citable) accession number: Q03940
Secondary accession number(s): D6VSH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents