Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q03940

- RUVB1_YEAST

UniProt

Q03940 - RUVB1_YEAST

Protein

RuvB-like protein 1

Gene

RVB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    DNA helicase which participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes. The SWR1 complex mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. The INO80 complex remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to 'Ser-129' phosphorylated H2A histones as a response to DNA damage. RVB1 recruits ARP5 to the INO80 complex. During transcription may recruit SPT15/TBP to the TATA-boxes of involved genes. Required for box C/D and box H/ACA snoRNA accumulation and involved in pre-rRNA processing.6 Publications

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi79 – 868ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent 5'-3' DNA helicase activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. box C/D snoRNP assembly Source: SGD
    2. chromatin remodeling Source: SGD
    3. DNA duplex unwinding Source: GOC
    4. DNA repair Source: UniProtKB-KW
    5. histone exchange Source: SGD
    6. regulation of transcription from RNA polymerase II promoter Source: SGD
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Chromatin regulator, Helicase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29778-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RuvB-like protein 1 (EC:3.6.4.12)
    Short name:
    RUVBL1
    Alternative name(s):
    TIP49-homology protein 1
    TIP49a homolog
    Gene namesi
    Name:RVB1
    Synonyms:TIH1, TIP49A
    Ordered Locus Names:YDR190C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR190c.
    SGDiS000002598. RVB1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. Ino80 complex Source: SGD
    2. nucleus Source: SGD
    3. R2TP complex Source: SGD
    4. Swr1 complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi85 – 851K → E: Lethal. 1 Publication
    Mutagenesisi312 – 3121E → G: Lethal. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 463463RuvB-like protein 1PRO_0000165661Add
    BLAST

    Proteomic databases

    MaxQBiQ03940.
    PaxDbiQ03940.
    PeptideAtlasiQ03940.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03940.

    Interactioni

    Subunit structurei

    Probably forms a homohexamer. Interacts with RVB2 and may form heterododecamers with RVB2. Component of the SWR1 chromatin remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80. Component of the R2TP complex composed of at least RVB1, RVB2, TAH1 and PIH1. Interacts with SPT15/TBP and HSP90.9 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PIH1P387686EBI-30712,EBI-24499
    RVB2Q1246410EBI-30712,EBI-31814
    SPT15P133933EBI-30712,EBI-19129

    Protein-protein interaction databases

    BioGridi32243. 155 interactions.
    DIPiDIP-4896N.
    IntActiQ03940. 130 interactions.
    MINTiMINT-523823.
    STRINGi4932.YDR190C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03940.
    SMRiQ03940. Positions 23-456.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvB family.Curated

    Phylogenomic databases

    eggNOGiCOG1224.
    GeneTreeiENSGT00550000075043.
    HOGENOMiHOG000190885.
    KOiK04499.
    OMAiNKVVSKY.
    OrthoDBiEOG7FBRT0.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR027417. P-loop_NTPase.
    IPR027238. RuvB-like.
    IPR010339. TIP49_C.
    [Graphical view]
    PANTHERiPTHR11093. PTHR11093. 1 hit.
    PfamiPF06068. TIP49. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q03940-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAISEVKEN PGVNSSNSGA VTRTAAHTHI KGLGLDESGV AKRVEGGFVG    50
    QIEAREACGV IVDLIKAKKM SGRAILLAGG PSTGKTALAL AISQELGPKV 100
    PFCPLVGSEL YSVEVKKTET LMENFRRAIG LRIKETKEVY EGEVTELTPE 150
    DAENPLGGYG KTISHVIVGL KSAKGTKTLR LDPTIYESIQ REKVSIGDVI 200
    YIEANTGAVK RVGRSDAYAT EFDLETEEYV PLPKGEVHKK KEIVQDVTLH 250
    DLDVANARPQ GGQDVISMMG QLLKPKKTEI TEKLRQEVNK VVAKYIDQGV 300
    AELIPGVLFI DEVNMLDIEI FTYLNKALES NIAPVVVLAS NRGMTTVRGT 350
    EDVISPHGVP PDLIDRLLIV RTLPYDKDEI RTIIERRATV ERLQVESSAL 400
    DLLATMGTET SLRYALQLLA PCGILAQTSN RKEIVVNDVN EAKLLFLDAK 450
    RSTKILETSA NYL 463
    Length:463
    Mass (Da):50,453
    Last modified:November 1, 1996 - v1
    Checksum:i93D6ECB7006AF0F2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48784 Genomic DNA. Translation: CAA88704.1.
    BK006938 Genomic DNA. Translation: DAA12033.1.
    PIRiS52698.
    RefSeqiNP_010476.1. NM_001180498.1.

    Genome annotation databases

    EnsemblFungiiYDR190C; YDR190C; YDR190C.
    GeneIDi851771.
    KEGGisce:YDR190C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48784 Genomic DNA. Translation: CAA88704.1 .
    BK006938 Genomic DNA. Translation: DAA12033.1 .
    PIRi S52698.
    RefSeqi NP_010476.1. NM_001180498.1.

    3D structure databases

    ProteinModelPortali Q03940.
    SMRi Q03940. Positions 23-456.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32243. 155 interactions.
    DIPi DIP-4896N.
    IntActi Q03940. 130 interactions.
    MINTi MINT-523823.
    STRINGi 4932.YDR190C.

    Proteomic databases

    MaxQBi Q03940.
    PaxDbi Q03940.
    PeptideAtlasi Q03940.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR190C ; YDR190C ; YDR190C .
    GeneIDi 851771.
    KEGGi sce:YDR190C.

    Organism-specific databases

    CYGDi YDR190c.
    SGDi S000002598. RVB1.

    Phylogenomic databases

    eggNOGi COG1224.
    GeneTreei ENSGT00550000075043.
    HOGENOMi HOG000190885.
    KOi K04499.
    OMAi NKVVSKY.
    OrthoDBi EOG7FBRT0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29778-MONOMER.

    Miscellaneous databases

    NextBioi 969564.
    PROi Q03940.

    Gene expression databases

    Genevestigatori Q03940.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR027417. P-loop_NTPase.
    IPR027238. RuvB-like.
    IPR010339. TIP49_C.
    [Graphical view ]
    PANTHERi PTHR11093. PTHR11093. 1 hit.
    Pfami PF06068. TIP49. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The Saccharomyces cerevisiae RuvB-like protein, Tih2p, is required for cell cycle progression and RNA polymerase II-directed transcription."
      Lim C.R., Kimata Y., Ohdate H., Kokubo T., Kikuchi N., Horigome T., Kohno K.
      J. Biol. Chem. 275:22409-22417(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RVB2.
    4. "A chromatin remodelling complex involved in transcription and DNA processing."
      Shen X., Mizuguchi G., Hamiche A., Wu C.
      Nature 406:541-544(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE INO80 COMPLEX, FUNCTION OF THE INO80 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes."
      Jonsson Z.O., Dhar S.K., Narlikar G.J., Auty R., Wagle N., Pellman D., Pratt R.E., Kingston R., Dutta A.
      J. Biol. Chem. 276:16279-16288(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RVB2, IDENTIFICATION IN A COMPLEX WITH RBV2; ACT1 AND ARP4, IDENTIFICATION BY MASS SPECTROMETRY, MUTAGENESIS OF LYS-85 AND GLU-312.
    6. "Impairment of the DNA binding activity of the TATA-binding protein renders the transcriptional function of Rvb2p/Tih2p, the yeast RuvB-like protein, essential for cell growth."
      Ohdate H., Lim C.R., Kokubo T., Matsubara K., Kimata Y., Kohno K.
      J. Biol. Chem. 278:14647-14656(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SPT15.
    7. Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex."
      Jonsson Z.O., Jha S., Wohlschlegel J.A., Dutta A.
      Mol. Cell 16:465-477(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE INO80 COMPLEX, IDENTIFICATION BY IDENTIFICATION BY MASS SPECTROMETRY.
    11. "A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin."
      Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J.
      PLoS Biol. 2:587-599(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    12. "ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex."
      Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C.
      Science 303:343-348(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    13. "Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone."
      Zhao R., Davey M.G., Hsu Y.-C., Kaplanek P., Tong A., Parsons A.B., Krogan N.J., Cagney G., Mai D., Greenblatt J.F., Boone C., Emili A., Houry W.A.
      Cell 120:715-727(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HSP90, IDENTIFICATION IN THE R2PT COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRUVB1_YEAST
    AccessioniPrimary (citable) accession number: Q03940
    Secondary accession number(s): D6VSH3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 11600 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3