Q03935 (YAP6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-1-like transcription factor YAP6

Gene names

Name:	YAP6
Synonyms:	HAL7
Ordered Locus Names:	YDR259C
ORF Names:	YD9320A.09C, YD9320A.10C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription activator involved in the regulation of genes expressed in response to environmental changes and metabolic requirements. According to genome-wide promoter binding and gene expression studies it regulates, among others, genes involved in ribosome biogenesis, protein synthesis, carbohydrate metabolism, and carbohydrate transport. It may also be involved in pleiotropic drug resistance. When overexpressed, it confers resistance to cisplatin, methylmethanosulfonate, and mitomycin C, and increases cellular tolerance to sodium and lithium. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Subunit structure	Homodimer By similarity.
Subcellular location	Nucleus Ref.6.
Induction	Seems to activate its own expression and that of the repressor ROX1 which in turn represses YAP6 expression.
Miscellaneous	One of 8 closely related fungi-specific YAP proteins (YAP1 to YAP8), which all seem to be transcription activators of the environmental stress response and metabolism control pathways and to have similar but not identical DNA binding specificities. Present with 1400 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the bZIP family. YAP subfamily. Contains 1 bZIP domain.
Sequence caution	The sequence CAA92717.1 differs from that shown. Reason: Frameshift at position 244.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD
Cellular component	nucleus Inferred from direct assay Ref.6. Source: SGD
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from direct assay. Source: SGD sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	AP-1-like transcription factor YAP6		PRO_0000076526
Regions
	Domain	249 – 280	32	Leucine-zipper
	DNA binding	222 – 245	24	Basic motif
	Compositional bias	263 – 333	71	Asn-rich

Sequences

	Sequence		Length	Mass (Da)
	Q03935 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 396C20006DEB7319	FASTA	383	43,597
	10 20 30 40 50 60 MQNPPLIRPD MYNQGSSSMA TYNASEKNLN EHPSPQIAQP STSQKLPYRI NPTTTNGDTD 70 80 90 100 110 120 ISVNSNPIQP PLPNLMHLSG PSDYRSMHQS PIHPSYIIPP HSNERKQSAS YNRPQNAHVS 130 140 150 160 170 180 IQPSVVFPPK SYSISYAPYQ INPPLPNGLP NQSISLNKEY IAEEQLSTLP SRNTSVTTAP 190 200 210 220 230 240 PSFQNSADTA KNSADNNDNN DNVTKPVPDK DTQLISSSGK TLRNTRRAAQ NRTAQKAFRQ 250 260 270 280 290 300 RKEKYIKNLE QKSKIFDDLL AENNNFKSLN DSLRNDNNIL IAQHEAIRNA ITMLRSEYDV 310 320 330 340 350 360 LCNENNMLKN ENSIIKNEHN MSRNENENLK LENKRFHAEY IRMIEDIENT KRKEQEQRDE 370 380 IEQLKKKIRS LEEIVGRHSD SAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions." Fernandes L., Rodrigues-Pousada C., Struhl K. Mol. Cell. Biol. 17:6982-6993(1997) [PubMed: 9372930] [Abstract] Cited for: FUNCTION, ISOLATION OF YAP FAMILY PROTEINS.
[5]	"Yeast putative transcription factors involved in salt tolerance." Mendizabal I., Rios G., Mulet J.M., Serrano R., de Larrinoa I.F. FEBS Lett. 425:323-328(1998) [PubMed: 9559673] [Abstract] Cited for: FUNCTION, SALT TOLERANCE.
[6]	"Two nuclear proteins, Cin5 and Ydr259c, confer resistance to cisplatin in Saccharomyces cerevisiae." Furuchi T., Ishikawa H., Miura N., Ishizuka M., Kajiya K., Kuge S., Naganuma A. Mol. Pharmacol. 59:470-474(2001) [PubMed: 11179441] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PLEIOTROPIC DRUG-RESISTANCE.
[7]	"Transcriptional regulatory networks in Saccharomyces cerevisiae." Lee T.I., Rinaldi N.J., Robert F., Odom D.T., Bar-Joseph Z., Gerber G.K., Hannett N.M., Harbison C.T., Thompson C.M., Simon I., Zeitlinger J., Jennings E.G., Murray H.L., Gordon D.B., Ren B., Wyrick J.J., Tagne J.B., Volkert T.L. , Fraenkel E., Gifford D.K., Young R.A. Science 298:799-804(2002) [PubMed: 12399584] [Abstract] Cited for: FUNCTION, IDENTIFICATION OF TARGET PROMOTERS.
[8]	"Computational discovery of gene modules and regulatory networks." Bar-Joseph Z., Gerber G.K., Lee T.I., Rinaldi N.J., Yoo J.Y., Robert F., Gordon D.B., Fraenkel E., Jaakkola T.S., Young R.A., Gifford D.K. Nat. Biotechnol. 21:1337-1342(2003) [PubMed: 14555958] [Abstract] Cited for: FUNCTION, REGULATORY TRANSCRIPTION MODULES.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70202 Genomic DNA. Translation: CAA94098.1. Z68329 Genomic DNA. Translation: CAA92716.1. Z68329 Genomic DNA. Translation: CAA92717.1. Frameshift. AY557790 Genomic DNA. Translation: AAS56116.1. BK006938 Genomic DNA. Translation: DAA12099.1.
PIR	S67462.
RefSeq	NP_010545.1. NM_001180567.1.
3D structure databases
ProteinModelPortal	Q03935.
SMR	Q03935. Positions 331-373.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4253N.
IntAct	Q03935. 9 interactions.
MINT	MINT-511437.
STRING	Q03935.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR259C; YDR259C; YDR259C.
GeneID	851846.
KEGG	sce:YDR259C.
NMPDR	fig|4932.3.peg.1303.
Organism-specific databases
CYGD	YDR259c.
SGD	S000002667. YAP6.
Phylogenomic databases
eggNOG	euNOG11886.
GeneTree	EFGT00050000004237.
OrthoDB	EOG4TXG2M.
Gene expression databases
ArrayExpress	Q03935.
Genevestigator	Q03935.
GermOnline	YDR259C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004827. bZIP. IPR011616. bZIP_1. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. False negative. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969760.

Entry information

Entry name

YAP6_YEAST

Accession

Primary (citable) accession number: Q03935
Secondary accession number(s): D6VSN9, Q03926, Q03931

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program