Reviewed, UniProtKB/Swiss-Prot Q03933 (HSF2_HUMAN)

Names and origin

Protein names

Recommended name:
    Heat shock factor protein 2
      Short name=HSF 2
Alternative name(s):
    Heat shock transcription factor 2
      Short name=HSTF 2

Gene names

Name:	HSF2
Synonyms:	HSTF2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Subunit structure	DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.
Subcellular location	Cytoplasm. Nucleus. Note: Cytoplasmic during normal growth and moves to the nucleus upon activation. Ref.5
Sequence similarities	Belongs to the HSF family.

Ontologies

Keywords
Biological process	Stress response Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription coactivator activity Ref.1 Traceable author statement. Source: ProtInc transcription factor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Heat shock factor protein 2		PRO_0000124569
Regions
	DNA binding	7 – 112	106	By similarity
	Region	119 – 192	74	Hydrophobic repeat HR-A/B
	Region	360 – 385	26	Hydrophobic repeat HR-C
	Motif	108 – 122	15	Nuclear localization signal Potential
	Motif	195 – 210	16	Nuclear localization signal Potential
	Compositional bias	170 – 173	4	Poly-Gln
Experimental info
	Mutagenesis	109	1	R → G: Fails to translocate to nucleus.
	Mutagenesis	196 – 198	3	RKR → ASS: Fails to translocate to nucleus.

Sequences

	Sequence		Length	Mass (Da)
	Q03933-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 00DFD05CFD9DF0D3	FASTA	536	60,348
	10 20 30 40 50 60 MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP KYFKHNNMAS 70 80 90 100 110 120 FVRQLNMYGF RKVVHIDSGI VKQERDGPVE FQHPYFKQGQ DDLLENIKRK VSSSKPEENK 130 140 150 160 170 180 IRQEDLTKII SSAQKVQIKQ ETIESRLSEL KSENESLWKE VSELRAKHAQ QQQVIRKIVQ 190 200 210 220 230 240 FIVTLVQNNQ LVSLKRKRPL LLNTNGAQKK NLFQHIVKEP TDNHHHKVPH SRTEGLKPRE 250 260 270 280 290 300 RISDDIIIYD VTDDNADEEN IPVIPETNED VISDPSNCSQ YPDIVIVEDD NEDEYAPVIQ 310 320 330 340 350 360 SGEQNEPARE SLSSGSDGSS PLMSSAVQLN GSSSLTSEDP VTMMDSILND NINLLGKVEL 370 380 390 400 410 420 LDYLDSIDCS LEDFQAMLSG RQFSIDPDLL VDLFTSSVQM NPTDYINNTK SENKGLETTK 430 440 450 460 470 480 NNVVQPVSEE GRKSKSKPDK QLIQYTAFPL LAFLDGNPAS SVEQASTTAS SEVLSSVDKP 490 500 510 520 530 IEVDELLDSS LDPEPTQSKL VRLEPLTEAE ASEATLFYLC ELAPAPLDSD MPLLDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans." Schuetz T.J., Gallo G.J., Sheldon L., Tempst P., Kingston R.E. Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915(1991) [PubMed: 1871106] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 65-71.
[2]	NIEHS SNPs program Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Hydrophobic coiled-coil domains regulate the subcellular localization of human heat shock factor 2." Sheldon L., Kingston R.E. Genes Dev. 7:1549-1558(1993) [PubMed: 8339932] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	M65217 mRNA. Translation: AAA36017.1. DQ492684 Genomic DNA. Translation: ABF47087.1. AL121954, Z99129 Genomic DNA. Translation: CAC14262.2. Z99129, AL121954 Genomic DNA. Translation: CAI20560.1. BC112323 mRNA. Translation: AAI12324.1. BC121051 mRNA. Translation: AAI21052.1.
IPI	IPI00012878.
PIR	A41138.
RefSeq	NP_004497.1.
UniGene	Hs.158195
3D structure databases
HSSP	HSSP built from PDB template 3HTS based on UniProtKB P22121.
ModBase	Search...
PTM databases
PhosphoSite	Q03933.
Proteomic databases
PRIDE	Q03933.
Genome annotation databases
Ensembl	ENSG00000025156. Homo sapiens. [Contig view]
GeneID	3298.
Organism-specific databases
GeneCards	GC06P122762.
HGNC	HGNC:5225. HSF2.
HPA	CAB016067.
MIM	140581. gene.
PharmGKB	PA29494.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q03933.
HOVERGEN	Q03933.
OMA	Q03933. NCSQYPD.
Gene expression databases
ArrayExpress	Q03933.
Bgee	Q03933.
CleanEx	HS_HSF2.
GermOnline	ENSG00000025156. Homo sapiens.
Family and domain databases
InterPro	IPR000232. HSF_DNA_bd. IPR010542. Vert_HS_TF. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00447. HSF_DNA-bind. 1 hit. PF06546. Vert_HS_TF. 1 hit. [Graphical view]
PRINTS	PR00056. HSFDOMAIN.
ProDom	PD001788. HSF_DNA_bind. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00415. HSF. 1 hit. [Graphical view]
PROSITE	PS00434. HSF_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	13083.
SOURCE	Search...

Entry information

Entry name

HSF2_HUMAN

Accession

Primary (citable) accession number: Q03933
Secondary accession number(s): Q2M1K4, Q9H445

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)