Q03933 (HSF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heat shock factor protein 2
Short name=HSF 2

Alternative name(s):
Heat shock transcription factor 2
Short name=HSTF 2

Gene names

Name:	HSF2
Synonyms:	HSTF2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Subunit structure	DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.
Subcellular location	Cytoplasm. Nucleus. Note: Cytoplasmic during normal growth and moves to the nucleus upon activation. Ref.7
Sequence similarities	Belongs to the HSF family.

Ontologies

Keywords
Biological process	Stress response Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 10561509. Source: MGI response to stress Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from electronic annotation. Source: Compara transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA
Molecular_function	RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding Inferred from direct assay PubMed 10561509. Source: MGI sequence-specific DNA binding transcription factor activity Traceable author statement Ref.1. Source: ProtInc transcription coactivator activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q03933-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q03933-2)

The sequence of this isoform differs from the canonical sequence as follows:
     393-410: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Heat shock factor protein 2		PRO_0000124569
Regions
	DNA binding	7 – 112	106	By similarity
	Region	119 – 192	74	Hydrophobic repeat HR-A/B
	Region	360 – 385	26	Hydrophobic repeat HR-C
	Motif	108 – 122	15	Nuclear localization signal Potential
	Motif	195 – 210	16	Nuclear localization signal Potential
	Compositional bias	170 – 173	4	Poly-Gln
Natural variations
	Alternative sequence	393 – 410	18	Missing in isoform 2.		VSP_041128
Experimental info
	Mutagenesis	109	1	R → G: Fails to translocate to nucleus.
	Mutagenesis	196 – 198	3	RKR → ASS: Fails to translocate to nucleus.

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 00DFD05CFD9DF0D3	FASTA	536	60,348
	10 20 30 40 50 60 MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP KYFKHNNMAS 70 80 90 100 110 120 FVRQLNMYGF RKVVHIDSGI VKQERDGPVE FQHPYFKQGQ DDLLENIKRK VSSSKPEENK 130 140 150 160 170 180 IRQEDLTKII SSAQKVQIKQ ETIESRLSEL KSENESLWKE VSELRAKHAQ QQQVIRKIVQ 190 200 210 220 230 240 FIVTLVQNNQ LVSLKRKRPL LLNTNGAQKK NLFQHIVKEP TDNHHHKVPH SRTEGLKPRE 250 260 270 280 290 300 RISDDIIIYD VTDDNADEEN IPVIPETNED VISDPSNCSQ YPDIVIVEDD NEDEYAPVIQ 310 320 330 340 350 360 SGEQNEPARE SLSSGSDGSS PLMSSAVQLN GSSSLTSEDP VTMMDSILND NINLLGKVEL 370 380 390 400 410 420 LDYLDSIDCS LEDFQAMLSG RQFSIDPDLL VDLFTSSVQM NPTDYINNTK SENKGLETTK 430 440 450 460 470 480 NNVVQPVSEE GRKSKSKPDK QLIQYTAFPL LAFLDGNPAS SVEQASTTAS SEVLSSVDKP 490 500 510 520 530 IEVDELLDSS LDPEPTQSKL VRLEPLTEAE ASEATLFYLC ELAPAPLDSD MPLLDS
	Isoform 2 [UniParc]. Checksum: 6682B51448E116BC	FASTA	518	58,293
	10 20 30 40 50 60 MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP KYFKHNNMAS 70 80 90 100 110 120 FVRQLNMYGF RKVVHIDSGI VKQERDGPVE FQHPYFKQGQ DDLLENIKRK VSSSKPEENK 130 140 150 160 170 180 IRQEDLTKII SSAQKVQIKQ ETIESRLSEL KSENESLWKE VSELRAKHAQ QQQVIRKIVQ 190 200 210 220 230 240 FIVTLVQNNQ LVSLKRKRPL LLNTNGAQKK NLFQHIVKEP TDNHHHKVPH SRTEGLKPRE 250 260 270 280 290 300 RISDDIIIYD VTDDNADEEN IPVIPETNED VISDPSNCSQ YPDIVIVEDD NEDEYAPVIQ 310 320 330 340 350 360 SGEQNEPARE SLSSGSDGSS PLMSSAVQLN GSSSLTSEDP VTMMDSILND NINLLGKVEL 370 380 390 400 410 420 LDYLDSIDCS LEDFQAMLSG RQFSIDPDLL VDSENKGLET TKNNVVQPVS EEGRKSKSKP 430 440 450 460 470 480 DKQLIQYTAF PLLAFLDGNP ASSVEQASTT ASSEVLSSVD KPIEVDELLD SSLDPEPTQS 490 500 510 KLVRLEPLTE AEASEATLFY LCELAPAPLD SDMPLLDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans." Schuetz T.J., Gallo G.J., Sheldon L., Tempst P., Kingston R.E. Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 65-71.
[2]	NIEHS SNPs program Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[7]	"Hydrophobic coiled-coil domains regulate the subcellular localization of human heat shock factor 2." Sheldon L., Kingston R.E. Genes Dev. 7:1549-1558(1993) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	M65217 mRNA. Translation: AAA36017.1. DQ492684 Genomic DNA. Translation: ABF47087.1. AK294624 mRNA. Translation: BAG57805.1. AK316377 mRNA. Translation: BAH14748.1. AL121954, Z99129 Genomic DNA. Translation: CAC14262.2. Z99129, AL121954 Genomic DNA. Translation: CAI20560.1. CH471051 Genomic DNA. Translation: EAW48176.1. BC112323 mRNA. Translation: AAI12324.1. BC121050 mRNA. Translation: AAI21051.1. BC121051 mRNA. Translation: AAI21052.1. BC128420 mRNA. Translation: AAI28421.1.
IPI	IPI00012878. IPI00913872.
PIR	A41138.
RefSeq	NP_001129036.1. NM_001135564.1. NP_004497.1. NM_004506.3.
UniGene	Hs.158195.
3D structure databases
ProteinModelPortal	Q03933.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03933. 2 interactions.
STRING	9606.ENSP00000357440.
PTM databases
PhosphoSite	Q03933.
Polymorphism databases
DMDM	462334.
Proteomic databases
PaxDb	Q03933.
PRIDE	Q03933.
Protocols and materials databases
DNASU	3298.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000368455; ENSP00000357440; ENSG00000025156. ENST00000452194; ENSP00000400380; ENSG00000025156.
GeneID	3298.
KEGG	hsa:3298.
UCSC	uc003pyu.2. human. uc003pyv.2. human.
Organism-specific databases
CTD	3298.
GeneCards	GC06P122762.
HGNC	HGNC:5225. HSF2.
HPA	CAB016067. HPA031455.
MIM	140581. gene.
neXtProt	NX_Q03933.
PharmGKB	PA29494.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5169.
HOGENOM	HOG000253917.
HOVERGEN	HBG005999.
InParanoid	Q03933.
KO	K09415.
OMA	QNEPARE.
OrthoDB	EOG4V4382.
PhylomeDB	Q03933.
Gene expression databases
Bgee	Q03933.
CleanEx	HS_HSF2.
Genevestigator	Q03933.
GermOnline	ENSG00000025156. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR000232. HSF_DNA-bd. IPR010542. Vert_HS_TF. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00447. HSF_DNA-bind. 1 hit. PF06546. Vert_HS_TF. 1 hit. [Graphical view]
PRINTS	PR00056. HSFDOMAIN.
SMART	SM00415. HSF. 1 hit. [Graphical view]
PROSITE	PS00434. HSF_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	3298.
NextBio	13083.
SOURCE	Search...

Entry information

Entry name

HSF2_HUMAN

Accession

Primary (citable) accession number: Q03933
Secondary accession number(s): B4DGJ4 , Q0VAH9, Q2M1K4, Q9H445

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.