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Protein

Heat shock factor protein 2

Gene

HSF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi7 – 112By similarityAdd BLAST106

GO - Molecular functioni

GO - Biological processi

  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • spermatogenesis Source: Ensembl
  • transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000025156-MONOMER.
SignaLinkiQ03933.
SIGNORiQ03933.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock factor protein 2
Short name:
HSF 2
Alternative name(s):
Heat shock transcription factor 2
Short name:
HSTF 2
Gene namesi
Name:HSF2
Synonyms:HSTF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:5225. HSF2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Cytoplasmic during normal growth and moves to the nucleus upon activation.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi109R → G: Fails to translocate to nucleus. 1 Publication1
Mutagenesisi196 – 198RKR → ASS: Fails to translocate to nucleus. 1 Publication3

Organism-specific databases

DisGeNETi3298.
OpenTargetsiENSG00000025156.
PharmGKBiPA29494.

Polymorphism and mutation databases

BioMutaiHSF2.
DMDMi462334.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001245691 – 536Heat shock factor protein 2Add BLAST536

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki82Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki139Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ03933.
MaxQBiQ03933.
PaxDbiQ03933.
PeptideAtlasiQ03933.
PRIDEiQ03933.

PTM databases

iPTMnetiQ03933.
PhosphoSitePlusiQ03933.

Expressioni

Gene expression databases

BgeeiENSG00000025156.
CleanExiHS_HSF2.
ExpressionAtlasiQ03933. baseline and differential.
GenevisibleiQ03933. HS.

Organism-specific databases

HPAiCAB016067.
HPA031455.

Interactioni

Subunit structurei

DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
MTUS2Q5JR593EBI-10223348,EBI-742948
NUP62P371983EBI-10223348,EBI-347978
TPM3P067535EBI-2556750,EBI-355607
TPM3Q5VU623EBI-2556750,EBI-10184033
WDR5P619645EBI-2556750,EBI-540834

Protein-protein interaction databases

BioGridi109531. 41 interactors.
DIPiDIP-56593N.
IntActiQ03933. 64 interactors.
STRINGi9606.ENSP00000357440.

Structurei

Secondary structure

1536
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 19Combined sources11
Helixi21 – 23Combined sources3
Turni24 – 26Combined sources3
Beta strandi27 – 29Combined sources3
Beta strandi33 – 39Combined sources7
Helixi41 – 47Combined sources7
Helixi49 – 52Combined sources4
Helixi58 – 67Combined sources10
Beta strandi78 – 80Combined sources3
Beta strandi83 – 85Combined sources3
Beta strandi89 – 92Combined sources4
Helixi101 – 106Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5D8KX-ray1.73B8-115[»]
5D8LX-ray2.07B/D/F/H8-115[»]
ProteinModelPortaliQ03933.
SMRiQ03933.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 192Hydrophobic repeat HR-A/BAdd BLAST74
Regioni360 – 385Hydrophobic repeat HR-CAdd BLAST26

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi108 – 122Nuclear localization signalSequence analysisAdd BLAST15
Motifi195 – 210Nuclear localization signalSequence analysisAdd BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi170 – 173Poly-Gln4

Sequence similaritiesi

Belongs to the HSF family.Curated

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiQ03933.
KOiK09415.
OMAiISDPSNC.
OrthoDBiEOG091G087O.
PhylomeDBiQ03933.
TreeFamiTF330401.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027712. HSF2.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR010542. Vert_HSTF_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF185. PTHR10015:SF185. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF06546. Vert_HS_TF. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q03933-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP
60 70 80 90 100
KYFKHNNMAS FVRQLNMYGF RKVVHIDSGI VKQERDGPVE FQHPYFKQGQ
110 120 130 140 150
DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ ETIESRLSEL
160 170 180 190 200
KSENESLWKE VSELRAKHAQ QQQVIRKIVQ FIVTLVQNNQ LVSLKRKRPL
210 220 230 240 250
LLNTNGAQKK NLFQHIVKEP TDNHHHKVPH SRTEGLKPRE RISDDIIIYD
260 270 280 290 300
VTDDNADEEN IPVIPETNED VISDPSNCSQ YPDIVIVEDD NEDEYAPVIQ
310 320 330 340 350
SGEQNEPARE SLSSGSDGSS PLMSSAVQLN GSSSLTSEDP VTMMDSILND
360 370 380 390 400
NINLLGKVEL LDYLDSIDCS LEDFQAMLSG RQFSIDPDLL VDLFTSSVQM
410 420 430 440 450
NPTDYINNTK SENKGLETTK NNVVQPVSEE GRKSKSKPDK QLIQYTAFPL
460 470 480 490 500
LAFLDGNPAS SVEQASTTAS SEVLSSVDKP IEVDELLDSS LDPEPTQSKL
510 520 530
VRLEPLTEAE ASEATLFYLC ELAPAPLDSD MPLLDS
Length:536
Mass (Da):60,348
Last modified:February 1, 1994 - v1
Checksum:i00DFD05CFD9DF0D3
GO
Isoform 2 (identifier: Q03933-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     393-410: Missing.

Show »
Length:518
Mass (Da):58,293
Checksum:i6682B51448E116BC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041128393 – 410Missing in isoform 2. 2 PublicationsAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65217 mRNA. Translation: AAA36017.1.
DQ492684 Genomic DNA. Translation: ABF47087.1.
AK294624 mRNA. Translation: BAG57805.1.
AK316377 mRNA. Translation: BAH14748.1.
AL121954, Z99129 Genomic DNA. Translation: CAC14262.2.
Z99129, AL121954 Genomic DNA. Translation: CAI20560.1.
CH471051 Genomic DNA. Translation: EAW48176.1.
BC112323 mRNA. Translation: AAI12324.1.
BC121050 mRNA. Translation: AAI21051.1.
BC121051 mRNA. Translation: AAI21052.1.
BC128420 mRNA. Translation: AAI28421.1.
CCDSiCCDS47470.1. [Q03933-2]
CCDS5124.1. [Q03933-1]
PIRiA41138.
RefSeqiNP_001129036.1. NM_001135564.1. [Q03933-2]
NP_004497.1. NM_004506.3. [Q03933-1]
UniGeneiHs.158195.

Genome annotation databases

EnsembliENST00000368455; ENSP00000357440; ENSG00000025156. [Q03933-1]
ENST00000452194; ENSP00000400380; ENSG00000025156. [Q03933-2]
GeneIDi3298.
KEGGihsa:3298.
UCSCiuc003pyu.3. human. [Q03933-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65217 mRNA. Translation: AAA36017.1.
DQ492684 Genomic DNA. Translation: ABF47087.1.
AK294624 mRNA. Translation: BAG57805.1.
AK316377 mRNA. Translation: BAH14748.1.
AL121954, Z99129 Genomic DNA. Translation: CAC14262.2.
Z99129, AL121954 Genomic DNA. Translation: CAI20560.1.
CH471051 Genomic DNA. Translation: EAW48176.1.
BC112323 mRNA. Translation: AAI12324.1.
BC121050 mRNA. Translation: AAI21051.1.
BC121051 mRNA. Translation: AAI21052.1.
BC128420 mRNA. Translation: AAI28421.1.
CCDSiCCDS47470.1. [Q03933-2]
CCDS5124.1. [Q03933-1]
PIRiA41138.
RefSeqiNP_001129036.1. NM_001135564.1. [Q03933-2]
NP_004497.1. NM_004506.3. [Q03933-1]
UniGeneiHs.158195.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5D8KX-ray1.73B8-115[»]
5D8LX-ray2.07B/D/F/H8-115[»]
ProteinModelPortaliQ03933.
SMRiQ03933.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109531. 41 interactors.
DIPiDIP-56593N.
IntActiQ03933. 64 interactors.
STRINGi9606.ENSP00000357440.

PTM databases

iPTMnetiQ03933.
PhosphoSitePlusiQ03933.

Polymorphism and mutation databases

BioMutaiHSF2.
DMDMi462334.

Proteomic databases

EPDiQ03933.
MaxQBiQ03933.
PaxDbiQ03933.
PeptideAtlasiQ03933.
PRIDEiQ03933.

Protocols and materials databases

DNASUi3298.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368455; ENSP00000357440; ENSG00000025156. [Q03933-1]
ENST00000452194; ENSP00000400380; ENSG00000025156. [Q03933-2]
GeneIDi3298.
KEGGihsa:3298.
UCSCiuc003pyu.3. human. [Q03933-1]

Organism-specific databases

CTDi3298.
DisGeNETi3298.
GeneCardsiHSF2.
HGNCiHGNC:5225. HSF2.
HPAiCAB016067.
HPA031455.
MIMi140581. gene.
neXtProtiNX_Q03933.
OpenTargetsiENSG00000025156.
PharmGKBiPA29494.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiQ03933.
KOiK09415.
OMAiISDPSNC.
OrthoDBiEOG091G087O.
PhylomeDBiQ03933.
TreeFamiTF330401.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000025156-MONOMER.
SignaLinkiQ03933.
SIGNORiQ03933.

Miscellaneous databases

GeneWikiiHSF2.
GenomeRNAii3298.
PROiQ03933.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000025156.
CleanExiHS_HSF2.
ExpressionAtlasiQ03933. baseline and differential.
GenevisibleiQ03933. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027712. HSF2.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR010542. Vert_HSTF_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF185. PTHR10015:SF185. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF06546. Vert_HS_TF. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSF2_HUMAN
AccessioniPrimary (citable) accession number: Q03933
Secondary accession number(s): B4DGJ4
, Q0VAH9, Q2M1K4, Q9H445
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.