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Protein

Heat shock factor protein 2

Gene

HSF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi7 – 112106By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • spermatogenesis Source: Ensembl
  • transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ03933.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock factor protein 2
Short name:
HSF 2
Alternative name(s):
Heat shock transcription factor 2
Short name:
HSTF 2
Gene namesi
Name:HSF2
Synonyms:HSTF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:5225. HSF2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Cytoplasmic during normal growth and moves to the nucleus upon activation.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi109 – 1091R → G: Fails to translocate to nucleus. 1 Publication
Mutagenesisi196 – 1983RKR → ASS: Fails to translocate to nucleus. 1 Publication

Organism-specific databases

PharmGKBiPA29494.

Polymorphism and mutation databases

BioMutaiHSF2.
DMDMi462334.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 536536Heat shock factor protein 2PRO_0000124569Add
BLAST

Proteomic databases

MaxQBiQ03933.
PaxDbiQ03933.
PRIDEiQ03933.

PTM databases

PhosphoSiteiQ03933.

Expressioni

Gene expression databases

BgeeiQ03933.
CleanExiHS_HSF2.
ExpressionAtlasiQ03933. baseline and differential.
GenevestigatoriQ03933.

Organism-specific databases

HPAiCAB016067.
HPA031455.

Interactioni

Subunit structurei

DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
MTUS2Q5JR593EBI-10223348,EBI-742948
NUP62P371983EBI-2556750,EBI-347978
TPM3P067533EBI-2556750,EBI-355607
TPM3Q5VU623EBI-2556750,EBI-10184033
WDR5P619643EBI-2556750,EBI-540834

Protein-protein interaction databases

BioGridi109531. 21 interactions.
IntActiQ03933. 44 interactions.
STRINGi9606.ENSP00000357440.

Structurei

3D structure databases

ProteinModelPortaliQ03933.
SMRiQ03933. Positions 1-113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 19274Hydrophobic repeat HR-A/BAdd
BLAST
Regioni360 – 38526Hydrophobic repeat HR-CAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi108 – 12215Nuclear localization signalSequence AnalysisAdd
BLAST
Motifi195 – 21016Nuclear localization signalSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi170 – 1734Poly-Gln

Sequence similaritiesi

Belongs to the HSF family.Curated

Phylogenomic databases

eggNOGiCOG5169.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiQ03933.
KOiK09415.
OMAiPKTDKQL.
OrthoDBiEOG7353WM.
PhylomeDBiQ03933.
TreeFamiTF330401.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027712. HSF2.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR010542. Vert_HSTF_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF144. PTHR10015:SF144. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF06546. Vert_HS_TF. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q03933-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP
60 70 80 90 100
KYFKHNNMAS FVRQLNMYGF RKVVHIDSGI VKQERDGPVE FQHPYFKQGQ
110 120 130 140 150
DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ ETIESRLSEL
160 170 180 190 200
KSENESLWKE VSELRAKHAQ QQQVIRKIVQ FIVTLVQNNQ LVSLKRKRPL
210 220 230 240 250
LLNTNGAQKK NLFQHIVKEP TDNHHHKVPH SRTEGLKPRE RISDDIIIYD
260 270 280 290 300
VTDDNADEEN IPVIPETNED VISDPSNCSQ YPDIVIVEDD NEDEYAPVIQ
310 320 330 340 350
SGEQNEPARE SLSSGSDGSS PLMSSAVQLN GSSSLTSEDP VTMMDSILND
360 370 380 390 400
NINLLGKVEL LDYLDSIDCS LEDFQAMLSG RQFSIDPDLL VDLFTSSVQM
410 420 430 440 450
NPTDYINNTK SENKGLETTK NNVVQPVSEE GRKSKSKPDK QLIQYTAFPL
460 470 480 490 500
LAFLDGNPAS SVEQASTTAS SEVLSSVDKP IEVDELLDSS LDPEPTQSKL
510 520 530
VRLEPLTEAE ASEATLFYLC ELAPAPLDSD MPLLDS
Length:536
Mass (Da):60,348
Last modified:February 1, 1994 - v1
Checksum:i00DFD05CFD9DF0D3
GO
Isoform 2 (identifier: Q03933-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     393-410: Missing.

Show »
Length:518
Mass (Da):58,293
Checksum:i6682B51448E116BC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei393 – 41018Missing in isoform 2. 2 PublicationsVSP_041128Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65217 mRNA. Translation: AAA36017.1.
DQ492684 Genomic DNA. Translation: ABF47087.1.
AK294624 mRNA. Translation: BAG57805.1.
AK316377 mRNA. Translation: BAH14748.1.
AL121954, Z99129 Genomic DNA. Translation: CAC14262.2.
Z99129, AL121954 Genomic DNA. Translation: CAI20560.1.
CH471051 Genomic DNA. Translation: EAW48176.1.
BC112323 mRNA. Translation: AAI12324.1.
BC121050 mRNA. Translation: AAI21051.1.
BC121051 mRNA. Translation: AAI21052.1.
BC128420 mRNA. Translation: AAI28421.1.
CCDSiCCDS47470.1. [Q03933-2]
CCDS5124.1. [Q03933-1]
PIRiA41138.
RefSeqiNP_001129036.1. NM_001135564.1. [Q03933-2]
NP_004497.1. NM_004506.3. [Q03933-1]
UniGeneiHs.158195.

Genome annotation databases

EnsembliENST00000368455; ENSP00000357440; ENSG00000025156. [Q03933-1]
ENST00000452194; ENSP00000400380; ENSG00000025156. [Q03933-2]
GeneIDi3298.
KEGGihsa:3298.
UCSCiuc003pyu.2. human. [Q03933-1]
uc003pyv.2. human. [Q03933-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65217 mRNA. Translation: AAA36017.1.
DQ492684 Genomic DNA. Translation: ABF47087.1.
AK294624 mRNA. Translation: BAG57805.1.
AK316377 mRNA. Translation: BAH14748.1.
AL121954, Z99129 Genomic DNA. Translation: CAC14262.2.
Z99129, AL121954 Genomic DNA. Translation: CAI20560.1.
CH471051 Genomic DNA. Translation: EAW48176.1.
BC112323 mRNA. Translation: AAI12324.1.
BC121050 mRNA. Translation: AAI21051.1.
BC121051 mRNA. Translation: AAI21052.1.
BC128420 mRNA. Translation: AAI28421.1.
CCDSiCCDS47470.1. [Q03933-2]
CCDS5124.1. [Q03933-1]
PIRiA41138.
RefSeqiNP_001129036.1. NM_001135564.1. [Q03933-2]
NP_004497.1. NM_004506.3. [Q03933-1]
UniGeneiHs.158195.

3D structure databases

ProteinModelPortaliQ03933.
SMRiQ03933. Positions 1-113.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109531. 21 interactions.
IntActiQ03933. 44 interactions.
STRINGi9606.ENSP00000357440.

PTM databases

PhosphoSiteiQ03933.

Polymorphism and mutation databases

BioMutaiHSF2.
DMDMi462334.

Proteomic databases

MaxQBiQ03933.
PaxDbiQ03933.
PRIDEiQ03933.

Protocols and materials databases

DNASUi3298.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368455; ENSP00000357440; ENSG00000025156. [Q03933-1]
ENST00000452194; ENSP00000400380; ENSG00000025156. [Q03933-2]
GeneIDi3298.
KEGGihsa:3298.
UCSCiuc003pyu.2. human. [Q03933-1]
uc003pyv.2. human. [Q03933-2]

Organism-specific databases

CTDi3298.
GeneCardsiGC06P122762.
HGNCiHGNC:5225. HSF2.
HPAiCAB016067.
HPA031455.
MIMi140581. gene.
neXtProtiNX_Q03933.
PharmGKBiPA29494.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5169.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiQ03933.
KOiK09415.
OMAiPKTDKQL.
OrthoDBiEOG7353WM.
PhylomeDBiQ03933.
TreeFamiTF330401.

Enzyme and pathway databases

SignaLinkiQ03933.

Miscellaneous databases

GeneWikiiHSF2.
GenomeRNAii3298.
NextBioi13083.
PROiQ03933.
SOURCEiSearch...

Gene expression databases

BgeeiQ03933.
CleanExiHS_HSF2.
ExpressionAtlasiQ03933. baseline and differential.
GenevestigatoriQ03933.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027712. HSF2.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR010542. Vert_HSTF_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF144. PTHR10015:SF144. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF06546. Vert_HS_TF. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans."
    Schuetz T.J., Gallo G.J., Sheldon L., Tempst P., Kingston R.E.
    Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 65-71.
  2. NIEHS SNPs program
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
  7. "Hydrophobic coiled-coil domains regulate the subcellular localization of human heat shock factor 2."
    Sheldon L., Kingston R.E.
    Genes Dev. 7:1549-1558(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS.

Entry informationi

Entry nameiHSF2_HUMAN
AccessioniPrimary (citable) accession number: Q03933
Secondary accession number(s): B4DGJ4
, Q0VAH9, Q2M1K4, Q9H445
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 27, 2015
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.