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Protein

Zinc finger protein 85

Gene

ZNF85

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be a transcriptional repressor.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri146 – 16823C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri174 – 19623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri202 – 22423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri230 – 25223C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri258 – 28023C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri286 – 30823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri314 – 33623C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri342 – 36423C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri370 – 39223C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri398 – 42023C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri426 – 44823C2H2-type 11; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri454 – 47623C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri482 – 50423C2H2-type 13PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri510 – 53223C2H2-type 14PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri538 – 56023C2H2-type 15PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri566 – 58823C2H2-type 16PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: ProtInc
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: GO_Central
  • transcription corepressor activity Source: ProtInc
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 85
Alternative name(s):
Zinc finger protein HPF4
Zinc finger protein HTF1
Gene namesi
Name:ZNF85
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:13160. ZNF85.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37733.

Polymorphism and mutation databases

BioMutaiZNF85.
DMDMi215274175.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595Zinc finger protein 85PRO_0000047398Add
BLAST

Proteomic databases

EPDiQ03923.
PaxDbiQ03923.
PeptideAtlasiQ03923.
PRIDEiQ03923.

PTM databases

iPTMnetiQ03923.
PhosphoSiteiQ03923.

Expressioni

Tissue specificityi

Widely expressed, including in testis.2 Publications

Gene expression databases

BgeeiQ03923.
CleanExiHS_ZNF85.
ExpressionAtlasiQ03923. baseline and differential.
GenevisibleiQ03923. HS.

Organism-specific databases

HPAiHPA044760.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CEP76Q8TAP63EBI-10223330,EBI-742887

Protein-protein interaction databases

BioGridi113455. 2 interactions.
IntActiQ03923. 1 interaction.
STRINGi9606.ENSP00000329793.

Structurei

3D structure databases

ProteinModelPortaliQ03923.
SMRiQ03923. Positions 158-592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 7572KRABPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 16 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri146 – 16823C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri174 – 19623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri202 – 22423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri230 – 25223C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri258 – 28023C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri286 – 30823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri314 – 33623C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri342 – 36423C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri370 – 39223C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri398 – 42023C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri426 – 44823C2H2-type 11; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri454 – 47623C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri482 – 50423C2H2-type 13PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri510 – 53223C2H2-type 14PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri538 – 56023C2H2-type 15PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri566 – 58823C2H2-type 16PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00790000122969.
HOGENOMiHOG000234617.
HOVERGENiHBG018163.
InParanoidiQ03923.
KOiK09228.
OMAiKSFRMIS.
PhylomeDBiQ03923.
TreeFamiTF342117.

Family and domain databases

Gene3Di3.30.160.60. 16 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 7 hits.
PF13912. zf-C2H2_6. 3 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 15 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 15 hits.
PS50157. ZINC_FINGER_C2H2_2. 15 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q03923-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPLTFRDVA IEFSLKEWQC LDTAQRNLYR NVMLENYRNL VFLGITVSKP
60 70 80 90 100
DLITCLEQGK EAWSMKRHEI MVAKPTVMCS HFAQDLWPEQ NIKDSFQKVT
110 120 130 140 150
LKRYGKCRHE NLPLRKGCES MDECKMHKGG CNGLNQCLTA TQSKIFQCDK
160 170 180 190 200
YVKVAHKFSN SNRHEIRHTK KKPFKCTKCG KSFGMISCLT EHSRIHTRVN
210 220 230 240 250
FYKCEECGKA FNWSSTLTKH KRIHTGEKPY KCEECGKAFN QSSNLIKHKK
260 270 280 290 300
IHTGEKPYKC EECGKTFNRF STLTTHKIIH TGEKPYKCKE CGKAFNRSST
310 320 330 340 350
LTTHRKIHTG EKPYKCEECG KAFKQSSNLT THKIIHTGEK PYKCKKCGKA
360 370 380 390 400
FNQSAHLTTH EVIHTGEKPY KCEKCGKAFN HFSHLTTHKI IHTGEKPYKC
410 420 430 440 450
KECGKAFKHS STLTKHKIIH TGEKPYKCKE CEKAFNQSSK LTEHKKIHTG
460 470 480 490 500
EKPYECEKCG KAFNQSSNLT RHKKSHTEEK PYKCEECGKG FKWPSTLTIH
510 520 530 540 550
KIIHTGEKPY KCEECGKAFN QSSKLTKHKK IHTGEKPYTC EECGKAFNQS
560 570 580 590
SNLTKHKRIH TGEKPYKCEE CDKAFKWSSV LTKHKIIHTG EKLQI
Length:595
Mass (Da):68,736
Last modified:November 25, 2008 - v3
Checksum:iF21FA8927C28DE90
GO
Isoform 2 (identifier: Q03923-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-76: Missing.

Note: No experimental confirmation available.
Show »
Length:562
Mass (Da):65,056
Checksum:i8CC193C2680A8A11
GO
Isoform 3 (identifier: Q03923-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-87: VMCSHFAQDLW → ESYSVTQSGMQ
     88-595: Missing.

Show »
Length:87
Mass (Da):10,088
Checksum:i254588D03599BA03
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti428 – 4281C → S in AAA79179 (PubMed:9839802).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti60 – 601K → T.
Corresponds to variant rs7254311 [ dbSNP | Ensembl ].
VAR_033552
Natural varianti84 – 841Q → R.2 Publications
Corresponds to variant rs56321708 [ dbSNP | Ensembl ].
VAR_061932
Natural varianti115 – 1151R → I.1 Publication
Corresponds to variant rs56231962 [ dbSNP | Ensembl ].
VAR_061933
Natural varianti177 – 1771T → R.1 Publication
Corresponds to variant rs56393308 [ dbSNP | Ensembl ].
VAR_061934
Natural varianti184 – 1841G → R.1 Publication
Corresponds to variant rs11665978 [ dbSNP | Ensembl ].
VAR_033553
Natural varianti266 – 2661T → A.1 Publication
Corresponds to variant rs1063156 [ dbSNP | Ensembl ].
VAR_047515
Natural varianti270 – 2701F → S.
Corresponds to variant rs11670246 [ dbSNP | Ensembl ].
VAR_033554

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei44 – 7633Missing in isoform 2. 1 PublicationVSP_035771Add
BLAST
Alternative sequencei77 – 8711VMCSHFAQDLW → ESYSVTQSGMQ in isoform 3. 1 PublicationVSP_042154Add
BLAST
Alternative sequencei88 – 595508Missing in isoform 3. 1 PublicationVSP_042155Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35376 mRNA. Translation: AAA79179.1.
AC008739 Genomic DNA. No translation available.
BC068066 mRNA. Translation: AAH68066.1.
M61866 mRNA. Translation: AAA52689.1.
M61868 mRNA. Translation: AAA58671.1.
CCDSiCCDS32977.1. [Q03923-1]
CCDS58657.1. [Q03923-3]
PIRiA39384.
C39384.
G02075.
RefSeqiNP_001243101.1. NM_001256172.1. [Q03923-3]
NP_003420.2. NM_003429.4. [Q03923-1]
UniGeneiHs.37138.

Genome annotation databases

EnsembliENST00000300540; ENSP00000300540; ENSG00000105750. [Q03923-3]
ENST00000328178; ENSP00000329793; ENSG00000105750. [Q03923-1]
ENST00000345030; ENSP00000342340; ENSG00000105750. [Q03923-2]
ENST00000613159; ENSP00000479316; ENSG00000278091. [Q03923-2]
ENST00000615320; ENSP00000483118; ENSG00000278091. [Q03923-1]
ENST00000615882; ENSP00000479120; ENSG00000278091. [Q03923-3]
GeneIDi7639.
KEGGihsa:7639.
UCSCiuc002npf.5. human. [Q03923-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35376 mRNA. Translation: AAA79179.1.
AC008739 Genomic DNA. No translation available.
BC068066 mRNA. Translation: AAH68066.1.
M61866 mRNA. Translation: AAA52689.1.
M61868 mRNA. Translation: AAA58671.1.
CCDSiCCDS32977.1. [Q03923-1]
CCDS58657.1. [Q03923-3]
PIRiA39384.
C39384.
G02075.
RefSeqiNP_001243101.1. NM_001256172.1. [Q03923-3]
NP_003420.2. NM_003429.4. [Q03923-1]
UniGeneiHs.37138.

3D structure databases

ProteinModelPortaliQ03923.
SMRiQ03923. Positions 158-592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113455. 2 interactions.
IntActiQ03923. 1 interaction.
STRINGi9606.ENSP00000329793.

PTM databases

iPTMnetiQ03923.
PhosphoSiteiQ03923.

Polymorphism and mutation databases

BioMutaiZNF85.
DMDMi215274175.

Proteomic databases

EPDiQ03923.
PaxDbiQ03923.
PeptideAtlasiQ03923.
PRIDEiQ03923.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300540; ENSP00000300540; ENSG00000105750. [Q03923-3]
ENST00000328178; ENSP00000329793; ENSG00000105750. [Q03923-1]
ENST00000345030; ENSP00000342340; ENSG00000105750. [Q03923-2]
ENST00000613159; ENSP00000479316; ENSG00000278091. [Q03923-2]
ENST00000615320; ENSP00000483118; ENSG00000278091. [Q03923-1]
ENST00000615882; ENSP00000479120; ENSG00000278091. [Q03923-3]
GeneIDi7639.
KEGGihsa:7639.
UCSCiuc002npf.5. human. [Q03923-1]

Organism-specific databases

CTDi7639.
GeneCardsiZNF85.
HGNCiHGNC:13160. ZNF85.
HPAiHPA044760.
MIMi603899. gene.
neXtProtiNX_Q03923.
PharmGKBiPA37733.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00790000122969.
HOGENOMiHOG000234617.
HOVERGENiHBG018163.
InParanoidiQ03923.
KOiK09228.
OMAiKSFRMIS.
PhylomeDBiQ03923.
TreeFamiTF342117.

Miscellaneous databases

GenomeRNAii7639.
PROiQ03923.
SOURCEiSearch...

Gene expression databases

BgeeiQ03923.
CleanExiHS_ZNF85.
ExpressionAtlasiQ03923. baseline and differential.
GenevisibleiQ03923. HS.

Family and domain databases

Gene3Di3.30.160.60. 16 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 7 hits.
PF13912. zf-C2H2_6. 3 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 15 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 15 hits.
PS50157. ZINC_FINGER_C2H2_2. 15 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Functional analysis of ZNF85 KRAB zinc finger protein, a member of the highly homologous ZNF91 family."
    Poncelet D.A., Bellefroid E.J., Bastiaens P.V., Demoitie M.A., Marine J.C., Pendeville H., Alami Y., Devos N., Lecocq P.J., Ogawa T., Muller M., Martial J.A.
    DNA Cell Biol. 17:931-943(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANTS ARG-84 AND ALA-266.
    Tissue: Placenta.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Lung.
  4. "The evolutionarily conserved Kruppel-associated box domain defines a subfamily of eukaryotic multifingered proteins."
    Bellefroid E.J., Poncelet D.A., Lecocq P.J., Revelant O., Martial J.A.
    Proc. Natl. Acad. Sci. U.S.A. 88:3608-3612(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-196 (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 12-196 (ISOFORM 2), VARIANTS ARG-84; ILE-115; ARG-177 AND ARG-184, TISSUE SPECIFICITY.

Entry informationi

Entry nameiZNF85_HUMAN
AccessioniPrimary (citable) accession number: Q03923
Secondary accession number(s): B9ZVP4, Q6NVI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 25, 2008
Last modified: July 6, 2016
This is version 150 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.