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Protein

NEDD8-like protein RUB1

Gene

RUB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein modifier that can be covalently attached to lysine residues of target proteins. Activated by the dimeric UBA3-ULA1 E1 enzyme and conjugated by the E2 UBC12 to substrate proteins. RUB1-conjugated (neddylated) substrate proteins include the cullins CDC53, RTT101 and CUL3, and the modification enhances the ubiquitin-ligase activity of the corresponding cullin-RING-based E3 ubiquitin-protein ligase complexes (CRLs).3 Publications

GO - Molecular functioni

  • protein tag Source: SGD

GO - Biological processi

  • protein neddylation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-29736-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-like protein RUB1
Alternative name(s):
Related to ubiquitin protein 1
Ubiquitin-like protein RUB1
Gene namesi
Name:RUB1
Ordered Locus Names:YDR139C
ORF Names:YD9302.15C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR139C.
SGDiS000002546. RUB1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000359791 – 76NEDD8-like protein RUB1Add BLAST76
PropeptideiPRO_000003598077Curated1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond

Proteomic databases

MaxQBiQ03919.
PRIDEiQ03919.

Interactioni

Subunit structurei

Interacts with CDC53 and DCN1.2 Publications

Protein-protein interaction databases

BioGridi32193. 46 interactors.
DIPiDIP-1627N.
IntActiQ03919. 8 interactors.
MINTiMINT-407484.

Structurei

3D structure databases

ProteinModelPortaliQ03919.
SMRiQ03919.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 74Ubiquitin-likePROSITE-ProRule annotationAdd BLAST74

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233942.
InParanoidiQ03919.
KOiK12158.
OMAiCEESDKI.
OrthoDBiEOG092C5P9Y.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03919-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVKVKTLTG KEISVELKES DLVYHIKELL EEKEGIPPSQ QRLIFQGKQI
60 70
DDKLTVTDAH LVEGMQLHLV LTLRGGN
Length:77
Mass (Da):8,693
Last modified:November 1, 1996 - v1
Checksum:iF05DBF3C8E464FD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16890 Genomic DNA. Translation: CAA76516.1.
Z48179 Genomic DNA. Translation: CAA88221.1.
BK006938 Genomic DNA. Translation: DAA11982.1.
PIRiS51867.
RefSeqiNP_010423.4. NM_001180446.3.

Genome annotation databases

EnsemblFungiiYDR139C; YDR139C; YDR139C.
GeneIDi851717.
KEGGisce:YDR139C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16890 Genomic DNA. Translation: CAA76516.1.
Z48179 Genomic DNA. Translation: CAA88221.1.
BK006938 Genomic DNA. Translation: DAA11982.1.
PIRiS51867.
RefSeqiNP_010423.4. NM_001180446.3.

3D structure databases

ProteinModelPortaliQ03919.
SMRiQ03919.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32193. 46 interactors.
DIPiDIP-1627N.
IntActiQ03919. 8 interactors.
MINTiMINT-407484.

Proteomic databases

MaxQBiQ03919.
PRIDEiQ03919.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR139C; YDR139C; YDR139C.
GeneIDi851717.
KEGGisce:YDR139C.

Organism-specific databases

EuPathDBiFungiDB:YDR139C.
SGDiS000002546. RUB1.

Phylogenomic databases

HOGENOMiHOG000233942.
InParanoidiQ03919.
KOiK12158.
OMAiCEESDKI.
OrthoDBiEOG092C5P9Y.

Enzyme and pathway databases

BioCyciYEAST:G3O-29736-MONOMER.

Miscellaneous databases

PROiQ03919.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRUB1_YEAST
AccessioniPrimary (citable) accession number: Q03919
Secondary accession number(s): D6VSC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1310 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.