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Q03909

- HEMA_I89A7

UniProt

Q03909 - HEMA_I89A7

Protein

Hemagglutinin

Gene

HA

Organism
Influenza A virus (strain A/Equine/Jillin/1/1989 H3N8)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei350 – 3512Cleavage; by hostBy similarity

    GO - Biological processi

    1. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
    2. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    3. fusion of virus membrane with host plasma membrane Source: InterPro
    4. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin

    Keywords - Biological processi

    Clathrin- and caveolin-independent endocytosis of virus by host, Clathrin-mediated endocytosis of virus by host, Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin
    Cleaved into the following 2 chains:
    Gene namesi
    Name:HA
    OrganismiInfluenza A virus (strain A/Equine/Jillin/1/1989 H3N8)
    Taxonomic identifieri385585 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Equus caballus (Horse) [TaxID: 9796]

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host apical cell membrane; Single-pass type I membrane protein
    Note: Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 349328Hemagglutinin HA1 chainPRO_0000280189Add
    BLAST
    Chaini351 – 571221Hemagglutinin HA2 chainPRO_0000280190Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi29 – 291N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi35 ↔ 487Interchain (between HA1 and HA2 chains)By similarity
    Glycosylationi43 – 431N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi59 – 591N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi73 ↔ 298By similarity
    Glycosylationi84 – 841N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi85 ↔ 97By similarity
    Disulfide bondi118 ↔ 160By similarity
    Glycosylationi186 – 1861N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi302 ↔ 326By similarity
    Glycosylationi306 – 3061N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi494 ↔ 498By similarity
    Glycosylationi504 – 5041N-linked (GlcNAc...); by hostSequence Analysis
    Lipidationi560 – 5601S-palmitoyl cysteine; by hostBy similarity
    Lipidationi567 – 5671S-palmitoyl cysteine; by hostBy similarity
    Lipidationi570 – 5701S-palmitoyl cysteine; by hostBy similarity

    Post-translational modificationi

    In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by Clara cells By similarity.By similarity
    Palmitoylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Interactioni

    Subunit structurei

    Homotrimer of disulfide-linked HA1-HA2.By similarity

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HXYX-ray2.60C327-339[»]
    2IPKX-ray2.30C327-339[»]
    2XN9X-ray2.30F327-339[»]
    ProteinModelPortaliQ03909.
    SMRiQ03909. Positions 30-523.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 535512ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini557 – 57115CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei536 – 55621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di2.10.77.10. 1 hit.
    3.90.20.10. 1 hit.
    3.90.209.20. 1 hit.
    InterProiIPR008980. Capsid_hemagglutn.
    IPR013828. Hemagglutn_HA1_a/b_dom.
    IPR013827. Hemagglutn_HA1_b-rbn_dom.
    IPR000149. Hemagglutn_influenz_A.
    IPR001364. Hemagglutn_influenz_A/B.
    IPR013829. Hemagglutn_stalk.
    [Graphical view]
    PfamiPF00509. Hemagglutinin. 1 hit.
    [Graphical view]
    PRINTSiPR00330. HEMAGGLUTN1.
    PR00329. HEMAGGLUTN12.
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03909-1 [UniParc]FASTAAdd to Basket

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    MLSLIMRTVI ALSYIFCLAF GQGLPWNDNN TATLCLGHHA VPNGTIVKTI    50
    TDDQIEVTNA TELVQSSSTG KICNNPHRIL DGGNCTLIDA LLGDPHCNVF 100
    QYETWDLFVE RTNAFSNCYP YDVPDYASLR SIVASSGTLE FFAESFTWTG 150
    VTQNGGSSAC KRGTASSFFS RLNWLTKSGN AYPLLNVTMP NNDNFDKLYI 200
    WGVHHPSTNQ EQTELYVQAS GRVTVSTRKS QQTVIPNIGS RPWVRGQSGR 250
    VSIYWTIVKP GDVLVINSNG NLIAPRGYFK VRTGKSSIMR SDAPIDTCIS 300
    ECITPNGSIP NDKPFQNVNK ITYGACPKYV KQNTLKLATG MRNVPEKQIR 350
    GIFGAIAGFI ENGWEGMIDG WYGFRHQNSE GTGQAADLKS TQAALDQING 400
    KLNRVIEKTN EKFHQIEKEF SEVEGRIQDL EKYVEDTKID LWSYNAELLV 450
    ALENQHTIDL TDSEMNKLFE KTRRQLRENA EDMGNGCFKI YHNCDNACIE 500
    SIRNGTYDHN IYRDEALNNR FQIKGVELKS GYKDWILWIS FAISCFLLCV 550
    VLLGFIMWAC QKGNIRCNIC F 571
    Length:571
    Mass (Da):64,105
    Last modified:November 1, 1996 - v1
    Checksum:i718DAA0F291CE349
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M65018 Genomic RNA. Translation: AAA43151.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M65018 Genomic RNA. Translation: AAA43151.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HXY X-ray 2.60 C 327-339 [» ]
    2IPK X-ray 2.30 C 327-339 [» ]
    2XN9 X-ray 2.30 F 327-339 [» ]
    ProteinModelPortali Q03909.
    SMRi Q03909. Positions 30-523.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.10.77.10. 1 hit.
    3.90.20.10. 1 hit.
    3.90.209.20. 1 hit.
    InterProi IPR008980. Capsid_hemagglutn.
    IPR013828. Hemagglutn_HA1_a/b_dom.
    IPR013827. Hemagglutn_HA1_b-rbn_dom.
    IPR000149. Hemagglutn_influenz_A.
    IPR001364. Hemagglutn_influenz_A/B.
    IPR013829. Hemagglutn_stalk.
    [Graphical view ]
    Pfami PF00509. Hemagglutinin. 1 hit.
    [Graphical view ]
    PRINTSi PR00330. HEMAGGLUTN1.
    PR00329. HEMAGGLUTN12.
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Emergence of a new influenza A virus in horses from avian sources."
      Guo Y., Wang M.G., Kawaoka Y., Gorman O.T., Ito T., Webster R.G.
      Submitted (JUN-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiHEMA_I89A7
    AccessioniPrimary (citable) accession number: Q03909
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2007
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Major glycoprotein, comprises over 80% of the envelope proteins present in virus particle.
    The extent of infection into host organism is determined by HA. Influenza viruses bud from the apical surface of polarized epithelial cells (e.g. bronchial epithelial cells) into lumen of lungs and are therefore usually pneumotropic. The reason is that HA is cleaved by tryptase clara which is restricted to lungs. However, HAs of H5 and H7 pantropic avian viruses subtypes can be cleaved by furin and subtilisin-type enzymes, allowing the virus to grow in other organs than lungs.
    The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3