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Q03909

- HEMA_I89A7

UniProt

Q03909 - HEMA_I89A7

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Protein
Hemagglutinin
Gene
HA
Organism
Influenza A virus (strain A/Equine/Jillin/1/1989 H3N8)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei350 – 3512Cleavage; by host By similarity

GO - Biological processi

  1. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
  2. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  3. fusion of virus membrane with host plasma membrane Source: InterPro
  4. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Clathrin- and caveolin-independent endocytosis of virus by host, Clathrin-mediated endocytosis of virus by host, Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin
Cleaved into the following 2 chains:
Gene namesi
Name:HA
OrganismiInfluenza A virus (strain A/Equine/Jillin/1/1989 H3N8)
Taxonomic identifieri385585 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Equus caballus (Horse) [TaxID: 9796]

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host apical cell membrane; Single-pass type I membrane protein
Note: Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 535512Extracellular Reviewed prediction
Add
BLAST
Transmembranei536 – 55621Helical; Reviewed prediction
Add
BLAST
Topological domaini557 – 57115Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 349328Hemagglutinin HA1 chain
PRO_0000280189Add
BLAST
Chaini351 – 571221Hemagglutinin HA2 chain
PRO_0000280190Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi35 ↔ 487Interchain (between HA1 and HA2 chains) By similarity
Glycosylationi43 – 431N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi59 – 591N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi73 ↔ 298 By similarity
Glycosylationi84 – 841N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi85 ↔ 97 By similarity
Disulfide bondi118 ↔ 160 By similarity
Glycosylationi186 – 1861N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi302 ↔ 326 By similarity
Glycosylationi306 – 3061N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi494 ↔ 498 By similarity
Glycosylationi504 – 5041N-linked (GlcNAc...); by host Reviewed prediction
Lipidationi560 – 5601S-palmitoyl cysteine; by host By similarity
Lipidationi567 – 5671S-palmitoyl cysteine; by host By similarity
Lipidationi570 – 5701S-palmitoyl cysteine; by host By similarity

Post-translational modificationi

In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by Clara cells By similarity.
Palmitoylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HA1-HA2 By similarity.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HXYX-ray2.60C327-339[»]
2IPKX-ray2.30C327-339[»]
2XN9X-ray2.30F327-339[»]
ProteinModelPortaliQ03909.
SMRiQ03909. Positions 30-523.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view]
PfamiPF00509. Hemagglutinin. 1 hit.
[Graphical view]
PRINTSiPR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03909-1 [UniParc]FASTAAdd to Basket

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MLSLIMRTVI ALSYIFCLAF GQGLPWNDNN TATLCLGHHA VPNGTIVKTI    50
TDDQIEVTNA TELVQSSSTG KICNNPHRIL DGGNCTLIDA LLGDPHCNVF 100
QYETWDLFVE RTNAFSNCYP YDVPDYASLR SIVASSGTLE FFAESFTWTG 150
VTQNGGSSAC KRGTASSFFS RLNWLTKSGN AYPLLNVTMP NNDNFDKLYI 200
WGVHHPSTNQ EQTELYVQAS GRVTVSTRKS QQTVIPNIGS RPWVRGQSGR 250
VSIYWTIVKP GDVLVINSNG NLIAPRGYFK VRTGKSSIMR SDAPIDTCIS 300
ECITPNGSIP NDKPFQNVNK ITYGACPKYV KQNTLKLATG MRNVPEKQIR 350
GIFGAIAGFI ENGWEGMIDG WYGFRHQNSE GTGQAADLKS TQAALDQING 400
KLNRVIEKTN EKFHQIEKEF SEVEGRIQDL EKYVEDTKID LWSYNAELLV 450
ALENQHTIDL TDSEMNKLFE KTRRQLRENA EDMGNGCFKI YHNCDNACIE 500
SIRNGTYDHN IYRDEALNNR FQIKGVELKS GYKDWILWIS FAISCFLLCV 550
VLLGFIMWAC QKGNIRCNIC F 571
Length:571
Mass (Da):64,105
Last modified:November 1, 1996 - v1
Checksum:i718DAA0F291CE349
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M65018 Genomic RNA. Translation: AAA43151.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M65018 Genomic RNA. Translation: AAA43151.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HXY X-ray 2.60 C 327-339 [» ]
2IPK X-ray 2.30 C 327-339 [» ]
2XN9 X-ray 2.30 F 327-339 [» ]
ProteinModelPortali Q03909.
SMRi Q03909. Positions 30-523.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProi IPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view ]
Pfami PF00509. Hemagglutinin. 1 hit.
[Graphical view ]
PRINTSi PR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Emergence of a new influenza A virus in horses from avian sources."
    Guo Y., Wang M.G., Kawaoka Y., Gorman O.T., Ito T., Webster R.G.
    Submitted (JUN-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_I89A7
AccessioniPrimary (citable) accession number: Q03909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Major glycoprotein, comprises over 80% of the envelope proteins present in virus particle.
The extent of infection into host organism is determined by HA. Influenza viruses bud from the apical surface of polarized epithelial cells (e.g. bronchial epithelial cells) into lumen of lungs and are therefore usually pneumotropic. The reason is that HA is cleaved by tryptase clara which is restricted to lungs. However, HAs of H5 and H7 pantropic avian viruses subtypes can be cleaved by furin and subtilisin-type enzymes, allowing the virus to grow in other organs than lungs.
The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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