Reviewed, UniProtKB/Swiss-Prot Q03899 (YD131_YEAST)

Names and origin

Protein names

Recommended name:
    F-box protein YDR131C

Gene names

Ordered Locus Names:

YDR131C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1. Component of the probable SCF(YDR131C) complex containing CDC53, SKP1, RBX1 and YDR131C. Ref.4
Subcellular location	Vacuole. Ref.3
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Vacuole
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	protein ubiquitination during ubiquitin-dependent protein catabolic process Ref.2 Inferred from genetic interaction. Source: SGD
Cellular component	SCF ubiquitin ligase complex Ref.2 Inferred from physical interaction. Source: SGD vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RIX1	P38883	1	EBI-36201,EBI-24899
SKP1	P52286	1	EBI-36201,EBI-4090
YRB2	P40517	1	EBI-36201,EBI-29534

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	F-box protein YDR131C		PRO_0000253804
Regions
	Domain	1 – 44	44	F-box

Sequences

	Sequence		Length	Mass (Da)
	Q03899-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7004D9665DAE2CEF	FASTA	556	65,243
	10 20 30 40 50 60 MFDKLPYEIF KQIAWRIPQE DKISLTYVCK RSYESIIPFI YQNLFLNETY HINGDYDNSF 70 80 90 100 110 120 GTCYWSVLNF HYIDEDDSNT KNDMSNRRLA KVKFSYFERT LAESPKRLCP LINRIRCTWH 130 140 150 160 170 180 LNEDVMTNVL KLLSEYGSNL KFVDQFVRSS VNKGLEPLSK QLKTLTLTPP TLMPTHNSVS 190 200 210 220 230 240 GSYLNKIDRL LLKCDLSRLE KLSIHINALK YFKNTGSPMK IKALVLNLRP DTLNLAEYDA 250 260 270 280 290 300 SDDFLKELEY IDIFDASTLR QLEILSWYSR DDFPSGEEGG FDRLYVKWGL EGFWKFPNIE 310 320 330 340 350 360 KLSLASLVYS EFFLMNCLAV FHNLKILKLD YMGKFDFDVS LINFLSKQVC GKKLQRFDIH 370 380 390 400 410 420 CQLNHRLFFP MTDNPLTRLN FDGFCPCSTC KNTIHEVILK KIFPETRSKL LKNPNKFQAH 430 440 450 460 470 480 NFFYQMFFEN KIMPYTNIID NESPAMGWDS VPIETFVRKF NENLQSTIEN TENITVNKIT 490 500 510 520 530 540 REDAISLYHL YLHYLKDVFK VFEQSLPNLE YLTINGIPTK IIQVDELQRC AVPLFYNNGY 550 KSNSVYELVD AEALFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis." Willems A.R., Goh T., Taylor L., Chernushevich I., Shevchenko A., Tyers M. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 354:1533-1550(1999) [PubMed: 10582239] [Abstract] Cited for: PROTEIN SEQUENCE OF 132-148 AND 521-529, IDENTIFICATION IN SCF COMPLEX, MASS SPECTROMETRY.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro." Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M. Proteins 54:455-467(2004) [PubMed: 14747994] [Abstract] Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(YDR131C) COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z48179 Genomic DNA. Translation: CAA88212.1.
PIR	S51858.
RefSeq	NP_010416.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:867N.
IntAct	Q03899. 5 interactions.
Proteomic databases
PeptideAtlas	Q03899.
PRIDE	Q03899.
Genome annotation databases
Ensembl	YDR131C. Saccharomyces cerevisiae. [Contig view]
GeneID	851709.
GenomeReviews	Gene locus YDR131C in contig Z71256_GR.
KEGG	sce:YDR131C.
NMPDR	fig|4932.3.peg.1165.
Organism-specific databases
CYGD	YDR131c.
SGD	S000002538. YDR131C.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q03899.
OMA	Q03899. YELVDAE.
Gene expression databases
GermOnline	YDR131C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	969398.

Entry information

Entry name

YD131_YEAST

Accession

Primary (citable) accession number: Q03899

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)