Q03880 (PKR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type ATPase assembly factor PKR1

Gene names

Name:	PKR1
Ordered Locus Names:	YMR123W
ORF Names:	YM8564.05

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions together with the other V-type ATPase assembly factors in the endoplasmic reticulum to efficiently assemble the V-type ATPase membrane sector V0. Ref.6
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4 Ref.6.
Miscellaneous	Present with 5750 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the PKR1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	vacuolar proton-transporting V-type ATPase complex assembly Inferred from mutant phenotype Ref.6. Source: SGD
Cellular component	integral to endoplasmic reticulum membrane Inferred from direct assay Ref.6. Source: SGD vacuole Inferred from mutant phenotype Ref.6. Source: GOC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 122	122	V-type ATPase assembly factor PKR1		PRO_0000203297
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Helical; Potential
	Topological domain	42 – 46	5	Lumenal Potential
	Transmembrane	47 – 67	21	Helical; Potential
	Topological domain	68 – 122	55	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q03880 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 5CAA6929D55F2C40	FASTA	122	13,962
	10 20 30 40 50 60 MANFFVRLWE SVFEPGTSPQ LIIATHVSFV ALLLTLIWLI YATNGNIHFY ALFCISLLLW 70 80 90 100 110 120 ITVIWFINEL SHVKLKDNDE LDKDANKKDD SAIKEDSEDK QESGKSTSTA RRTQAQSRSR KA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"PKR1 encodes an assembly factor for the yeast V-type ATPase." Davis-Kaplan S.R., Compton M.A., Flannery A.R., Ward D.M., Kaplan J., Stevens T.H., Graham L.A. J. Biol. Chem. 281:32025-32035(2006) [PubMed: 16926153] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49273 Genomic DNA. Translation: CAA89272.1. AY558408 Genomic DNA. Translation: AAS56734.1. BK006946 Genomic DNA. Translation: DAA10020.1.
PIR	S54492.
RefSeq	NP_013842.1. NM_001182624.1.
3D structure databases
ProteinModelPortal	Q03880.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4436N.
IntAct	Q03880. 19 interactions.
MINT	MINT-545112.
STRING	Q03880.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR123W; YMR123W; YMR123W.
GeneID	855153.
KEGG	sce:YMR123W.
NMPDR	fig|4932.3.peg.4891.
Organism-specific databases
CYGD	YMR123w.
SGD	S000004730. PKR1.
Phylogenomic databases
eggNOG	fuNOG11962.
GeneTree	EFGT00050000005256.
HOGENOM	HBG756741.
OMA	ATHASFI.
OrthoDB	EOG4K3Q62.
Gene expression databases
ArrayExpress	Q03880.
Genevestigator	Q03880.
GermOnline	YMR123W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013945. ER_Pkr1. [Graphical view]
Pfam	PF08636. Pkr1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978560.

Entry information

Entry name

PKR1_YEAST

Accession

Primary (citable) accession number: Q03880
Secondary accession number(s): D6VZU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program