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Protein

Monothiol glutaredoxin-3

Gene

GRX3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters (By similarity). Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit (Probable).By similarityCurated1 Publication

Miscellaneous

Present with 11000 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi176Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: SGD
  • cell redox homeostasis Source: InterPro
  • cellular iron ion homeostasis Source: SGD
  • cellular response to oxidative stress Source: SGD
  • negative regulation of transcription regulatory region DNA binding Source: SGD
  • positive regulation of histone deacetylation Source: SGD

Keywordsi

Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-100

Names & Taxonomyi

Protein namesi
Recommended name:
Monothiol glutaredoxin-3
Gene namesi
Name:GRX3
Ordered Locus Names:YDR098C
ORF Names:YD8557.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR098C
SGDiS000002505 GRX3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022491 – 250Monothiol glutaredoxin-3Add BLAST250

Proteomic databases

PaxDbiQ03835
PRIDEiQ03835

Interactioni

Subunit structurei

Homodimer. Heterodimer with FRA2.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi32153, 84 interactors
DIPiDIP-1350N
IntActiQ03835, 9 interactors
MINTiQ03835
STRINGi4932.YDR098C

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Helixi9 – 16Combined sources8
Turni17 – 22Combined sources6
Beta strandi25 – 30Combined sources6
Helixi37 – 49Combined sources13
Helixi51 – 53Combined sources3
Beta strandi56 – 62Combined sources7
Turni63 – 65Combined sources3
Helixi67 – 72Combined sources6
Beta strandi77 – 85Combined sources9
Beta strandi88 – 93Combined sources6
Helixi98 – 112Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D6IX-ray1.50A/B2-113[»]
ProteinModelPortaliQ03835
SMRiQ03835
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03835

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 110ThioredoxinPROSITE-ProRule annotationAdd BLAST110
Domaini151 – 250GlutaredoxinPROSITE-ProRule annotationAdd BLAST100

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

GeneTreeiENSGT00550000075030
HOGENOMiHOG000165751
InParanoidiQ03835
OrthoDBiEOG092C3TIP

Family and domain databases

CDDicd03028 GRX_PICOT_like, 1 hit
InterProiView protein in InterPro
IPR002109 Glutaredoxin
IPR033658 GRX_PICOT-like
IPR004480 Monothiol_GRX-rel
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PANTHERiPTHR10293 PTHR10293, 1 hit
PfamiView protein in Pfam
PF00462 Glutaredoxin, 1 hit
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 2 hits
PROSITEiView protein in PROSITE
PS51354 GLUTAREDOXIN_2, 1 hit
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q03835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVIEINDQE QFTYLTTTAA GDKLIVLYFH TSWAEPCKAL KQVFEAISNE
60 70 80 90 100
PSNSNVSFLS IDADENSEIS ELFEISAVPY FIIIHKGTIL KELSGADPKE
110 120 130 140 150
YVSLLEDCKN SVNSGSSQTH TMENANVNEG SHNDEDDDDE EEEEETEEQI
160 170 180 190 200
NARLTKLVNA APVMLFMKGS PSEPKCGFSR QLVGILREHQ VRFGFFDILR
210 220 230 240 250
DESVRQNLKK FSEWPTFPQL YINGEFQGGL DIIKESLEED PDFLQHALQS
Length:250
Mass (Da):28,261
Last modified:October 14, 2015 - v2
Checksum:i8CC1A04EC1E7DAB3
GO

Sequence cautioni

The sequence CAA87672 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47746 Genomic DNA Translation: CAA87672.1 Different initiation.
BK006938 Genomic DNA Translation: DAA11943.2
PIRiS51247
RefSeqiNP_010383.4, NM_001180406.4

Genome annotation databases

EnsemblFungiiYDR098C; YDR098C; YDR098C
GeneIDi851672
KEGGisce:YDR098C

Similar proteinsi

Entry informationi

Entry nameiGLRX3_YEAST
AccessioniPrimary (citable) accession number: Q03835
Secondary accession number(s): D6VS83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 14, 2015
Last modified: June 20, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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