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Protein

Transcriptional activator/repressor GIS1

Gene

GIS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in the regulation of gene expression upon nutrient starvation. Recognizes and binds to the post-diauxic-shift element 5'-T[AT]AGGGAT-3' in the promoter region. Can act as a transcriptional activator (e.g. of stress genes like SSA3, HSP12 and HSP26) as well as a repressor (e.g. of pyrophosphate phosphatase DPP1). GIS1 also acts as a DNA damage-responsive transcriptional repressor of photolyase PHR1.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri828 – 851C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri857 – 882C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

GO - Biological processi

  • chronological cell aging Source: SGD
  • histone demethylation Source: SGD
  • maintenance of stationary phase in response to starvation Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of phospholipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29699-MONOMER.
ReactomeiR-SCE-3214842. HDMs demethylate histones.
R-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator/repressor GIS1
Gene namesi
Name:GIS1
Ordered Locus Names:YDR096W
ORF Names:YD8557.01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR096W.
SGDiS000002503. GIS1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000468061 – 894Transcriptional activator/repressor GIS1Add BLAST894

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70PhosphoserineCombined sources1
Modified residuei343PhosphoserineCombined sources1
Modified residuei690PhosphoserineCombined sources1
Modified residuei694PhosphoserineCombined sources1
Modified residuei696PhosphoserineCombined sources1
Modified residuei734PhosphoserineCombined sources1
Modified residuei747PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03833.
PRIDEiQ03833.

PTM databases

iPTMnetiQ03833.

Expressioni

Inductioni

Induced upon nutrient starvation.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi32151. 83 interactors.
IntActiQ03833. 33 interactors.
MINTiMINT-4480789.

Structurei

3D structure databases

ProteinModelPortaliQ03833.
SMRiQ03833.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 53JmjNPROSITE-ProRule annotationAdd BLAST42
Domaini170 – 324JmjCPROSITE-ProRule annotationAdd BLAST155

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili90 – 110Sequence analysisAdd BLAST21
Coiled coili361 – 385Sequence analysisAdd BLAST25

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi316 – 332Bipartite nuclear localization signalAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi462 – 806Asn-richAdd BLAST345

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 JmjC domain.PROSITE-ProRule annotation
Contains 1 JmjN domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri828 – 851C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri857 – 882C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063342.
HOGENOMiHOG000112726.
KOiK19798.
OrthoDBiEOG092C2SZJ.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR003347. JmjC_dom.
IPR003349. JmjN.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02373. JmjC. 1 hit.
PF02375. JmjN. 1 hit.
[Graphical view]
SMARTiSM00558. JmjC. 1 hit.
SM00545. JmjN. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
PS51183. JMJN. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03833-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIKPVEVID GVPVFKPSMM EFANFQYFID EITKFGIENG IVKVIPPKEW
60 70 80 90 100
LELLEGSPPA ESLKTIQLDS PIQQQAKRWD KHENGVFSIE NEYDNKSYNL
110 120 130 140 150
TQWKNLAESL DSRISQGDFN DKTLKENCRV DSQQDCYDLA QLQILESDFW
160 170 180 190 200
KTIAFSKPFY AVDENSSIFP YDLTLWNLNN LPDSINSSNR RLLTGQSKCI
210 220 230 240 250
FPWHLDEQNK CSINYLHFGA PKQWYSIPSA NTDQFLKILS KEPSSNKENC
260 270 280 290 300
PAFIRHQNII TSPDFLRKNN IKFNRVVQFQ HEFIITFPYC MYSGFNYGYN
310 320 330 340 350
FGESIEFILD QQAVVRKQPL KCGCGNKKEE RKSGPFSNLS YDSNESEQRG
360 370 380 390 400
SITDNDNDLF QKVRSFDELL NHSSQELQNL EDNKNPLFSN INMNRPQSSS
410 420 430 440 450
LRSTTPNGVN QFLNMNQTTI SRISSPLLSR MMDLSNIVEP TLDDPGSKFK
460 470 480 490 500
RKVLTPQLPQ MNIPSNSSNF GTPSLTNTNS LLSNITATST NPSTTTNGSQ
510 520 530 540 550
NHNNVNANGI NTSAAASINN NISSTNNSAN NSSSNNNVST VPSSMMHSST
560 570 580 590 600
LNGTSGLGGD NDDNMLALSL ATLANSATAS PRLTLPPLSS PMNPNGHTSY
610 620 630 640 650
NGNMMNNNSG NGSNGSNSYS NGVTTAAATT TSAPHNLSIV SPNPTYSPNP
660 670 680 690 700
LSLYLTNSKN PLNSGLAPLS PSTSNIPFLK RNNVVTLNIS REASKSPISS
710 720 730 740 750
FVNDYRSPLG VSNPLMYSST INDYSNGTGI RQNSNNINPL DAGPSFSPLH
760 770 780 790 800
KKPKILNGND NSNLDSNNFD YSFTGNKQES NPSILNNNTN NNDNYRTSSM
810 820 830 840 850
NNNGNNYQAH SSKFGENEVI MSDHGKIYIC RECNRQFSSG HHLTRHKKSV
860 870 880 890
HSGEKPHSCP RCGKRFKRRD HVLQHLNKKI PCTQEMENTK LAES
Length:894
Mass (Da):99,481
Last modified:November 1, 1996 - v1
Checksum:i06F9F2B7F485DF88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47746 Genomic DNA. Translation: CAA87670.1.
BK006938 Genomic DNA. Translation: DAA11941.1.
PIRiS51245.
RefSeqiNP_010381.1. NM_001180404.1.

Genome annotation databases

EnsemblFungiiYDR096W; YDR096W; YDR096W.
GeneIDi851670.
KEGGisce:YDR096W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47746 Genomic DNA. Translation: CAA87670.1.
BK006938 Genomic DNA. Translation: DAA11941.1.
PIRiS51245.
RefSeqiNP_010381.1. NM_001180404.1.

3D structure databases

ProteinModelPortaliQ03833.
SMRiQ03833.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32151. 83 interactors.
IntActiQ03833. 33 interactors.
MINTiMINT-4480789.

PTM databases

iPTMnetiQ03833.

Proteomic databases

MaxQBiQ03833.
PRIDEiQ03833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR096W; YDR096W; YDR096W.
GeneIDi851670.
KEGGisce:YDR096W.

Organism-specific databases

EuPathDBiFungiDB:YDR096W.
SGDiS000002503. GIS1.

Phylogenomic databases

GeneTreeiENSGT00530000063342.
HOGENOMiHOG000112726.
KOiK19798.
OrthoDBiEOG092C2SZJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-29699-MONOMER.
ReactomeiR-SCE-3214842. HDMs demethylate histones.
R-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Miscellaneous databases

PROiQ03833.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR003347. JmjC_dom.
IPR003349. JmjN.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02373. JmjC. 1 hit.
PF02375. JmjN. 1 hit.
[Graphical view]
SMARTiSM00558. JmjC. 1 hit.
SM00545. JmjN. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
PS51183. JMJN. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGIS1_YEAST
AccessioniPrimary (citable) accession number: Q03833
Secondary accession number(s): D6VS81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.