Q03824 (INP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inheritance of peroxisomes protein 2

Gene names

Name:	INP2
Ordered Locus Names:	YMR163C
ORF Names:	YM8520.12C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for peroxisome inheritance. Acts as the peroxisome-specific receptor for the myosin V motor MYO2. Ref.5
Subunit structure	Interacts with MYO2. Ref.5
Subcellular location	Peroxisome membrane; Single-pass membrane protein Ref.3 Ref.5.
Miscellaneous	Present with 736 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the INP2 family.

Ontologies

Keywords
Cellular component	Membrane Peroxisome
Domain	Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peroxisome inheritance Inferred from genetic interaction Ref.5. Source: SGD
Cellular_component	integral to peroxisomal membrane Inferred from direct assay Ref.5. Source: SGD
Molecular_function	protein anchor Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MYO2	P19524	3	EBI-27354,EBI-11659
PEX19	Q07418	3	EBI-27354,EBI-292

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	Inheritance of peroxisomes protein 2		PRO_0000203314
Regions
	Transmembrane	215 – 231	17	Helical; Potential
Amino acid modifications
	Modified residue	74	1	Phosphothreonine Ref.6
	Glycosylation	275	1	N-linked (GlcNAc...) Potential
	Glycosylation	343	1	N-linked (GlcNAc...) Potential
	Glycosylation	344	1	N-linked (GlcNAc...) Potential
	Glycosylation	379	1	N-linked (GlcNAc...) Potential
	Glycosylation	517	1	N-linked (GlcNAc...) Potential
	Glycosylation	569	1	N-linked (GlcNAc...) Potential
	Glycosylation	574	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q03824 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: E4F2A4D205A98F66	FASTA	705	81,467
	10 20 30 40 50 60 MTTNSRPSAL QAPGLQIFSM LKSSEEDGFM SSSLTLDSDN IIGVTENNRQ EFYSTWRKPS 70 80 90 100 110 120 LLSSRSVLHE YSPTIVGSND CTFSPITVGK TTKFFNWDDI ISRIFMQQPF GVTHQFFEEF 130 140 150 160 170 180 RYSIITSHFL NDMNHYRLSL HLDQSIMNFH KSSTLLKNVP PKSVPFMATK YGKLAVAEDK 190 200 210 220 230 240 KLYFRQNFNY LSMIITSYRV LTQLKKYCRK KNSPGLKRVV ILILVAVYLS IQQEYFRRHL 250 260 270 280 290 300 ICYKTLLKVR KVLESLQQVD VMIHKYHLRF KEIKNHSFIS RVSLISIADE HSSVIKELLV 310 320 330 340 350 360 FSSDALFYKL KSIIPDIVIF SDTSELSKYC ELYGIDVPNL YYNNTTTVKD LDGKLYRLKL 370 380 390 400 410 420 LKKFMLCCLL SLDMTGNENL SNVNMRNALN KIFPDYMARV QLKKKYNPIG TFQNIVSLLR 430 440 450 460 470 480 GLHSLLSTVL VSLNDHKQIL YAFPEETSTN TGCERANVCS FSKNDKLFQA LNYLKMIENN 490 500 510 520 530 540 LLAIDIRNGI TENDRNIIED KLEELITFWK TSKICGNISR IQKVSPTNTI NHGFHLDILK 550 560 570 580 590 600 GRKSPRSSSV QGLSLERKVD FIDVAESVND SFENDTELEE YEDYDCQEEC SAGSRQNHRV 610 620 630 640 650 660 DFIGKDSCRK PDFKQLSDNE LRRKLDERIL KLAQENREGR ERLRTAKSFE LLRKAQASMS 670 680 690 700 VKFGFQKPLR DDAFLESRPL SKCKVSSEET IPFLYELKGL LGNDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The peroxisomal membrane protein Inp2p is the peroxisome-specific receptor for the myosin V motor Myo2p of Saccharomyces cerevisiae." Fagarasanu A., Fagarasanu M., Eitzen G.A., Aitchison J.D., Rachubinski R.A. Dev. Cell 10:587-600(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MYO2.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-74, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49705 Genomic DNA. Translation: CAA89799.1. BK006946 Genomic DNA. Translation: DAA10059.1.
PIR	S54521.
RefSeq	NP_013886.1. NM_001182667.1.
3D structure databases
ProteinModelPortal	Q03824.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1673N.
IntAct	Q03824. 5 interactions.
MINT	MINT-385667.
STRING	4932.YMR163C.
Proteomic databases
PaxDb	Q03824.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR163C; YMR163C; YMR163C.
GeneID	855198.
KEGG	sce:YMR163C.
Organism-specific databases
CYGD	YMR163c.
SGD	S000004773. INP2.
Phylogenomic databases
eggNOG	NOG40466.
OMA	ITHIASM.
OrthoDB	EOG46T6B3.
Gene expression databases
Genevestigator	Q03824.
GermOnline	YMR163C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR026235. INP2. IPR026859. Myosin-bd. [Graphical view]
Pfam	PF12632. Vezatin. 1 hit. [Graphical view]
PRINTS	PR02104. INPROXISOME2.
ProtoNet	Search...
Other
NextBio	978679.

Entry information

Entry name

INP2_YEAST

Accession

Primary (citable) accession number: Q03824
Secondary accession number(s): D6VZY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program