true1997-11-012024-03-27169ATG16_YEASTThe nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.Bowman S.Churcher C.M.Badcock K.Brown D.Chillingworth T.Connor R.Dedman K.Devlin K.Gentles S.Hamlin N.Hunt S.Jagels K.Lye G.Moule S.Odell C.Pearson D.Rajandream M.A.Rice P.Skelton J.Walsh S.V.Whitehead S.Barrell B.G.1997Nature38790-93NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]ATCC 204508 / S288cThe reference genome sequence of Saccharomyces cerevisiae: Then and now.Engel S.R.Dietrich F.S.Fisk D.G.Binkley G.Balakrishnan R.Costanzo M.C.Dwight S.S.Hitz B.C.Karra K.Nash R.S.Weng S.Wong E.D.Lloyd P.Skrzypek M.S.Miyasato S.R.Simison M.Cherry J.M.doi:10.1534/g3.113.0089952014G3 (Bethesda)4389-398GENOME REANNOTATIONIsolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae.Tsukada M.Ohsumi Y.doi:10.1016/0014-5793(93)80398-e1993FEBS Lett.333169-174FUNCTIONGenetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway.Harding T.M.Hefner-Gravink A.Thumm M.Klionsky D.J.doi:10.1074/jbc.271.30.176211996J. Biol. Chem.27117621-17624FUNCTIONApg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway.Mizushima N.Noda T.Ohsumi Y.doi:10.1093/emboj/18.14.38881999EMBO J.183888-3896FUNCTIONINTERACTION WITH ATG5 AND ATG12The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation.Suzuki K.Kirisako T.Kamada Y.Mizushima N.Noda T.Ohsumi Y.doi:10.1093/emboj/20.21.59712001EMBO J.205971-5981FUNCTIONSUBCELLULAR LOCATIONFormation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast.Kuma A.Mizushima N.Ishihara N.Ohsumi Y.doi:10.1074/jbc.m1118892002002J. Biol. Chem.27718619-18625FUNCTIONIDENTIFICATION IN THE COMPLEXA unified nomenclature for yeast autophagy-related genes.Klionsky D.J.Cregg J.M.Dunn W.A. Jr.Emr S.D.Sakai Y.Sandoval I.V.Sibirny A.Subramani S.Thumm M.Veenhuis M.Ohsumi Y.doi:10.1016/s1534-5807(03)00296-x2003Dev. Cell5539-545NOMENCLATUREGlobal analysis of protein expression in yeast.Ghaemmaghami S.Huh W.-K.Bower K.Howson R.W.Belle A.Dephoure N.O'Shea E.K.Weissman J.S.doi:10.1038/nature020462003Nature425737-741LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]apg15-1, a UGA mutant allele in the Saccharomyces cerevisiae APG16 gene, and its suppression by a cytoplasmic factor.Okazaki H.Ono B.Ohsumi Y.Ohsumi M.doi:10.1271/bbb.68.15412004Biosci. Biotechnol. Biochem.681541-1548PHENOTYPIC CHARACTERIZATIONStructure-function relationship of Atg12, a ubiquitin-like modifier essential for autophagy.Hanada T.Ohsumi Y.doi:10.4161/auto.1.2.18582005Autophagy1110-118IDENTIFICATION IN THE ATG5-ATG12/ATG16 COMPLEXStarvation triggers the delivery of the endoplasmic reticulum to the vacuole via autophagy in yeast.Hamasaki M.Noda T.Baba M.Ohsumi Y.doi:10.1111/j.1600-0854.2004.00245.x2005Traffic656-65FUNCTIONAutophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response.Bernales S.McDonald K.L.Walter P.doi:10.1371/journal.pbio.00404232006PLoS Biol.4E423FUNCTIONThe Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae.Cheong H.Nair U.Geng J.Klionsky D.J.doi:10.1091/mbc.e07-08-08262008Mol. Biol. Cell19668-681SUBCELLULAR LOCATIONRoles of the lipid-binding motifs of Atg18 and Atg21 in the cytoplasm to vacuole targeting pathway and autophagy.Nair U.Cao Y.Xie Z.Klionsky D.J.doi:10.1074/jbc.m109.0803742010J. Biol. Chem.28511476-11488SUBCELLULAR LOCATIONDual roles of Atg8-PE deconjugation by Atg4 in autophagy.Yu Z.Q.Ni T.Hong B.Wang H.Y.Jiang F.J.Zou S.Chen Y.Zheng X.L.Klionsky D.J.Liang Y.Xie Z.doi:10.4161/auto.196522012Autophagy8883-892FUNCTIONMechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation.Romanov J.Walczak M.Ibiricu I.Schuchner S.Ogris E.Kraft C.Martens S.doi:10.1038/emboj.2012.2782012EMBO J.314304-4317FUNCTIONSUBCELLULAR LOCATION OF THE ATG5-ATG2/ATG16 COMPLEXStructure of Atg5.Atg16, a complex essential for autophagy.Matsushita M.Suzuki N.N.Obara K.Fujioka Y.Ohsumi Y.Inagaki F.doi:10.1074/jbc.m6098762002007J. Biol. Chem.2826763-6772X-RAY CRYSTALLOGRAPHY (1.97 ANGSTROMS) OF 1-57 IN COMPLEX WITH ATG5MUTAGENESIS OF ARG-35 AND PHE-46FUNCTIONDimeric coiled-coil structure of Saccharomyces cerevisiae Atg16 and its functional significance in autophagy.Fujioka Y.Noda N.N.Nakatogawa H.Ohsumi Y.Inagaki F.doi:10.1074/jbc.m109.0535202010J. Biol. Chem.2851508-1515X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)SUBUNITMUTAGENESIS OF ASP-101; GLU-102; ILE-104; ILE-108 AND VAL-112Structure of the Atg12-Atg5 conjugate reveals a platform for stimulating Atg8-PE conjugation.Noda N.N.Fujioka Y.Hanada T.Ohsumi Y.Inagaki F.doi:10.1038/embor.2012.2082013EMBO Rep.14206-211X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 1-46 IN COMPLEX WITH ATG5 AND ATG122.20B/D/F/H=1-461.97B=1-572.50A/B/C/D/E/F=50-1232.80A/B=1-1502.60B=1-46108ATG12-ATG5-ATG16 complex8ATG16Eukaryota0 hits in 10 CRISPR screensProteinAtg16_CCDAtg16_NTDAutophagy-rel_prot_16_domATG16Autophagy protein 16Cytoplasm to vacuole targeting protein 11SAP18 homologATG16APG15APG16CVT11SAP18YMR159CYM8520.08CStabilizes the ATG5-ATG12 conjugate and mediates the formation of the 350 kDa complex, which is necessary for autophagy. The ATG5-ATG12/ATG16 complex is required for efficient promotion of ATG8-conjugation to phosphatidylethanolamine and ATG8 localization to the pre-autophagosomal structure (PAS). Recruits also ATG3 to the PAS. Involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress.Homodimer. Part of the 350 kDa complex which is at least composed of ATG5, ATG12 and ATG16. Several units of each may be present in this complex. Interacts directly with ATG12.Present with 573 molecules/cell in log phase SD medium.Belongs to the ATG16 family.Autophagy protein 16172221150Disordered2358130Basic and acidic residues7Impairs interaction with ATG5and autophagy.A35Impairs interaction with ATG5and autophagy.A46Significantly reduces autophagic activity; when associated with A-102.A101Significantly reduces autophagic activity; when associated with A-101.A102Significantly reduces autophagic activity; when associated with A-108 and A-112.A104Significantly reduces autophagic activity; when associated with A-104 and A-112.A108Significantly reduces autophagic activity; when associated with A-104 and A-108.A112404345485161117false10false3false4ATG16ATG12ATG51997-11-01117222f77c1264a185eb85d099e720b09b1bb2MGNFIITERKKAKEERSNPQTDSMDDLLIRRLTDRNDKEAHLNELFQDNSGAIGGNIVSHDDALLNTLAILQKELKSKEQEIRRLKEVIALKNKNTERLNDELISGTIENNVLQQKLSDLKKEHSQLVARWLKKTEKETEAMNSEIDGTKtruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetrue