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Q03818

- ATG16_YEAST

UniProt

Q03818 - ATG16_YEAST

Protein

Autophagy protein 16

Gene

ATG16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Stabilizes the ATG5-ATG12 conjugate and mediates the formation of the 350 kDa complex, which is necessary for autophagy. The ATG5-ATG12/ATG16 complex is required for efficient promotion of ATG8-conjugation to phosphatidylethanolamine and ATG8 localization to the pre-autophagosomal structure (PAS). Recruites also ATG3 to the PAS. Involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress.10 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct
    3. protein binding, bridging Source: SGD

    GO - Biological processi

    1. autophagy Source: UniProtKB
    2. C-terminal protein lipidation Source: SGD
    3. CVT pathway Source: SGD
    4. late nucleophagy Source: SGD
    5. macroautophagy Source: SGD
    6. mitochondrion degradation Source: SGD
    7. piecemeal microautophagy of nucleus Source: SGD

    Keywords - Biological processi

    Autophagy, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32849-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Autophagy protein 16
    Alternative name(s):
    Cytoplasm to vacuole targeting protein 11
    SAP18 homolog
    Gene namesi
    Name:ATG16
    Synonyms:APG15, APG16, CVT11, SAP18
    Ordered Locus Names:YMR159C
    ORF Names:YM8520.08C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR159c.
    SGDiS000004769. ATG16.

    Subcellular locationi

    Preautophagosomal structure membrane 4 Publications; Peripheral membrane protein 4 Publications

    GO - Cellular componenti

    1. Atg12-Atg5-Atg16 complex Source: SGD
    2. pre-autophagosomal structure Source: SGD
    3. pre-autophagosomal structure membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi35 – 351R → A: Impairs interaction with ATG5and autophagy. 1 Publication
    Mutagenesisi46 – 461F → A: Impairs interaction with ATG5and autophagy. 1 Publication
    Mutagenesisi101 – 1011D → A: Significantly reduces autophagic activity; when associated with A-102. 1 Publication
    Mutagenesisi102 – 1021E → A: Significantly reduces autophagic activity; when associated with A-101. 1 Publication
    Mutagenesisi104 – 1041I → A: Significantly reduces autophagic activity; when associated with A-108 and A-112. 1 Publication
    Mutagenesisi108 – 1081I → A: Significantly reduces autophagic activity; when associated with A-104 and A-112. 1 Publication
    Mutagenesisi112 – 1121V → A: Significantly reduces autophagic activity; when associated with A-104 and A-108. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 150150Autophagy protein 16PRO_0000218595Add
    BLAST

    Proteomic databases

    PaxDbiQ03818.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03818.

    Interactioni

    Subunit structurei

    Homodimer. Part of the 350 kDa complex which is at least composed of ATG5, ATG12 and ATG16. Several units of each may be present in this complex. Interacts directly with ATG12.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-27344,EBI-27344
    ATG12P3831611EBI-27344,EBI-2692
    ATG5Q123805EBI-27344,EBI-2664
    MEC3Q025743EBI-27344,EBI-10658

    Protein-protein interaction databases

    BioGridi35336. 40 interactions.
    DIPiDIP-2144N.
    IntActiQ03818. 8 interactions.
    MINTiMINT-406657.
    STRINGi4932.YMR159C.

    Structurei

    Secondary structure

    1
    150
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi23 – 4018
    Helixi43 – 453
    Turni48 – 514
    Helixi61 – 11757

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DYMX-ray2.20B/D/F/H1-46[»]
    2DYOX-ray1.97B1-57[»]
    3A7OX-ray2.50A/B/C/D/E/F50-123[»]
    3A7PX-ray2.80A/B1-150[»]
    3W1SX-ray2.60B1-46[»]
    ProteinModelPortaliQ03818.
    SMRiQ03818. Positions 22-57, 60-119.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ03818.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili58 – 13073Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATG16 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG83585.
    HOGENOMiHOG000034134.
    KOiK08340.
    OrthoDBiEOG7K9KGP.

    Family and domain databases

    InterProiIPR013923. Autophagy-rel_prot_16.
    [Graphical view]
    PfamiPF08614. ATG16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03818-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNFIITERK KAKEERSNPQ TDSMDDLLIR RLTDRNDKEA HLNELFQDNS    50
    GAIGGNIVSH DDALLNTLAI LQKELKSKEQ EIRRLKEVIA LKNKNTERLN 100
    DELISGTIEN NVLQQKLSDL KKEHSQLVAR WLKKTEKETE AMNSEIDGTK 150
    Length:150
    Mass (Da):17,222
    Last modified:November 1, 1997 - v1
    Checksum:i6D4308298D4DBB59
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49705 Genomic DNA. Translation: CAA89795.1.
    BK006946 Genomic DNA. Translation: DAA10055.1.
    PIRiS54517.
    RefSeqiNP_013882.1. NM_001182663.1.

    Genome annotation databases

    EnsemblFungiiYMR159C; YMR159C; YMR159C.
    GeneIDi855194.
    KEGGisce:YMR159C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49705 Genomic DNA. Translation: CAA89795.1 .
    BK006946 Genomic DNA. Translation: DAA10055.1 .
    PIRi S54517.
    RefSeqi NP_013882.1. NM_001182663.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DYM X-ray 2.20 B/D/F/H 1-46 [» ]
    2DYO X-ray 1.97 B 1-57 [» ]
    3A7O X-ray 2.50 A/B/C/D/E/F 50-123 [» ]
    3A7P X-ray 2.80 A/B 1-150 [» ]
    3W1S X-ray 2.60 B 1-46 [» ]
    ProteinModelPortali Q03818.
    SMRi Q03818. Positions 22-57, 60-119.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35336. 40 interactions.
    DIPi DIP-2144N.
    IntActi Q03818. 8 interactions.
    MINTi MINT-406657.
    STRINGi 4932.YMR159C.

    Proteomic databases

    PaxDbi Q03818.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR159C ; YMR159C ; YMR159C .
    GeneIDi 855194.
    KEGGi sce:YMR159C.

    Organism-specific databases

    CYGDi YMR159c.
    SGDi S000004769. ATG16.

    Phylogenomic databases

    eggNOGi NOG83585.
    HOGENOMi HOG000034134.
    KOi K08340.
    OrthoDBi EOG7K9KGP.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32849-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q03818.
    NextBioi 978667.

    Gene expression databases

    Genevestigatori Q03818.

    Family and domain databases

    InterProi IPR013923. Autophagy-rel_prot_16.
    [Graphical view ]
    Pfami PF08614. ATG16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
      Tsukada M., Ohsumi Y.
      FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway."
      Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J.
      J. Biol. Chem. 271:17621-17624(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway."
      Mizushima N., Noda T., Ohsumi Y.
      EMBO J. 18:3888-3896(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATG5 AND ATG12.
    6. "The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
      Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
      EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast."
      Kuma A., Mizushima N., Ishihara N., Ohsumi Y.
      J. Biol. Chem. 277:18619-18625(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE COMPLEX.
    8. Cited for: NOMENCLATURE.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "apg15-1, a UGA mutant allele in the Saccharomyces cerevisiae APG16 gene, and its suppression by a cytoplasmic factor."
      Okazaki H., Ono B., Ohsumi Y., Ohsumi M.
      Biosci. Biotechnol. Biochem. 68:1541-1548(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHENOTYPIC CHARACTERIZATION.
    11. "Structure-function relationship of Atg12, a ubiquitin-like modifier essential for autophagy."
      Hanada T., Ohsumi Y.
      Autophagy 1:110-118(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE ATG5-ATG12/ATG16 COMPLEX.
    12. "Starvation triggers the delivery of the endoplasmic reticulum to the vacuole via autophagy in yeast."
      Hamasaki M., Noda T., Baba M., Ohsumi Y.
      Traffic 6:56-65(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response."
      Bernales S., McDonald K.L., Walter P.
      PLoS Biol. 4:E423-E423(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae."
      Cheong H., Nair U., Geng J., Klionsky D.J.
      Mol. Biol. Cell 19:668-681(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    15. "Roles of the lipid-binding motifs of Atg18 and Atg21 in the cytoplasm to vacuole targeting pathway and autophagy."
      Nair U., Cao Y., Xie Z., Klionsky D.J.
      J. Biol. Chem. 285:11476-11488(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    16. Cited for: FUNCTION.
    17. "Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation."
      Romanov J., Walczak M., Ibiricu I., Schuchner S., Ogris E., Kraft C., Martens S.
      EMBO J. 31:4304-4317(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION OF THE ATG5-ATG2/ATG16 COMPLEX.
    18. "Structure of Atg5.Atg16, a complex essential for autophagy."
      Matsushita M., Suzuki N.N., Obara K., Fujioka Y., Ohsumi Y., Inagaki F.
      J. Biol. Chem. 282:6763-6772(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.97 ANGSTROMS) OF 1-57 IN COMPLEX WITH ATG5, MUTAGENESIS OF ARG-35 AND PHE-46, FUNCTION.
    19. "Dimeric coiled-coil structure of Saccharomyces cerevisiae Atg16 and its functional significance in autophagy."
      Fujioka Y., Noda N.N., Nakatogawa H., Ohsumi Y., Inagaki F.
      J. Biol. Chem. 285:1508-1515(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), SUBUNIT, MUTAGENESIS OF ASP-101; GLU-102; ILE-104; ILE-108 AND VAL-112.
    20. "Structure of the Atg12-Atg5 conjugate reveals a platform for stimulating Atg8-PE conjugation."
      Noda N.N., Fujioka Y., Hanada T., Ohsumi Y., Inagaki F.
      EMBO Rep. 14:206-211(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 1-46 IN COMPLEX WITH ATG5 AND ATG12.

    Entry informationi

    Entry nameiATG16_YEAST
    AccessioniPrimary (citable) accession number: Q03818
    Secondary accession number(s): D6VZY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 573 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3