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Q03818 (ATG16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Autophagy protein 16
Alternative name(s):
Cytoplasm to vacuole targeting protein 11
SAP18 homolog
Gene names
Name:ATG16
Synonyms:APG15, APG16, CVT11, SAP18
Ordered Locus Names:YMR159C
ORF Names:YM8520.08C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length150 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stabilizes the ATG5-ATG12 conjugate and mediates the formation of the 350 kDa complex, which is necessary for autophagy. The ATG5-ATG12/ATG16 complex is required for efficient promotion of ATG8-conjugation to phosphatidylethanolamine and ATG8 localization to the pre-autophagosomal structure (PAS). Recruites also ATG3 to the PAS. Involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.12 Ref.13 Ref.16 Ref.17 Ref.18

Subunit structure

Homodimer. Part of the 350 kDa complex which is at least composed of ATG5, ATG12 and ATG16. Several units of each may be present in this complex. Interacts directly with ATG12. Ref.5 Ref.7 Ref.11 Ref.19

Subcellular location

Preautophagosomal structure membrane; Peripheral membrane protein Ref.6 Ref.14 Ref.15 Ref.17.

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ATG16 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 150150Autophagy protein 16
PRO_0000218595

Regions

Coiled coil58 – 13073 Potential

Experimental info

Mutagenesis351R → A: Impairs interaction with ATG5and autophagy. Ref.18
Mutagenesis461F → A: Impairs interaction with ATG5and autophagy. Ref.18
Mutagenesis1011D → A: Significantly reduces autophagic activity; when associated with A-102. Ref.19
Mutagenesis1021E → A: Significantly reduces autophagic activity; when associated with A-101. Ref.19
Mutagenesis1041I → A: Significantly reduces autophagic activity; when associated with A-108 and A-112. Ref.19
Mutagenesis1081I → A: Significantly reduces autophagic activity; when associated with A-104 and A-112. Ref.19
Mutagenesis1121V → A: Significantly reduces autophagic activity; when associated with A-104 and A-108. Ref.19

Secondary structure

......... 150
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q03818 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 6D4308298D4DBB59

FASTA15017,222
        10         20         30         40         50         60 
MGNFIITERK KAKEERSNPQ TDSMDDLLIR RLTDRNDKEA HLNELFQDNS GAIGGNIVSH 

        70         80         90        100        110        120 
DDALLNTLAI LQKELKSKEQ EIRRLKEVIA LKNKNTERLN DELISGTIEN NVLQQKLSDL 

       130        140        150 
KKEHSQLVAR WLKKTEKETE AMNSEIDGTK 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
Tsukada M., Ohsumi Y.
FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway."
Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J.
J. Biol. Chem. 271:17621-17624(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway."
Mizushima N., Noda T., Ohsumi Y.
EMBO J. 18:3888-3896(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ATG5 AND ATG12.
[6]"The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast."
Kuma A., Mizushima N., Ishihara N., Ohsumi Y.
J. Biol. Chem. 277:18619-18625(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE COMPLEX.
[8]"A unified nomenclature for yeast autophagy-related genes."
Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y.
Dev. Cell 5:539-545(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"apg15-1, a UGA mutant allele in the Saccharomyces cerevisiae APG16 gene, and its suppression by a cytoplasmic factor."
Okazaki H., Ono B., Ohsumi Y., Ohsumi M.
Biosci. Biotechnol. Biochem. 68:1541-1548(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHENOTYPIC CHARACTERIZATION.
[11]"Structure-function relationship of Atg12, a ubiquitin-like modifier essential for autophagy."
Hanada T., Ohsumi Y.
Autophagy 1:110-118(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE ATG5-ATG12/ATG16 COMPLEX.
[12]"Starvation triggers the delivery of the endoplasmic reticulum to the vacuole via autophagy in yeast."
Hamasaki M., Noda T., Baba M., Ohsumi Y.
Traffic 6:56-65(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response."
Bernales S., McDonald K.L., Walter P.
PLoS Biol. 4:E423-E423(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae."
Cheong H., Nair U., Geng J., Klionsky D.J.
Mol. Biol. Cell 19:668-681(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[15]"Roles of the lipid-binding motifs of Atg18 and Atg21 in the cytoplasm to vacuole targeting pathway and autophagy."
Nair U., Cao Y., Xie Z., Klionsky D.J.
J. Biol. Chem. 285:11476-11488(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[16]"Dual roles of Atg8-PE deconjugation by Atg4 in autophagy."
Yu Z.Q., Ni T., Hong B., Wang H.Y., Jiang F.J., Zou S., Chen Y., Zheng X.L., Klionsky D.J., Liang Y., Xie Z.
Autophagy 8:883-892(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[17]"Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation."
Romanov J., Walczak M., Ibiricu I., Schuchner S., Ogris E., Kraft C., Martens S.
EMBO J. 31:4304-4317(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION OF THE ATG5-ATG2/ATG16 COMPLEX.
[18]"Structure of Atg5.Atg16, a complex essential for autophagy."
Matsushita M., Suzuki N.N., Obara K., Fujioka Y., Ohsumi Y., Inagaki F.
J. Biol. Chem. 282:6763-6772(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.97 ANGSTROMS) OF 1-57 IN COMPLEX WITH ATG5, MUTAGENESIS OF ARG-35 AND PHE-46, FUNCTION.
[19]"Dimeric coiled-coil structure of Saccharomyces cerevisiae Atg16 and its functional significance in autophagy."
Fujioka Y., Noda N.N., Nakatogawa H., Ohsumi Y., Inagaki F.
J. Biol. Chem. 285:1508-1515(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), SUBUNIT, MUTAGENESIS OF ASP-101; GLU-102; ILE-104; ILE-108 AND VAL-112.
[20]"Structure of the Atg12-Atg5 conjugate reveals a platform for stimulating Atg8-PE conjugation."
Noda N.N., Fujioka Y., Hanada T., Ohsumi Y., Inagaki F.
EMBO Rep. 14:206-211(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 1-46 IN COMPLEX WITH ATG5 AND ATG12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49705 Genomic DNA. Translation: CAA89795.1.
BK006946 Genomic DNA. Translation: DAA10055.1.
PIRS54517.
RefSeqNP_013882.1. NM_001182663.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DYMX-ray2.20B/D/F/H1-46[»]
2DYOX-ray1.97B1-57[»]
3A7OX-ray2.50A/B/C/D/E/F50-123[»]
3A7PX-ray2.80A/B1-150[»]
3W1SX-ray2.60B1-46[»]
ProteinModelPortalQ03818.
SMRQ03818. Positions 22-57, 60-119.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35336. 33 interactions.
DIPDIP-2144N.
IntActQ03818. 8 interactions.
MINTMINT-406657.
STRING4932.YMR159C.

Proteomic databases

PaxDbQ03818.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR159C; YMR159C; YMR159C.
GeneID855194.
KEGGsce:YMR159C.

Organism-specific databases

CYGDYMR159c.
SGDS000004769. ATG16.

Phylogenomic databases

eggNOGNOG83585.
HOGENOMHOG000034134.
KOK08340.
OrthoDBEOG7K9KGP.

Enzyme and pathway databases

BioCycYEAST:G3O-32849-MONOMER.

Gene expression databases

GenevestigatorQ03818.

Family and domain databases

InterProIPR013923. Autophagy-rel_prot_16.
[Graphical view]
PfamPF08614. ATG16. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ03818.
NextBio978667.

Entry information

Entry nameATG16_YEAST
AccessionPrimary (citable) accession number: Q03818
Secondary accession number(s): D6VZY1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references