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Protein

Autophagy protein 16

Gene

ATG16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stabilizes the ATG5-ATG12 conjugate and mediates the formation of the 350 kDa complex, which is necessary for autophagy. The ATG5-ATG12/ATG16 complex is required for efficient promotion of ATG8-conjugation to phosphatidylethanolamine and ATG8 localization to the pre-autophagosomal structure (PAS). Recruits also ATG3 to the PAS. Involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress.10 Publications

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein binding, bridging Source: SGD

GO - Biological processi

  • autophagy Source: UniProtKB
  • C-terminal protein lipidation Source: SGD
  • CVT pathway Source: SGD
  • late nucleophagy Source: SGD
  • macroautophagy Source: SGD
  • mitophagy Source: SGD
  • piecemeal microautophagy of nucleus Source: SGD

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32849-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy protein 16
Alternative name(s):
Cytoplasm to vacuole targeting protein 11
SAP18 homolog
Gene namesi
Name:ATG16
Synonyms:APG15, APG16, CVT11, SAP18
Ordered Locus Names:YMR159C
ORF Names:YM8520.08C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR159C.
SGDiS000004769. ATG16.

Subcellular locationi

GO - Cellular componenti

  • Atg12-Atg5-Atg16 complex Source: SGD
  • pre-autophagosomal structure Source: SGD
  • pre-autophagosomal structure membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi35R → A: Impairs interaction with ATG5and autophagy. 1 Publication1
Mutagenesisi46F → A: Impairs interaction with ATG5and autophagy. 1 Publication1
Mutagenesisi101D → A: Significantly reduces autophagic activity; when associated with A-102. 1 Publication1
Mutagenesisi102E → A: Significantly reduces autophagic activity; when associated with A-101. 1 Publication1
Mutagenesisi104I → A: Significantly reduces autophagic activity; when associated with A-108 and A-112. 1 Publication1
Mutagenesisi108I → A: Significantly reduces autophagic activity; when associated with A-104 and A-112. 1 Publication1
Mutagenesisi112V → A: Significantly reduces autophagic activity; when associated with A-104 and A-108. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002185951 – 150Autophagy protein 16Add BLAST150

Proteomic databases

PRIDEiQ03818.

Interactioni

Subunit structurei

Homodimer. Part of the 350 kDa complex which is at least composed of ATG5, ATG12 and ATG16. Several units of each may be present in this complex. Interacts directly with ATG12.6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi35336. 100 interactors.
DIPiDIP-2144N.
IntActiQ03818. 8 interactors.
MINTiMINT-406657.

Structurei

Secondary structure

1150
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 40Combined sources18
Helixi43 – 45Combined sources3
Turni48 – 51Combined sources4
Helixi61 – 117Combined sources57

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DYMX-ray2.20B/D/F/H1-46[»]
2DYOX-ray1.97B1-57[»]
3A7OX-ray2.50A/B/C/D/E/F50-123[»]
3A7PX-ray2.80A/B1-150[»]
3W1SX-ray2.60B1-46[»]
ProteinModelPortaliQ03818.
SMRiQ03818.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03818.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili58 – 130Sequence analysisAdd BLAST73

Sequence similaritiesi

Belongs to the ATG16 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000034134.
InParanoidiQ03818.
KOiK08340.
OrthoDBiEOG092C5PK2.

Family and domain databases

InterProiView protein in InterPro
IPR013923. Autophagy-rel_prot_16.
PfamiView protein in Pfam
PF08614. ATG16. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNFIITERK KAKEERSNPQ TDSMDDLLIR RLTDRNDKEA HLNELFQDNS
60 70 80 90 100
GAIGGNIVSH DDALLNTLAI LQKELKSKEQ EIRRLKEVIA LKNKNTERLN
110 120 130 140 150
DELISGTIEN NVLQQKLSDL KKEHSQLVAR WLKKTEKETE AMNSEIDGTK
Length:150
Mass (Da):17,222
Last modified:November 1, 1997 - v1
Checksum:i6D4308298D4DBB59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49705 Genomic DNA. Translation: CAA89795.1.
BK006946 Genomic DNA. Translation: DAA10055.1.
PIRiS54517.
RefSeqiNP_013882.1. NM_001182663.1.

Genome annotation databases

EnsemblFungiiYMR159C; YMR159C; YMR159C.
GeneIDi855194.
KEGGisce:YMR159C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49705 Genomic DNA. Translation: CAA89795.1.
BK006946 Genomic DNA. Translation: DAA10055.1.
PIRiS54517.
RefSeqiNP_013882.1. NM_001182663.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DYMX-ray2.20B/D/F/H1-46[»]
2DYOX-ray1.97B1-57[»]
3A7OX-ray2.50A/B/C/D/E/F50-123[»]
3A7PX-ray2.80A/B1-150[»]
3W1SX-ray2.60B1-46[»]
ProteinModelPortaliQ03818.
SMRiQ03818.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35336. 100 interactors.
DIPiDIP-2144N.
IntActiQ03818. 8 interactors.
MINTiMINT-406657.

Proteomic databases

PRIDEiQ03818.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR159C; YMR159C; YMR159C.
GeneIDi855194.
KEGGisce:YMR159C.

Organism-specific databases

EuPathDBiFungiDB:YMR159C.
SGDiS000004769. ATG16.

Phylogenomic databases

HOGENOMiHOG000034134.
InParanoidiQ03818.
KOiK08340.
OrthoDBiEOG092C5PK2.

Enzyme and pathway databases

BioCyciYEAST:G3O-32849-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ03818.
PROiPR:Q03818.

Family and domain databases

InterProiView protein in InterPro
IPR013923. Autophagy-rel_prot_16.
PfamiView protein in Pfam
PF08614. ATG16. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiATG16_YEAST
AccessioniPrimary (citable) accession number: Q03818
Secondary accession number(s): D6VZY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 15, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.