Autophagy protein 16 - Saccharomyces cerevisiae (Baker's yeast)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q03818 (ATG16_YEAST)

Last modified November 24, 2009. Version 66. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Autophagy protein 16
Alternative name(s):
    Cytoplasm to vacuole targeting protein 11
    SAP18 homolog

Gene names

Name:	ATG16
Synonyms:	APG15, APG16, CVT11, SAP18
Ordered Locus Names:	YMR159C
ORF Names:	YM8520.08C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Stabilizes the ATG5-ATG12 conjugate and mediates the formation of the 350 kDa complex, which is necessary for autophagy. Required for ATG8 localization to the pre-autophagosomal structure. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6
Subunit structure	Homooligomer. Part of the 350 kDa complex which is at least composed of ATG5, ATG12 and ATG16. Several units of each may be present in this complex. Interacts directly with ATG12. Ref.4
Subcellular location	Vacuole Potential. Cytoplasmic vesicle Potential. Note: Vesicular and vacuolar Potential. Found in punctate structures.
Miscellaneous	Present with 573 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the ATG16 family.

Hide | Top

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasmic vesicle Vacuole
Domain	Coiled coil
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	macroautophagy Ref.6 Inferred from direct assay. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Atg12-Atg5-Atg16 complex Ref.4 Ref.6 Inferred from direct assay. Source: SGD cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Ref.4 Inferred from direct assay. Source: SGD pre-autophagosomal structure Ref.5 Inferred from direct assay. Source: SGD vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct protein binding, bridging Ref.6 Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct
itself		2	EBI-27344,EBI-27344
ATG12	P38316	1	EBI-27344,EBI-2692
ATG5	Q12380	3	EBI-27344,EBI-2664
MEC3	Q02574	3	EBI-27344,EBI-10658

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 150

150

Autophagy protein 16

PRO_0000218595

Regions

Coiled coil

58 – 130

Potential

Secondary structure

150

Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q03818-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 6D4308298D4DBB59
Show »

FASTA

150

17,222

        10         20         30         40         50         60 
MGNFIITERK KAKEERSNPQ TDSMDDLLIR RLTDRNDKEA HLNELFQDNS GAIGGNIVSH 

        70         80         90        100        110        120 
DDALLNTLAI LQKELKSKEQ EIRRLKEVIA LKNKNTERLN DELISGTIEN NVLQQKLSDL 

       130        140        150 
KKEHSQLVAR WLKKTEKETE AMNSEIDGTK

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae." Tsukada M., Ohsumi Y. FEBS Lett. 333:169-174(1993) [PubMed: 8224160] [Abstract] Cited for: FUNCTION.
[3]	"Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway." Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J. J. Biol. Chem. 271:17621-17624(1996) [PubMed: 8663607] [Abstract] Cited for: FUNCTION.
[4]	"Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway." Mizushima N., Noda T., Ohsumi Y. EMBO J. 18:3888-3896(1999) [PubMed: 10406794] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATG5 AND ATG12.
[5]	"The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation." Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y. EMBO J. 20:5971-5981(2001) [PubMed: 11689437] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast." Kuma A., Mizushima N., Ishihara N., Ohsumi Y. J. Biol. Chem. 277:18619-18625(2002) [PubMed: 11897782] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE COMPLEX.
[7]	"A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract] Cited for: NOMENCLATURE.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"apg15-1, a UGA mutant allele in the Saccharomyces cerevisiae APG16 gene, and its suppression by a cytoplasmic factor." Okazaki H., Ono B., Ohsumi Y., Ohsumi M. Biosci. Biotechnol. Biochem. 68:1541-1548(2004) [PubMed: 15277759] [Abstract] Cited for: PHENOTYPIC CHARACTERIZATION.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

Z49705 Genomic DNA. Translation: CAA89795.1.

PIR

S54517.

RefSeq

NP_013882.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DYM	X-ray	2.20	B/D/F/H	1-46	[»]
2DYO	X-ray	1.97	B	1-57	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2144N.

IntAct

Q03818. 16 interactions.

STRING

Q03818.

Genome annotation databases

Ensembl

YMR159C; YMR159C; YMR159C; Saccharomyces cerevisiae. [Genome view]

GeneID

855194.

KEGG

sce:YMR159C.

NMPDR

fig|4932.3.peg.4932.

Organism-specific databases

CYGD

YMR159c.

SGD

S000004769. ATG16.

Phylogenomic databases

HOGENOM

Q03818.

OrthoDB

EOG9QRJMH

Gene expression databases

ArrayExpress

Q03818.

Genevestigator

Q03818.

GermOnline

YMR159C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR013923. Autophagy-rel_prot_16.
[Graphical view]

Pfam

PF08614. ATG16. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

978667.

Hide | Top

Entry information

Entry name

ATG16_YEAST

Accession

Primary (citable) accession number: Q03818

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Hide | Top

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents