ID   RT08_YEAST              Reviewed;         155 AA.
AC   Q03799; D6VZX9;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   27-MAR-2024, entry version 164.
DE   RecName: Full=Small ribosomal subunit protein uS8m {ECO:0000303|PubMed:28154081};
DE   AltName: Full=37S ribosomal protein S8, mitochondrial;
GN   Name=MRPS8; OrderedLocusNames=YMR158W; ORFNames=YM8520.07;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=12392552; DOI=10.1046/j.1432-1033.2002.03226.x;
RA   Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.;
RT   "Tag-mediated isolation of yeast mitochondrial ribosome and mass
RT   spectrometric identification of its new components.";
RL   Eur. J. Biochem. 269:5203-5214(2002).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 76625 / YPH499;
RX   PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA   Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA   Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA   Pfanner N., Meisinger C.;
RT   "The proteome of Saccharomyces cerevisiae mitochondria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN   [6]
RP   SUBCELLULAR LOCATION.
RX   PubMed=25609543; DOI=10.1038/ncomms7019;
RA   Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.;
RT   "Organization of the mitochondrial translation machinery studied in situ by
RT   cryoelectron tomography.";
RL   Nat. Commun. 6:6019-6019(2015).
RN   [7]
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.25 ANGSTROMS), AND SUBUNIT.
RX   PubMed=28154081; DOI=10.1126/science.aal2415;
RA   Desai N., Brown A., Amunts A., Ramakrishnan V.;
RT   "The structure of the yeast mitochondrial ribosome.";
RL   Science 355:528-531(2017).
CC   -!- FUNCTION: Component of the mitochondrial ribosome (mitoribosome), a
CC       dedicated translation machinery responsible for the synthesis of
CC       mitochondrial genome-encoded proteins, including at least some of the
CC       essential transmembrane subunits of the mitochondrial respiratory
CC       chain. The mitoribosomes are attached to the mitochondrial inner
CC       membrane and translation products are cotranslationally integrated into
CC       the membrane. {ECO:0000305|PubMed:25609543,
CC       ECO:0000305|PubMed:28154081}.
CC   -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt-
CC       SSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S)
CC       and a large (54S) subunit. The 37S small subunit contains a 15S
CC       ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large
CC       subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.
CC       {ECO:0000269|PubMed:12392552, ECO:0000269|PubMed:28154081}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14576278}.
CC       Note=Mitoribosomes are tethered to the mitochondrial inner membrane and
CC       spatially aligned with the membrane insertion machinery through two
CC       distinct membrane contact sites, formed by the 21S rRNA expansion
CC       segment 96-ES1 and the inner membrane protein MBA1.
CC       {ECO:0000269|PubMed:25609543}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS8 family.
CC       {ECO:0000305}.
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DR   EMBL; Z49705; CAA89794.1; -; Genomic_DNA.
DR   EMBL; AY558414; AAS56740.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA10053.1; -; Genomic_DNA.
DR   PIR; S54516; S54516.
DR   RefSeq; NP_013879.1; NM_001182661.1.
DR   PDB; 5MRC; EM; 3.25 A; HH=2-155.
DR   PDB; 5MRE; EM; 3.75 A; HH=2-155.
DR   PDB; 5MRF; EM; 4.97 A; HH=2-155.
DR   PDB; 8D8J; EM; 3.80 A; H=1-155.
DR   PDB; 8D8K; EM; 3.13 A; H=1-155.
DR   PDB; 8D8L; EM; 2.60 A; H=1-155.
DR   PDBsum; 5MRC; -.
DR   PDBsum; 5MRE; -.
DR   PDBsum; 5MRF; -.
DR   PDBsum; 8D8J; -.
DR   PDBsum; 8D8K; -.
DR   PDBsum; 8D8L; -.
DR   AlphaFoldDB; Q03799; -.
DR   EMDB; EMD-3551; -.
DR   EMDB; EMD-3552; -.
DR   EMDB; EMD-3553; -.
DR   SMR; Q03799; -.
DR   BioGRID; 35333; 67.
DR   ComplexPortal; CPX-1603; 37S mitochondrial small ribosomal subunit.
DR   DIP; DIP-6673N; -.
DR   IntAct; Q03799; 8.
DR   MINT; Q03799; -.
DR   STRING; 4932.YMR158W; -.
DR   MaxQB; Q03799; -.
DR   PaxDb; 4932-YMR158W; -.
DR   PeptideAtlas; Q03799; -.
DR   EnsemblFungi; YMR158W_mRNA; YMR158W; YMR158W.
DR   GeneID; 855190; -.
DR   KEGG; sce:YMR158W; -.
DR   AGR; SGD:S000004767; -.
DR   SGD; S000004767; MRPS8.
DR   VEuPathDB; FungiDB:YMR158W; -.
DR   eggNOG; ENOG502RXRN; Eukaryota.
DR   GeneTree; ENSGT00950000183198; -.
DR   HOGENOM; CLU_107213_0_0_1; -.
DR   InParanoid; Q03799; -.
DR   OMA; KYWQNEP; -.
DR   OrthoDB; 1818776at2759; -.
DR   BioCyc; YEAST:G3O-32848-MONOMER; -.
DR   BioGRID-ORCS; 855190; 0 hits in 10 CRISPR screens.
DR   PRO; PR:Q03799; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; Q03799; Protein.
DR   GO; GO:0005743; C:mitochondrial inner membrane; NAS:ComplexPortal.
DR   GO; GO:0005763; C:mitochondrial small ribosomal subunit; IDA:SGD.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR   GO; GO:0032543; P:mitochondrial translation; NAS:ComplexPortal.
DR   Gene3D; 3.30.1370.30; -; 1.
DR   Gene3D; 3.30.1490.10; -; 1.
DR   InterPro; IPR000630; Ribosomal_uS8.
DR   InterPro; IPR035987; Ribosomal_uS8_sf.
DR   PANTHER; PTHR11758:SF4; 37S RIBOSOMAL PROTEIN S8, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR11758; 40S RIBOSOMAL PROTEIN S15A; 1.
DR   Pfam; PF00410; Ribosomal_S8; 1.
DR   SUPFAM; SSF56047; Ribosomal protein S8; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Mitochondrion; Reference proteome; Ribonucleoprotein;
KW   Ribosomal protein.
FT   CHAIN           1..155
FT                   /note="Small ribosomal subunit protein uS8m"
FT                   /id="PRO_0000126599"
FT   HELIX           3..19
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   STRAND          21..27
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   HELIX           30..41
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   STRAND          44..56
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   TURN            65..67
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   HELIX           68..70
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   STRAND          71..77
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   STRAND          83..85
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   STRAND          89..94
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   STRAND          95..97
FT                   /evidence="ECO:0007829|PDB:8D8K"
FT   HELIX           103..110
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   STRAND          126..131
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   STRAND          134..137
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   HELIX           138..143
FT                   /evidence="ECO:0007829|PDB:8D8L"
FT   STRAND          148..154
FT                   /evidence="ECO:0007829|PDB:8D8L"
SQ   SEQUENCE   155 AA;  17471 MW;  2C1C7F021627567A CRC64;
     MSLVKLANTC AHLQNCSKVR VALTSIPYTK LQLQFAYNLY QQGFLSSLQK GSTMGPDKDF
     VEVTPDNIST RRLWVGLKYR DNKPVLSSCK LISKPNSRIH LPMEDMKKLC SGVTIRNIKP
     LQPGELILVR AHNNIMDINE AISKKLDGEV LCRVK
//