Q03796 (TPP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polynucleotide 3'-phosphatase
EC=3.1.3.32

Alternative name(s):
2'(3')-polynucleotidase
DNA 3'-phosphatase
Three prime phosphatase

Gene names

Name:	TPP1
Ordered Locus Names:	YMR156C
ORF Names:	YM8520.05C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dephosphorylate DNA's 3'-phosphate termini. Has a role in the repair of breaks in single stranded DNA. Ref.4
Catalytic activity	A 3'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the DNA 3' phosphatase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from direct assay. Source: SGD
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polynucleotide 3'-phosphatase activity Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Polynucleotide 3'-phosphatase		PRO_0000065580

Sequences

	Sequence		Length	Mass (Da)
	Q03796 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: ABE35B947077D687	FASTA	238	27,383
	10 20 30 40 50 60 MSHKLTILPF LIKFTPKFPQ SIDHDEHGLN VYAFDLDHTI IKPKSPNISF SRSASDWQFI 70 80 90 100 110 120 NFNSKKSTLD YLCNIIDNDP TAVIVIFSNQ GGVITVPRTS KSCTKYTNKI LLFLKAIKND 130 140 150 160 170 180 ERGETLSHRL WLYAAPKRPK TFAANHSKIT FASLGESYNN DPNIFEKVRK PMTGMVEFFK 190 200 210 220 230 RDLESAYRVS EQISPIKLNW IYYCGDAAGR KKDFSDSDIK FAENLHVEFK YPEEIFHG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Uncoupling of 3'-phosphatase and 5'-kinase functions in budding yeast. Characterization of Saccharomyces cerevisiae DNA 3'-phosphatase (TPP1)." Vance J.R., Wilson T.E. J. Biol. Chem. 276:15073-15081(2001) [PubMed: 11278831] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49705 Genomic DNA. Translation: CAA89792.1. AY557979 Genomic DNA. Translation: AAS56305.1. BK006946 Genomic DNA. Translation: DAA10051.1.
PIR	S54514.
RefSeq	NP_013877.1. NM_001182659.1.
3D structure databases
ProteinModelPortal	Q03796.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03796. 1 interaction.
STRING	Q03796.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR156C; YMR156C; YMR156C.
GeneID	855188.
KEGG	sce:YMR156C.
NMPDR	fig|4932.3.peg.4927.
Organism-specific databases
CYGD	YMR156c.
SGD	S000004765. TPP1.
Phylogenomic databases
eggNOG	fuNOG16284.
HOGENOM	HBG203453.
OMA	MRKPETG.
OrthoDB	EOG47M57H.
Gene expression databases
ArrayExpress	Q03796.
Genevestigator	Q03796.
GermOnline	YMR156C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR023214. HAD-like_dom. IPR013954. PNK3P. IPR015636. PNK_3Pase_euk. IPR006551. Polynucleotide_phosphatase. [Graphical view]
KO	K08075.
PANTHER	PTHR12083. PNK_3Pase. 1 hit.
Pfam	PF08645. PNK3P. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01664. DNA-3'-Pase. 1 hit.
ProtoNet	Search...
Other
NextBio	978653.

Entry information

Entry name

TPP1_YEAST

Accession

Primary (citable) accession number: Q03796
Secondary accession number(s): D6VZX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program