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Protein

Calpain-like protease 1

Gene

RIM13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth. Probably the protease that cleaves RIM101.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei128 – 1281By similarity
Active sitei271 – 2711By similarity
Active sitei296 – 2961By similarity

GO - Molecular functioni

GO - Biological processi

  • protein processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciYEAST:G3O-32844-MONOMER.

Protein family/group databases

MEROPSiC02.030.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-like protease 1 (EC:3.4.22.-)
Alternative name(s):
Calpain-7
Cysteine protease RIM13
Regulator of IME2 protein 13
Gene namesi
Name:RIM13
Synonyms:CPL1
Ordered Locus Names:YMR154C
ORF Names:YM8520.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR154C.
SGDiS000004763. RIM13.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi128 – 1281C → S: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Calpain-like protease 1PRO_0000207746Add
BLAST

Proteomic databases

MaxQBiQ03792.

Interactioni

Subunit structurei

Interacts with SNF7, which may act together with RIM20 as a scaffold to recruit RIM13 to its substrate RIM101.1 Publication

Protein-protein interaction databases

BioGridi35329. 182 interactions.
DIPiDIP-2809N.
IntActiQ03792. 3 interactions.
MINTiMINT-490882.

Structurei

3D structure databases

ProteinModelPortaliQ03792.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 317248Calpain catalyticAdd
BLAST

Sequence similaritiesi

Contains 1 calpain catalytic domain.Curated

Phylogenomic databases

InParanoidiQ03792.
OMAiWLLLESH.
OrthoDBiEOG092C185F.

Family and domain databases

InterProiIPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
SMARTiSM00230. CysPc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDWHEFNAA IKSIYCNAEG DSSSIINRLV GLAMKSEDST FIEAVLVLKE
60 70 80 90 100
NVSKVDKQLR FLWLTSTINS RFYPPIPISE ASPVSWNKTE YCAPGTEELQ
110 120 130 140 150
RRYPGRAKLQ NEEDYSGGIE QCRDVPDCSL VASLINLRSK NLNLPLIKQI
160 170 180 190 200
SSTKYHVNLS FNGSNKRLVT VDISQIPTSV DGKQLSLKSN DISDKIGELA
210 220 230 240 250
LLLVSKGTYS TDGSNISIDT YRLSGFLPEI TQVNSYPFEK LWKFHKSNLC
260 270 280 290 300
LMGAGTGNRS NDMIKPLVEN HDYSIIDITY DSRLVKLRDP RNSALNVEIS
310 320 330 340 350
YEQYLKNFKQ LYLNWNQEKL FKRSQVLHFR YDTSRYNKFS IVADKPLFHL
360 370 380 390 400
VNNSKVTETV WLLLESHLQD EGSQENRSVS FLNEAPECII CPIEPPVECG
410 420 430 440 450
GNHIGLQLVK LRLDAETERL LYCYSTTNNN FSIHSFSVVK EICFQRLKDT
460 470 480 490 500
KSLFAKVLFS FPYEIEGKAS FDTCNFFQNP TFELEVHSEQ DYQVLMDAAC
510 520 530 540 550
ISTSSHDLIN IQVYYFNDYE LIKPIMFDNH YQPGQGLKQD VPILTNVKYM
560 570 580 590 600
IVCSTYGPPA STEFELLASI RLSSSWRLIS GITLRSVNLI YGTYPYHCRN
610 620 630 640 650
RFHWKETSDK LKIQMTLPTK KYSTNKLFIR VVPVESSARL RMRCNIFEPE
660 670 680 690 700
SALCVYECQE YRTCPSGGIV IPDLEVSRTN IVVLMIERSV PISSCLPTEG
710 720
QLDELELFVG SSQKIRIEKY SDDVIPK
Length:727
Mass (Da):83,124
Last modified:November 1, 1997 - v1
Checksum:i315F1A542FCC40F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49705 Genomic DNA. Translation: CAA89790.1.
BK006946 Genomic DNA. Translation: DAA10049.1.
PIRiS54512.
RefSeqiNP_013875.1. NM_001182657.1.

Genome annotation databases

EnsemblFungiiYMR154C; YMR154C; YMR154C.
GeneIDi855186.
KEGGisce:YMR154C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49705 Genomic DNA. Translation: CAA89790.1.
BK006946 Genomic DNA. Translation: DAA10049.1.
PIRiS54512.
RefSeqiNP_013875.1. NM_001182657.1.

3D structure databases

ProteinModelPortaliQ03792.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35329. 182 interactions.
DIPiDIP-2809N.
IntActiQ03792. 3 interactions.
MINTiMINT-490882.

Protein family/group databases

MEROPSiC02.030.

Proteomic databases

MaxQBiQ03792.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR154C; YMR154C; YMR154C.
GeneIDi855186.
KEGGisce:YMR154C.

Organism-specific databases

EuPathDBiFungiDB:YMR154C.
SGDiS000004763. RIM13.

Phylogenomic databases

InParanoidiQ03792.
OMAiWLLLESH.
OrthoDBiEOG092C185F.

Enzyme and pathway databases

BioCyciYEAST:G3O-32844-MONOMER.

Miscellaneous databases

PROiQ03792.

Family and domain databases

InterProiIPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
SMARTiSM00230. CysPc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIM13_YEAST
AccessioniPrimary (citable) accession number: Q03792
Secondary accession number(s): D6VZX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.