Q03792 (RIM13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calpain-like protease 1
EC=3.4.22.-

Alternative name(s):
Calpain-7
Cysteine protease RIM13
Regulator of IME2 protein 13

Gene names

Name:	RIM13
Synonyms:	CPL1
Ordered Locus Names:	YMR154C
ORF Names:	YM8520.03C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth. Probably the protease that cleaves RIM101. Ref.3 Ref.4 Ref.5
Subunit structure	Interacts with SNF7, which may act together with RIM20 as a scaffold to recruit RIM13 to its substrate RIM101. Ref.6
Miscellaneous	Present with 432 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the peptidase C2 family. PalB/RIM13 subfamily. Contains 1 calpain catalytic domain.

Ontologies

Keywords
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein processing Inferred from mutant phenotype Ref.4. Source: SGD proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	calcium-dependent cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 727	727	Calpain-like protease 1		PRO_0000207746
Regions
	Domain	70 – 317	248	Calpain catalytic
Sites
	Active site	128	1	By similarity
	Active site	271	1	By similarity
	Active site	296	1	By similarity
Experimental info
	Mutagenesis	128	1	C → S: Loss of function. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q03792 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 315F1A542FCC40F3	FASTA	727	83,124
	10 20 30 40 50 60 MNDWHEFNAA IKSIYCNAEG DSSSIINRLV GLAMKSEDST FIEAVLVLKE NVSKVDKQLR 70 80 90 100 110 120 FLWLTSTINS RFYPPIPISE ASPVSWNKTE YCAPGTEELQ RRYPGRAKLQ NEEDYSGGIE 130 140 150 160 170 180 QCRDVPDCSL VASLINLRSK NLNLPLIKQI SSTKYHVNLS FNGSNKRLVT VDISQIPTSV 190 200 210 220 230 240 DGKQLSLKSN DISDKIGELA LLLVSKGTYS TDGSNISIDT YRLSGFLPEI TQVNSYPFEK 250 260 270 280 290 300 LWKFHKSNLC LMGAGTGNRS NDMIKPLVEN HDYSIIDITY DSRLVKLRDP RNSALNVEIS 310 320 330 340 350 360 YEQYLKNFKQ LYLNWNQEKL FKRSQVLHFR YDTSRYNKFS IVADKPLFHL VNNSKVTETV 370 380 390 400 410 420 WLLLESHLQD EGSQENRSVS FLNEAPECII CPIEPPVECG GNHIGLQLVK LRLDAETERL 430 440 450 460 470 480 LYCYSTTNNN FSIHSFSVVK EICFQRLKDT KSLFAKVLFS FPYEIEGKAS FDTCNFFQNP 490 500 510 520 530 540 TFELEVHSEQ DYQVLMDAAC ISTSSHDLIN IQVYYFNDYE LIKPIMFDNH YQPGQGLKQD 550 560 570 580 590 600 VPILTNVKYM IVCSTYGPPA STEFELLASI RLSSSWRLIS GITLRSVNLI YGTYPYHCRN 610 620 630 640 650 660 RFHWKETSDK LKIQMTLPTK KYSTNKLFIR VVPVESSARL RMRCNIFEPE SALCVYECQE 670 680 690 700 710 720 YRTCPSGGIV IPDLEVSRTN IVVLMIERSV PISSCLPTEG QLDELELFVG SSQKIRIEKY SDDVIPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Proteolytic activation of Rim1p, a positive regulator of yeast sporulation and invasive growth." Li W., Mitchell A.P. Genetics 145:63-73(1997) [PubMed: 9017390] [Abstract] Cited for: FUNCTION.
[4]	"The protease activity of a calpain-like cysteine protease in Saccharomyces cerevisiae is required for alkaline adaptation and sporulation." Futai E., Maeda T., Sorimachi H., Kitamoto K., Ishiura S., Suzuki K. Mol. Gen. Genet. 260:559-568(1999) [PubMed: 9928935] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF CYS-128.
[5]	"Alkaline response genes of Saccharomyces cerevisiae and their relationship to the RIM101 pathway." Lamb T.M., Xu W., Diamond A., Mitchell A.P. J. Biol. Chem. 276:1850-1856(2001) [PubMed: 11050096] [Abstract] Cited for: FUNCTION.
[6]	"A comprehensive two-hybrid analysis to explore the yeast protein interactome." Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., Sakaki Y. Proc. Natl. Acad. Sci. U.S.A. 98:4569-4574(2001) [PubMed: 11283351] [Abstract] Cited for: INTERACTION WITH SNF7.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49705 Genomic DNA. Translation: CAA89790.1. BK006946 Genomic DNA. Translation: DAA10049.1.
PIR	S54512.
RefSeq	NP_013875.1. NM_001182657.1.
3D structure databases
ProteinModelPortal	Q03792.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2809N.
IntAct	Q03792. 2 interactions.
MINT	MINT-490882.
STRING	Q03792.
Protein family/group databases
MEROPS	C02.008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR154C; YMR154C; YMR154C.
GeneID	855186.
KEGG	sce:YMR154C.
NMPDR	fig|4932.3.peg.4925.
Organism-specific databases
CYGD	YMR154c.
SGD	S000004763. RIM13.
Phylogenomic databases
eggNOG	fuNOG06730.
GeneTree	EFGT00050000001861.
OMA	TTEERIV.
OrthoDB	EOG4W3WX1.
Gene expression databases
ArrayExpress	Q03792.
Genevestigator	Q03792.
GermOnline	YMR154C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001300. Peptidase_C2_calpain_cat. [Graphical view]
SMART	SM00230. CysPc. 1 hit. [Graphical view]
PROSITE	PS50203. CALPAIN_CAT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	978647.

Entry information

Entry name

RIM13_YEAST

Accession

Primary (citable) accession number: Q03792
Secondary accession number(s): D6VZX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program