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Protein

Calpain-like protease 1

Gene

RIM13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth. Probably the protease that cleaves RIM101.3 Publications

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei128By similarity1
Active sitei271By similarity1
Active sitei296By similarity1

GO - Molecular functioni

GO - Biological processi

  • protein processing Source: SGD

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciYEAST:G3O-32844-MONOMER

Protein family/group databases

MEROPSiC02.030

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-like protease 1 (EC:3.4.22.-)
Alternative name(s):
Calpain-7
Cysteine protease RIM13
Regulator of IME2 protein 13
Gene namesi
Name:RIM13
Synonyms:CPL1
Ordered Locus Names:YMR154C
ORF Names:YM8520.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR154C
SGDiS000004763 RIM13

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi128C → S: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077461 – 727Calpain-like protease 1Add BLAST727

Proteomic databases

MaxQBiQ03792
PaxDbiQ03792
PRIDEiQ03792

Interactioni

Subunit structurei

Interacts with SNF7, which may act together with RIM20 as a scaffold to recruit RIM13 to its substrate RIM101.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CKA1P157902EBI-27327,EBI-9533

Protein-protein interaction databases

BioGridi35329, 384 interactors
DIPiDIP-2809N
IntActiQ03792, 7 interactors
MINTiQ03792
STRINGi4932.YMR154C

Structurei

3D structure databases

ProteinModelPortaliQ03792
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini70 – 317Calpain catalyticAdd BLAST248

Sequence similaritiesi

Phylogenomic databases

InParanoidiQ03792
OMAiQPIQINE
OrthoDBiEOG092C185F

Family and domain databases

InterProiView protein in InterPro
IPR001300 Peptidase_C2_calpain_cat
SMARTiView protein in SMART
SM00230 CysPc, 1 hit

Sequencei

Sequence statusi: Complete.

Q03792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDWHEFNAA IKSIYCNAEG DSSSIINRLV GLAMKSEDST FIEAVLVLKE
60 70 80 90 100
NVSKVDKQLR FLWLTSTINS RFYPPIPISE ASPVSWNKTE YCAPGTEELQ
110 120 130 140 150
RRYPGRAKLQ NEEDYSGGIE QCRDVPDCSL VASLINLRSK NLNLPLIKQI
160 170 180 190 200
SSTKYHVNLS FNGSNKRLVT VDISQIPTSV DGKQLSLKSN DISDKIGELA
210 220 230 240 250
LLLVSKGTYS TDGSNISIDT YRLSGFLPEI TQVNSYPFEK LWKFHKSNLC
260 270 280 290 300
LMGAGTGNRS NDMIKPLVEN HDYSIIDITY DSRLVKLRDP RNSALNVEIS
310 320 330 340 350
YEQYLKNFKQ LYLNWNQEKL FKRSQVLHFR YDTSRYNKFS IVADKPLFHL
360 370 380 390 400
VNNSKVTETV WLLLESHLQD EGSQENRSVS FLNEAPECII CPIEPPVECG
410 420 430 440 450
GNHIGLQLVK LRLDAETERL LYCYSTTNNN FSIHSFSVVK EICFQRLKDT
460 470 480 490 500
KSLFAKVLFS FPYEIEGKAS FDTCNFFQNP TFELEVHSEQ DYQVLMDAAC
510 520 530 540 550
ISTSSHDLIN IQVYYFNDYE LIKPIMFDNH YQPGQGLKQD VPILTNVKYM
560 570 580 590 600
IVCSTYGPPA STEFELLASI RLSSSWRLIS GITLRSVNLI YGTYPYHCRN
610 620 630 640 650
RFHWKETSDK LKIQMTLPTK KYSTNKLFIR VVPVESSARL RMRCNIFEPE
660 670 680 690 700
SALCVYECQE YRTCPSGGIV IPDLEVSRTN IVVLMIERSV PISSCLPTEG
710 720
QLDELELFVG SSQKIRIEKY SDDVIPK
Length:727
Mass (Da):83,124
Last modified:November 1, 1997 - v1
Checksum:i315F1A542FCC40F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49705 Genomic DNA Translation: CAA89790.1
BK006946 Genomic DNA Translation: DAA10049.1
PIRiS54512
RefSeqiNP_013875.1, NM_001182657.1

Genome annotation databases

EnsemblFungiiYMR154C; YMR154C; YMR154C
GeneIDi855186
KEGGisce:YMR154C

Similar proteinsi

Entry informationi

Entry nameiRIM13_YEAST
AccessioniPrimary (citable) accession number: Q03792
Secondary accession number(s): D6VZX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health