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Protein

Trafficking protein particle complex subunit 23

Gene

TRS23

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation.3 Publications

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
  2. chromosome organization Source: SGD
  3. ER to Golgi vesicle-mediated transport Source: SGD
  4. positive regulation of Rab GTPase activity Source: GOC
Complete GO annotation...

Keywords - Biological processi

Autophagy, ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29819-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking protein particle complex subunit 23
Short name:
TRAPP subunit 23
Alternative name(s):
Transport protein particle 23 kDa subunit
Gene namesi
Name:TRS23
Ordered Locus Names:YDR246W
ORF Names:YD8419.13
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR246w.
SGDiS000002654. TRS23.

Subcellular locationi

GO - Cellular componenti

  1. cis-Golgi network Source: InterPro
  2. endoplasmic reticulum Source: UniProtKB-SubCell
  3. pre-autophagosomal structure Source: UniProtKB-SubCell
  4. TRAPPIII protein complex Source: SGD
  5. TRAPPII protein complex Source: SGD
  6. TRAPPI protein complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 219219Trafficking protein particle complex subunit 23PRO_0000211571Add
BLAST

Proteomic databases

MaxQBiQ03784.
PaxDbiQ03784.

Expressioni

Gene expression databases

GenevestigatoriQ03784.

Interactioni

Subunit structurei

Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BET3P3614910EBI-19474,EBI-3567

Protein-protein interaction databases

BioGridi32297. 18 interactions.
DIPiDIP-5813N.
IntActiQ03784. 4 interactions.
MINTiMINT-640620.
STRINGi4932.YDR246W.

Structurei

Secondary structure

1
219
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97
Beta strandi17 – 204
Helixi30 – 4011
Helixi44 – 496
Beta strandi121 – 1266
Beta strandi128 – 1336
Beta strandi139 – 1468
Helixi170 – 19122
Helixi205 – 21814

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUEX-ray3.70A/G/M/S1-219[»]
SMRiQ03784. Positions 2-219.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03784.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5122.
GeneTreeiENSGT00510000047061.
HOGENOMiHOG000165619.
InParanoidiQ03784.
OMAiNEYLILA.
OrthoDBiEOG7M98VC.

Family and domain databases

InterProiIPR011012. Longin-like_dom.
IPR007233. Sybindin.
[Graphical view]
PANTHERiPTHR23249. PTHR23249. 1 hit.
PfamiPF04099. Sybindin. 3 hits.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 2 hits.

Sequencei

Sequence statusi: Complete.

Q03784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIETILVIN KSGGLIYQRN FTNDEQKLNS NEYLILASTL HGVFAIASQL
60 70 80 90 100
TPKALQLTQQ TNIENTIPYI PYVGMSSNRS DTRNGGGNNN KHTNNEKLGS
110 120 130 140 150
FKGDDFFKEP FTNWNKSGLR QLCTDQFTMF IYQTLTGLKF VAISSSVMPQ
160 170 180 190 200
RQPTIATTDK PDRPKSTSNL AIQIADNFLR KVYCLYSDYV MKDPSYSMEM
210
PIRSNLFDEK VKKMVENLQ
Length:219
Mass (Da):24,863
Last modified:October 31, 1996 - v1
Checksum:i8B32B1D5FE3846ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49701 Genomic DNA. Translation: CAA89732.1.
BK006938 Genomic DNA. Translation: DAA12086.1.
PIRiS54542.
RefSeqiNP_010532.3. NM_001180554.3.

Genome annotation databases

EnsemblFungiiYDR246W; YDR246W; YDR246W.
GeneIDi851833.
KEGGisce:YDR246W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49701 Genomic DNA. Translation: CAA89732.1.
BK006938 Genomic DNA. Translation: DAA12086.1.
PIRiS54542.
RefSeqiNP_010532.3. NM_001180554.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUEX-ray3.70A/G/M/S1-219[»]
SMRiQ03784. Positions 2-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32297. 18 interactions.
DIPiDIP-5813N.
IntActiQ03784. 4 interactions.
MINTiMINT-640620.
STRINGi4932.YDR246W.

Proteomic databases

MaxQBiQ03784.
PaxDbiQ03784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR246W; YDR246W; YDR246W.
GeneIDi851833.
KEGGisce:YDR246W.

Organism-specific databases

CYGDiYDR246w.
SGDiS000002654. TRS23.

Phylogenomic databases

eggNOGiCOG5122.
GeneTreeiENSGT00510000047061.
HOGENOMiHOG000165619.
InParanoidiQ03784.
OMAiNEYLILA.
OrthoDBiEOG7M98VC.

Enzyme and pathway databases

BioCyciYEAST:G3O-29819-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ03784.
NextBioi969724.
PROiQ03784.

Gene expression databases

GenevestigatoriQ03784.

Family and domain databases

InterProiIPR011012. Longin-like_dom.
IPR007233. Sybindin.
[Graphical view]
PANTHERiPTHR23249. PTHR23249. 1 hit.
PfamiPF04099. Sybindin. 3 hits.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
    Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
    EMBO J. 17:2494-2503(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
  4. "TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
    Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
    Mol. Cell 7:433-442(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
  5. Cited for: IDENTIFICATION IN TRAPP COMPLEX.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
    Yip C.K., Berscheminski J., Walz T.
    Nat. Struct. Mol. Biol. 17:1298-1304(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
  8. Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.

Entry informationi

Entry nameiTRS23_YEAST
AccessioniPrimary (citable) accession number: Q03784
Secondary accession number(s): D6VSM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 15, 2002
Last sequence update: October 31, 1996
Last modified: March 31, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.