Q03784 (TRS23_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trafficking protein particle complex subunit 23
Short name=TRAPP subunit 23

Alternative name(s):
Transport protein particle 23 kDa subunit

Gene names

Name:	TRS23
Ordered Locus Names:	YDR246W
ORF Names:	YD8419.13

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Ref.4 Ref.7 Ref.8
Subunit structure	Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85. Ref.3 Ref.4 Ref.5 Ref.7 Ref.8
Subcellular location	Golgi apparatus › cis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure Ref.4 Ref.8.
Miscellaneous	Present with 3130 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.

Ontologies

Keywords
Biological process	Autophagy ER-Golgi transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.4. Source: SGD autophagy Inferred from electronic annotation. Source: UniProtKB-KW chromosome organization Inferred from mutant phenotype PubMed 18439903. Source: SGD
Cellular_component	TRAPP complex Inferred from direct assay PubMed 10727015Ref.3. Source: SGD endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell pre-autophagosomal structure Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BET3	P36149	10	EBI-19474,EBI-3567

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 219

219

Trafficking protein particle complex subunit 23

PRO_0000211571

Secondary structure

1

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219

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Beta strand

17 – 20

4

Helix

30 – 40

11

Helix

44 – 49

6

Beta strand

121 – 126

6

Beta strand

128 – 133

6

Beta strand

139 – 146

8

Helix

170 – 191

22

Helix

205 – 218

14

Sequences

	Sequence		Length	Mass (Da)
	Q03784 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8B32B1D5FE3846ED	FASTA	219	24,863
	10 20 30 40 50 60 MAIETILVIN KSGGLIYQRN FTNDEQKLNS NEYLILASTL HGVFAIASQL TPKALQLTQQ 70 80 90 100 110 120 TNIENTIPYI PYVGMSSNRS DTRNGGGNNN KHTNNEKLGS FKGDDFFKEP FTNWNKSGLR 130 140 150 160 170 180 QLCTDQFTMF IYQTLTGLKF VAISSSVMPQ RQPTIATTDK PDRPKSTSNL AIQIADNFLR 190 200 210 KVYCLYSDYV MKDPSYSMEM PIRSNLFDEK VKKMVENLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion." Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S. EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
[4]	"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport." Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S. Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
[5]	"Functional organization of the yeast proteome by systematic analysis of protein complexes." Gavin A.-C., Boesche M., Krause R., Grandi P., Marzioch M., Bauer A., Schultz J., Rick J.M., Michon A.-M., Cruciat C.-M., Remor M., Hoefert C., Schelder M., Brajenovic M., Ruffner H., Merino A., Klein K., Hudak M. , Dickson D., Rudi T., Gnau V., Bauch A., Bastuck S., Huhse B., Leutwein C., Heurtier M.-A., Copley R.R., Edelmann A., Querfurth E., Rybin V., Drewes G., Raida M., Bouwmeester T., Bork P., Seraphin B., Kuster B., Neubauer G., Superti-Furga G. Nature 415:141-147(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN TRAPP COMPLEX.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Molecular architecture of the TRAPPII complex and implications for vesicle tethering." Yip C.K., Berscheminski J., Walz T. Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
[8]	"Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy." Lynch-Day M.A., Bhandari D., Menon S., Huang J., Cai H., Bartholomew C.R., Brumell J.H., Ferro-Novick S., Klionsky D.J. Proc. Natl. Acad. Sci. U.S.A. 107:7811-7816(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z49701 Genomic DNA. Translation: CAA89732.1.
BK006938 Genomic DNA. Translation: DAA12086.1.

PIR

S54542.

RefSeq

NP_010532.3. NM_001180554.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CUE	X-ray	3.70	A/G/M/S	1-219	[»]

ProteinModelPortal

Q03784.

SMR

Q03784. Positions 2-219.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5813N.

IntAct

Q03784. 2 interactions.

MINT

MINT-640620.

STRING

4932.YDR246W.

Proteomic databases

PaxDb

Q03784.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDR246W; YDR246W; YDR246W.

GeneID

851833.

KEGG

sce:YDR246W.
sce:YDR249C.

Organism-specific databases

CYGD

YDR246w.

SGD

S000002654. TRS23.

Phylogenomic databases

eggNOG

COG5122.

GeneTree

ENSGT00510000047061.

HOGENOM

HOG000165619.

OMA

YLILAST.

OrthoDB

EOG4P2TBS.

Gene expression databases

Genevestigator

Q03784.

GermOnline

YDR246W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR011012. Longin-like_dom.
IPR007233. Sybindin.
[Graphical view]

PANTHER

PTHR23249. PTHR23249. 1 hit.

Pfam

PF04099. Sybindin. 3 hits.
[Graphical view]

SUPFAM

SSF64356. Longin_like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q03784.

NextBio

969724.

Entry information

Entry name

TRS23_YEAST

Accession

Primary (citable) accession number: Q03784
Secondary accession number(s): D6VSM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program