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Q03784 (TRS23_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trafficking protein particle complex subunit 23

Short name=TRAPP subunit 23
Alternative name(s):
Transport protein particle 23 kDa subunit
Gene names
Name:TRS23
Ordered Locus Names:YDR246W
ORF Names:YD8419.13
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Ref.4 Ref.7 Ref.8

Subunit structure

Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85. Ref.3 Ref.4 Ref.5 Ref.7 Ref.8

Subcellular location

Golgi apparatuscis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure Ref.4 Ref.8.

Miscellaneous

Present with 3130 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BET3P3614910EBI-19474,EBI-3567

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219Trafficking protein particle complex subunit 23
PRO_0000211571

Secondary structure

................... 219
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q03784 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8B32B1D5FE3846ED

FASTA21924,863
        10         20         30         40         50         60 
MAIETILVIN KSGGLIYQRN FTNDEQKLNS NEYLILASTL HGVFAIASQL TPKALQLTQQ 

        70         80         90        100        110        120 
TNIENTIPYI PYVGMSSNRS DTRNGGGNNN KHTNNEKLGS FKGDDFFKEP FTNWNKSGLR 

       130        140        150        160        170        180 
QLCTDQFTMF IYQTLTGLKF VAISSSVMPQ RQPTIATTDK PDRPKSTSNL AIQIADNFLR 

       190        200        210 
KVYCLYSDYV MKDPSYSMEM PIRSNLFDEK VKKMVENLQ 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
[4]"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
[5]"Functional organization of the yeast proteome by systematic analysis of protein complexes."
Gavin A.-C., Boesche M., Krause R., Grandi P., Marzioch M., Bauer A., Schultz J., Rick J.M., Michon A.-M., Cruciat C.-M., Remor M., Hoefert C., Schelder M., Brajenovic M., Ruffner H., Merino A., Klein K., Hudak M. expand/collapse author list , Dickson D., Rudi T., Gnau V., Bauch A., Bastuck S., Huhse B., Leutwein C., Heurtier M.-A., Copley R.R., Edelmann A., Querfurth E., Rybin V., Drewes G., Raida M., Bouwmeester T., Bork P., Seraphin B., Kuster B., Neubauer G., Superti-Furga G.
Nature 415:141-147(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN TRAPP COMPLEX.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
Yip C.K., Berscheminski J., Walz T.
Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
[8]"Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy."
Lynch-Day M.A., Bhandari D., Menon S., Huang J., Cai H., Bartholomew C.R., Brumell J.H., Ferro-Novick S., Klionsky D.J.
Proc. Natl. Acad. Sci. U.S.A. 107:7811-7816(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49701 Genomic DNA. Translation: CAA89732.1.
BK006938 Genomic DNA. Translation: DAA12086.1.
PIRS54542.
RefSeqNP_010532.3. NM_001180554.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUEX-ray3.70A/G/M/S1-219[»]
ProteinModelPortalQ03784.
SMRQ03784. Positions 2-219.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32297. 17 interactions.
DIPDIP-5813N.
IntActQ03784. 4 interactions.
MINTMINT-640620.
STRING4932.YDR246W.

Proteomic databases

MaxQBQ03784.
PaxDbQ03784.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR246W; YDR246W; YDR246W.
GeneID851833.
KEGGsce:YDR246W.

Organism-specific databases

CYGDYDR246w.
SGDS000002654. TRS23.

Phylogenomic databases

eggNOGCOG5122.
GeneTreeENSGT00510000047061.
HOGENOMHOG000165619.
OMAYLILAST.
OrthoDBEOG7M98VC.

Enzyme and pathway databases

BioCycYEAST:G3O-29819-MONOMER.

Gene expression databases

GenevestigatorQ03784.

Family and domain databases

InterProIPR011012. Longin-like_dom.
IPR007233. Sybindin.
[Graphical view]
PANTHERPTHR23249. PTHR23249. 1 hit.
PfamPF04099. Sybindin. 3 hits.
[Graphical view]
SUPFAMSSF64356. SSF64356. 2 hits.
ProtoNetSearch...

Other

EvolutionaryTraceQ03784.
NextBio969724.
PROQ03784.

Entry information

Entry nameTRS23_YEAST
AccessionPrimary (citable) accession number: Q03784
Secondary accession number(s): D6VSM6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references