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Q03778

- RIFK_YEAST

UniProt

Q03778 - RIFK_YEAST

Protein

Riboflavin kinase

Gene

FMN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.

    Catalytic activityi

    ATP + riboflavin = ADP + FMN.

    Cofactori

    Zinc or magnesium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi72 – 721Magnesium or zincBy similarity
    Active sitei155 – 1551NucleophileBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. riboflavin kinase activity Source: SGD

    GO - Biological processi

    1. FMN biosynthetic process Source: SGD
    2. riboflavin biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:YDR236C-MONOMER.
    YEAST:YDR236C-MONOMER.
    UniPathwayiUPA00276; UER00406.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Riboflavin kinase (EC:2.7.1.26)
    Alternative name(s):
    Flavin mononucleotide kinase 1
    Gene namesi
    Name:FMN1
    Ordered Locus Names:YDR236C
    ORF Names:YD8419.03C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR236c.
    SGDiS000002644. FMN1.

    Subcellular locationi

    Microsome 1 Publication. Mitochondrion inner membrane 1 Publication. Endoplasmic reticulum Curated
    Note: 90% microsomal. 10% mitochondrial. Found to be present in the inner membrane of mitochondria. The C-terminus is located in the matrix.

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: SGD
    3. mitochondrial inner membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 218199Riboflavin kinasePRO_0000030437Add
    BLAST

    Proteomic databases

    MaxQBiQ03778.
    PaxDbiQ03778.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03778.

    Interactioni

    Protein-protein interaction databases

    BioGridi32287. 11 interactions.
    DIPiDIP-1316N.
    IntActiQ03778. 1 interaction.
    MINTiMINT-406034.
    STRINGi4932.YDR236C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03778.
    SMRiQ03778. Positions 57-216.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavokinase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0196.
    GeneTreeiENSGT00390000015537.
    HOGENOMiHOG000260803.
    KOiK00861.
    OMAiCDIVCGF.
    OrthoDBiEOG7K3V05.

    Family and domain databases

    Gene3Di2.40.30.30. 1 hit.
    InterProiIPR023468. Riboflavin_kinase.
    IPR015865. Riboflavin_kinase_bac/euk.
    IPR023465. Riboflavin_kinase_domain.
    [Graphical view]
    PANTHERiPTHR22749. PTHR22749. 1 hit.
    PfamiPF01687. Flavokinase. 1 hit.
    [Graphical view]
    SMARTiSM00904. Flavokinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF82114. SSF82114. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03778-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFTWTIYVSL LLVLAGTFLM NRNTNTDIID TFKREVDLPI PAQPGPPFPL    50
    VTDYCDIVCG FGRGSAELGI PTANVPINQL PKGINDLDLG VYFGFAHIKT 100
    VDGQELSVET RRDGRTVVYN YGQYLSEAND DLSVLPMVLS VGKNPFYGND 150
    FKTMELHIIH DFKNDFYGAR VKFNILGHIR PELNYTTKEA LIEDINIDIR 200
    TAQTVLATPP YQVFKQQL 218
    Length:218
    Mass (Da):24,537
    Last modified:November 1, 1996 - v1
    Checksum:iD96995F4F2EBC9F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49701 Genomic DNA. Translation: CAA89722.1.
    AY557733 Genomic DNA. Translation: AAS56059.1.
    BK006938 Genomic DNA. Translation: DAA12077.1.
    PIRiS54532.
    RefSeqiNP_010522.1. NM_001180544.1.

    Genome annotation databases

    EnsemblFungiiYDR236C; YDR236C; YDR236C.
    GeneIDi851822.
    KEGGisce:YDR236C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49701 Genomic DNA. Translation: CAA89722.1 .
    AY557733 Genomic DNA. Translation: AAS56059.1 .
    BK006938 Genomic DNA. Translation: DAA12077.1 .
    PIRi S54532.
    RefSeqi NP_010522.1. NM_001180544.1.

    3D structure databases

    ProteinModelPortali Q03778.
    SMRi Q03778. Positions 57-216.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32287. 11 interactions.
    DIPi DIP-1316N.
    IntActi Q03778. 1 interaction.
    MINTi MINT-406034.
    STRINGi 4932.YDR236C.

    Proteomic databases

    MaxQBi Q03778.
    PaxDbi Q03778.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR236C ; YDR236C ; YDR236C .
    GeneIDi 851822.
    KEGGi sce:YDR236C.

    Organism-specific databases

    CYGDi YDR236c.
    SGDi S000002644. FMN1.

    Phylogenomic databases

    eggNOGi COG0196.
    GeneTreei ENSGT00390000015537.
    HOGENOMi HOG000260803.
    KOi K00861.
    OMAi CDIVCGF.
    OrthoDBi EOG7K3V05.

    Enzyme and pathway databases

    UniPathwayi UPA00276 ; UER00406 .
    BioCyci MetaCyc:YDR236C-MONOMER.
    YEAST:YDR236C-MONOMER.

    Miscellaneous databases

    NextBioi 969695.
    PROi Q03778.

    Gene expression databases

    Genevestigatori Q03778.

    Family and domain databases

    Gene3Di 2.40.30.30. 1 hit.
    InterProi IPR023468. Riboflavin_kinase.
    IPR015865. Riboflavin_kinase_bac/euk.
    IPR023465. Riboflavin_kinase_domain.
    [Graphical view ]
    PANTHERi PTHR22749. PTHR22749. 1 hit.
    Pfami PF01687. Flavokinase. 1 hit.
    [Graphical view ]
    SMARTi SM00904. Flavokinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF82114. SSF82114. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Molecular characterization of FMN1, the structural gene for the monofunctional flavokinase of Saccharomyces cerevisiae."
      Santos M.A., Jimenez A., Revuelta J.L.
      J. Biol. Chem. 275:28618-28624(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRIFK_YEAST
    AccessioniPrimary (citable) accession number: Q03778
    Secondary accession number(s): D6VSL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    FMN1 is essential for growth on rich medium.
    Present with 830 molecules/cell in log phase SD medium.1 Publication

    Caution

    The subcellular location of this enzyme needs further confirmation.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3