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Q03778 (RIFK_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Riboflavin kinase

EC=2.7.1.26
Alternative name(s):
Flavin mononucleotide kinase 1
Gene names
Name:FMN1
Ordered Locus Names:YDR236C
ORF Names:YD8419.03C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.

Catalytic activity

ATP + riboflavin = ADP + FMN.

Cofactor

Zinc or magnesium By similarity.

Pathway

Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.

Subcellular location

Microsome. Mitochondrion inner membrane. Endoplasmic reticulum Potential. Note: 90% microsomal. 10% mitochondrial. Found to be present in the inner membrane of mitochondria. The C-terminus is located in the matrix. Ref.4

Miscellaneous

FMN1 is essential for growth on rich medium.

Present with 830 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the flavokinase family.

Caution

The subcellular location of this enzyme needs further confirmation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 218199Riboflavin kinase
PRO_0000030437

Sites

Active site1551Nucleophile By similarity
Metal binding721Magnesium or zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03778 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D96995F4F2EBC9F4

FASTA21824,537
        10         20         30         40         50         60 
MFTWTIYVSL LLVLAGTFLM NRNTNTDIID TFKREVDLPI PAQPGPPFPL VTDYCDIVCG 

        70         80         90        100        110        120 
FGRGSAELGI PTANVPINQL PKGINDLDLG VYFGFAHIKT VDGQELSVET RRDGRTVVYN 

       130        140        150        160        170        180 
YGQYLSEAND DLSVLPMVLS VGKNPFYGND FKTMELHIIH DFKNDFYGAR VKFNILGHIR 

       190        200        210 
PELNYTTKEA LIEDINIDIR TAQTVLATPP YQVFKQQL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Molecular characterization of FMN1, the structural gene for the monofunctional flavokinase of Saccharomyces cerevisiae."
Santos M.A., Jimenez A., Revuelta J.L.
J. Biol. Chem. 275:28618-28624(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49701 Genomic DNA. Translation: CAA89722.1.
AY557733 Genomic DNA. Translation: AAS56059.1.
BK006938 Genomic DNA. Translation: DAA12077.1.
PIRS54532.
RefSeqNP_010522.1. NM_001180544.1.

3D structure databases

ProteinModelPortalQ03778.
SMRQ03778. Positions 57-216.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32287. 11 interactions.
DIPDIP-1316N.
IntActQ03778. 1 interaction.
MINTMINT-406034.
STRING4932.YDR236C.

Proteomic databases

MaxQBQ03778.
PaxDbQ03778.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR236C; YDR236C; YDR236C.
GeneID851822.
KEGGsce:YDR236C.

Organism-specific databases

CYGDYDR236c.
SGDS000002644. FMN1.

Phylogenomic databases

eggNOGCOG0196.
GeneTreeENSGT00390000015537.
HOGENOMHOG000260803.
KOK00861.
OMACDIVCGF.
OrthoDBEOG7K3V05.

Enzyme and pathway databases

BioCycMetaCyc:YDR236C-MONOMER.
YEAST:YDR236C-MONOMER.
UniPathwayUPA00276; UER00406.

Gene expression databases

GenevestigatorQ03778.

Family and domain databases

Gene3D2.40.30.30. 1 hit.
InterProIPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view]
PANTHERPTHR22749. PTHR22749. 1 hit.
PfamPF01687. Flavokinase. 1 hit.
[Graphical view]
SMARTSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMSSF82114. SSF82114. 2 hits.
ProtoNetSearch...

Other

NextBio969695.
PROQ03778.

Entry information

Entry nameRIFK_YEAST
AccessionPrimary (citable) accession number: Q03778
Secondary accession number(s): D6VSL7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways