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Protein

Riboflavin kinase

Gene

FMN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.

Catalytic activityi

ATP + riboflavin = ADP + FMN.

Cofactori

Zn2+By similarity, Mg2+By similarityNote: Zinc or magnesium.By similarity

Pathwayi: FMN biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FMN from riboflavin (ATP route).
Proteins known to be involved in this subpathway in this organism are:
  1. Riboflavin kinase (FMN1)
This subpathway is part of the pathway FMN biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FMN from riboflavin (ATP route), the pathway FMN biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi72Magnesium or zincBy similarity1
Active sitei155NucleophileBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • riboflavin kinase activity Source: SGD

GO - Biological processi

  • FMN biosynthetic process Source: SGD
  • riboflavin biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YDR236C-MONOMER.
YEAST:YDR236C-MONOMER.
ReactomeiR-SCE-196843. Vitamin B2 (riboflavin) metabolism.
UniPathwayiUPA00276; UER00406.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin kinase (EC:2.7.1.26)
Alternative name(s):
Flavin mononucleotide kinase 1
Gene namesi
Name:FMN1
Ordered Locus Names:YDR236C
ORF Names:YD8419.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR236C.
SGDiS000002644. FMN1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: SGD
  • mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000003043720 – 218Riboflavin kinaseAdd BLAST199

Proteomic databases

MaxQBiQ03778.
PRIDEiQ03778.

PTM databases

iPTMnetiQ03778.

Interactioni

Protein-protein interaction databases

BioGridi32287. 11 interactors.
DIPiDIP-1316N.
IntActiQ03778. 1 interactor.
MINTiMINT-406034.

Structurei

3D structure databases

ProteinModelPortaliQ03778.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the flavokinase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000015537.
HOGENOMiHOG000260803.
InParanoidiQ03778.
KOiK00861.
OMAiPFYKNER.
OrthoDBiEOG092C5F1Q.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
InterProiIPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 2 hits.
PfamiPF01687. Flavokinase. 1 hit.
[Graphical view]
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03778-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTWTIYVSL LLVLAGTFLM NRNTNTDIID TFKREVDLPI PAQPGPPFPL
60 70 80 90 100
VTDYCDIVCG FGRGSAELGI PTANVPINQL PKGINDLDLG VYFGFAHIKT
110 120 130 140 150
VDGQELSVET RRDGRTVVYN YGQYLSEAND DLSVLPMVLS VGKNPFYGND
160 170 180 190 200
FKTMELHIIH DFKNDFYGAR VKFNILGHIR PELNYTTKEA LIEDINIDIR
210
TAQTVLATPP YQVFKQQL
Length:218
Mass (Da):24,537
Last modified:November 1, 1996 - v1
Checksum:iD96995F4F2EBC9F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49701 Genomic DNA. Translation: CAA89722.1.
AY557733 Genomic DNA. Translation: AAS56059.1.
BK006938 Genomic DNA. Translation: DAA12077.1.
PIRiS54532.
RefSeqiNP_010522.1. NM_001180544.1.

Genome annotation databases

EnsemblFungiiYDR236C; YDR236C; YDR236C.
GeneIDi851822.
KEGGisce:YDR236C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49701 Genomic DNA. Translation: CAA89722.1.
AY557733 Genomic DNA. Translation: AAS56059.1.
BK006938 Genomic DNA. Translation: DAA12077.1.
PIRiS54532.
RefSeqiNP_010522.1. NM_001180544.1.

3D structure databases

ProteinModelPortaliQ03778.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32287. 11 interactors.
DIPiDIP-1316N.
IntActiQ03778. 1 interactor.
MINTiMINT-406034.

PTM databases

iPTMnetiQ03778.

Proteomic databases

MaxQBiQ03778.
PRIDEiQ03778.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR236C; YDR236C; YDR236C.
GeneIDi851822.
KEGGisce:YDR236C.

Organism-specific databases

EuPathDBiFungiDB:YDR236C.
SGDiS000002644. FMN1.

Phylogenomic databases

GeneTreeiENSGT00390000015537.
HOGENOMiHOG000260803.
InParanoidiQ03778.
KOiK00861.
OMAiPFYKNER.
OrthoDBiEOG092C5F1Q.

Enzyme and pathway databases

UniPathwayiUPA00276; UER00406.
BioCyciMetaCyc:YDR236C-MONOMER.
YEAST:YDR236C-MONOMER.
ReactomeiR-SCE-196843. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

PROiQ03778.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
InterProiIPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 2 hits.
PfamiPF01687. Flavokinase. 1 hit.
[Graphical view]
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiRIFK_YEAST
AccessioniPrimary (citable) accession number: Q03778
Secondary accession number(s): D6VSL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

FMN1 is essential for growth on rich medium.
Present with 830 molecules/cell in log phase SD medium.1 Publication

Caution

The subcellular location of this enzyme needs further confirmation.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.