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Protein

Riboflavin kinase

Gene

FMN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.

Miscellaneous

FMN1 is essential for growth on rich medium.
Present with 830 molecules/cell in log phase SD medium.1 Publication

Caution

The subcellular location of this enzyme needs further confirmation.Curated

Catalytic activityi

ATP + riboflavin = ADP + FMN.

Cofactori

Zn2+By similarity, Mg2+By similarityNote: Zinc or magnesium.By similarity

Pathwayi: FMN biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FMN from riboflavin (ATP route).
Proteins known to be involved in this subpathway in this organism are:
  1. Riboflavin kinase (FMN1)
This subpathway is part of the pathway FMN biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FMN from riboflavin (ATP route), the pathway FMN biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi72Magnesium or zincBy similarity1
Active sitei155NucleophileBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • riboflavin kinase activity Source: SGD

GO - Biological processi

  • FMN biosynthetic process Source: SGD
  • riboflavin biosynthetic process Source: InterPro

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YDR236C-MONOMER
YEAST:YDR236C-MONOMER
ReactomeiR-SCE-196843 Vitamin B2 (riboflavin) metabolism
UniPathwayiUPA00276; UER00406

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin kinase (EC:2.7.1.26)
Alternative name(s):
Flavin mononucleotide kinase 1
Gene namesi
Name:FMN1
Ordered Locus Names:YDR236C
ORF Names:YD8419.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR236C
SGDiS000002644 FMN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000003043720 – 218Riboflavin kinaseAdd BLAST199

Proteomic databases

MaxQBiQ03778
PaxDbiQ03778
PRIDEiQ03778

PTM databases

iPTMnetiQ03778

Interactioni

Protein-protein interaction databases

BioGridi32287, 188 interactors
DIPiDIP-1316N
IntActiQ03778, 2 interactors
MINTiQ03778
STRINGi4932.YDR236C

Structurei

3D structure databases

ProteinModelPortaliQ03778
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the flavokinase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000015537
HOGENOMiHOG000260803
InParanoidiQ03778
KOiK00861
OMAiSIEIHIM
OrthoDBiEOG092C5F1Q

Family and domain databases

Gene3Di2.40.30.30, 1 hit
InterProiView protein in InterPro
IPR023468 Riboflavin_kinase
IPR015865 Riboflavin_kinase_bac/euk
IPR023465 Riboflavin_kinase_dom_sf
PANTHERiPTHR22749 PTHR22749, 1 hit
PfamiView protein in Pfam
PF01687 Flavokinase, 1 hit
SMARTiView protein in SMART
SM00904 Flavokinase, 1 hit
SUPFAMiSSF82114 SSF82114, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03778-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTWTIYVSL LLVLAGTFLM NRNTNTDIID TFKREVDLPI PAQPGPPFPL
60 70 80 90 100
VTDYCDIVCG FGRGSAELGI PTANVPINQL PKGINDLDLG VYFGFAHIKT
110 120 130 140 150
VDGQELSVET RRDGRTVVYN YGQYLSEAND DLSVLPMVLS VGKNPFYGND
160 170 180 190 200
FKTMELHIIH DFKNDFYGAR VKFNILGHIR PELNYTTKEA LIEDINIDIR
210
TAQTVLATPP YQVFKQQL
Length:218
Mass (Da):24,537
Last modified:November 1, 1996 - v1
Checksum:iD96995F4F2EBC9F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49701 Genomic DNA Translation: CAA89722.1
AY557733 Genomic DNA Translation: AAS56059.1
BK006938 Genomic DNA Translation: DAA12077.1
PIRiS54532
RefSeqiNP_010522.1, NM_001180544.1

Genome annotation databases

EnsemblFungiiYDR236C; YDR236C; YDR236C
GeneIDi851822
KEGGisce:YDR236C

Similar proteinsi

Entry informationi

Entry nameiRIFK_YEAST
AccessioniPrimary (citable) accession number: Q03778
Secondary accession number(s): D6VSL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health