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Q03778

- RIFK_YEAST

UniProt

Q03778 - RIFK_YEAST

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Protein
Riboflavin kinase
Gene
FMN1, YDR236C, YD8419.03C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.

Catalytic activityi

ATP + riboflavin = ADP + FMN.

Cofactori

Zinc or magnesium By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi72 – 721Magnesium or zinc By similarity
Active sitei155 – 1551Nucleophile By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. riboflavin kinase activity Source: SGD

GO - Biological processi

  1. FMN biosynthetic process Source: SGD
  2. riboflavin biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YDR236C-MONOMER.
YEAST:YDR236C-MONOMER.
UniPathwayiUPA00276; UER00406.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin kinase (EC:2.7.1.26)
Alternative name(s):
Flavin mononucleotide kinase 1
Gene namesi
Name:FMN1
Ordered Locus Names:YDR236C
ORF Names:YD8419.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR236c.
SGDiS000002644. FMN1.

Subcellular locationi

Microsome. Mitochondrion inner membrane. Endoplasmic reticulum Reviewed prediction
Note: 90% microsomal. 10% mitochondrial. Found to be present in the inner membrane of mitochondria. The C-terminus is located in the matrix.1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-SubCell
  2. intracellular membrane-bounded organelle Source: SGD
  3. mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 218199Riboflavin kinase
PRO_0000030437Add
BLAST

Proteomic databases

MaxQBiQ03778.
PaxDbiQ03778.

Expressioni

Gene expression databases

GenevestigatoriQ03778.

Interactioni

Protein-protein interaction databases

BioGridi32287. 11 interactions.
DIPiDIP-1316N.
IntActiQ03778. 1 interaction.
MINTiMINT-406034.
STRINGi4932.YDR236C.

Structurei

3D structure databases

ProteinModelPortaliQ03778.
SMRiQ03778. Positions 57-216.

Family & Domainsi

Sequence similaritiesi

Belongs to the flavokinase family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0196.
GeneTreeiENSGT00390000015537.
HOGENOMiHOG000260803.
KOiK00861.
OMAiCDIVCGF.
OrthoDBiEOG7K3V05.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
InterProiIPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 1 hit.
PfamiPF01687. Flavokinase. 1 hit.
[Graphical view]
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03778-1 [UniParc]FASTAAdd to Basket

« Hide

MFTWTIYVSL LLVLAGTFLM NRNTNTDIID TFKREVDLPI PAQPGPPFPL    50
VTDYCDIVCG FGRGSAELGI PTANVPINQL PKGINDLDLG VYFGFAHIKT 100
VDGQELSVET RRDGRTVVYN YGQYLSEAND DLSVLPMVLS VGKNPFYGND 150
FKTMELHIIH DFKNDFYGAR VKFNILGHIR PELNYTTKEA LIEDINIDIR 200
TAQTVLATPP YQVFKQQL 218
Length:218
Mass (Da):24,537
Last modified:November 1, 1996 - v1
Checksum:iD96995F4F2EBC9F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49701 Genomic DNA. Translation: CAA89722.1.
AY557733 Genomic DNA. Translation: AAS56059.1.
BK006938 Genomic DNA. Translation: DAA12077.1.
PIRiS54532.
RefSeqiNP_010522.1. NM_001180544.1.

Genome annotation databases

EnsemblFungiiYDR236C; YDR236C; YDR236C.
GeneIDi851822.
KEGGisce:YDR236C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49701 Genomic DNA. Translation: CAA89722.1 .
AY557733 Genomic DNA. Translation: AAS56059.1 .
BK006938 Genomic DNA. Translation: DAA12077.1 .
PIRi S54532.
RefSeqi NP_010522.1. NM_001180544.1.

3D structure databases

ProteinModelPortali Q03778.
SMRi Q03778. Positions 57-216.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32287. 11 interactions.
DIPi DIP-1316N.
IntActi Q03778. 1 interaction.
MINTi MINT-406034.
STRINGi 4932.YDR236C.

Proteomic databases

MaxQBi Q03778.
PaxDbi Q03778.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR236C ; YDR236C ; YDR236C .
GeneIDi 851822.
KEGGi sce:YDR236C.

Organism-specific databases

CYGDi YDR236c.
SGDi S000002644. FMN1.

Phylogenomic databases

eggNOGi COG0196.
GeneTreei ENSGT00390000015537.
HOGENOMi HOG000260803.
KOi K00861.
OMAi CDIVCGF.
OrthoDBi EOG7K3V05.

Enzyme and pathway databases

UniPathwayi UPA00276 ; UER00406 .
BioCyci MetaCyc:YDR236C-MONOMER.
YEAST:YDR236C-MONOMER.

Miscellaneous databases

NextBioi 969695.
PROi Q03778.

Gene expression databases

Genevestigatori Q03778.

Family and domain databases

Gene3Di 2.40.30.30. 1 hit.
InterProi IPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view ]
PANTHERi PTHR22749. PTHR22749. 1 hit.
Pfami PF01687. Flavokinase. 1 hit.
[Graphical view ]
SMARTi SM00904. Flavokinase. 1 hit.
[Graphical view ]
SUPFAMi SSF82114. SSF82114. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Molecular characterization of FMN1, the structural gene for the monofunctional flavokinase of Saccharomyces cerevisiae."
    Santos M.A., Jimenez A., Revuelta J.L.
    J. Biol. Chem. 275:28618-28624(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRIFK_YEAST
AccessioniPrimary (citable) accession number: Q03778
Secondary accession number(s): D6VSL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

FMN1 is essential for growth on rich medium.
Present with 830 molecules/cell in log phase SD medium.

Caution

The subcellular location of this enzyme needs further confirmation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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