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Protein

Glucan endo-1,3-beta-glucosidase

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Is thought to be an important plant defense-related product against fungal pathogens. Is capable of releasing soluble and highly active elicitor molecules from fungus cell walls.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei270 – 2701NucleophileBy similarity
Active sitei327 – 3271Proton donorBy similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827 Componenti: Chromosome 3

Subcellular locationi

  1. Vacuole By similarity

  2. Note: In intact tissues.By similarity

GO - Cellular componenti

  1. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 347315Glucan endo-1,3-beta-glucosidasePRO_0000011866Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PRIDEiQ03773.
ProMEXiQ03773.

Expressioni

Inductioni

By ethylene.

Gene expression databases

GenevestigatoriQ03773.

Structurei

3D structure databases

ProteinModelPortaliQ03773.
SMRiQ03773. Positions 35-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ03773.
OMAiARTYVNN.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKYHSSGKS SSMTAIAFLF ILLITYTGTT DAQSGVCYGR LGNNLPTPQE
60 70 80 90 100
VVALYNQANI RRMRIYGPSP EVLEALRGSN IELLLDIPND NLRNLASSQD
110 120 130 140 150
NANKWVQDNI KNYANNVRFR YVSVGNEVKP EHSFAQFLVP ALENIQRAIS
160 170 180 190 200
NAGLGNQVKV STAIDTGALA ESFPPSKGSF KSDYRGAYLD GVIRFLVNNN
210 220 230 240 250
APLMVNVYSY FAYTANPKDI SLDYALFRSP SVVVQDGSLG YRNLFDASVD
260 270 280 290 300
AVYAALEKAG GGSLNIVVSE SGWPSSGGTA TSLDNARTYN TNLVRNVKQG
310 320 330 340
TPKRPGAPLE TYVFAMFDEN QKQPEFEKFW GLFSPITKQP KYSINFN
Length:347
Mass (Da):38,112
Last modified:October 1, 1996 - v1
Checksum:i0FB1B814FDF78753
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37753 mRNA. Translation: AAA33946.1.
PIRiT07108.
RefSeqiNP_001238474.1. NM_001251545.1.
UniGeneiGma.31973.

Genome annotation databases

EnsemblPlantsiGLYMA03G28850.1; GLYMA03G28850.1; GLYMA03G28850.
GeneIDi547822.
KEGGigmx:547822.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37753 mRNA. Translation: AAA33946.1.
PIRiT07108.
RefSeqiNP_001238474.1. NM_001251545.1.
UniGeneiGma.31973.

3D structure databases

ProteinModelPortaliQ03773.
SMRiQ03773. Positions 35-346.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Proteomic databases

PRIDEiQ03773.
ProMEXiQ03773.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGLYMA03G28850.1; GLYMA03G28850.1; GLYMA03G28850.
GeneIDi547822.
KEGGigmx:547822.

Phylogenomic databases

InParanoidiQ03773.
OMAiARTYVNN.

Gene expression databases

GenevestigatoriQ03773.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and ethylene induction of mRNA encoding a phytoalexin elicitor-releasing factor, beta-1,3-endoglucanase, in soybean."
    Takeuchi Y., Yoshikawa M., Takeba G., Tanaka K., Shibata D., Horino O.
    Plant Physiol. 93:673-682(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 186-194 AND 219-224.
    Strain: cv. Harosoy 63.
    Tissue: Cotyledon.

Entry informationi

Entry nameiE13A_SOYBN
AccessioniPrimary (citable) accession number: Q03773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.