Q03773 (E13A_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glucan endo-1,3-beta-glucosidase EC=3.2.1.39 Alternative name(s): (1->3)-beta-glucan endohydrolase Short name=(1->3)-beta-glucanase Beta-1,3-endoglucanase
Organism	Glycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier	3847 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is thought to be an important plant defense-related product against fungal pathogens. Is capable of releasing soluble and highly active elicitor molecules from fungus cell walls.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Vacuole By similarity. Note: In intact tissues By similarity.
Induction	By ethylene.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 347	315	Glucan endo-1,3-beta-glucosidase		PRO_0000011866
Sites
	Active site	270	1	Nucleophile By similarity
	Active site	327	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03773 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0FB1B814FDF78753	FASTA	347	38,112
	10 20 30 40 50 60 MAKYHSSGKS SSMTAIAFLF ILLITYTGTT DAQSGVCYGR LGNNLPTPQE VVALYNQANI 70 80 90 100 110 120 RRMRIYGPSP EVLEALRGSN IELLLDIPND NLRNLASSQD NANKWVQDNI KNYANNVRFR 130 140 150 160 170 180 YVSVGNEVKP EHSFAQFLVP ALENIQRAIS NAGLGNQVKV STAIDTGALA ESFPPSKGSF 190 200 210 220 230 240 KSDYRGAYLD GVIRFLVNNN APLMVNVYSY FAYTANPKDI SLDYALFRSP SVVVQDGSLG 250 260 270 280 290 300 YRNLFDASVD AVYAALEKAG GGSLNIVVSE SGWPSSGGTA TSLDNARTYN TNLVRNVKQG 310 320 330 340 TPKRPGAPLE TYVFAMFDEN QKQPEFEKFW GLFSPITKQP KYSINFN

References

[1]

"Molecular cloning and ethylene induction of mRNA encoding a phytoalexin elicitor-releasing factor, beta-1,3-endoglucanase, in soybean." Takeuchi Y., Yoshikawa M., Takeba G., Tanaka K., Shibata D., Horino O. Plant Physiol. 93:673-682(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 186-194 AND 219-224. Strain: cv. Harosoy 63. Tissue: Cotyledon.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37753 mRNA. Translation: AAA33946.1.
PIR	T07108.
RefSeq	NP_001238474.1. NM_001251545.1.
UniGene	Gma.31973.
3D structure databases
ProteinModelPortal	Q03773.
SMR	Q03773. Positions 35-346.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Proteomic databases
ProMEX	Q03773.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	GLYMA03G28850.1; GLYMA03G28850.1; GLYMA03G28850.
GeneID	547822.
KEGG	gmx:547822.
Gene expression databases
Genevestigator	Q03773.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13A_SOYBN

Accession

Primary (citable) accession number: Q03773

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program