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Q03770

- SSY1_YEAST

UniProt

Q03770 - SSY1_YEAST

Protein

SPS-sensor component SSY1

Gene

SSY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Amino acid sensor component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2. Amino-acid permease homolog that seems to interact directly with the extracellular signaling molecules, but has no amino acid transporter activity. May recruit casein kinases YCK1 and YCK2 to hyperphosphorylate and activate downstream component PTR3 in response to amino acid stimulus.9 Publications

    GO - Molecular functioni

    1. amino acid transmembrane transporter activity Source: InterPro

    GO - Biological processi

    1. response to amino acid Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29750-MONOMER.

    Protein family/group databases

    TCDBi2.A.3.10.12. the amino acid-polyamine-organocation (apc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SPS-sensor component SSY1
    Alternative name(s):
    Amino-acid permease homolog SSY1
    Gene namesi
    Name:SSY1
    Synonyms:APF7, SHR10
    Ordered Locus Names:YDR160W
    ORF Names:YD8358.14
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR160w.
    SGDiS000002567. SSY1.

    Subcellular locationi

    Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi382 – 3821T → H or L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids. 1 Publication
    Mutagenesisi382 – 3821T → K in SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids. 1 Publication
    Mutagenesisi382 – 3821T → R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 852852SPS-sensor component SSY1PRO_0000054164Add
    BLAST

    Proteomic databases

    PaxDbiQ03770.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03770.

    Interactioni

    Subunit structurei

    Component of the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor complex. Interacts directly with PTR3 and SSY5. Interacts with YCK1.1 Publication

    Protein-protein interaction databases

    BioGridi32211. 99 interactions.
    DIPiDIP-2920N.
    IntActiQ03770. 4 interactions.
    MINTiMINT-1733718.
    STRINGi4932.YDR160W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03770.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 285285CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini307 – 32923ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini351 – 3577CytoplasmicSequence Analysis
    Topological domaini379 – 40022ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini422 – 4221CytoplasmicSequence Analysis
    Topological domaini444 – 50057ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini522 – 54019CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini562 – 62362ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini645 – 67329CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini695 – 7039ExtracellularSequence Analysis
    Topological domaini725 – 75531CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini777 – 7848ExtracellularSequence Analysis
    Topological domaini806 – 85247CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei286 – 30621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei330 – 35021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei358 – 37821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei401 – 42121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei423 – 44321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei501 – 52121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei541 – 56121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei624 – 64421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei674 – 69421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei704 – 72421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei756 – 77621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei785 – 80521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0833.
    GeneTreeiENSGT00510000049744.
    HOGENOMiHOG000065954.
    KOiK16261.
    OMAiSFCEMVT.
    OrthoDBiEOG70W3NT.

    Family and domain databases

    InterProiIPR004841. AA-permease/SLC12A_dom.
    IPR002293. AA/rel_permease1.
    IPR004762. Amino_acid_permease_fungi.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF00324. AA_permease. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00913. 2A0310. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q03770-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSVNQIYDL FPNKHNIQFT DSHSQEHDTS SSLAKNDTDG TISIPGSIDT    50
    GILKSIIEEQ GWNDAELYRS SIQNQRFFLT DKYTKKKHLT MEDMLSPEEE 100
    QIYQEPIQDF QTYNKRVQRE YELRERMEEF FRQNTKNDLH ILNEDSLNQQ 150
    YSPLGPADYV LPLDRYSRMK HIASNFFRKK LGIPRKLKRR SHYNPNAEGH 200
    TKGNSSILSS TTDVIDNASY RNIAIDENVD ITHKEHAIDE INEQGASGSE 250
    SVVEGGSLLH DIEKVFNRSR ATRKYHIQRK LKVRHIQMLS IGACFSVGLF 300
    LTSGKAFSIA GPFGTLLGFG LTGSIILATM LSFTELSTLI PVSSGFSGLA 350
    SRFVEDAFGF ALGWTYWISC MLALPAQVSS STFYLSYYNN VNISKGVTAG 400
    FITLFSAFSI VVNLLDVSIM GEIVYVAGIS KVIIAILMVF TMIILNAGHG 450
    NDIHEGVGFR YWDSSKSVRN LTYGLYRPTF DLADAGEGSK KGISGPKGRF 500
    LATASVMLIS TFAFSGVEMT FLASGEAINP RKTIPSATKR TFSIVLISYV 550
    FLIFSVGINI YSGDPRLLSY FPGISEKRYE AIIKGTGMDW RLRTNCRGGI 600
    DYRQISVGTG YSSPWVVALQ NFGLCTFASA FNAILIFFTA TAGISSLFSC 650
    SRTLYAMSVQ RKAPPVFEIC SKRGVPYVSV IFSSLFSVIA YIAVDQTAIE 700
    NFDVLANVSS ASTSIIWMGL NLSFLRFYYA LKQRKDIISR NDSSYPYKSP 750
    FQPYLAIYGL VGCSLFVIFM GYPNFIHHFW STKAFFSAYG GLMFFFISYT 800
    AYKVLGTSKI QRLDQLDMDS GRREMDRTDW TEHSQYLGTY RERAKKLVTW 850
    LI 852
    Length:852
    Mass (Da):95,744
    Last modified:November 1, 1996 - v1
    Checksum:i81A94141DF4EEF7D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50046 Genomic DNA. Translation: CAA90380.1.
    BK006938 Genomic DNA. Translation: DAA12000.1.
    PIRiS57984.
    RefSeqiNP_010444.1. NM_001180467.1.

    Genome annotation databases

    EnsemblFungiiYDR160W; YDR160W; YDR160W.
    GeneIDi851738.
    KEGGisce:YDR160W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50046 Genomic DNA. Translation: CAA90380.1 .
    BK006938 Genomic DNA. Translation: DAA12000.1 .
    PIRi S57984.
    RefSeqi NP_010444.1. NM_001180467.1.

    3D structure databases

    ProteinModelPortali Q03770.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32211. 99 interactions.
    DIPi DIP-2920N.
    IntActi Q03770. 4 interactions.
    MINTi MINT-1733718.
    STRINGi 4932.YDR160W.

    Protein family/group databases

    TCDBi 2.A.3.10.12. the amino acid-polyamine-organocation (apc) family.

    Proteomic databases

    PaxDbi Q03770.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR160W ; YDR160W ; YDR160W .
    GeneIDi 851738.
    KEGGi sce:YDR160W.

    Organism-specific databases

    CYGDi YDR160w.
    SGDi S000002567. SSY1.

    Phylogenomic databases

    eggNOGi COG0833.
    GeneTreei ENSGT00510000049744.
    HOGENOMi HOG000065954.
    KOi K16261.
    OMAi SFCEMVT.
    OrthoDBi EOG70W3NT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29750-MONOMER.

    Miscellaneous databases

    NextBioi 969469.

    Gene expression databases

    Genevestigatori Q03770.

    Family and domain databases

    InterProi IPR004841. AA-permease/SLC12A_dom.
    IPR002293. AA/rel_permease1.
    IPR004762. Amino_acid_permease_fungi.
    [Graphical view ]
    PANTHERi PTHR11785. PTHR11785. 1 hit.
    Pfami PF00324. AA_permease. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00913. 2A0310. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The permease homologue Ssy1p controls the expression of amino acid and peptide transporter genes in Saccharomyces cerevisiae."
      Didion T., Regenberg B., Joergensen M.U., Kielland-Brandt M.C., Andersen H.A.
      Mol. Microbiol. 27:643-650(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Mutations in five loci affecting GAP1-independent uptake of neutral amino acids in yeast."
      Joergensen M.U., Bruun M.B., Didion T., Kielland-Brandt M.C.
      Yeast 14:103-114(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease."
      Iraqui I., Vissers S., Bernard F., de Craene J.-O., Boles E., Urrestarazu A., Andre B.
      Mol. Cell. Biol. 19:989-1001(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids."
      Klasson H., Fink G.R., Ljungdahl P.O.
      Mol. Cell. Biol. 19:5405-5416(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids."
      Forsberg H., Ljungdahl P.O.
      Mol. Cell. Biol. 21:814-826(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "The role of the yeast plasma membrane SPS nutrient sensor in the metabolic response to extracellular amino acids."
      Forsberg H., Gilstring C.F., Zargari A., Martinez P., Ljungdahl P.O.
      Mol. Microbiol. 42:215-228(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Genome-wide expression analysis of genes affected by amino acid sensor Ssy1p in Saccharomyces cerevisiae."
      Kodama Y., Omura F., Takahashi K., Shirahige K., Ashikari T.
      Curr. Genet. 41:63-72(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Receptor-mediated endoproteolytic activation of two transcription factors in yeast."
      Andreasson C., Ljungdahl P.O.
      Genes Dev. 16:3158-3172(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Constitutive and hyperresponsive signaling by mutant forms of Saccharomyces cerevisiae amino acid sensor Ssy1."
      Gaber R.F., Ottow K., Andersen H.A., Kielland-Brandt M.C.
      Eukaryot. Cell 2:922-929(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF THR-382.
    12. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    13. "Activation of the SPS amino acid-sensing pathway in Saccharomyces cerevisiae correlates with the phosphorylation state of a sensor component, Ptr3."
      Liu Z., Thornton J., Spirek M., Butow R.A.
      Mol. Cell. Biol. 28:551-563(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PTR3; SSY5 AND YCK1.

    Entry informationi

    Entry nameiSSY1_YEAST
    AccessioniPrimary (citable) accession number: Q03770
    Secondary accession number(s): D6VSE0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3