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Q03770

- SSY1_YEAST

UniProt

Q03770 - SSY1_YEAST

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Protein
SPS-sensor component SSY1
Gene
SSY1, APF7, SHR10, YDR160W, YD8358.14
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Amino acid sensor component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2. Amino-acid permease homolog that seems to interact directly with the extracellular signaling molecules, but has no amino acid transporter activity. May recruit casein kinases YCK1 and YCK2 to hyperphosphorylate and activate downstream component PTR3 in response to amino acid stimulus.9 Publications

GO - Molecular functioni

  1. amino acid transmembrane transporter activity Source: InterPro

GO - Biological processi

  1. response to amino acid Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29750-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.12. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
SPS-sensor component SSY1
Alternative name(s):
Amino-acid permease homolog SSY1
Gene namesi
Name:SSY1
Synonyms:APF7, SHR10
Ordered Locus Names:YDR160W
ORF Names:YD8358.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR160w.
SGDiS000002567. SSY1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 285285Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei286 – 30621Helical; Reviewed prediction
Add
BLAST
Topological domaini307 – 32923Extracellular Reviewed prediction
Add
BLAST
Transmembranei330 – 35021Helical; Reviewed prediction
Add
BLAST
Topological domaini351 – 3577Cytoplasmic Reviewed prediction
Transmembranei358 – 37821Helical; Reviewed prediction
Add
BLAST
Topological domaini379 – 40022Extracellular Reviewed prediction
Add
BLAST
Transmembranei401 – 42121Helical; Reviewed prediction
Add
BLAST
Topological domaini422 – 4221Cytoplasmic Reviewed prediction
Transmembranei423 – 44321Helical; Reviewed prediction
Add
BLAST
Topological domaini444 – 50057Extracellular Reviewed prediction
Add
BLAST
Transmembranei501 – 52121Helical; Reviewed prediction
Add
BLAST
Topological domaini522 – 54019Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei541 – 56121Helical; Reviewed prediction
Add
BLAST
Topological domaini562 – 62362Extracellular Reviewed prediction
Add
BLAST
Transmembranei624 – 64421Helical; Reviewed prediction
Add
BLAST
Topological domaini645 – 67329Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei674 – 69421Helical; Reviewed prediction
Add
BLAST
Topological domaini695 – 7039Extracellular Reviewed prediction
Transmembranei704 – 72421Helical; Reviewed prediction
Add
BLAST
Topological domaini725 – 75531Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei756 – 77621Helical; Reviewed prediction
Add
BLAST
Topological domaini777 – 7848Extracellular Reviewed prediction
Transmembranei785 – 80521Helical; Reviewed prediction
Add
BLAST
Topological domaini806 – 85247Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi382 – 3821T → H or L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids. 1 Publication
Mutagenesisi382 – 3821T → K in SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids. 1 Publication
Mutagenesisi382 – 3821T → R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 852852SPS-sensor component SSY1
PRO_0000054164Add
BLAST

Proteomic databases

PaxDbiQ03770.

Expressioni

Gene expression databases

GenevestigatoriQ03770.

Interactioni

Subunit structurei

Component of the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor complex. Interacts directly with PTR3 and SSY5. Interacts with YCK1.1 Publication

Protein-protein interaction databases

BioGridi32211. 99 interactions.
DIPiDIP-2920N.
IntActiQ03770. 4 interactions.
MINTiMINT-1733718.
STRINGi4932.YDR160W.

Structurei

3D structure databases

ProteinModelPortaliQ03770.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0833.
GeneTreeiENSGT00510000049744.
HOGENOMiHOG000065954.
KOiK16261.
OMAiSFCEMVT.
OrthoDBiEOG70W3NT.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03770-1 [UniParc]FASTAAdd to Basket

« Hide

MSSVNQIYDL FPNKHNIQFT DSHSQEHDTS SSLAKNDTDG TISIPGSIDT    50
GILKSIIEEQ GWNDAELYRS SIQNQRFFLT DKYTKKKHLT MEDMLSPEEE 100
QIYQEPIQDF QTYNKRVQRE YELRERMEEF FRQNTKNDLH ILNEDSLNQQ 150
YSPLGPADYV LPLDRYSRMK HIASNFFRKK LGIPRKLKRR SHYNPNAEGH 200
TKGNSSILSS TTDVIDNASY RNIAIDENVD ITHKEHAIDE INEQGASGSE 250
SVVEGGSLLH DIEKVFNRSR ATRKYHIQRK LKVRHIQMLS IGACFSVGLF 300
LTSGKAFSIA GPFGTLLGFG LTGSIILATM LSFTELSTLI PVSSGFSGLA 350
SRFVEDAFGF ALGWTYWISC MLALPAQVSS STFYLSYYNN VNISKGVTAG 400
FITLFSAFSI VVNLLDVSIM GEIVYVAGIS KVIIAILMVF TMIILNAGHG 450
NDIHEGVGFR YWDSSKSVRN LTYGLYRPTF DLADAGEGSK KGISGPKGRF 500
LATASVMLIS TFAFSGVEMT FLASGEAINP RKTIPSATKR TFSIVLISYV 550
FLIFSVGINI YSGDPRLLSY FPGISEKRYE AIIKGTGMDW RLRTNCRGGI 600
DYRQISVGTG YSSPWVVALQ NFGLCTFASA FNAILIFFTA TAGISSLFSC 650
SRTLYAMSVQ RKAPPVFEIC SKRGVPYVSV IFSSLFSVIA YIAVDQTAIE 700
NFDVLANVSS ASTSIIWMGL NLSFLRFYYA LKQRKDIISR NDSSYPYKSP 750
FQPYLAIYGL VGCSLFVIFM GYPNFIHHFW STKAFFSAYG GLMFFFISYT 800
AYKVLGTSKI QRLDQLDMDS GRREMDRTDW TEHSQYLGTY RERAKKLVTW 850
LI 852
Length:852
Mass (Da):95,744
Last modified:November 1, 1996 - v1
Checksum:i81A94141DF4EEF7D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50046 Genomic DNA. Translation: CAA90380.1.
BK006938 Genomic DNA. Translation: DAA12000.1.
PIRiS57984.
RefSeqiNP_010444.1. NM_001180467.1.

Genome annotation databases

EnsemblFungiiYDR160W; YDR160W; YDR160W.
GeneIDi851738.
KEGGisce:YDR160W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50046 Genomic DNA. Translation: CAA90380.1 .
BK006938 Genomic DNA. Translation: DAA12000.1 .
PIRi S57984.
RefSeqi NP_010444.1. NM_001180467.1.

3D structure databases

ProteinModelPortali Q03770.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32211. 99 interactions.
DIPi DIP-2920N.
IntActi Q03770. 4 interactions.
MINTi MINT-1733718.
STRINGi 4932.YDR160W.

Protein family/group databases

TCDBi 2.A.3.10.12. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbi Q03770.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR160W ; YDR160W ; YDR160W .
GeneIDi 851738.
KEGGi sce:YDR160W.

Organism-specific databases

CYGDi YDR160w.
SGDi S000002567. SSY1.

Phylogenomic databases

eggNOGi COG0833.
GeneTreei ENSGT00510000049744.
HOGENOMi HOG000065954.
KOi K16261.
OMAi SFCEMVT.
OrthoDBi EOG70W3NT.

Enzyme and pathway databases

BioCyci YEAST:G3O-29750-MONOMER.

Miscellaneous databases

NextBioi 969469.

Gene expression databases

Genevestigatori Q03770.

Family and domain databases

InterProi IPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
[Graphical view ]
PANTHERi PTHR11785. PTHR11785. 1 hit.
Pfami PF00324. AA_permease. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00913. 2A0310. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The permease homologue Ssy1p controls the expression of amino acid and peptide transporter genes in Saccharomyces cerevisiae."
    Didion T., Regenberg B., Joergensen M.U., Kielland-Brandt M.C., Andersen H.A.
    Mol. Microbiol. 27:643-650(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Mutations in five loci affecting GAP1-independent uptake of neutral amino acids in yeast."
    Joergensen M.U., Bruun M.B., Didion T., Kielland-Brandt M.C.
    Yeast 14:103-114(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease."
    Iraqui I., Vissers S., Bernard F., de Craene J.-O., Boles E., Urrestarazu A., Andre B.
    Mol. Cell. Biol. 19:989-1001(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids."
    Klasson H., Fink G.R., Ljungdahl P.O.
    Mol. Cell. Biol. 19:5405-5416(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids."
    Forsberg H., Ljungdahl P.O.
    Mol. Cell. Biol. 21:814-826(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "The role of the yeast plasma membrane SPS nutrient sensor in the metabolic response to extracellular amino acids."
    Forsberg H., Gilstring C.F., Zargari A., Martinez P., Ljungdahl P.O.
    Mol. Microbiol. 42:215-228(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Genome-wide expression analysis of genes affected by amino acid sensor Ssy1p in Saccharomyces cerevisiae."
    Kodama Y., Omura F., Takahashi K., Shirahige K., Ashikari T.
    Curr. Genet. 41:63-72(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Receptor-mediated endoproteolytic activation of two transcription factors in yeast."
    Andreasson C., Ljungdahl P.O.
    Genes Dev. 16:3158-3172(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Constitutive and hyperresponsive signaling by mutant forms of Saccharomyces cerevisiae amino acid sensor Ssy1."
    Gaber R.F., Ottow K., Andersen H.A., Kielland-Brandt M.C.
    Eukaryot. Cell 2:922-929(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF THR-382.
  12. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  13. "Activation of the SPS amino acid-sensing pathway in Saccharomyces cerevisiae correlates with the phosphorylation state of a sensor component, Ptr3."
    Liu Z., Thornton J., Spirek M., Butow R.A.
    Mol. Cell. Biol. 28:551-563(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PTR3; SSY5 AND YCK1.

Entry informationi

Entry nameiSSY1_YEAST
AccessioniPrimary (citable) accession number: Q03770
Secondary accession number(s): D6VSE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi