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Protein

SPS-sensor component SSY1

Gene

SSY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Amino acid sensor component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2. Amino-acid permease homolog that seems to interact directly with the extracellular signaling molecules, but has no amino acid transporter activity. May recruit casein kinases YCK1 and YCK2 to hyperphosphorylate and activate downstream component PTR3 in response to amino acid stimulus.9 Publications

GO - Molecular functioni

GO - Biological processi

  • response to amino acid Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29750-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.12. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
SPS-sensor component SSY1
Alternative name(s):
Amino-acid permease homolog SSY1
Gene namesi
Name:SSY1
Synonyms:APF7, SHR10
Ordered Locus Names:YDR160W
ORF Names:YD8358.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR160W.
SGDiS000002567. SSY1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 285CytoplasmicSequence analysisAdd BLAST285
Transmembranei286 – 306HelicalSequence analysisAdd BLAST21
Topological domaini307 – 329ExtracellularSequence analysisAdd BLAST23
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
Topological domaini351 – 357CytoplasmicSequence analysis7
Transmembranei358 – 378HelicalSequence analysisAdd BLAST21
Topological domaini379 – 400ExtracellularSequence analysisAdd BLAST22
Transmembranei401 – 421HelicalSequence analysisAdd BLAST21
Topological domaini422CytoplasmicSequence analysis1
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 500ExtracellularSequence analysisAdd BLAST57
Transmembranei501 – 521HelicalSequence analysisAdd BLAST21
Topological domaini522 – 540CytoplasmicSequence analysisAdd BLAST19
Transmembranei541 – 561HelicalSequence analysisAdd BLAST21
Topological domaini562 – 623ExtracellularSequence analysisAdd BLAST62
Transmembranei624 – 644HelicalSequence analysisAdd BLAST21
Topological domaini645 – 673CytoplasmicSequence analysisAdd BLAST29
Transmembranei674 – 694HelicalSequence analysisAdd BLAST21
Topological domaini695 – 703ExtracellularSequence analysis9
Transmembranei704 – 724HelicalSequence analysisAdd BLAST21
Topological domaini725 – 755CytoplasmicSequence analysisAdd BLAST31
Transmembranei756 – 776HelicalSequence analysisAdd BLAST21
Topological domaini777 – 784ExtracellularSequence analysis8
Transmembranei785 – 805HelicalSequence analysisAdd BLAST21
Topological domaini806 – 852CytoplasmicSequence analysisAdd BLAST47

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
  • vacuole Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi382T → H or L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids. 1 Publication1
Mutagenesisi382T → K in SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids. 1 Publication1
Mutagenesisi382T → R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541641 – 852SPS-sensor component SSY1Add BLAST852

Proteomic databases

PRIDEiQ03770.

Interactioni

Subunit structurei

Component of the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor complex. Interacts directly with PTR3 and SSY5. Interacts with YCK1.1 Publication

Protein-protein interaction databases

BioGridi32211. 98 interactors.
DIPiDIP-2920N.
IntActiQ03770. 4 interactors.
MINTiMINT-1733718.

Structurei

3D structure databases

ProteinModelPortaliQ03770.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000065954.
InParanoidiQ03770.
KOiK16261.
OMAiSFCEMVT.
OrthoDBiEOG092C164R.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 3 hits.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03770-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVNQIYDL FPNKHNIQFT DSHSQEHDTS SSLAKNDTDG TISIPGSIDT
60 70 80 90 100
GILKSIIEEQ GWNDAELYRS SIQNQRFFLT DKYTKKKHLT MEDMLSPEEE
110 120 130 140 150
QIYQEPIQDF QTYNKRVQRE YELRERMEEF FRQNTKNDLH ILNEDSLNQQ
160 170 180 190 200
YSPLGPADYV LPLDRYSRMK HIASNFFRKK LGIPRKLKRR SHYNPNAEGH
210 220 230 240 250
TKGNSSILSS TTDVIDNASY RNIAIDENVD ITHKEHAIDE INEQGASGSE
260 270 280 290 300
SVVEGGSLLH DIEKVFNRSR ATRKYHIQRK LKVRHIQMLS IGACFSVGLF
310 320 330 340 350
LTSGKAFSIA GPFGTLLGFG LTGSIILATM LSFTELSTLI PVSSGFSGLA
360 370 380 390 400
SRFVEDAFGF ALGWTYWISC MLALPAQVSS STFYLSYYNN VNISKGVTAG
410 420 430 440 450
FITLFSAFSI VVNLLDVSIM GEIVYVAGIS KVIIAILMVF TMIILNAGHG
460 470 480 490 500
NDIHEGVGFR YWDSSKSVRN LTYGLYRPTF DLADAGEGSK KGISGPKGRF
510 520 530 540 550
LATASVMLIS TFAFSGVEMT FLASGEAINP RKTIPSATKR TFSIVLISYV
560 570 580 590 600
FLIFSVGINI YSGDPRLLSY FPGISEKRYE AIIKGTGMDW RLRTNCRGGI
610 620 630 640 650
DYRQISVGTG YSSPWVVALQ NFGLCTFASA FNAILIFFTA TAGISSLFSC
660 670 680 690 700
SRTLYAMSVQ RKAPPVFEIC SKRGVPYVSV IFSSLFSVIA YIAVDQTAIE
710 720 730 740 750
NFDVLANVSS ASTSIIWMGL NLSFLRFYYA LKQRKDIISR NDSSYPYKSP
760 770 780 790 800
FQPYLAIYGL VGCSLFVIFM GYPNFIHHFW STKAFFSAYG GLMFFFISYT
810 820 830 840 850
AYKVLGTSKI QRLDQLDMDS GRREMDRTDW TEHSQYLGTY RERAKKLVTW

LI
Length:852
Mass (Da):95,744
Last modified:November 1, 1996 - v1
Checksum:i81A94141DF4EEF7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50046 Genomic DNA. Translation: CAA90380.1.
BK006938 Genomic DNA. Translation: DAA12000.1.
PIRiS57984.
RefSeqiNP_010444.1. NM_001180467.1.

Genome annotation databases

EnsemblFungiiYDR160W; YDR160W; YDR160W.
GeneIDi851738.
KEGGisce:YDR160W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50046 Genomic DNA. Translation: CAA90380.1.
BK006938 Genomic DNA. Translation: DAA12000.1.
PIRiS57984.
RefSeqiNP_010444.1. NM_001180467.1.

3D structure databases

ProteinModelPortaliQ03770.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32211. 98 interactors.
DIPiDIP-2920N.
IntActiQ03770. 4 interactors.
MINTiMINT-1733718.

Protein family/group databases

TCDBi2.A.3.10.12. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PRIDEiQ03770.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR160W; YDR160W; YDR160W.
GeneIDi851738.
KEGGisce:YDR160W.

Organism-specific databases

EuPathDBiFungiDB:YDR160W.
SGDiS000002567. SSY1.

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000065954.
InParanoidiQ03770.
KOiK16261.
OMAiSFCEMVT.
OrthoDBiEOG092C164R.

Enzyme and pathway databases

BioCyciYEAST:G3O-29750-MONOMER.

Miscellaneous databases

PROiQ03770.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 3 hits.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSSY1_YEAST
AccessioniPrimary (citable) accession number: Q03770
Secondary accession number(s): D6VSE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.