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Q03770

- SSY1_YEAST

UniProt

Q03770 - SSY1_YEAST

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Protein

SPS-sensor component SSY1

Gene

SSY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Amino acid sensor component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2. Amino-acid permease homolog that seems to interact directly with the extracellular signaling molecules, but has no amino acid transporter activity. May recruit casein kinases YCK1 and YCK2 to hyperphosphorylate and activate downstream component PTR3 in response to amino acid stimulus.9 Publications

GO - Molecular functioni

  1. amino acid transmembrane transporter activity Source: InterPro

GO - Biological processi

  1. response to amino acid Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29750-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.12. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
SPS-sensor component SSY1
Alternative name(s):
Amino-acid permease homolog SSY1
Gene namesi
Name:SSY1
Synonyms:APF7, SHR10
Ordered Locus Names:YDR160W
ORF Names:YD8358.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR160w.
SGDiS000002567. SSY1.

Subcellular locationi

Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi382 – 3821T → H or L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids. 1 Publication
Mutagenesisi382 – 3821T → K in SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids. 1 Publication
Mutagenesisi382 – 3821T → R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 852852SPS-sensor component SSY1PRO_0000054164Add
BLAST

Proteomic databases

PaxDbiQ03770.

Expressioni

Gene expression databases

GenevestigatoriQ03770.

Interactioni

Subunit structurei

Component of the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor complex. Interacts directly with PTR3 and SSY5. Interacts with YCK1.1 Publication

Protein-protein interaction databases

BioGridi32211. 99 interactions.
DIPiDIP-2920N.
IntActiQ03770. 4 interactions.
MINTiMINT-1733718.
STRINGi4932.YDR160W.

Structurei

3D structure databases

ProteinModelPortaliQ03770.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 285285CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini307 – 32923ExtracellularSequence AnalysisAdd
BLAST
Topological domaini351 – 3577CytoplasmicSequence Analysis
Topological domaini379 – 40022ExtracellularSequence AnalysisAdd
BLAST
Topological domaini422 – 4221CytoplasmicSequence Analysis
Topological domaini444 – 50057ExtracellularSequence AnalysisAdd
BLAST
Topological domaini522 – 54019CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini562 – 62362ExtracellularSequence AnalysisAdd
BLAST
Topological domaini645 – 67329CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini695 – 7039ExtracellularSequence Analysis
Topological domaini725 – 75531CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini777 – 7848ExtracellularSequence Analysis
Topological domaini806 – 85247CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei286 – 30621HelicalSequence AnalysisAdd
BLAST
Transmembranei330 – 35021HelicalSequence AnalysisAdd
BLAST
Transmembranei358 – 37821HelicalSequence AnalysisAdd
BLAST
Transmembranei401 – 42121HelicalSequence AnalysisAdd
BLAST
Transmembranei423 – 44321HelicalSequence AnalysisAdd
BLAST
Transmembranei501 – 52121HelicalSequence AnalysisAdd
BLAST
Transmembranei541 – 56121HelicalSequence AnalysisAdd
BLAST
Transmembranei624 – 64421HelicalSequence AnalysisAdd
BLAST
Transmembranei674 – 69421HelicalSequence AnalysisAdd
BLAST
Transmembranei704 – 72421HelicalSequence AnalysisAdd
BLAST
Transmembranei756 – 77621HelicalSequence AnalysisAdd
BLAST
Transmembranei785 – 80521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0833.
GeneTreeiENSGT00510000049744.
HOGENOMiHOG000065954.
InParanoidiQ03770.
KOiK16261.
OMAiSFCEMVT.
OrthoDBiEOG70W3NT.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03770-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSVNQIYDL FPNKHNIQFT DSHSQEHDTS SSLAKNDTDG TISIPGSIDT
60 70 80 90 100
GILKSIIEEQ GWNDAELYRS SIQNQRFFLT DKYTKKKHLT MEDMLSPEEE
110 120 130 140 150
QIYQEPIQDF QTYNKRVQRE YELRERMEEF FRQNTKNDLH ILNEDSLNQQ
160 170 180 190 200
YSPLGPADYV LPLDRYSRMK HIASNFFRKK LGIPRKLKRR SHYNPNAEGH
210 220 230 240 250
TKGNSSILSS TTDVIDNASY RNIAIDENVD ITHKEHAIDE INEQGASGSE
260 270 280 290 300
SVVEGGSLLH DIEKVFNRSR ATRKYHIQRK LKVRHIQMLS IGACFSVGLF
310 320 330 340 350
LTSGKAFSIA GPFGTLLGFG LTGSIILATM LSFTELSTLI PVSSGFSGLA
360 370 380 390 400
SRFVEDAFGF ALGWTYWISC MLALPAQVSS STFYLSYYNN VNISKGVTAG
410 420 430 440 450
FITLFSAFSI VVNLLDVSIM GEIVYVAGIS KVIIAILMVF TMIILNAGHG
460 470 480 490 500
NDIHEGVGFR YWDSSKSVRN LTYGLYRPTF DLADAGEGSK KGISGPKGRF
510 520 530 540 550
LATASVMLIS TFAFSGVEMT FLASGEAINP RKTIPSATKR TFSIVLISYV
560 570 580 590 600
FLIFSVGINI YSGDPRLLSY FPGISEKRYE AIIKGTGMDW RLRTNCRGGI
610 620 630 640 650
DYRQISVGTG YSSPWVVALQ NFGLCTFASA FNAILIFFTA TAGISSLFSC
660 670 680 690 700
SRTLYAMSVQ RKAPPVFEIC SKRGVPYVSV IFSSLFSVIA YIAVDQTAIE
710 720 730 740 750
NFDVLANVSS ASTSIIWMGL NLSFLRFYYA LKQRKDIISR NDSSYPYKSP
760 770 780 790 800
FQPYLAIYGL VGCSLFVIFM GYPNFIHHFW STKAFFSAYG GLMFFFISYT
810 820 830 840 850
AYKVLGTSKI QRLDQLDMDS GRREMDRTDW TEHSQYLGTY RERAKKLVTW

LI
Length:852
Mass (Da):95,744
Last modified:November 1, 1996 - v1
Checksum:i81A94141DF4EEF7D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50046 Genomic DNA. Translation: CAA90380.1.
BK006938 Genomic DNA. Translation: DAA12000.1.
PIRiS57984.
RefSeqiNP_010444.1. NM_001180467.1.

Genome annotation databases

EnsemblFungiiYDR160W; YDR160W; YDR160W.
GeneIDi851738.
KEGGisce:YDR160W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50046 Genomic DNA. Translation: CAA90380.1 .
BK006938 Genomic DNA. Translation: DAA12000.1 .
PIRi S57984.
RefSeqi NP_010444.1. NM_001180467.1.

3D structure databases

ProteinModelPortali Q03770.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32211. 99 interactions.
DIPi DIP-2920N.
IntActi Q03770. 4 interactions.
MINTi MINT-1733718.
STRINGi 4932.YDR160W.

Protein family/group databases

TCDBi 2.A.3.10.12. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbi Q03770.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR160W ; YDR160W ; YDR160W .
GeneIDi 851738.
KEGGi sce:YDR160W.

Organism-specific databases

CYGDi YDR160w.
SGDi S000002567. SSY1.

Phylogenomic databases

eggNOGi COG0833.
GeneTreei ENSGT00510000049744.
HOGENOMi HOG000065954.
InParanoidi Q03770.
KOi K16261.
OMAi SFCEMVT.
OrthoDBi EOG70W3NT.

Enzyme and pathway databases

BioCyci YEAST:G3O-29750-MONOMER.

Miscellaneous databases

NextBioi 969469.

Gene expression databases

Genevestigatori Q03770.

Family and domain databases

InterProi IPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
[Graphical view ]
PANTHERi PTHR11785. PTHR11785. 1 hit.
Pfami PF00324. AA_permease. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00913. 2A0310. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The permease homologue Ssy1p controls the expression of amino acid and peptide transporter genes in Saccharomyces cerevisiae."
    Didion T., Regenberg B., Joergensen M.U., Kielland-Brandt M.C., Andersen H.A.
    Mol. Microbiol. 27:643-650(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Mutations in five loci affecting GAP1-independent uptake of neutral amino acids in yeast."
    Joergensen M.U., Bruun M.B., Didion T., Kielland-Brandt M.C.
    Yeast 14:103-114(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease."
    Iraqui I., Vissers S., Bernard F., de Craene J.-O., Boles E., Urrestarazu A., Andre B.
    Mol. Cell. Biol. 19:989-1001(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids."
    Klasson H., Fink G.R., Ljungdahl P.O.
    Mol. Cell. Biol. 19:5405-5416(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids."
    Forsberg H., Ljungdahl P.O.
    Mol. Cell. Biol. 21:814-826(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "The role of the yeast plasma membrane SPS nutrient sensor in the metabolic response to extracellular amino acids."
    Forsberg H., Gilstring C.F., Zargari A., Martinez P., Ljungdahl P.O.
    Mol. Microbiol. 42:215-228(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Genome-wide expression analysis of genes affected by amino acid sensor Ssy1p in Saccharomyces cerevisiae."
    Kodama Y., Omura F., Takahashi K., Shirahige K., Ashikari T.
    Curr. Genet. 41:63-72(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Receptor-mediated endoproteolytic activation of two transcription factors in yeast."
    Andreasson C., Ljungdahl P.O.
    Genes Dev. 16:3158-3172(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Constitutive and hyperresponsive signaling by mutant forms of Saccharomyces cerevisiae amino acid sensor Ssy1."
    Gaber R.F., Ottow K., Andersen H.A., Kielland-Brandt M.C.
    Eukaryot. Cell 2:922-929(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF THR-382.
  12. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  13. "Activation of the SPS amino acid-sensing pathway in Saccharomyces cerevisiae correlates with the phosphorylation state of a sensor component, Ptr3."
    Liu Z., Thornton J., Spirek M., Butow R.A.
    Mol. Cell. Biol. 28:551-563(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PTR3; SSY5 AND YCK1.

Entry informationi

Entry nameiSSY1_YEAST
AccessioniPrimary (citable) accession number: Q03770
Secondary accession number(s): D6VSE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3