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Protein

Epsin-5

Gene

ENT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the recruitment of clathrin to the Golgi network and endosomes to form clathrin coated vesicles. Plays a role in the trafficking of clathrin between the Golgi network and endosomes. Binds to membranes enriched in phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2) and, in association with VPS27, is involved in protein sorting at the multivesicular body (MVB).2 Publications

Miscellaneous

Present with 8100 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • clathrin binding Source: SGD
  • phosphatidylinositol-3,5-bisphosphate binding Source: SGD

GO - Biological processi

  • early endosome to Golgi transport Source: SGD
  • Golgi to endosome transport Source: SGD
  • late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transport
LigandLipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29747-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-5
Gene namesi
Name:ENT5
Ordered Locus Names:YDR153C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR153C.
SGDiS000002560. ENT5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi331F → A: Reduced binding to GGA2. 1 Publication1
Mutagenesisi334F → A: Reduced binding to GGA2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745281 – 411Epsin-5Add BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei322PhosphoserineCombined sources1
Modified residuei324PhosphoserineCombined sources1
Modified residuei363PhosphoserineCombined sources1
Modified residuei368PhosphothreonineCombined sources1
Modified residuei394PhosphoserineCombined sources1
Modified residuei401PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ03769.
PRIDEiQ03769.

PTM databases

iPTMnetiQ03769.

Interactioni

Subunit structurei

Interacts with the clathrin adapter GGA2 and the clathrin adapter complex AP-1.2 Publications

GO - Molecular functioni

  • clathrin binding Source: SGD

Protein-protein interaction databases

BioGridi32206. 173 interactors.
ELMiQ03769.
IntActiQ03769. 1 interactor.
MINTiMINT-2780845.
STRINGi4932.YDR153C.

Structurei

Secondary structure

1411
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 40Combined sources9
Beta strandi43 – 46Combined sources4
Helixi50 – 58Combined sources9
Helixi59 – 61Combined sources3
Helixi65 – 81Combined sources17
Helixi87 – 90Combined sources4
Turni96 – 100Combined sources5
Helixi101 – 118Combined sources18
Helixi123 – 134Combined sources12
Helixi137 – 140Combined sources4
Turni141 – 145Combined sources5
Helixi156 – 175Combined sources20
Helixi177 – 187Combined sources11
Turni188 – 190Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CMWX-ray2.20A31-191[»]
5CMYX-ray2.09A/B31-191[»]
5J08X-ray1.80A31-192[»]
ProteinModelPortaliQ03769.
SMRiQ03769.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 184ENTHPROSITE-ProRule annotationAdd BLAST160

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi304 – 323Asn-richAdd BLAST20

Phylogenomic databases

HOGENOMiHOG000112379.
InParanoidiQ03769.
OMAiPMIYRRF.
OrthoDBiEOG092C3X6H.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiView protein in InterPro
IPR013809. ENTH.
IPR008942. ENTH_VHS.
PfamiView protein in Pfam
PF01417. ENTH. 1 hit.
SMARTiView protein in SMART
SM00273. ENTH. 1 hit.
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiView protein in PROSITE
PS50942. ENTH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03769-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSLSKKIQN LGIHDIRNAA RFAQNVIVQY EPYQIDIRRA TNTDAWGPTP
60 70 80 90 100
KHLAKVLRNR YQVPLYLMTE YTLKRLVDHI ATRPKNLYEK ARKDYVNYGS
110 120 130 140 150
EWRVVLKCLV VIEFLLLNVD TGDELNQIRS CLLTHKHILT REIAQFKVKF
160 170 180 190 200
SNDGKMEIHE RGIRKKGELI LQYLEDSQFL KKERAKNKKN ALKIRQQGES
210 220 230 240 250
SIYNANQIST SASYDNIDDD EFDADADGFD SEMDANNVTN FNVPVETEAN
260 270 280 290 300
SNTRRRSHME EQRRQRREIL REQIKNKEQQ RKRKQQQDSI PDLIDLDDST
310 320 330 340 350
STTNNITIDN GNNDNKNNNI NSNSDDDDDE FGDFQSETSP DTTAPKTSNS
360 370 380 390 400
KIDDLLDWDG PKSDTDTTAA AQTSLPFAEK KQQKARPQAT KDKSKGNDAF
410
SDLFSYSKSL V
Length:411
Mass (Da):47,321
Last modified:November 1, 1996 - v1
Checksum:i8802816008D9A4CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50046 Genomic DNA. Translation: CAA90375.1.
AY557702 Genomic DNA. Translation: AAS56028.1.
BK006938 Genomic DNA. Translation: DAA11995.1.
PIRiS57979.
RefSeqiNP_010437.3. NM_001180460.3.

Genome annotation databases

EnsemblFungiiYDR153C; YDR153C; YDR153C.
GeneIDi851731.
KEGGisce:YDR153C.

Similar proteinsi

Entry informationi

Entry nameiENT5_YEAST
AccessioniPrimary (citable) accession number: Q03769
Secondary accession number(s): D6VSD5, Q6Q5U2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names