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Protein

Ethanolamine kinase

Gene

EKI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the committed step of phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway. Also exhibits choline kinase activity but its preferred substrate is ethanolamine.

Catalytic activityi

ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ethanolamine kinase (EKI1)
  2. Ethanolamine-phosphate cytidylyltransferase (ECT1)
  3. Choline/ethanolaminephosphotransferase 1 (EPT1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • choline kinase activity Source: SGD
  • ethanolamine kinase activity Source: SGD

GO - Biological processi

  • CDP-choline pathway Source: GOC
  • phosphatidylcholine biosynthetic process Source: SGD
  • phosphatidylethanolamine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YDR147W-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.
R-SCE-1483213. Synthesis of PE.
UniPathwayiUPA00558; UER00741.

Chemistry

SwissLipidsiSLP:000000065.

Names & Taxonomyi

Protein namesi
Recommended name:
Ethanolamine kinase (EC:2.7.1.82)
Short name:
EK
Gene namesi
Name:EKI1
Ordered Locus Names:YDR147W
ORF Names:YD8358.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR147W.
SGDiS000002554. EKI1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534Ethanolamine kinasePRO_0000206230Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03764.

PTM databases

iPTMnetiQ03764.

Interactioni

Protein-protein interaction databases

BioGridi32201. 22 interactions.
MINTiMINT-2784774.

Structurei

3D structure databases

ProteinModelPortaliQ03764.
SMRiQ03764. Positions 135-445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000062991.
HOGENOMiHOG000246747.
InParanoidiQ03764.
KOiK00894.
OMAiCHNDLQH.
OrthoDBiEOG7WT48T.

Family and domain databases

InterProiIPR007521. Choline_kin_N.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF04428. Choline_kin_N. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequencei

Sequence statusi: Complete.

Q03764-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYTNYSLTSS DAMPRTYLVG TASPEMSKKK RQSANCDKPT RRVIHIIDTN
60 70 80 90 100
EHSEVDLKNE LPITCTNEDG EMTSSSWTSQ TANDFLKLAY VNAKLDPSLP
110 120 130 140 150
SQYFKQDIIN VLQSLEIPGW SVPGSKESSL NKNLLTLTQI KGALTNVIYK
160 170 180 190 200
IHYPNLPPLL MRIFGDSIDS VIDREYELKV IARLSFYDLG PKLEGFFENG
210 220 230 240 250
RFEKYIEGSR TSTQADFIDR DTSIKIAKKL KELHCTVPLT HKEITDQPSC
260 270 280 290 300
WTTFDQWIKL IDSHKEWVSN NVNISENLRC SSWNFFLKSF KNYKRWLYND
310 320 330 340 350
SAFTSKLLRE DDKDSMINSG LKMVFCHNDL QHGNLLFKSK GKDDISVGDL
360 370 380 390 400
TIIDFEYAGP NPVVFDLSNH LNEWMQDYND VQSFKSHIDK YPKEEDILVF
410 420 430 440 450
AQSYINHMNE NHVKIASQEV RILYNLIIEW RPCTQLFWCL WALLQSGRLP
460 470 480 490 500
QRPLIEGEKL MSEKAGLGDE THLMEHKNKE NGKYDCSEDD SFNYLGFCKE
510 520 530
KMSVFWGDLI TLGVIDKDCP DIGKTDYLDT KLIF
Length:534
Mass (Da):61,657
Last modified:November 1, 1996 - v1
Checksum:i47B417841D5005A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50046 Genomic DNA. Translation: CAA90370.1.
BK006938 Genomic DNA. Translation: DAA11990.1.
PIRiS57974.
RefSeqiNP_010431.3. NM_001180454.3.

Genome annotation databases

EnsemblFungiiYDR147W; YDR147W; YDR147W.
GeneIDi851725.
KEGGisce:YDR147W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50046 Genomic DNA. Translation: CAA90370.1.
BK006938 Genomic DNA. Translation: DAA11990.1.
PIRiS57974.
RefSeqiNP_010431.3. NM_001180454.3.

3D structure databases

ProteinModelPortaliQ03764.
SMRiQ03764. Positions 135-445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32201. 22 interactions.
MINTiMINT-2784774.

Chemistry

SwissLipidsiSLP:000000065.

PTM databases

iPTMnetiQ03764.

Proteomic databases

MaxQBiQ03764.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR147W; YDR147W; YDR147W.
GeneIDi851725.
KEGGisce:YDR147W.

Organism-specific databases

EuPathDBiFungiDB:YDR147W.
SGDiS000002554. EKI1.

Phylogenomic databases

GeneTreeiENSGT00530000062991.
HOGENOMiHOG000246747.
InParanoidiQ03764.
KOiK00894.
OMAiCHNDLQH.
OrthoDBiEOG7WT48T.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00741.
BioCyciYEAST:YDR147W-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.
R-SCE-1483213. Synthesis of PE.

Miscellaneous databases

NextBioi969439.
PROiQ03764.

Family and domain databases

InterProiIPR007521. Choline_kin_N.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF04428. Choline_kin_N. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Isolation and characterization of the Saccharomyces cerevisiae EKI1 gene encoding ethanolamine kinase."
    Kim K., Kim K.-H., Storey M.K., Voelker D.R., Carman G.M.
    J. Biol. Chem. 274:14857-14866(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEKI1_YEAST
AccessioniPrimary (citable) accession number: Q03764
Secondary accession number(s): D6VSD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3420 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.