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Q03751

- CSP_DROME

UniProt

Q03751 - CSP_DROME

Protein

Cysteine string protein

Gene

Csp

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    May have an important role in presynaptic function.3 Publications

    GO - Biological processi

    1. brain morphogenesis Source: FlyBase
    2. exocytosis Source: FlyBase
    3. locomotion involved in locomotory behavior Source: FlyBase
    4. neurotransmitter secretion Source: FlyBase
    5. protein folding Source: FlyBase
    6. startle response Source: FlyBase
    7. synaptic vesicle exocytosis Source: FlyBase
    8. synaptic vesicle uncoating Source: FlyBase
    9. vesicle-mediated transport Source: FlyBase

    Keywords - Molecular functioni

    Chaperone

    Enzyme and pathway databases

    ReactomeiREACT_180323. GABA synthesis, release, reuptake and degradation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine string protein
    Gene namesi
    Name:Csp
    ORF Names:CG6395
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0004179. Csp.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: FlyBase
    2. synaptic vesicle Source: FlyBase
    3. terminal bouton Source: FlyBase
    4. type Ib terminal bouton Source: FlyBase
    5. type Is terminal bouton Source: FlyBase

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 249249Cysteine string proteinPRO_0000071075Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei12 – 121Phosphoserine1 Publication
    Modified residuei13 – 131Phosphothreonine1 Publication
    Modified residuei14 – 141Phosphoserine1 Publication
    Modified residuei17 – 171Phosphoserine1 Publication
    Modified residuei19 – 191Phosphotyrosine1 Publication

    Post-translational modificationi

    Fatty acylated. Heavily palmitoylated in the cysteine string motif.1 Publication

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiQ03751.
    PRIDEiQ03751.

    Expressioni

    Tissue specificityi

    Expressed in wide range of synaptic terminals: embryonic nervous system, larval neuromuscular junctions, adult visual system (neuropil of optic ganglia and terminal of R1-8 photoreceptors) and thoracic neuromuscular junctions. Also expressed in non-neuronal cells: follicle cells, spermatheca, testis and ejaculatory bulb. Low level of expression is found in many neuronal and non-neuronal tissues.3 Publications

    Gene expression databases

    BgeeiQ03751.

    Interactioni

    Protein-protein interaction databases

    BioGridi65696. 3 interactions.
    IntActiQ03751. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03751.
    SMRiQ03751. Positions 8-102.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 8470JPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi121 – 13111Poly-CysAdd
    BLAST

    Sequence similaritiesi

    Contains 1 J domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0484.
    InParanoidiQ03751.
    KOiK09525.
    OrthoDBiEOG7WHHBD.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    InterProiIPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    [Graphical view]
    PfamiPF00226. DnaJ. 1 hit.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    PROSITEiPS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform CSP1 (identifier: Q03751-1) [UniParc]FASTAAdd to Basket

    Also known as: CSP32, B

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSAPGMDKRK LSTSGDSLYE ILGLPKTATG DDIKKTYRKL ALKYHPDKNP    50
    DNVDAADKFK EVNRAHSILS NQTKRNIYDN YGSLGLYIAE QFGEENVNAY 100
    FVVTSPAVKA VVICCAVITG CCCCCCCCCC CNFCCGKFKP PVNESHDQYS 150
    HLNRPDGNRE GNDMPTHLGQ PPRLEDVDLD DVNLGAGGAP VTSQPREQAG 200
    GQPVFAMPPP SGAVGVNPFT GAPVAANENT SLNTTEQTTY TPDMVNQKY 249
    Length:249
    Mass (Da):26,896
    Last modified:October 1, 1996 - v1
    Checksum:i3EF97C3BF2553EB8
    GO
    Isoform CSP2 (identifier: Q03751-2) [UniParc]FASTAAdd to Basket

    Also known as: C

    The sequence of this isoform differs from the canonical sequence as follows:
         154-174: Missing.

    Show »
    Length:228
    Mass (Da):24,557
    Checksum:iC0F94C47CCA55BE5
    GO
    Isoform CSP3 (identifier: Q03751-3) [UniParc]FASTAAdd to Basket

    Also known as: CSP29, A

    The sequence of this isoform differs from the canonical sequence as follows:
         154-174: Missing.
         243-249: DMVNQKY → GI

    Show »
    Length:223
    Mass (Da):23,848
    Checksum:iB39935CBBB29D9D1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti71 – 711N → D in AAA28432. (PubMed:2129171)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei154 – 17421Missing in isoform CSP3 and isoform CSP2. 2 PublicationsVSP_001293Add
    BLAST
    Alternative sequencei243 – 2497DMVNQKY → GI in isoform CSP3. 2 PublicationsVSP_001294

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63421 mRNA. Translation: AAA28432.1.
    M63008 mRNA. Translation: AAA28431.1.
    AF057167 Genomic DNA. Translation: AAD09428.1.
    AF057167 Genomic DNA. Translation: AAD09430.1.
    AF057167 Genomic DNA. Translation: AAD09431.1.
    AE014296 Genomic DNA. Translation: AAF51816.1.
    AE014296 Genomic DNA. Translation: AAF51817.1.
    AE014296 Genomic DNA. Translation: AAN12195.2.
    AY059457 mRNA. Translation: AAL13363.1. Sequence problems.
    RefSeqiNP_524213.1. NM_079489.3.
    NP_730713.1. NM_168949.2.
    NP_730714.2. NM_168950.4.
    UniGeneiDm.5140.

    Genome annotation databases

    GeneIDi40459.
    KEGGidme:Dmel_CG6395.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63421 mRNA. Translation: AAA28432.1 .
    M63008 mRNA. Translation: AAA28431.1 .
    AF057167 Genomic DNA. Translation: AAD09428.1 .
    AF057167 Genomic DNA. Translation: AAD09430.1 .
    AF057167 Genomic DNA. Translation: AAD09431.1 .
    AE014296 Genomic DNA. Translation: AAF51816.1 .
    AE014296 Genomic DNA. Translation: AAF51817.1 .
    AE014296 Genomic DNA. Translation: AAN12195.2 .
    AY059457 mRNA. Translation: AAL13363.1 . Sequence problems.
    RefSeqi NP_524213.1. NM_079489.3.
    NP_730713.1. NM_168949.2.
    NP_730714.2. NM_168950.4.
    UniGenei Dm.5140.

    3D structure databases

    ProteinModelPortali Q03751.
    SMRi Q03751. Positions 8-102.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 65696. 3 interactions.
    IntActi Q03751. 2 interactions.

    Proteomic databases

    PaxDbi Q03751.
    PRIDEi Q03751.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 40459.
    KEGGi dme:Dmel_CG6395.

    Organism-specific databases

    CTDi 40459.
    FlyBasei FBgn0004179. Csp.

    Phylogenomic databases

    eggNOGi COG0484.
    InParanoidi Q03751.
    KOi K09525.
    OrthoDBi EOG7WHHBD.

    Enzyme and pathway databases

    Reactomei REACT_180323. GABA synthesis, release, reuptake and degradation.

    Miscellaneous databases

    ChiTaRSi Csp. drosophila.
    GenomeRNAii 40459.
    NextBioi 818882.

    Gene expression databases

    Bgeei Q03751.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    InterProi IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    [Graphical view ]
    Pfami PF00226. DnaJ. 1 hit.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    PROSITEi PS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A cysteine-string protein is expressed in retina and brain of Drosophila."
      Zinsmaier K.E., Hofbauer A., Heimbeck G., Pflugfelder G.O., Buchner S., Buchner E.
      J. Neurogenet. 7:15-29(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS CSP1 AND CSP3), FUNCTION, TISSUE SPECIFICITY.
    2. "Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis."
      Eberle K.K., Zinsmaier K.E., Buchner S., Gruhn M., Jenni M., Arnold C., Leibold C., Reisch D., Walter N., Hafen E., Hofbauer A., Pflugfelder G.O., Buchner E.
      Cell Tissue Res. 294:203-217(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS CSP1; CSP2 AND CSP3), FUNCTION, TISSUE SPECIFICITY.
      Strain: Berlin.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 154-249 (ISOFORM CSP3).
      Strain: Berkeley.
      Tissue: Embryo.
    6. "Paralysis and early death in cysteine string protein mutants of Drosophila."
      Zinsmaier K.E., Eberle K.K., Buchner E., Walter N., Benzer S.
      Science 263:977-980(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
      Strain: Berlin.
    7. "Association of Drosophila cysteine string proteins with membranes."
      van de Goor J., Kelly R.B.
      FEBS Lett. 380:251-256(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION.
    8. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; THR-13; SER-14; SER-17 AND TYR-19, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiCSP_DROME
    AccessioniPrimary (citable) accession number: Q03751
    Secondary accession number(s): O61664
    , O61665, Q95TD7, Q9VNV1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3