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Q03751

- CSP_DROME

UniProt

Q03751 - CSP_DROME

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Protein

Cysteine string protein

Gene
Csp, CG6395
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May have an important role in presynaptic function.3 Publications

GO - Biological processi

  1. brain morphogenesis Source: FlyBase
  2. exocytosis Source: FlyBase
  3. locomotion involved in locomotory behavior Source: FlyBase
  4. neurotransmitter secretion Source: FlyBase
  5. protein folding Source: FlyBase
  6. startle response Source: FlyBase
  7. synaptic vesicle exocytosis Source: FlyBase
  8. synaptic vesicle uncoating Source: FlyBase
  9. vesicle-mediated transport Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

ReactomeiREACT_180323. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine string protein
Gene namesi
Name:Csp
ORF Names:CG6395
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004179. Csp.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: FlyBase
  2. synaptic vesicle Source: FlyBase
  3. terminal bouton Source: FlyBase
  4. type Ib terminal bouton Source: FlyBase
  5. type Is terminal bouton Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Cysteine string proteinPRO_0000071075Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121Phosphoserine1 Publication
Modified residuei13 – 131Phosphothreonine1 Publication
Modified residuei14 – 141Phosphoserine1 Publication
Modified residuei17 – 171Phosphoserine1 Publication
Modified residuei19 – 191Phosphotyrosine1 Publication

Post-translational modificationi

Fatty acylated. Heavily palmitoylated in the cysteine string motif.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ03751.
PRIDEiQ03751.

Expressioni

Tissue specificityi

Expressed in wide range of synaptic terminals: embryonic nervous system, larval neuromuscular junctions, adult visual system (neuropil of optic ganglia and terminal of R1-8 photoreceptors) and thoracic neuromuscular junctions. Also expressed in non-neuronal cells: follicle cells, spermatheca, testis and ejaculatory bulb. Low level of expression is found in many neuronal and non-neuronal tissues.3 Publications

Gene expression databases

BgeeiQ03751.

Interactioni

Protein-protein interaction databases

BioGridi65696. 3 interactions.
IntActiQ03751. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ03751.
SMRiQ03751. Positions 8-102.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 8470JAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi121 – 13111Poly-CysAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.

Phylogenomic databases

eggNOGiCOG0484.
InParanoidiQ03751.
KOiK09525.
OrthoDBiEOG7WHHBD.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform CSP1 (identifier: Q03751-1) [UniParc]FASTAAdd to Basket

Also known as: CSP32, B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSAPGMDKRK LSTSGDSLYE ILGLPKTATG DDIKKTYRKL ALKYHPDKNP    50
DNVDAADKFK EVNRAHSILS NQTKRNIYDN YGSLGLYIAE QFGEENVNAY 100
FVVTSPAVKA VVICCAVITG CCCCCCCCCC CNFCCGKFKP PVNESHDQYS 150
HLNRPDGNRE GNDMPTHLGQ PPRLEDVDLD DVNLGAGGAP VTSQPREQAG 200
GQPVFAMPPP SGAVGVNPFT GAPVAANENT SLNTTEQTTY TPDMVNQKY 249
Length:249
Mass (Da):26,896
Last modified:October 1, 1996 - v1
Checksum:i3EF97C3BF2553EB8
GO
Isoform CSP2 (identifier: Q03751-2) [UniParc]FASTAAdd to Basket

Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     154-174: Missing.

Show »
Length:228
Mass (Da):24,557
Checksum:iC0F94C47CCA55BE5
GO
Isoform CSP3 (identifier: Q03751-3) [UniParc]FASTAAdd to Basket

Also known as: CSP29, A

The sequence of this isoform differs from the canonical sequence as follows:
     154-174: Missing.
     243-249: DMVNQKY → GI

Show »
Length:223
Mass (Da):23,848
Checksum:iB39935CBBB29D9D1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei154 – 17421Missing in isoform CSP3 and isoform CSP2. VSP_001293Add
BLAST
Alternative sequencei243 – 2497DMVNQKY → GI in isoform CSP3. VSP_001294

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711N → D in AAA28432. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63421 mRNA. Translation: AAA28432.1.
M63008 mRNA. Translation: AAA28431.1.
AF057167 Genomic DNA. Translation: AAD09428.1.
AF057167 Genomic DNA. Translation: AAD09430.1.
AF057167 Genomic DNA. Translation: AAD09431.1.
AE014296 Genomic DNA. Translation: AAF51816.1.
AE014296 Genomic DNA. Translation: AAF51817.1.
AE014296 Genomic DNA. Translation: AAN12195.2.
AY059457 mRNA. Translation: AAL13363.1. Sequence problems.
RefSeqiNP_524213.1. NM_079489.3.
NP_730713.1. NM_168949.2.
NP_730714.2. NM_168950.4.
UniGeneiDm.5140.

Genome annotation databases

GeneIDi40459.
KEGGidme:Dmel_CG6395.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63421 mRNA. Translation: AAA28432.1 .
M63008 mRNA. Translation: AAA28431.1 .
AF057167 Genomic DNA. Translation: AAD09428.1 .
AF057167 Genomic DNA. Translation: AAD09430.1 .
AF057167 Genomic DNA. Translation: AAD09431.1 .
AE014296 Genomic DNA. Translation: AAF51816.1 .
AE014296 Genomic DNA. Translation: AAF51817.1 .
AE014296 Genomic DNA. Translation: AAN12195.2 .
AY059457 mRNA. Translation: AAL13363.1 . Sequence problems.
RefSeqi NP_524213.1. NM_079489.3.
NP_730713.1. NM_168949.2.
NP_730714.2. NM_168950.4.
UniGenei Dm.5140.

3D structure databases

ProteinModelPortali Q03751.
SMRi Q03751. Positions 8-102.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 65696. 3 interactions.
IntActi Q03751. 2 interactions.

Proteomic databases

PaxDbi Q03751.
PRIDEi Q03751.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 40459.
KEGGi dme:Dmel_CG6395.

Organism-specific databases

CTDi 40459.
FlyBasei FBgn0004179. Csp.

Phylogenomic databases

eggNOGi COG0484.
InParanoidi Q03751.
KOi K09525.
OrthoDBi EOG7WHHBD.

Enzyme and pathway databases

Reactomei REACT_180323. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

ChiTaRSi Csp. drosophila.
GenomeRNAii 40459.
NextBioi 818882.

Gene expression databases

Bgeei Q03751.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
InterProi IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view ]
Pfami PF00226. DnaJ. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A cysteine-string protein is expressed in retina and brain of Drosophila."
    Zinsmaier K.E., Hofbauer A., Heimbeck G., Pflugfelder G.O., Buchner S., Buchner E.
    J. Neurogenet. 7:15-29(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS CSP1 AND CSP3), FUNCTION, TISSUE SPECIFICITY.
  2. "Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis."
    Eberle K.K., Zinsmaier K.E., Buchner S., Gruhn M., Jenni M., Arnold C., Leibold C., Reisch D., Walter N., Hafen E., Hofbauer A., Pflugfelder G.O., Buchner E.
    Cell Tissue Res. 294:203-217(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS CSP1; CSP2 AND CSP3), FUNCTION, TISSUE SPECIFICITY.
    Strain: Berlin.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 154-249 (ISOFORM CSP3).
    Strain: Berkeley.
    Tissue: Embryo.
  6. "Paralysis and early death in cysteine string protein mutants of Drosophila."
    Zinsmaier K.E., Eberle K.K., Buchner E., Walter N., Benzer S.
    Science 263:977-980(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
    Strain: Berlin.
  7. "Association of Drosophila cysteine string proteins with membranes."
    van de Goor J., Kelly R.B.
    FEBS Lett. 380:251-256(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION.
  8. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; THR-13; SER-14; SER-17 AND TYR-19, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiCSP_DROME
AccessioniPrimary (citable) accession number: Q03751
Secondary accession number(s): O61664
, O61665, Q95TD7, Q9VNV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi