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Q03750

- TAF8_YEAST

UniProt

Q03750 - TAF8_YEAST

Protein

Transcription initiation factor TFIID subunit 8

Gene

TAF8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.6 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    3. transcription from RNA polymerase II promoter Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32696-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor TFIID subunit 8
    Alternative name(s):
    TAFII-65
    TBP-associated factor 65 kDa
    TBP-associated factor 8
    Gene namesi
    Name:TAF8
    Synonyms:TAF65
    Ordered Locus Names:YML114C
    ORF Names:YM8339.05C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML114c.
    SGDiS000004582. TAF8.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. transcription factor TFIID complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 510510Transcription initiation factor TFIID subunit 8PRO_0000118887Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei269 – 2691Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ03750.
    PaxDbiQ03750.
    PeptideAtlasiQ03750.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03750.

    Interactioni

    Subunit structurei

    In TFIID, TAF8 heterodimerizes with TAF10. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TAF10Q120307EBI-27947,EBI-18889

    Protein-protein interaction databases

    BioGridi35090. 179 interactions.
    DIPiDIP-1954N.
    IntActiQ03750. 21 interactions.
    MINTiMINT-405858.
    STRINGi4932.YML114C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03750.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 10367Histone-foldAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili142 – 16524Sequence AnalysisAdd
    BLAST
    Coiled coili346 – 38338Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TAF8 family.Curated
    Contains 1 histone-fold domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG267301.
    KOiK14649.
    OMAiDENEMSS.
    OrthoDBiEOG7KH9TV.

    Family and domain databases

    InterProiIPR019473. TFIID_su8_C.
    [Graphical view]
    PfamiPF10406. TAF8_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03750-1 [UniParc]FASTAAdd to Basket

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    MTSKTSESGT GTQSTIVQLR NLPDLTEISH LEIDAPVVEI LKKTVLFQLN    50
    SLNICISNFA LDELVNLVTV QMDGMFRNLH NLTLLQRRSQ ASQADLKLLL 100
    REFNLDAPSL YQQFQASEFI KSKHSTEYEK LMSWSSLAAL PHNEEDEEDE 150
    LNNIEEQQNE INVLLPPSNP LEKQIPSWLP NFPPDHTYKF TPEFNHPITD 200
    LKTIKKEIVK ESQESEKALL NLNKSLSHIS SASNTPQPPG LDDEDAIEQQ 250
    LEIWGNALEE RKPTITEKSF NENNIEQYAK YRVELARERV TKFEVNQLKR 300
    TKNPFLKISE TLYLPESPHQ SHKTIQKTIE LQFRKSMTLF MHNLPKVQKL 350
    KKEKIRMAKE ERAKSLKRRQ EELISQRTKR EQDEGHDLEL LLNNEHARDA 400
    ADDTTTPNAL NNSTIVINTN AEDEDDDINL FGILGSSEDE NEMSSMPAEN 450
    LVAESEPPTM TAQDTTNTTP VAHNTTNIDA TTSHSPHSTP NENAPTSPPA 500
    DIATDHDITM 510
    Length:510
    Mass (Da):57,986
    Last modified:November 1, 1997 - v1
    Checksum:iA6393C86D0E890CE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49210 Genomic DNA. Translation: CAA89104.1.
    BK006946 Genomic DNA. Translation: DAA09784.1.
    PIRiS53958.
    RefSeqiNP_013593.1. NM_001182476.1.

    Genome annotation databases

    EnsemblFungiiYML114C; YML114C; YML114C.
    GeneIDi854926.
    KEGGisce:YML114C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49210 Genomic DNA. Translation: CAA89104.1 .
    BK006946 Genomic DNA. Translation: DAA09784.1 .
    PIRi S53958.
    RefSeqi NP_013593.1. NM_001182476.1.

    3D structure databases

    ProteinModelPortali Q03750.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35090. 179 interactions.
    DIPi DIP-1954N.
    IntActi Q03750. 21 interactions.
    MINTi MINT-405858.
    STRINGi 4932.YML114C.

    Proteomic databases

    MaxQBi Q03750.
    PaxDbi Q03750.
    PeptideAtlasi Q03750.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML114C ; YML114C ; YML114C .
    GeneIDi 854926.
    KEGGi sce:YML114C.

    Organism-specific databases

    CYGDi YML114c.
    SGDi S000004582. TAF8.

    Phylogenomic databases

    eggNOGi NOG267301.
    KOi K14649.
    OMAi DENEMSS.
    OrthoDBi EOG7KH9TV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32696-MONOMER.

    Miscellaneous databases

    NextBioi 977947.

    Gene expression databases

    Genevestigatori Q03750.

    Family and domain databases

    InterProi IPR019473. TFIID_su8_C.
    [Graphical view ]
    Pfami PF10406. TAF8_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family."
      Birck C., Poch O., Romier C., Ruff M., Mengus G., Lavigne A.C., Davidson I., Moras D.
      Cell 94:239-249(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TAF-TAF INTERACTION THROUGH HISTONE-FOLD DOMAIN.
    4. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
      Sanders S.L., Weil P.A.
      J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    5. "Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7."
      Gangloff Y.G., Sanders S.L., Romier C., Kirschner D.B., Weil P.A., Tora L., Davidson I.
      Mol. Cell. Biol. 21:1841-1853(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION IN TFIID AND SAGA.
    6. "The histone fold is a key structural motif of transcription factor TFIID."
      Gangloff Y.G., Romier C., Thuault S., Werten S., Davidson I.
      Trends Biochem. Sci. 26:250-257(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, HISTONE-FOLD DOMAIN CHARACTERIZATION.
    7. "Molecular characterization of Saccharomyces cerevisiae TFIID."
      Sanders S.L., Garbett K.A., Weil P.A.
      Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TFIID STOICHIOMETRY.
    8. "Multi-protein complexes in eukaryotic gene transcription."
      Martinez E.
      Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTAF8_YEAST
    AccessioniPrimary (citable) accession number: Q03750
    Secondary accession number(s): D6W0H0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4684 (+/-522) molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3