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Q03750

- TAF8_YEAST

UniProt

Q03750 - TAF8_YEAST

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Protein
Transcription initiation factor TFIID subunit 8
Gene
TAF8, TAF65, YML114C, YM8339.05C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.6 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32696-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 8
Alternative name(s):
TAFII-65
TBP-associated factor 65 kDa
TBP-associated factor 8
Gene namesi
Name:TAF8
Synonyms:TAF65
Ordered Locus Names:YML114C
ORF Names:YM8339.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML114c.
SGDiS000004582. TAF8.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. transcription factor TFIID complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 510510Transcription initiation factor TFIID subunit 8
PRO_0000118887Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei269 – 2691Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03750.
PaxDbiQ03750.
PeptideAtlasiQ03750.

Expressioni

Gene expression databases

GenevestigatoriQ03750.

Interactioni

Subunit structurei

In TFIID, TAF8 heterodimerizes with TAF10. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TAF10Q120307EBI-27947,EBI-18889

Protein-protein interaction databases

BioGridi35090. 179 interactions.
DIPiDIP-1954N.
IntActiQ03750. 21 interactions.
MINTiMINT-405858.
STRINGi4932.YML114C.

Structurei

3D structure databases

ProteinModelPortaliQ03750.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 10367Histone-fold
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili142 – 16524 Reviewed prediction
Add
BLAST
Coiled coili346 – 38338 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the TAF8 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG267301.
KOiK14649.
OMAiDENEMSS.
OrthoDBiEOG7KH9TV.

Family and domain databases

InterProiIPR019473. TFIID_su8_C.
[Graphical view]
PfamiPF10406. TAF8_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03750-1 [UniParc]FASTAAdd to Basket

« Hide

MTSKTSESGT GTQSTIVQLR NLPDLTEISH LEIDAPVVEI LKKTVLFQLN    50
SLNICISNFA LDELVNLVTV QMDGMFRNLH NLTLLQRRSQ ASQADLKLLL 100
REFNLDAPSL YQQFQASEFI KSKHSTEYEK LMSWSSLAAL PHNEEDEEDE 150
LNNIEEQQNE INVLLPPSNP LEKQIPSWLP NFPPDHTYKF TPEFNHPITD 200
LKTIKKEIVK ESQESEKALL NLNKSLSHIS SASNTPQPPG LDDEDAIEQQ 250
LEIWGNALEE RKPTITEKSF NENNIEQYAK YRVELARERV TKFEVNQLKR 300
TKNPFLKISE TLYLPESPHQ SHKTIQKTIE LQFRKSMTLF MHNLPKVQKL 350
KKEKIRMAKE ERAKSLKRRQ EELISQRTKR EQDEGHDLEL LLNNEHARDA 400
ADDTTTPNAL NNSTIVINTN AEDEDDDINL FGILGSSEDE NEMSSMPAEN 450
LVAESEPPTM TAQDTTNTTP VAHNTTNIDA TTSHSPHSTP NENAPTSPPA 500
DIATDHDITM 510
Length:510
Mass (Da):57,986
Last modified:November 1, 1997 - v1
Checksum:iA6393C86D0E890CE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49210 Genomic DNA. Translation: CAA89104.1.
BK006946 Genomic DNA. Translation: DAA09784.1.
PIRiS53958.
RefSeqiNP_013593.1. NM_001182476.1.

Genome annotation databases

EnsemblFungiiYML114C; YML114C; YML114C.
GeneIDi854926.
KEGGisce:YML114C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49210 Genomic DNA. Translation: CAA89104.1 .
BK006946 Genomic DNA. Translation: DAA09784.1 .
PIRi S53958.
RefSeqi NP_013593.1. NM_001182476.1.

3D structure databases

ProteinModelPortali Q03750.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35090. 179 interactions.
DIPi DIP-1954N.
IntActi Q03750. 21 interactions.
MINTi MINT-405858.
STRINGi 4932.YML114C.

Proteomic databases

MaxQBi Q03750.
PaxDbi Q03750.
PeptideAtlasi Q03750.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML114C ; YML114C ; YML114C .
GeneIDi 854926.
KEGGi sce:YML114C.

Organism-specific databases

CYGDi YML114c.
SGDi S000004582. TAF8.

Phylogenomic databases

eggNOGi NOG267301.
KOi K14649.
OMAi DENEMSS.
OrthoDBi EOG7KH9TV.

Enzyme and pathway databases

BioCyci YEAST:G3O-32696-MONOMER.

Miscellaneous databases

NextBioi 977947.

Gene expression databases

Genevestigatori Q03750.

Family and domain databases

InterProi IPR019473. TFIID_su8_C.
[Graphical view ]
Pfami PF10406. TAF8_C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family."
    Birck C., Poch O., Romier C., Ruff M., Mengus G., Lavigne A.C., Davidson I., Moras D.
    Cell 94:239-249(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TAF-TAF INTERACTION THROUGH HISTONE-FOLD DOMAIN.
  4. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
    Sanders S.L., Weil P.A.
    J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  5. "Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7."
    Gangloff Y.G., Sanders S.L., Romier C., Kirschner D.B., Weil P.A., Tora L., Davidson I.
    Mol. Cell. Biol. 21:1841-1853(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION IN TFIID AND SAGA.
  6. "The histone fold is a key structural motif of transcription factor TFIID."
    Gangloff Y.G., Romier C., Thuault S., Werten S., Davidson I.
    Trends Biochem. Sci. 26:250-257(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, HISTONE-FOLD DOMAIN CHARACTERIZATION.
  7. "Molecular characterization of Saccharomyces cerevisiae TFIID."
    Sanders S.L., Garbett K.A., Weil P.A.
    Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TFIID STOICHIOMETRY.
  8. "Multi-protein complexes in eukaryotic gene transcription."
    Martinez E.
    Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTAF8_YEAST
AccessioniPrimary (citable) accession number: Q03750
Secondary accession number(s): D6W0H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4684 (+/-522) molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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