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Q03735 (NAB6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-binding protein NAB6
Alternative name(s):
Nucleic acid-binding protein 6
Gene names
Name:NAB6
Ordered Locus Names:YML117W
ORF Names:YM8339.02
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length1134 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding protein that associates with mRNAs encoding cell wall proteins. Ref.8

Subcellular location

Cytoplasm Ref.3.

Induction

Expression down-regulated by cAMP. Ref.5

Disruption phenotype

Displays increased sensitivity to some cell wall disrupting agents including sodium dodecyl sulfate (SDS) and calcofluor white (CFW). Ref.5

Miscellaneous

Present with 1540 molecules/cell in log phase SD medium. Ref.4

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandRNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from direct assay Ref.3. Source: SGD

   Molecular functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11341134RNA-binding protein NAB6
PRO_0000082034

Regions

Domain653 – 72674RRM

Amino acid modifications

Modified residue4451Phosphoserine Ref.7
Modified residue4481Phosphoserine Ref.7
Modified residue4491Phosphoserine Ref.7
Modified residue4641Phosphoserine Ref.6
Modified residue4671Phosphoserine Ref.7
Modified residue4971Phosphoserine Ref.7
Modified residue6141Phosphoserine Ref.7
Modified residue9931Phosphoserine Ref.6 Ref.7
Modified residue10181Phosphoserine Ref.7
Modified residue11251Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q03735 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 47B0FF7C5B98A053

FASTA1,134126,139
        10         20         30         40         50         60 
MSNSNSKKPV ANYAYRQQQD YNGMNAMVGN PMMYHPVDFV NGAGQYGPSQ HPAYYTNSPL 

        70         80         90        100        110        120 
PNIPPTPFDT AYGASLFPSH LLMGSPFVSS PNMQSGYNSA RSSNLKRKAY SRPVSNHNGY 

       130        140        150        160        170        180 
NGNSNSNQNN TNNGMVTPSN YYRMGRNSFS RNNNSTRNVT HNNNKGCDTR NNSGRRTFAR 

       190        200        210        220        230        240 
NNIFDDILPE MLLQRPFCIN YKVLPTGDDA YRTRSLLIEN VDHSIDLHSI VKNFVKSNTL 

       250        260        270        280        290        300 
ESAYLIEGGK SDDSKDVETK NLSILISFLT KGDCLNFYNN ILQRLSEFKT FLKSEALNLK 

       310        320        330        340        350        360 
FVCLNYDPKC LPTFIESEAL TENAEEADIT NGSTMISASL HHNIANKDAT RSIIIEFKSP 

       370        380        390        400        410        420 
VEKSDLFKKK LQFLDRSKNK RYILESIDLV NTDVPSNQFP ENYAVLTFLN ISMAIEVLDY 

       430        440        450        460        470        480 
LKKYSKNLGI SKCFYVSLAP LVVSSARSSV ANIYEGKTST HRLSVPSVTA GNNNDSNNNG 

       490        500        510        520        530        540 
NNNKSNMSGI TTLNNNSSIG VSVYGHSNMS LTSLSSSVSL NEEIDMLATK LQGVELDGTY 

       550        560        570        580        590        600 
LEINYRDYQT PTIEEHSTHL SNVKISKTTE NSRQFSQDIP SPLPLNEHMF MNDSNQSNGA 

       610        620        630        640        650        660 
IIPQQLIATP SPVSPNLQMN QRVLPNPITQ SLEQNFNVSA KVASSMGSDI GNRTIYIGNI 

       670        680        690        700        710        720 
NPRSKAEDIC NVVRGGILQS IKYIPEKKIC FVTFIEAPSA VQFYANSFID PIVLHGNMLR 

       730        740        750        760        770        780 
VGWGHYSGPL PKLISLAVTI GASRNVYVSL PEFAFKEKFI HDPQYKKLHE TLSLPDAEQL 

       790        800        810        820        830        840 
REDFSTYGDI EQINYLSDSH CCWINFMNIS SAISLVEEMN KESTVQNESG EVTLKRATEE 

       850        860        870        880        890        900 
KFGGRYKGLL INYGKDRCGN INKNLIAGKN SRFYKKVKRP SYNIRLSKLE EKRRQNEIDE 

       910        920        930        940        950        960 
KEKAFDKPLN LESLGISLDA HKDNGGGETG TANNTGHENE SELEAENENG NETGSFGGLG 

       970        980        990       1000       1010       1020 
LAVASSDVKR ATSDETDYED IFNKSSGSSD SSSDVEVIMH SPSDPEYALK SQTLRSSSQT 

      1030       1040       1050       1060       1070       1080 
VINSKRPVKI EDEEEAVGMS QLNYRSSLRQ APPRAPSTLS YNHSKNNETP MQDIFTNGET 

      1090       1100       1110       1120       1130 
ANNRKKKRGS FARHRTIPGS DVMAQYLAQV QHSTFMYAAN ILGASAEDNT HPDE

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed: 9169872] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Transcriptome profiling of a Saccharomyces cerevisiae mutant with a constitutively activated Ras/cAMP pathway."
Jones D.L., Petty J., Hoyle D.C., Hayes A., Ragni E., Popolo L., Oliver S.G., Stateva L.I.
Physiol. Genomics 16:107-118(2003) [PubMed: 14570984] [Abstract]
Cited for: INDUCTION, DISRUPTION PHENOTYPE.
[6]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-464 AND SER-993, MASS SPECTROMETRY.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445; SER-448; SER-449; SER-467; SER-497; SER-614; SER-993; SER-1018 AND SER-1125, MASS SPECTROMETRY.
[8]"Diverse RNA-binding proteins interact with functionally related sets of RNAs, suggesting an extensive regulatory system."
Hogan D.J., Riordan D.P., Gerber A.P., Herschlag D., Brown P.O.
PLoS Biol. 6:E255-E255(2008) [PubMed: 18959479] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z49210 Genomic DNA. Translation: CAA89101.1.
BK006946 Genomic DNA. Translation: DAA09781.1.
PIRS53955.
RefSeqNP_013589.1. NM_001182479.1.

3D structure databases

ProteinModelPortalQ03735.
SMRQ03735. Positions 650-725, 775-821.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-2976N.
IntActQ03735. 18 interactions.
MINTMINT-630197.
STRINGQ03735.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML117W; YML117W; YML117W.
GeneID854922.
KEGGsce:YML117W.
NMPDRfig|4932.3.peg.4625.

Organism-specific databases

CYGDYML117w.
SGDS000004585. NAB6.

Phylogenomic databases

eggNOGfuNOG08219.
GeneTreeEFGT00050000001635.
HOGENOMHBG398788.
OMADICNVVR.
OrthoDBEOG4X0R20.

Gene expression databases

ArrayExpressQ03735.
GenevestigatorQ03735.
GermOnlineYML117W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR018885. mRNA-bd_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR018835. RNA-binding_domain_put.
IPR000504. RRM_dom.
[Graphical view]
Gene3DG3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
PfamPF10567. Nab6_mRNP_bdg. 1 hit.
PF10378. RRM. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977938.

Entry information

Entry nameNAB6_YEAST
AccessionPrimary (citable) accession number: Q03735
Secondary accession number(s): D6W0G7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents