ID   YMB8_YEAST              Reviewed;         393 AA.
AC   Q03730; D6VZF6; Q6B2K7;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   27-MAR-2024, entry version 158.
DE   RecName: Full=Uncharacterized vacuolar membrane protein YML018C;
GN   OrderedLocusNames=YML018C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:14562095};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 2010 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the TPT transporter family. {ECO:0000305}.
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DR   EMBL; Z46659; CAA86637.1; -; Genomic_DNA.
DR   EMBL; AY692723; AAT92742.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA09880.1; -; Genomic_DNA.
DR   PIR; S49759; S49759.
DR   RefSeq; NP_013694.1; NM_001182376.1.
DR   AlphaFoldDB; Q03730; -.
DR   SMR; Q03730; -.
DR   BioGRID; 35151; 53.
DR   DIP; DIP-4953N; -.
DR   IntAct; Q03730; 24.
DR   MINT; Q03730; -.
DR   STRING; 4932.YML018C; -.
DR   TCDB; 2.A.7.24.5; the drug/metabolite transporter (dmt) superfamily.
DR   iPTMnet; Q03730; -.
DR   PaxDb; 4932-YML018C; -.
DR   PeptideAtlas; Q03730; -.
DR   EnsemblFungi; YML018C_mRNA; YML018C; YML018C.
DR   GeneID; 854990; -.
DR   KEGG; sce:YML018C; -.
DR   AGR; SGD:S000004480; -.
DR   SGD; S000004480; YML018C.
DR   VEuPathDB; FungiDB:YML018C; -.
DR   eggNOG; KOG2765; Eukaryota.
DR   GeneTree; ENSGT00390000005949; -.
DR   HOGENOM; CLU_026578_1_0_1; -.
DR   InParanoid; Q03730; -.
DR   OMA; MYGVYTI; -.
DR   OrthoDB; 1060790at2759; -.
DR   BioCyc; YEAST:G3O-32622-MONOMER; -.
DR   BioGRID-ORCS; 854990; 1 hit in 10 CRISPR screens.
DR   PRO; PR:Q03730; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; Q03730; Protein.
DR   GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR   PANTHER; PTHR23051:SF0; SOLUTE CARRIER FAMILY 35 MEMBER F5; 1.
DR   PANTHER; PTHR23051; SOLUTE CARRIER FAMILY 35, MEMBER F5; 1.
DR   SUPFAM; SSF103481; Multidrug resistance efflux transporter EmrE; 2.
PE   1: Evidence at protein level;
KW   Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW   Transmembrane helix; Vacuole.
FT   CHAIN           1..393
FT                   /note="Uncharacterized vacuolar membrane protein YML018C"
FT                   /id="PRO_0000203261"
FT   TOPO_DOM        1..17
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        39..46
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        68..132
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        154..156
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        157..176
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        177..182
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        183..200
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        201..219
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        220..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        241..257
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        279..292
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        293..313
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        314..321
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        322..342
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        343..345
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..366
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        367..393
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         93
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950"
FT   CONFLICT        257
FT                   /note="K -> R (in Ref. 3; AAT92742)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   393 AA;  43749 MW;  DEC5C3EEF293F5ED CRC64;
     MVSKDQTSFN KRWTLGLLML GLVIILWVLS SFLINLIFED DSYRKPFFIT YTNTAAFIFY
     LFPTAKAVVV NYKDTGRANV HRELIMEEEG TGSDSNRSVD MTSPLLTNLE AGTHANQKKR
     LTLYETIKLS AEFCILWFTA NLVTNASLAF TSVASQTILS TTSSFFTLFI GAICHVESLS
     KSKVLGSFIS FVGIIMVTKS DSHQRYQRHI ADVSGDDNDA VQVLIGNLLA LAGAVLYGVY
     STLLKREVGD ETRVNMKIFF GFVGLFNLLF LWPSLIVLDF FGWEPFSLPK DPKVVVIIFV
     NCLITFVSDF CWAKAMLLTS PLTVTVGLSI TIPLAMFGDV IFKHKTMSAL YLFGATLILG
     SFFIINKSSE EEHFENSITA SNYESVEVPA ANN
//