Uncharacterized vacuolar membrane protein YML018C - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q03730 (YMB8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Uncharacterized vacuolar membrane protein YML018C

Gene names

Ordered Locus Names:

YML018C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.4.
Miscellaneous	Present with 2010 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TPT transporter family.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular_component	fungal-type vacuole membrane Inferred from direct assay Ref.4 PubMed 22842922. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ATG27	P46989	1	EBI-27739,EBI-26164
COT1	P32798	1	EBI-27739,EBI-5006
FET5	P43561	1	EBI-27739,EBI-6891
FTH1	P38310	1	EBI-27739,EBI-20959
PMC1	P38929	1	EBI-27739,EBI-3097
RBD2	Q12270	1	EBI-27739,EBI-31471
SFT2	P38166	1	EBI-27739,EBI-17046
TVP15	Q03860	1	EBI-27739,EBI-37537

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 393

393

Uncharacterized vacuolar membrane protein YML018C

PRO_0000203261

Regions

Topological domain

1 – 17

Cytoplasmic Potential

Transmembrane

18 – 38

Helical; Potential

Topological domain

39 – 46

Vacuolar Potential

Transmembrane

47 – 67

Helical; Potential

Topological domain

68 – 132

Cytoplasmic Potential

Transmembrane

133 – 153

Helical; Potential

Topological domain

154 – 156

Vacuolar Potential

Transmembrane

157 – 176

Helical; Potential

Topological domain

177 – 182

Cytoplasmic Potential

Transmembrane

183 – 200

Helical; Potential

Topological domain

201 – 219

Vacuolar Potential

Transmembrane

220 – 240

Helical; Potential

Topological domain

241 – 257

Cytoplasmic Potential

Transmembrane

258 – 278

Helical; Potential

Topological domain

279 – 292

Vacuolar Potential

Transmembrane

293 – 313

Helical; Potential

Topological domain

314 – 321

Cytoplasmic Potential

Transmembrane

322 – 342

Helical; Potential

Topological domain

343 – 345

Vacuolar Potential

Transmembrane

346 – 366

Helical; Potential

Topological domain

367 – 393

Cytoplasmic Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Modified residue

103

Phosphoserine Ref.8

Experimental info

Sequence conflict

257

K → R in AAT92742. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q03730 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: DEC5C3EEF293F5ED
Show »

FASTA

393

43,749

        10         20         30         40         50         60 
MVSKDQTSFN KRWTLGLLML GLVIILWVLS SFLINLIFED DSYRKPFFIT YTNTAAFIFY 

        70         80         90        100        110        120 
LFPTAKAVVV NYKDTGRANV HRELIMEEEG TGSDSNRSVD MTSPLLTNLE AGTHANQKKR 

       130        140        150        160        170        180 
LTLYETIKLS AEFCILWFTA NLVTNASLAF TSVASQTILS TTSSFFTLFI GAICHVESLS 

       190        200        210        220        230        240 
KSKVLGSFIS FVGIIMVTKS DSHQRYQRHI ADVSGDDNDA VQVLIGNLLA LAGAVLYGVY 

       250        260        270        280        290        300 
STLLKREVGD ETRVNMKIFF GFVGLFNLLF LWPSLIVLDF FGWEPFSLPK DPKVVVIIFV 

       310        320        330        340        350        360 
NCLITFVSDF CWAKAMLLTS PLTVTVGLSI TIPLAMFGDV IFKHKTMSAL YLFGATLILG 

       370        380        390 
SFFIINKSSE EEHFENSITA SNYESVEVPA ANN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93, MASS SPECTROMETRY. Strain: ADR376.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46659 Genomic DNA. Translation: CAA86637.1. AY692723 Genomic DNA. Translation: AAT92742.1. BK006946 Genomic DNA. Translation: DAA09880.1.
PIR	S49759.
RefSeq	NP_013694.1. NM_001182376.1.
3D structure databases
ProteinModelPortal	Q03730.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4953N.
IntAct	Q03730. 23 interactions.
MINT	MINT-548702.
STRING	4932.YML018C.
Proteomic databases
PaxDb	Q03730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML018C; YML018C; YML018C.
GeneID	854990.
KEGG	sce:YML018C.
Organism-specific databases
CYGD	YML018c.
SGD	S000004480. YML018C.
Phylogenomic databases
eggNOG	COG0697.
GeneTree	ENSGT00390000005949.
HOGENOM	HOG000176279.
KO	K15289.
OMA	YMIRYLL.
OrthoDB	EOG49631H.
Gene expression databases
Genevestigator	Q03730.
GermOnline	YML018C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013657. UAA. [Graphical view]
Pfam	PF08449. UAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978127.

Entry information

Entry name

YMB8_YEAST

Accession

Primary (citable) accession number: Q03730
Secondary accession number(s): D6VZF6, Q6B2K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents