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Q03730 (YMB8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uncharacterized vacuolar membrane protein YML018C
Gene names
Ordered Locus Names:YML018C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Vacuole membrane; Multi-pass membrane protein Ref.4.

Miscellaneous

Present with 2010 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TPT transporter family.

Ontologies

Keywords
   Cellular componentMembrane
Vacuole
   DomainTransmembrane
Transmembrane helix
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtransmembrane transport

Inferred from electronic annotation. Source: InterPro

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

vacuolar membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393Uncharacterized vacuolar membrane protein YML018C
PRO_0000203261

Regions

Topological domain1 – 1717Cytoplasmic Potential
Transmembrane18 – 3821Helical; Potential
Topological domain39 – 468Vacuolar Potential
Transmembrane47 – 6721Helical; Potential
Topological domain68 – 13265Cytoplasmic Potential
Transmembrane133 – 15321Helical; Potential
Topological domain154 – 1563Vacuolar Potential
Transmembrane157 – 17620Helical; Potential
Topological domain177 – 1826Cytoplasmic Potential
Transmembrane183 – 20018Helical; Potential
Topological domain201 – 21919Vacuolar Potential
Transmembrane220 – 24021Helical; Potential
Topological domain241 – 25717Cytoplasmic Potential
Transmembrane258 – 27821Helical; Potential
Topological domain279 – 29214Vacuolar Potential
Transmembrane293 – 31321Helical; Potential
Topological domain314 – 3218Cytoplasmic Potential
Transmembrane322 – 34221Helical; Potential
Topological domain343 – 3453Vacuolar Potential
Transmembrane346 – 36621Helical; Potential
Topological domain367 – 39327Cytoplasmic Potential

Amino acid modifications

Modified residue931Phosphoserine Ref.7

Experimental info

Sequence conflict2571K → R in AAT92742. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q03730 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: DEC5C3EEF293F5ED

FASTA39343,749
        10         20         30         40         50         60 
MVSKDQTSFN KRWTLGLLML GLVIILWVLS SFLINLIFED DSYRKPFFIT YTNTAAFIFY 

        70         80         90        100        110        120 
LFPTAKAVVV NYKDTGRANV HRELIMEEEG TGSDSNRSVD MTSPLLTNLE AGTHANQKKR 

       130        140        150        160        170        180 
LTLYETIKLS AEFCILWFTA NLVTNASLAF TSVASQTILS TTSSFFTLFI GAICHVESLS 

       190        200        210        220        230        240 
KSKVLGSFIS FVGIIMVTKS DSHQRYQRHI ADVSGDDNDA VQVLIGNLLA LAGAVLYGVY 

       250        260        270        280        290        300 
STLLKREVGD ETRVNMKIFF GFVGLFNLLF LWPSLIVLDF FGWEPFSLPK DPKVVVIIFV 

       310        320        330        340        350        360 
NCLITFVSDF CWAKAMLLTS PLTVTVGLSI TIPLAMFGDV IFKHKTMSAL YLFGATLILG 

       370        380        390 
SFFIINKSSE EEHFENSITA SNYESVEVPA ANN 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46659 Genomic DNA. Translation: CAA86637.1.
AY692723 Genomic DNA. Translation: AAT92742.1.
BK006946 Genomic DNA. Translation: DAA09880.1.
PIRS49759.
RefSeqNP_013694.1. NM_001182376.1.

3D structure databases

ProteinModelPortalQ03730.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35151. 14 interactions.
DIPDIP-4953N.
IntActQ03730. 23 interactions.
MINTMINT-548702.
STRING4932.YML018C.

Protein family/group databases

TCDB2.A.7.24.5. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PaxDbQ03730.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML018C; YML018C; YML018C.
GeneID854990.
KEGGsce:YML018C.

Organism-specific databases

CYGDYML018c.
SGDS000004480. YML018C.

Phylogenomic databases

eggNOGCOG0697.
GeneTreeENSGT00390000005949.
HOGENOMHOG000176279.
KOK15289.
OMAFFILHWT.
OrthoDBEOG7QVMD1.

Enzyme and pathway databases

BioCycYEAST:G3O-32622-MONOMER.

Gene expression databases

GenevestigatorQ03730.

Family and domain databases

InterProIPR013657. UAA.
[Graphical view]
PfamPF08449. UAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978127.
PROQ03730.

Entry information

Entry nameYMB8_YEAST
AccessionPrimary (citable) accession number: Q03730
Secondary accession number(s): D6VZF6, Q6B2K7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families