Uncharacterized vacuolar membrane protein YML018C - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot Q03730 (YMB8_YEAST)

Last modified November 24, 2009. Version 69. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Uncharacterized vacuolar membrane protein YML018C

Gene names

Ordered Locus Names:

YML018C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Subcellular location	Vacuole membrane; Multi-pass membrane protein.
Miscellaneous	Present with 2010 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the TPT transporter family.

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Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	fungal-type vacuole membrane Ref.3 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
ATG27	P46989	1	EBI-27739,EBI-26164
COT1	P32798	1	EBI-27739,EBI-5006
FET5	P43561	1	EBI-27739,EBI-6891
FTH1	P38310	1	EBI-27739,EBI-20959
PMC1	P38929	1	EBI-27739,EBI-3097
RBD2	Q12270	1	EBI-27739,EBI-31471
SFT2	P38166	1	EBI-27739,EBI-17046
TVP15	Q03860	1	EBI-27739,EBI-37537

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 393

393

Uncharacterized vacuolar membrane protein YML018C

PRO_0000203261

Regions

Topological domain

1 – 17

Cytoplasmic Potential

Transmembrane

18 – 38

Potential

Topological domain

39 – 46

Vacuolar Potential

Transmembrane

47 – 67

Potential

Topological domain

68 – 132

Cytoplasmic Potential

Transmembrane

133 – 153

Potential

Topological domain

154 – 156

Vacuolar Potential

Transmembrane

157 – 176

Potential

Topological domain

177 – 182

Cytoplasmic Potential

Transmembrane

183 – 200

Potential

Topological domain

201 – 219

Vacuolar Potential

Transmembrane

220 – 240

Potential

Topological domain

241 – 257

Cytoplasmic Potential

Transmembrane

258 – 278

Potential

Topological domain

279 – 292

Vacuolar Potential

Transmembrane

293 – 313

Potential

Topological domain

314 – 321

Cytoplasmic Potential

Transmembrane

322 – 342

Potential

Topological domain

343 – 345

Vacuolar Potential

Transmembrane

346 – 366

Potential

Topological domain

367 – 393

Cytoplasmic Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.6

Modified residue

103

Phosphoserine Ref.7

Experimental info

Sequence conflict

257

K → R in AAT92742. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

Q03730-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: DEC5C3EEF293F5ED
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FASTA

393

43,749

        10         20         30         40         50         60 
MVSKDQTSFN KRWTLGLLML GLVIILWVLS SFLINLIFED DSYRKPFFIT YTNTAAFIFY 

        70         80         90        100        110        120 
LFPTAKAVVV NYKDTGRANV HRELIMEEEG TGSDSNRSVD MTSPLLTNLE AGTHANQKKR 

       130        140        150        160        170        180 
LTLYETIKLS AEFCILWFTA NLVTNASLAF TSVASQTILS TTSSFFTLFI GAICHVESLS 

       190        200        210        220        230        240 
KSKVLGSFIS FVGIIMVTKS DSHQRYQRHI ADVSGDDNDA VQVLIGNLLA LAGAVLYGVY 

       250        260        270        280        290        300 
STLLKREVGD ETRVNMKIFF GFVGLFNLLF LWPSLIVLDF FGWEPFSLPK DPKVVVIIFV 

       310        320        330        340        350        360 
NCLITFVSDF CWAKAMLLTS PLTVTVGLSI TIPLAMFGDV IFKHKTMSAL YLFGATLILG 

       370        380        390 
SFFIINKSSE EEHFENSITA SNYESVEVPA ANN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	Z46659 Genomic DNA. Translation: CAA86637.1. AY692723 Genomic DNA. Translation: AAT92742.1.
PIR	S49759.
RefSeq	NP_013694.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4953N.
IntAct	Q03730. 28 interactions.
STRING	Q03730.
Genome annotation databases
Ensembl	YML018C; YML018C; YML018C; Saccharomyces cerevisiae. [Genome view]
GeneID	854990.
KEGG	sce:YML018C.
NMPDR	fig\|4932.3.peg.4735.
Organism-specific databases
CYGD	YML018c.
SGD	S000004480. YML018C.
Phylogenomic databases
HOGENOM	Q03730.
OMA	KPFFITY
OrthoDB	EOG9869W2
Gene expression databases
ArrayExpress	Q03730.
Genevestigator	Q03730.
GermOnline	YML018C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004853. DUF250. [Graphical view]
Pfam	PF03151. TPT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978127.

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Entry information

Entry name

YMB8_YEAST

Accession

Primary (citable) accession number: Q03730
Secondary accession number(s): Q6B2K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents