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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6

Gene

OST6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Can participate in redox reactions and is able to catalyze dithiol-disulfide exchange reactions with other proteins, albeit with relatively low efficiency. May form transient disulfide bonds with nascent polypeptides in the endoplasmic reticulum and thereby promote efficient glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal oligosaccharyl transferase activity.1 Publication

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Redox potential

E0 is -275 mV.1 Publication

Manual assertion based on experiment ini

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein complex assembly Source: SGD
  • protein N-linked glycosylation via asparagine Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YML019W-MONOMER.
YEAST:YML019W-MONOMER.
BRENDAi2.4.99.18. 984.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6 (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit OST6
Alternative name(s):
Oligosaccharyl transferase 37 kDa subunit
Short name:
OTase 37 kDa subunit
Gene namesi
Name:OST6
Ordered Locus Names:YML019W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML019W.
SGDiS000004481. OST6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 188LumenalSequence analysisAdd BLAST164
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Transmembranei268 – 288HelicalSequence analysisAdd BLAST21
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Topological domaini324 – 332LumenalSequence analysis9

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • oligosaccharyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78C → S: Alters glycosyltransferase efficiency towards a subset of target proteins; when associated with S-81. 1 Publication1
Mutagenesisi81C → S: Alters glycosyltransferase efficiency towards a subset of target proteins; when associated with S-78. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000005809825 – 332Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6Add BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi78 ↔ 81Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ03723.
PRIDEiQ03723.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. May interact directly with OST1, OST2, OST4, STT3, WBP1 and SWP1.1 Publication

Protein-protein interaction databases

BioGridi35150. 86 interactors.
DIPiDIP-3851N.
IntActiQ03723. 3 interactors.
MINTiMINT-509989.

Structurei

Secondary structure

1332
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 34Combined sources4
Beta strandi41 – 44Combined sources4
Turni47 – 49Combined sources3
Helixi50 – 53Combined sources4
Beta strandi61 – 67Combined sources7
Helixi79 – 98Combined sources20
Beta strandi104 – 110Combined sources7
Turni111 – 113Combined sources3
Helixi115 – 120Combined sources6
Beta strandi128 – 132Combined sources5
Helixi137 – 142Combined sources6
Turni145 – 147Combined sources3
Beta strandi151 – 153Combined sources3
Helixi157 – 159Combined sources3
Helixi163 – 174Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G7YX-ray2.21A24-188[»]
3G9BX-ray1.96A24-188[»]
3GA4X-ray1.30A24-188[»]
ProteinModelPortaliQ03723.
SMRiQ03723.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03723.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 162ThioredoxinAdd BLAST128

Sequence similaritiesi

Belongs to the OST3/OST6 family.Curated
Contains 1 thioredoxin domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000115296.
InParanoidiQ03723.
KOiK12669.
OMAiYQFGIEV.
OrthoDBiEOG092C32YT.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR021149. OligosaccharylTrfase_OST3/OST6.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF04756. OST3_OST6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03723-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWCSTYIII WLAIIFHKFQ KSTATASHNI DDILQLKDDT GVITVTADNY
60 70 80 90 100
PLLSRGVPGY FNILYITMRG TNSNGMSCQL CHDFEKTYHA VADVIRSQAP
110 120 130 140 150
QSLNLFFTVD VNEVPQLVKD LKLQNVPHLV VYPPAESNKQ SQFEWKTSPF
160 170 180 190 200
YQYSLVPENA ENTLQFGDFL AKILNISITV PQAFNVQEFV YYFVACMVVF
210 220 230 240 250
IFIKKVILPK VTNKWKLFSM ILSLGILLPS ITGYKFVEMN AIPFIARDAK
260 270 280 290 300
NRIMYFSGGS GWQFGIEIFS VSLMYIVMSA LSVLLIYVPK ISCVSEKMRG
310 320 330
LLSSFLACVL FYFFSYFISC YLIKNPGYPI VF
Length:332
Mass (Da):37,891
Last modified:November 1, 1997 - v1
Checksum:iFC7AE156BF27F1B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33I → T in AAT92993 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08606 Genomic DNA. Translation: CAA69898.1.
Z46659 Genomic DNA. Translation: CAA86636.1.
AY692974 Genomic DNA. Translation: AAT92993.1.
BK006946 Genomic DNA. Translation: DAA09879.1.
PIRiS49758.
RefSeqiNP_013693.1. NM_001182377.1.

Genome annotation databases

EnsemblFungiiYML019W; YML019W; YML019W.
GeneIDi854989.
KEGGisce:YML019W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08606 Genomic DNA. Translation: CAA69898.1.
Z46659 Genomic DNA. Translation: CAA86636.1.
AY692974 Genomic DNA. Translation: AAT92993.1.
BK006946 Genomic DNA. Translation: DAA09879.1.
PIRiS49758.
RefSeqiNP_013693.1. NM_001182377.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G7YX-ray2.21A24-188[»]
3G9BX-ray1.96A24-188[»]
3GA4X-ray1.30A24-188[»]
ProteinModelPortaliQ03723.
SMRiQ03723.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35150. 86 interactors.
DIPiDIP-3851N.
IntActiQ03723. 3 interactors.
MINTiMINT-509989.

Proteomic databases

MaxQBiQ03723.
PRIDEiQ03723.

Protocols and materials databases

DNASUi854989.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML019W; YML019W; YML019W.
GeneIDi854989.
KEGGisce:YML019W.

Organism-specific databases

EuPathDBiFungiDB:YML019W.
SGDiS000004481. OST6.

Phylogenomic databases

HOGENOMiHOG000115296.
InParanoidiQ03723.
KOiK12669.
OMAiYQFGIEV.
OrthoDBiEOG092C32YT.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:YML019W-MONOMER.
YEAST:YML019W-MONOMER.
BRENDAi2.4.99.18. 984.

Miscellaneous databases

EvolutionaryTraceiQ03723.
PROiQ03723.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR021149. OligosaccharylTrfase_OST3/OST6.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF04756. OST3_OST6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOST6_YEAST
AccessioniPrimary (citable) accession number: Q03723
Secondary accession number(s): D6VZF5, E9P904
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1080 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.