Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6

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Reviewed, UniProtKB/Swiss-Prot Q03723 (OST6_YEAST)

Last modified February 9, 2010. Version 75. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6
      Short name=Oligosaccharyl transferase subunit OST6
    EC=2.4.1.119
Alternative name(s):
    Oligosaccharyl transferase 37 kDa subunit
      Short name=OTase 37 kDa subunit

Gene names

Name:	OST6
Ordered Locus Names:	YML019W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyl transferase (OST) complex, which apears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. May interact directly with OST1, OST2, OST4, STT3, WBP1 and SWP1.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Miscellaneous	Present with 1080 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the OST3/OST6 family.

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Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation via asparagine Ref.1 Inferred from genetic interaction. Source: SGD protein complex assembly Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell oligosaccharyltransferase complex Ref.1 Ref.5 Inferred from physical interaction. Source: SGD
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Ref.1 Inferred from physical interaction. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
AGP1	P25376	1	EBI-12696,EBI-2357
ECM4	P36156	1	EBI-12696,EBI-2042717
OST1	P41543	1	EBI-12696,EBI-12651
PMP2	P40975	1	EBI-12696,EBI-2043041
SAC1	P32368	1	EBI-12696,EBI-16210
SPF1	P39986	1	EBI-12696,EBI-3128

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 332

332

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6

PRO_0000058098

Regions

Topological domain

1 – 188

188

Cytoplasmic Potential

Transmembrane

189 – 209

Potential

Transmembrane

217 – 237

Potential

Transmembrane

268 – 288

Potential

Transmembrane

303 – 323

Potential

Topological domain

324 – 332

Cytoplasmic Potential

Secondary structure

332

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q03723-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: FC7AE156BF27F1B5
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FASTA

332

37,891

        10         20         30         40         50         60 
MKWCSTYIII WLAIIFHKFQ KSTATASHNI DDILQLKDDT GVITVTADNY PLLSRGVPGY 

        70         80         90        100        110        120 
FNILYITMRG TNSNGMSCQL CHDFEKTYHA VADVIRSQAP QSLNLFFTVD VNEVPQLVKD 

       130        140        150        160        170        180 
LKLQNVPHLV VYPPAESNKQ SQFEWKTSPF YQYSLVPENA ENTLQFGDFL AKILNISITV 

       190        200        210        220        230        240 
PQAFNVQEFV YYFVACMVVF IFIKKVILPK VTNKWKLFSM ILSLGILLPS ITGYKFVEMN 

       250        260        270        280        290        300 
AIPFIARDAK NRIMYFSGGS GWQFGIEIFS VSLMYIVMSA LSVLLIYVPK ISCVSEKMRG 

       310        320        330 
LLSSFLACVL FYFFSYFISC YLIKNPGYPI VF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The oligosaccharyltransferase complex from Saccharomyces cerevisiae. Isolation of the OST6 gene, its synthetic interaction with OST3, and analysis of the native complex." Knauer R., Lehle L. J. Biol. Chem. 274:17249-17256(1999) [PubMed: 10358084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The oligosaccharyltransferase complex from yeast." Knauer R., Lehle L. Biochim. Biophys. Acta 1426:259-273(1999) [PubMed: 9878773] [Abstract] Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit." Schwarz M., Knauer R., Lehle L. FEBS Lett. 579:6564-6568(2005) [PubMed: 16297388] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[6]	"The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast." Spirig U., Bodmer D., Wacker M., Burda P., Aebi M. Glycobiology 15:1396-1406(2005) [PubMed: 16096346] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[7]	"Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons." Yan A., Lennarz W.J. Glycobiology 15:1407-1415(2005) [PubMed: 16096345] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[8]	"Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions." Yan A., Wu E., Lennarz W.J. Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed: 15886282] [Abstract] Cited for: TOPOLOGY, INTERACTION WITH OST1; OST2; OST4; STT3; WBP1 AND SWP1.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y08606 Genomic DNA. Translation: CAA69898.1.
Z46659 Genomic DNA. Translation: CAA86636.1.

PIR

S49758.

RefSeq

NP_013693.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3G7Y	X-ray	2.21	A	24-188	[»]
3G9B	X-ray	1.96	A	24-188	[»]
3GA4	X-ray	1.30	A	24-188	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3851N.

IntAct

Q03723. 13 interactions.

STRING

Q03723.

Genome annotation databases

Ensembl

YML019W; YML019W; YML019W; Saccharomyces cerevisiae. [Genome view]

GeneID

854989.

KEGG

sce:YML019W.

NMPDR

fig|4932.3.peg.4734.

Organism-specific databases

CYGD

YML019w.

SGD

S000004481. OST6.

Phylogenomic databases

eggNOG

HOGENOM

OMA

OrthoDB

PhylomeDB

Q03723.

Enzyme and pathway databases

BRENDA

2.4.1.119. 250.

Gene expression databases

ArrayExpress

Q03723.

Genevestigator

Q03723.

GermOnline

YML019W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR006844. Mg_transporter-1.
[Graphical view]

PANTHER

PTHR12692. OST3_OST6. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

978124.

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Entry information

Entry name

OST6_YEAST

Accession

Primary (citable) accession number: Q03723

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents