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Q03717

- KCNB1_MOUSE

UniProt

Q03717 - KCNB1_MOUSE

Protein

Potassium voltage-gated channel subfamily B member 1

Gene

Kcnb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient.

    GO - Molecular functioni

    1. delayed rectifier potassium channel activity Source: RefGenome
    2. outward rectifier potassium channel activity Source: Ensembl
    3. protein binding Source: IntAct
    4. voltage-gated potassium channel activity Source: MGI

    GO - Biological processi

    1. potassium ion transport Source: MGI
    2. protein homooligomerization Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199077. Voltage gated Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily B member 1
    Alternative name(s):
    Voltage-gated potassium channel subunit Kv2.1
    mShab
    Gene namesi
    Name:Kcnb1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:96666. Kcnb1.

    Subcellular locationi

    GO - Cellular componenti

    1. dendrite Source: Ensembl
    2. dendrite membrane Source: Ensembl
    3. neuronal cell body membrane Source: Ensembl
    4. postsynaptic membrane Source: Ensembl
    5. voltage-gated potassium channel complex Source: InterPro

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 857857Potassium voltage-gated channel subfamily B member 1PRO_0000054043Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei15 – 151PhosphoserineBy similarity
    Modified residuei128 – 1281Phosphotyrosine; by SrcBy similarity
    Modified residuei457 – 4571PhosphoserineBy similarity
    Modified residuei484 – 4841PhosphoserineBy similarity
    Modified residuei496 – 4961PhosphoserineBy similarity
    Modified residuei503 – 5031PhosphoserineBy similarity
    Modified residuei520 – 5201PhosphoserineBy similarity
    Modified residuei541 – 5411PhosphoserineBy similarity
    Modified residuei567 – 5671PhosphoserineBy similarity
    Modified residuei590 – 5901PhosphoserineBy similarity
    Modified residuei607 – 6071PhosphoserineBy similarity
    Modified residuei655 – 6551Phosphoserine1 Publication
    Modified residuei719 – 7191PhosphoserineBy similarity
    Modified residuei771 – 7711PhosphoserineBy similarity
    Modified residuei799 – 7991PhosphoserineBy similarity
    Modified residuei804 – 8041PhosphoserineBy similarity
    Modified residuei836 – 8361PhosphothreonineBy similarity

    Post-translational modificationi

    Highly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-655 and Ser-719 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/ dephosphorylation activities. Tyr-128 can be dephosphorylated by PTPalpha and cyt-PTPepsilon. Phosphorylation levels on Ser-607 are supersensitive to neuronal activity. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5. Levels then increase to reach adult levels by P14. Phosphorylation levels on Ser-564 and Ser-607 are greatly reduced during seizures, by 40% and 85% respectively.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ03717.
    PRIDEiQ03717.

    PTM databases

    PhosphoSiteiQ03717.

    Expressioni

    Gene expression databases

    ArrayExpressiQ03717.
    BgeeiQ03717.
    GenevestigatoriQ03717.

    Interactioni

    Subunit structurei

    Heteromultimer with KCNG2, KCNG3, KCNG4, KCNS1, KCNS2, KCNS3 and KCNV2.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Amigo1Q80ZD84EBI-7511364,EBI-7511393

    Protein-protein interaction databases

    BioGridi200886. 3 interactions.
    IntActiQ03717. 1 interaction.
    MINTiMINT-8298631.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03717.
    SMRiQ03717. Positions 30-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 185185CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini250 – 25910CytoplasmicSequence Analysis
    Topological domaini316 – 33015CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini414 – 857444CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei365 – 38521Pore-forming; Name=Segment H5Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei186 – 20621Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei229 – 24921Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei295 – 31521Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei331 – 35121Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei393 – 41321Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi377 – 3826Selectivity filterBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi517 – 5204Poly-Ser

    Domaini

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00740000114905.
    HOGENOMiHOG000113206.
    HOVERGENiHBG052225.
    InParanoidiQ8K0D1.
    KOiK04885.
    OMAiCATDFPE.
    OrthoDBiEOG7CRTPP.
    TreeFamiTF313103.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003973. K_chnl_volt-dep_Kv2.
    IPR004350. K_chnl_volt-dep_Kv2.1.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF03521. Kv2channel. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01514. KV21CHANNEL.
    PR01491. KVCHANNEL.
    PR01495. SHABCHANNEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q03717-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAGMTKHGS RSTSSLPPEP MEIVRSKACS RRVRLNVGGL AHEVLWRTLD    50
    RLPRTRLGKL RDCNTHDSLL QVCDDYSLED NEYFFDRHPG AFTSILNFYR 100
    TGRLHMMEEM CALSFSQELD YWGIDEIYLE SCCQARYHQK KEQMNEELKR 150
    EAETLREREG EEFDNTCCAE KRKKLWDLLE KPNSSVAAKI LAIISIMFIV 200
    LSTIALSLNT LPELQSLDEF GQSTDNPQLA HVEAVCIAWF TMEYLLRFLS 250
    SPKKWKFFKG PLNAIDLLAI LPYYVTIFLT ESNKSVLQFQ NVRRVVQIFR 300
    IMRILRILKL ARHSTGLQSL GFTLRRSYNE LGLLILFLAM GIMIFSSLVF 350
    FAEKDEDDTK FKSIPASFWW ATITMTTVGY GDIYPKTLLG KIVGGLCCIA 400
    GVLVIALPIP IIVNNFSEFY KEQKRQEKAI KRREALERAK RNGSIVSMNM 450
    KDAFARSIEM MDIVVEKNGE GVAKKDKVQD NHLSPNKWKW TKRALSETSS 500
    SKSFETKEQG SPEKARSSSS PQHLNVQQLQ DMYSKMAKTQ SQPILNTKEM 550
    APQSQPQEEL EMGSMPSPVA PLPTRTEGVI DMRSMSSIDS FISCATDFPE 600
    ATRFSHSPLA SLSGKSGGST APEVGWRGAL GASGGRLMET NPIPEASRSG 650
    FFVESPRSSM KTHNPMKLRA LKVNFLEGDP TPLLPALGLY HDPLRNRGGA 700
    AAAVAGLECA SLLDKPVLSP ESSIYTTASA RTPPRSPEKH TAIAFNFEAG 750
    VHQYIDTDTD DEGQLLYSVD SSPPKSLHGS TSPKFSLGAR TEKNHFESSP 800
    LPTSPKFLRP NCVYASEGLP GKGPGAQEKC KLENHTSPDV HMLPGGGAHG 850
    STRDQSI 857
    Length:857
    Mass (Da):95,591
    Last modified:July 27, 2011 - v2
    Checksum:i5FE2D80E58E60710
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti701 – 7011A → R in AAA40112. (PubMed:2002364)Curated
    Sequence conflicti773 – 7731P → L in AAA40112. (PubMed:2002364)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64228 mRNA. Translation: AAA40112.1.
    AL591854, AL591711 Genomic DNA. Translation: CAM13429.1.
    AL591711, AL591854 Genomic DNA. Translation: CAM17304.1.
    CH466551 Genomic DNA. Translation: EDL06500.1.
    BC031776 mRNA. Translation: AAH31776.1.
    BC061501 mRNA. Translation: AAH61501.1.
    CCDSiCCDS17096.1.
    PIRiI56529.
    RefSeqiNP_032446.2. NM_008420.4.
    UniGeneiMm.205341.
    Mm.490804.

    Genome annotation databases

    EnsembliENSMUST00000059826; ENSMUSP00000057981; ENSMUSG00000050556.
    GeneIDi16500.
    KEGGimmu:16500.
    UCSCiuc008nzh.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64228 mRNA. Translation: AAA40112.1 .
    AL591854 , AL591711 Genomic DNA. Translation: CAM13429.1 .
    AL591711 , AL591854 Genomic DNA. Translation: CAM17304.1 .
    CH466551 Genomic DNA. Translation: EDL06500.1 .
    BC031776 mRNA. Translation: AAH31776.1 .
    BC061501 mRNA. Translation: AAH61501.1 .
    CCDSi CCDS17096.1.
    PIRi I56529.
    RefSeqi NP_032446.2. NM_008420.4.
    UniGenei Mm.205341.
    Mm.490804.

    3D structure databases

    ProteinModelPortali Q03717.
    SMRi Q03717. Positions 30-462.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200886. 3 interactions.
    IntActi Q03717. 1 interaction.
    MINTi MINT-8298631.

    Chemistry

    GuidetoPHARMACOLOGYi 546.

    PTM databases

    PhosphoSitei Q03717.

    Proteomic databases

    PaxDbi Q03717.
    PRIDEi Q03717.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000059826 ; ENSMUSP00000057981 ; ENSMUSG00000050556 .
    GeneIDi 16500.
    KEGGi mmu:16500.
    UCSCi uc008nzh.2. mouse.

    Organism-specific databases

    CTDi 3745.
    MGIi MGI:96666. Kcnb1.

    Phylogenomic databases

    eggNOGi COG1226.
    GeneTreei ENSGT00740000114905.
    HOGENOMi HOG000113206.
    HOVERGENi HBG052225.
    InParanoidi Q8K0D1.
    KOi K04885.
    OMAi CATDFPE.
    OrthoDBi EOG7CRTPP.
    TreeFami TF313103.

    Enzyme and pathway databases

    Reactomei REACT_199077. Voltage gated Potassium channels.

    Miscellaneous databases

    ChiTaRSi KCNB1. mouse.
    NextBioi 289823.
    PROi Q03717.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q03717.
    Bgeei Q03717.
    Genevestigatori Q03717.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003973. K_chnl_volt-dep_Kv2.
    IPR004350. K_chnl_volt-dep_Kv2.1.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF03521. Kv2channel. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01514. KV21CHANNEL.
    PR01491. KVCHANNEL.
    PR01495. SHABCHANNEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A mouse brain homolog of the Drosophila Shab K+ channel with conserved delayed-rectifier properties."
      Pak M.D., Covarrubias M., Ratcliffe A., Salkoff L.
      J. Neurosci. 11:869-880(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    6. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiKCNB1_MOUSE
    AccessioniPrimary (citable) accession number: Q03717
    Secondary accession number(s): Q8K0D1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Inhibited by tetraethylammonium chloride (TEA).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3