Q03714 (USA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U1 SNP1-associating protein 1

Gene names

Name:	USA1
Ordered Locus Names:	YML029W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	838 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have a role in pre-mRNA splicing.
Subunit structure	Interacts with SNP1. Ref.3
Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 1890 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from mutant phenotype PubMed 16873066. Source: SGD mRNA splicing, via spliceosome Inferred from physical interaction Ref.3. Source: SGD positive regulation of protein oligomerization Inferred from mutant phenotype PubMed 20005842. Source: SGD
Cellular_component	Hrd1p ubiquitin ligase ERAD-L complex Inferred from direct assay PubMed 16873066. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein complex scaffold Inferred from direct assay PubMed 20005842. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-27760,EBI-27760

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 838	838	U1 SNP1-associating protein 1		PRO_0000114926
Regions
	Topological domain	1 – 536	536	Cytoplasmic Potential
	Transmembrane	537 – 559	23	Helical; Potential
	Topological domain	560 – 563	4	Extracellular Potential
	Transmembrane	564 – 583	20	Helical; Potential
	Topological domain	584 – 838	255	Cytoplasmic Potential
	Domain	259 – 318	60	Ubiquitin-like
Amino acid modifications
	Modified residue	374	1	Phosphoserine Ref.8
	Modified residue	376	1	Phosphoserine Ref.5 Ref.7 Ref.8
	Modified residue	379	1	Phosphoserine Ref.7 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q03714 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 9B93ECA6C5421FD6	FASTA	838	96,653
	10 20 30 40 50 60 MSEYLAQTPC KFTIWSSEID LIRTNLLVNA HPLSTVGRLL QYIHYQIYKQ LRAIYQPEEQ 70 80 90 100 110 120 CTNSEIPHTP LNSINTYFLS YEGRELSATC LLKDITSSSH PDSNHFIRLQ LEKRTSPSGS 130 140 150 160 170 180 AFDLEYDMEG EFNSMNIQFE INTLSSQRIF NSMEPNLPIG TTLARLEKLA LERIKDFEKS 190 200 210 220 230 240 AGNLCGIKED HSVSDLQGFI IKGKQTPMFL NYGSDSDYYK DLNLVDLIGI DFAPAHNSFF 250 260 270 280 290 300 TFLFKMNHEQ NSHIANDEER FVLEFISDAT LSITQMNVKP DTTVKQVKDF ICSVYTHSLN 310 320 330 340 350 360 LRRNDIKLIY KGQLLHENNF AGNSSKISEY IKEPHEVKVH VQINQEYTES GPGFWNEVFN 370 380 390 400 410 420 NPNIFQFMPP DTRSQSPVSF APTQGRSPAA IRGEERGIPY VTESGNDIVP TDELYRKCII 430 440 450 460 470 480 NGDEVVFIPV SELNPQSSYL SVIKGDYGEI KIPISSNDYR INGDNILLSP SAIEQLESAL 490 500 510 520 530 540 NFKIERPRDS TLLHPSGEHV RAADNTSSAN DNNTVENDES AWNRRVVRPL RNSFPLLLVL 550 560 570 580 590 600 IRTFYLIGYN SLVPFFIILE FGSFLPWKYI ILLSLLFIFR TVWNTQEVWN LWRDYLHLNE 610 620 630 640 650 660 IDEVKFSQIK EFINSNSLTL NFYKKCKDTQ SAIDLLMIPN LHEQRLSVYS KYDIEYDTNT 670 680 690 700 710 720 PDVGQLNLLF IKVLSGEIPK DALDELFKEF FELYETTRNM NTLYPQDSLN ELLLMIWKES 730 740 750 760 770 780 QKKDINTLPK YRRWFQTLCS QIAEHNVLDV VLRYIIPDPV NDRVITAVIK NFVLFWVTLL 790 800 810 820 830 PYVKEKLDDI VAQRARDREQ PAPSAQQQEN EDEALIIPDE EEPTATGAQP HLYIPDED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing." Awasthi S., Palmer R., Castro M., Mobarak C.D., Ruby S.W. J. Biol. Chem. 276:31004-31015(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SNP1.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376, MASS SPECTROMETRY. Strain: SUB592.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376 AND SER-379, MASS SPECTROMETRY. Strain: ADR376.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-374; SER-376 AND SER-379, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46659 Genomic DNA. Translation: CAA86626.1. BK006946 Genomic DNA. Translation: DAA09869.1.
PIR	S49750.
RefSeq	NP_013683.1. NM_001182387.1.
3D structure databases
ProteinModelPortal	Q03714.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6423N.
IntAct	Q03714. 28 interactions.
MINT	MINT-673269.
STRING	4932.YML029W.
Proteomic databases
PaxDb	Q03714.
PeptideAtlas	Q03714.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML029W; YML029W; YML029W.
GeneID	854979.
KEGG	sce:YML029W.
Organism-specific databases
CYGD	YML029w.
SGD	S000004491. USA1.
Phylogenomic databases
eggNOG	NOG42991.
HOGENOM	HOG000066037.
KO	K14024.
OrthoDB	EOG4T7CC7.
Gene expression databases
Genevestigator	Q03714.
GermOnline	YML029W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000626. Ubiquitin. IPR019955. Ubiquitin_supergroup. [Graphical view]
Pfam	PF00240. ubiquitin. 1 hit. [Graphical view]
PROSITE	PS50053. UBIQUITIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978094.

Entry information

Entry name

USA1_YEAST

Accession

Primary (citable) accession number: Q03714
Secondary accession number(s): D6VZE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program