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Q03714 (USA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U1 SNP1-associating protein 1
Gene names
Name:USA1
Ordered Locus Names:YML029W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length838 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May have a role in pre-mRNA splicing.

Subunit structure

Interacts with SNP1. Ref.3

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

Present with 1890 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 ubiquitin-like domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself3EBI-27760,EBI-27760
DER1P383073EBI-27760,EBI-5761

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 838838U1 SNP1-associating protein 1
PRO_0000114926

Regions

Topological domain1 – 536536Cytoplasmic Potential
Transmembrane537 – 55923Helical; Potential
Topological domain560 – 5634Extracellular Potential
Transmembrane564 – 58320Helical; Potential
Topological domain584 – 838255Cytoplasmic Potential
Domain259 – 31860Ubiquitin-like

Amino acid modifications

Modified residue3741Phosphoserine Ref.8
Modified residue3761Phosphoserine Ref.6 Ref.8
Modified residue3791Phosphoserine Ref.6 Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q03714 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 9B93ECA6C5421FD6

FASTA83896,653
        10         20         30         40         50         60 
MSEYLAQTPC KFTIWSSEID LIRTNLLVNA HPLSTVGRLL QYIHYQIYKQ LRAIYQPEEQ 

        70         80         90        100        110        120 
CTNSEIPHTP LNSINTYFLS YEGRELSATC LLKDITSSSH PDSNHFIRLQ LEKRTSPSGS 

       130        140        150        160        170        180 
AFDLEYDMEG EFNSMNIQFE INTLSSQRIF NSMEPNLPIG TTLARLEKLA LERIKDFEKS 

       190        200        210        220        230        240 
AGNLCGIKED HSVSDLQGFI IKGKQTPMFL NYGSDSDYYK DLNLVDLIGI DFAPAHNSFF 

       250        260        270        280        290        300 
TFLFKMNHEQ NSHIANDEER FVLEFISDAT LSITQMNVKP DTTVKQVKDF ICSVYTHSLN 

       310        320        330        340        350        360 
LRRNDIKLIY KGQLLHENNF AGNSSKISEY IKEPHEVKVH VQINQEYTES GPGFWNEVFN 

       370        380        390        400        410        420 
NPNIFQFMPP DTRSQSPVSF APTQGRSPAA IRGEERGIPY VTESGNDIVP TDELYRKCII 

       430        440        450        460        470        480 
NGDEVVFIPV SELNPQSSYL SVIKGDYGEI KIPISSNDYR INGDNILLSP SAIEQLESAL 

       490        500        510        520        530        540 
NFKIERPRDS TLLHPSGEHV RAADNTSSAN DNNTVENDES AWNRRVVRPL RNSFPLLLVL 

       550        560        570        580        590        600 
IRTFYLIGYN SLVPFFIILE FGSFLPWKYI ILLSLLFIFR TVWNTQEVWN LWRDYLHLNE 

       610        620        630        640        650        660 
IDEVKFSQIK EFINSNSLTL NFYKKCKDTQ SAIDLLMIPN LHEQRLSVYS KYDIEYDTNT 

       670        680        690        700        710        720 
PDVGQLNLLF IKVLSGEIPK DALDELFKEF FELYETTRNM NTLYPQDSLN ELLLMIWKES 

       730        740        750        760        770        780 
QKKDINTLPK YRRWFQTLCS QIAEHNVLDV VLRYIIPDPV NDRVITAVIK NFVLFWVTLL 

       790        800        810        820        830 
PYVKEKLDDI VAQRARDREQ PAPSAQQQEN EDEALIIPDE EEPTATGAQP HLYIPDED 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing."
Awasthi S., Palmer R., Castro M., Mobarak C.D., Ruby S.W.
J. Biol. Chem. 276:31004-31015(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SNP1.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376 AND SER-379, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-374; SER-376 AND SER-379, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46659 Genomic DNA. Translation: CAA86626.1.
BK006946 Genomic DNA. Translation: DAA09869.1.
PIRS49750.
RefSeqNP_013683.1. NM_001182387.1.

3D structure databases

ProteinModelPortalQ03714.
SMRQ03714. Positions 261-348.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35140. 84 interactions.
DIPDIP-6423N.
IntActQ03714. 25 interactions.
MINTMINT-673269.
STRING4932.YML029W.

Proteomic databases

PaxDbQ03714.
PeptideAtlasQ03714.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML029W; YML029W; YML029W.
GeneID854979.
KEGGsce:YML029W.

Organism-specific databases

CYGDYML029w.
SGDS000004491. USA1.

Phylogenomic databases

eggNOGNOG42991.
HOGENOMHOG000066037.
KOK14024.
OrthoDBEOG7R83BZ.

Enzyme and pathway databases

BioCycYEAST:G3O-32630-MONOMER.

Gene expression databases

GenevestigatorQ03714.

Family and domain databases

InterProIPR000626. Ubiquitin-like.
[Graphical view]
PROSITEPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978094.

Entry information

Entry nameUSA1_YEAST
AccessionPrimary (citable) accession number: Q03714
Secondary accession number(s): D6VZE5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families