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Protein

U1 SNP1-associating protein 1

Gene

USA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scaffold protein of the endoplasmic reticulum-associated degradation (ERAD) (also known as endoplasmic reticulum quality control, ERQC) pathway involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). Has multiple functions in ERAD including recruitment of DER1 to the HRD1 ubiquitin ligase, and regulation of HRD1 activity. Involved in oligomerization of HRD1, which is required for ERAD-L, and in HRD1 autoubiquitination and degradation.5 Publications

GO - Molecular functioni

  • protein complex scaffold Source: SGD

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • mRNA splicing, via spliceosome Source: SGD
  • positive regulation of protein oligomerization Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32630-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U1 SNP1-associating protein 1
Gene namesi
Name:USA1
Ordered Locus Names:YML029W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML029W.
SGDiS000004491. USA1.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 536CytoplasmicSequence analysisAdd BLAST536
Transmembranei537 – 559HelicalSequence analysisAdd BLAST23
Topological domaini560 – 563ExtracellularSequence analysis4
Transmembranei564 – 583HelicalSequence analysisAdd BLAST20
Topological domaini584 – 838CytoplasmicSequence analysisAdd BLAST255

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • Hrd1p ubiquitin ligase ERAD-L complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired degradation of proteins with misfolded lumenal domains such as CPY*, a mutant, misfolded form of carboxypeptidase Y which is a known ERAD-L substrate. Impaired degradation of proteins with misfolded intramembrane domains. Impaired trans-ubiquitination and degradation of the HRD1 ligase. Degradation of proteins with misfolded cytosolic domains is not affected. Interaction of substrate with HRD1 is reduced; in USA1 and YOS9 double mutants this interaction is completely abolished.5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149261 – 838U1 SNP1-associating protein 1Add BLAST838

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei374PhosphoserineCombined sources1
Modified residuei376PhosphoserineCombined sources1
Modified residuei379PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03714.
PRIDEiQ03714.

PTM databases

iPTMnetiQ03714.

Interactioni

Subunit structurei

Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factor YOS9, and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1. Interacts with DER1, HRD1, HRD3, YOS9, UBX2, and CDC48.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-27760,EBI-27760
DER1P383073EBI-27760,EBI-5761

GO - Molecular functioni

  • protein complex scaffold Source: SGD

Protein-protein interaction databases

BioGridi35140. 83 interactors.
DIPiDIP-6423N.
IntActiQ03714. 25 interactors.
MINTiMINT-673269.

Structurei

3D structure databases

ProteinModelPortaliQ03714.
SMRiQ03714.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini259 – 318Ubiquitin-likePROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 240Required for ERAD-L functionAdd BLAST210
Regioni319 – 418Important for HRD1 oligomer formationAdd BLAST100
Regioni345 – 535Interaction with HRD1Add BLAST191
Regioni437 – 490Required for ERAD-L function and HRD1 oligomer formationAdd BLAST54
Regioni584 – 838Interaction with DER1Add BLAST255

Domaini

The ubiquitin-like domain is required for HRD1 trans-ubiquitination and degradation. Reported to be involved in ERAD-M but not ERAD-L function (PubMed:20005842). However, a contradictory report states that it is not required for either ERAD-L or ERAD-M function (PubMed:19940128). Reported to be required for HRD1 oligomer formation (PubMed:19940128). However, a contradictory report does not observe any defects in oligomerization following deletion of the domain (PubMed:21074049).3 Publications

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000066037.
InParanoidiQ03714.
KOiK14024.
OMAiRLLQYIH.
OrthoDBiEOG092C0GYU.

Family and domain databases

InterProiIPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03714-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEYLAQTPC KFTIWSSEID LIRTNLLVNA HPLSTVGRLL QYIHYQIYKQ
60 70 80 90 100
LRAIYQPEEQ CTNSEIPHTP LNSINTYFLS YEGRELSATC LLKDITSSSH
110 120 130 140 150
PDSNHFIRLQ LEKRTSPSGS AFDLEYDMEG EFNSMNIQFE INTLSSQRIF
160 170 180 190 200
NSMEPNLPIG TTLARLEKLA LERIKDFEKS AGNLCGIKED HSVSDLQGFI
210 220 230 240 250
IKGKQTPMFL NYGSDSDYYK DLNLVDLIGI DFAPAHNSFF TFLFKMNHEQ
260 270 280 290 300
NSHIANDEER FVLEFISDAT LSITQMNVKP DTTVKQVKDF ICSVYTHSLN
310 320 330 340 350
LRRNDIKLIY KGQLLHENNF AGNSSKISEY IKEPHEVKVH VQINQEYTES
360 370 380 390 400
GPGFWNEVFN NPNIFQFMPP DTRSQSPVSF APTQGRSPAA IRGEERGIPY
410 420 430 440 450
VTESGNDIVP TDELYRKCII NGDEVVFIPV SELNPQSSYL SVIKGDYGEI
460 470 480 490 500
KIPISSNDYR INGDNILLSP SAIEQLESAL NFKIERPRDS TLLHPSGEHV
510 520 530 540 550
RAADNTSSAN DNNTVENDES AWNRRVVRPL RNSFPLLLVL IRTFYLIGYN
560 570 580 590 600
SLVPFFIILE FGSFLPWKYI ILLSLLFIFR TVWNTQEVWN LWRDYLHLNE
610 620 630 640 650
IDEVKFSQIK EFINSNSLTL NFYKKCKDTQ SAIDLLMIPN LHEQRLSVYS
660 670 680 690 700
KYDIEYDTNT PDVGQLNLLF IKVLSGEIPK DALDELFKEF FELYETTRNM
710 720 730 740 750
NTLYPQDSLN ELLLMIWKES QKKDINTLPK YRRWFQTLCS QIAEHNVLDV
760 770 780 790 800
VLRYIIPDPV NDRVITAVIK NFVLFWVTLL PYVKEKLDDI VAQRARDREQ
810 820 830
PAPSAQQQEN EDEALIIPDE EEPTATGAQP HLYIPDED
Length:838
Mass (Da):96,653
Last modified:November 1, 1997 - v1
Checksum:i9B93ECA6C5421FD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46659 Genomic DNA. Translation: CAA86626.1.
BK006946 Genomic DNA. Translation: DAA09869.1.
PIRiS49750.
RefSeqiNP_013683.1. NM_001182387.1.

Genome annotation databases

EnsemblFungiiYML029W; YML029W; YML029W.
GeneIDi854979.
KEGGisce:YML029W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46659 Genomic DNA. Translation: CAA86626.1.
BK006946 Genomic DNA. Translation: DAA09869.1.
PIRiS49750.
RefSeqiNP_013683.1. NM_001182387.1.

3D structure databases

ProteinModelPortaliQ03714.
SMRiQ03714.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35140. 83 interactors.
DIPiDIP-6423N.
IntActiQ03714. 25 interactors.
MINTiMINT-673269.

PTM databases

iPTMnetiQ03714.

Proteomic databases

MaxQBiQ03714.
PRIDEiQ03714.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML029W; YML029W; YML029W.
GeneIDi854979.
KEGGisce:YML029W.

Organism-specific databases

EuPathDBiFungiDB:YML029W.
SGDiS000004491. USA1.

Phylogenomic databases

HOGENOMiHOG000066037.
InParanoidiQ03714.
KOiK14024.
OMAiRLLQYIH.
OrthoDBiEOG092C0GYU.

Enzyme and pathway databases

BioCyciYEAST:G3O-32630-MONOMER.

Miscellaneous databases

PROiQ03714.

Family and domain databases

InterProiIPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUSA1_YEAST
AccessioniPrimary (citable) accession number: Q03714
Secondary accession number(s): D6VZE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1890 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.