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Q03714

- USA1_YEAST

UniProt

Q03714 - USA1_YEAST

Protein

U1 SNP1-associating protein 1

Gene

USA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Scaffold protein of the endoplasmic reticulum-associated degradation (ERAD) (also known as endoplasmic reticulum quality control, ERQC) pathway involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). Has multiple functions in ERAD including recruitment of DER1 to the HRD1 ubiquitin ligase, and regulation of HRD1 activity. Involved in oligomerization of HRD1, which is required for ERAD-L, and in HRD1 autoubiquitination and degradation.5 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct
    3. protein complex scaffold Source: SGD

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
    2. mRNA splicing, via spliceosome Source: SGD
    3. positive regulation of protein oligomerization Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32630-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U1 SNP1-associating protein 1
    Gene namesi
    Name:USA1
    Ordered Locus Names:YML029W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML029w.
    SGDiS000004491. USA1.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: SGD
    2. Hrd1p ubiquitin ligase ERAD-L complex Source: SGD
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Impaired degradation of proteins with misfolded lumenal domains such as CPY*, a mutant, misfolded form of carboxypeptidase Y which is a known ERAD-L substrate. Impaired degradation of proteins with misfolded intramembrane domains. Impaired trans-ubiquitination and degradation of the HRD1 ligase. Degradation of proteins with misfolded cytosolic domains is not affected. Interaction of substrate with HRD1 is reduced; in USA1 and YOS9 double mutants this interaction is completely abolished.5 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 838838U1 SNP1-associating protein 1PRO_0000114926Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei374 – 3741Phosphoserine1 Publication
    Modified residuei376 – 3761Phosphoserine2 Publications
    Modified residuei379 – 3791Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ03714.
    PaxDbiQ03714.
    PeptideAtlasiQ03714.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03714.

    Interactioni

    Subunit structurei

    Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factor YOS9, and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1. Interacts with DER1, HRD1, HRD3, YOS9, UBX2, and CDC48.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-27760,EBI-27760
    DER1P383073EBI-27760,EBI-5761

    Protein-protein interaction databases

    BioGridi35140. 84 interactions.
    DIPiDIP-6423N.
    IntActiQ03714. 25 interactions.
    MINTiMINT-673269.
    STRINGi4932.YML029W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03714.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 536536CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini560 – 5634ExtracellularSequence Analysis
    Topological domaini584 – 838255CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei537 – 55923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei564 – 58320HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini259 – 31860Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni31 – 240210Required for ERAD-L functionAdd
    BLAST
    Regioni319 – 418100Important for HRD1 oligomer formationAdd
    BLAST
    Regioni345 – 535191Interaction with HRD1Add
    BLAST
    Regioni437 – 49054Required for ERAD-L function and HRD1 oligomer formationAdd
    BLAST
    Regioni584 – 838255Interaction with DER1Add
    BLAST

    Domaini

    The ubiquitin-like domain is required for HRD1 trans-ubiquitination and degradation. Reported to be involved in ERAD-M but not ERAD-L function (PubMed:20005842). However, a contradictory report states that it is not required for either ERAD-L or ERAD-M function (PubMed:19940128). Reported to be required for HRD1 oligomer formation (PubMed:19940128). However, a contradictory report does not observe any defects in oligomerization following deletion of the domain (PubMed:21074049).3 Publications

    Sequence similaritiesi

    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG42991.
    HOGENOMiHOG000066037.
    KOiK14024.
    OMAiNSHIAND.
    OrthoDBiEOG7R83BZ.

    Family and domain databases

    InterProiIPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS50053. UBIQUITIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03714-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEYLAQTPC KFTIWSSEID LIRTNLLVNA HPLSTVGRLL QYIHYQIYKQ    50
    LRAIYQPEEQ CTNSEIPHTP LNSINTYFLS YEGRELSATC LLKDITSSSH 100
    PDSNHFIRLQ LEKRTSPSGS AFDLEYDMEG EFNSMNIQFE INTLSSQRIF 150
    NSMEPNLPIG TTLARLEKLA LERIKDFEKS AGNLCGIKED HSVSDLQGFI 200
    IKGKQTPMFL NYGSDSDYYK DLNLVDLIGI DFAPAHNSFF TFLFKMNHEQ 250
    NSHIANDEER FVLEFISDAT LSITQMNVKP DTTVKQVKDF ICSVYTHSLN 300
    LRRNDIKLIY KGQLLHENNF AGNSSKISEY IKEPHEVKVH VQINQEYTES 350
    GPGFWNEVFN NPNIFQFMPP DTRSQSPVSF APTQGRSPAA IRGEERGIPY 400
    VTESGNDIVP TDELYRKCII NGDEVVFIPV SELNPQSSYL SVIKGDYGEI 450
    KIPISSNDYR INGDNILLSP SAIEQLESAL NFKIERPRDS TLLHPSGEHV 500
    RAADNTSSAN DNNTVENDES AWNRRVVRPL RNSFPLLLVL IRTFYLIGYN 550
    SLVPFFIILE FGSFLPWKYI ILLSLLFIFR TVWNTQEVWN LWRDYLHLNE 600
    IDEVKFSQIK EFINSNSLTL NFYKKCKDTQ SAIDLLMIPN LHEQRLSVYS 650
    KYDIEYDTNT PDVGQLNLLF IKVLSGEIPK DALDELFKEF FELYETTRNM 700
    NTLYPQDSLN ELLLMIWKES QKKDINTLPK YRRWFQTLCS QIAEHNVLDV 750
    VLRYIIPDPV NDRVITAVIK NFVLFWVTLL PYVKEKLDDI VAQRARDREQ 800
    PAPSAQQQEN EDEALIIPDE EEPTATGAQP HLYIPDED 838
    Length:838
    Mass (Da):96,653
    Last modified:November 1, 1997 - v1
    Checksum:i9B93ECA6C5421FD6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46659 Genomic DNA. Translation: CAA86626.1.
    BK006946 Genomic DNA. Translation: DAA09869.1.
    PIRiS49750.
    RefSeqiNP_013683.1. NM_001182387.1.

    Genome annotation databases

    EnsemblFungiiYML029W; YML029W; YML029W.
    GeneIDi854979.
    KEGGisce:YML029W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46659 Genomic DNA. Translation: CAA86626.1 .
    BK006946 Genomic DNA. Translation: DAA09869.1 .
    PIRi S49750.
    RefSeqi NP_013683.1. NM_001182387.1.

    3D structure databases

    ProteinModelPortali Q03714.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35140. 84 interactions.
    DIPi DIP-6423N.
    IntActi Q03714. 25 interactions.
    MINTi MINT-673269.
    STRINGi 4932.YML029W.

    Proteomic databases

    MaxQBi Q03714.
    PaxDbi Q03714.
    PeptideAtlasi Q03714.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML029W ; YML029W ; YML029W .
    GeneIDi 854979.
    KEGGi sce:YML029W.

    Organism-specific databases

    CYGDi YML029w.
    SGDi S000004491. USA1.

    Phylogenomic databases

    eggNOGi NOG42991.
    HOGENOMi HOG000066037.
    KOi K14024.
    OMAi NSHIAND.
    OrthoDBi EOG7R83BZ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32630-MONOMER.

    Miscellaneous databases

    NextBioi 978094.

    Gene expression databases

    Genevestigatori Q03714.

    Family and domain databases

    InterProi IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    4. "Distinct ubiquitin-ligase complexes define convergent pathways for the degradation of ER proteins."
      Carvalho P., Goder V., Rapoport T.A.
      Cell 126:361-373(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE HRD1 COMPLEX, INTERACTION WITH UBX2; HRD1; HRD3; DER1; YOS9 AND CDC48, DISRUPTION PHENOTYPE.
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376 AND SER-379, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: FUNCTION, INTERACTION WITH DER1 AND HRD1, DISRUPTION PHENOTYPE.
    9. "Usa1 protein facilitates substrate ubiquitylation through two separate domains."
      Kim I., Li Y., Muniz P., Rao H.
      PLoS ONE 4:E7604-E7604(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HRD1 AND HRD3, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, CHARACTERIZATION OF UBIQUITIN-LIKE DOMAIN, CHARACTERIZATION OF FUNCTIONAL REGIONS.
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-374; SER-376 AND SER-379, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Retrotranslocation of a misfolded luminal ER protein by the ubiquitin-ligase Hrd1p."
      Carvalho P., Stanley A.M., Rapoport T.A.
      Cell 143:579-591(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HRD1, DISRUPTION PHENOTYPE, CHARACTERIZATION OF UBIQUITIN-LIKE DOMAIN, CHARACTERIZATION OF FUNCTIONAL REGIONS.
    12. "Usa1p is required for optimal function and regulation of the Hrd1p endoplasmic reticulum-associated degradation ubiquitin ligase."
      Carroll S.M., Hampton R.Y.
      J. Biol. Chem. 285:5146-5156(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, CHARACTERIZATION OF UBIQUITIN-LIKE DOMAIN.

    Entry informationi

    Entry nameiUSA1_YEAST
    AccessioniPrimary (citable) accession number: Q03714
    Secondary accession number(s): D6VZE5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1890 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3