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Protein

Colicin-E7 immunity protein

Gene

imm

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.

GO - Molecular functioni

  1. toxic substance binding Source: InterPro

GO - Biological processi

  1. bacteriocin immunity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Bacteriocin immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E7 immunity protein
Alternative name(s):
ImmE7
Microcin-E7 immunity protein
Gene namesi
Name:imm
Synonyms:ceiE7
Encoded oniPlasmid ColE70 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8787Colicin-E7 immunity proteinPRO_0000218708Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
colE7Q471122EBI-1035025,EBI-1035016

Protein-protein interaction databases

DIPiDIP-16989N.
IntActiQ03708. 1 interaction.
MINTiMINT-95483.

Structurei

Secondary structure

1
87
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93Combined sources
Helixi12 – 2514Combined sources
Beta strandi28 – 314Combined sources
Helixi32 – 4514Combined sources
Turni48 – 514Combined sources
Helixi52 – 554Combined sources
Beta strandi59 – 613Combined sources
Helixi65 – 7814Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AYIX-ray2.00A1-87[»]
1CEIX-ray1.80A1-87[»]
1MZ8X-ray2.00A/C1-87[»]
1UJZX-ray2.10A1-87[»]
1UNKX-ray1.80A/B/C/D1-87[»]
1ZNVX-ray2.00A/C1-87[»]
2ERHX-ray2.00A1-87[»]
2JAZX-ray2.03A/C1-87[»]
2JB0X-ray1.91A1-87[»]
2JBGX-ray2.20A/C1-87[»]
2K0DNMR-X2-51[»]
X65-87[»]
4F37X-ray2.57A/B2-87[»]
7CEIX-ray2.30A1-87[»]
ProteinModelPortaliQ03708.
SMRiQ03708. Positions 1-87.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03708.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1200.20. 1 hit.
InterProiIPR023802. Colicin/pyocin_immunity_dom.
IPR000290. Colicin_pyocin.
[Graphical view]
PfamiPF01320. Colicin_Pyocin. 1 hit.
[Graphical view]
PRINTSiPR01299. PYOCIN.
ProDomiPD007225. Colicin_pyocin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47345. SSF47345. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03708-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKNSISDY TEAEFVQLLK EIEKENVAAT DDVLDVLLEH FVKITEHPDG
60 70 80
TDLIYYPSDN RDDSPEGIVK EIKEWRAANG KPGFKQG
Length:87
Mass (Da):9,895
Last modified:November 1, 1997 - v2
Checksum:i2CA7D97057529335
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41K → E in AAA23071 (PubMed:2045785).Curated
Sequence conflicti27 – 271V → E in AAA23071 (PubMed:2045785).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57540 Genomic DNA. Translation: AAA23071.1.
X63620 Genomic DNA. Translation: CAA45165.1.
PIRiS27394.
RefSeqiWP_001560791.1. NC_024962.1.
YP_009060494.1. NC_024962.1.

Genome annotation databases

GeneIDi20467019.
KEGGipg:20467019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57540 Genomic DNA. Translation: AAA23071.1.
X63620 Genomic DNA. Translation: CAA45165.1.
PIRiS27394.
RefSeqiWP_001560791.1. NC_024962.1.
YP_009060494.1. NC_024962.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AYIX-ray2.00A1-87[»]
1CEIX-ray1.80A1-87[»]
1MZ8X-ray2.00A/C1-87[»]
1UJZX-ray2.10A1-87[»]
1UNKX-ray1.80A/B/C/D1-87[»]
1ZNVX-ray2.00A/C1-87[»]
2ERHX-ray2.00A1-87[»]
2JAZX-ray2.03A/C1-87[»]
2JB0X-ray1.91A1-87[»]
2JBGX-ray2.20A/C1-87[»]
2K0DNMR-X2-51[»]
X65-87[»]
4F37X-ray2.57A/B2-87[»]
7CEIX-ray2.30A1-87[»]
ProteinModelPortaliQ03708.
SMRiQ03708. Positions 1-87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-16989N.
IntActiQ03708. 1 interaction.
MINTiMINT-95483.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20467019.
KEGGipg:20467019.

Miscellaneous databases

EvolutionaryTraceiQ03708.

Family and domain databases

Gene3Di1.10.1200.20. 1 hit.
InterProiIPR023802. Colicin/pyocin_immunity_dom.
IPR000290. Colicin_pyocin.
[Graphical view]
PfamiPF01320. Colicin_Pyocin. 1 hit.
[Graphical view]
PRINTSiPR01299. PYOCIN.
ProDomiPD007225. Colicin_pyocin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47345. SSF47345. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the ColE7 plasmid."
    Chak K.-F., Kuo W.S., Lu F.M., James R.
    J. Gen. Microbiol. 137:91-100(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence encoding the immunity and lysis proteins and the carboxyl-terminal peptides of colicins E4 and E7."
    Lau P.C.K., Parsons M.
    Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K317.
  3. "The crystal structure of the immunity protein of colicin E7 suggests a possible colicin-interacting surface."
    Chak K.-F., Safo M.K., Ku W.-Y., Hsieh S.-Y., Yuan H.S.
    Proc. Natl. Acad. Sci. U.S.A. 93:6437-6442(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
  4. "A novel role of ImmE7 in the autoregulatory expression of the ColE7 operon and identification of possible RNase active sites in the crystal structure of dimeric ImmE7."
    Hsieh S.-Y., Ko T.P., Tseng M.Y., Ku W.-Y., Chak K.-F., Yuan H.S.
    EMBO J. 16:1444-1454(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
  5. "The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein."
    Ko T.P., Liao C.C., Ku W.-Y., Chak K.-F., Yuan H.S.
    Structure 7:91-102(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiIMM7_ECOLX
AccessioniPrimary (citable) accession number: Q03708
Secondary accession number(s): Q47113
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 1, 2015
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.