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Q03708 (IMM7_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Colicin-E7 immunity protein
Alternative name(s):
ImmE7
Microcin-E7 immunity protein
Gene names
Name:imm
Synonyms:ceiE7
Encoded onPlasmid ColE7
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length87 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.

Sequence similarities

Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2 family.

Ontologies

Keywords
   Biological processBacteriocin immunity
   Technical term3D-structure
Plasmid
Gene Ontology (GO)
   Biological_processbacteriocin immunity

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiontoxic substance binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

colE7Q471122EBI-1035025,EBI-1035016

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8787Colicin-E7 immunity protein
PRO_0000218708

Experimental info

Sequence conflict41K → E in AAA23071. Ref.1
Sequence conflict271V → E in AAA23071. Ref.1

Secondary structure

............... 87
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q03708 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 2CA7D97057529335

FASTA879,895
        10         20         30         40         50         60 
MELKNSISDY TEAEFVQLLK EIEKENVAAT DDVLDVLLEH FVKITEHPDG TDLIYYPSDN 

        70         80 
RDDSPEGIVK EIKEWRAANG KPGFKQG 

« Hide

References

[1]"Cloning and characterization of the ColE7 plasmid."
Chak K.-F., Kuo W.S., Lu F.M., James R.
J. Gen. Microbiol. 137:91-100(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence encoding the immunity and lysis proteins and the carboxyl-terminal peptides of colicins E4 and E7."
Lau P.C.K., Parsons M.
Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K317.
[3]"The crystal structure of the immunity protein of colicin E7 suggests a possible colicin-interacting surface."
Chak K.-F., Safo M.K., Ku W.-Y., Hsieh S.-Y., Yuan H.S.
Proc. Natl. Acad. Sci. U.S.A. 93:6437-6442(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[4]"A novel role of ImmE7 in the autoregulatory expression of the ColE7 operon and identification of possible RNase active sites in the crystal structure of dimeric ImmE7."
Hsieh S.-Y., Ko T.P., Tseng M.Y., Ku W.-Y., Chak K.-F., Yuan H.S.
EMBO J. 16:1444-1454(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[5]"The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein."
Ko T.P., Liao C.C., Ku W.-Y., Chak K.-F., Yuan H.S.
Structure 7:91-102(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M57540 Genomic DNA. Translation: AAA23071.1.
X63620 Genomic DNA. Translation: CAA45165.1.
PIRS27394.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AYIX-ray2.00A1-87[»]
1CEIX-ray1.80A1-87[»]
1MZ8X-ray2.00A/C1-87[»]
1UJZX-ray2.10A1-87[»]
1UNKX-ray1.80A/B/C/D1-87[»]
1ZNVX-ray2.00A/C1-87[»]
2ERHX-ray2.00A1-87[»]
2JAZX-ray2.03A/C1-87[»]
2JB0X-ray1.91A1-87[»]
2JBGX-ray2.20A/C1-87[»]
2K0DNMR-X2-87[»]
4F37X-ray2.57A/B2-87[»]
7CEIX-ray2.30A1-87[»]
ProteinModelPortalQ03708.
SMRQ03708. Positions 1-87.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-16989N.
IntActQ03708. 1 interaction.
MINTMINT-95483.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1200.20. 1 hit.
InterProIPR023802. Colicin/pyocin_immunity_dom.
IPR000290. Colicin_pyocin.
[Graphical view]
PfamPF01320. Colicin_Pyocin. 1 hit.
[Graphical view]
PRINTSPR01299. PYOCIN.
ProDomPD007225. Colicin_pyocin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF47345. SSF47345. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ03708.

Entry information

Entry nameIMM7_ECOLX
AccessionPrimary (citable) accession number: Q03708
Secondary accession number(s): Q47113
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 16, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references