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Q03708

- IMM7_ECOLX

UniProt

Q03708 - IMM7_ECOLX

Protein

Colicin-E7 immunity protein

Gene

imm

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. toxic substance binding Source: InterPro

    GO - Biological processi

    1. bacteriocin immunity Source: UniProtKB-KW

    Keywords - Biological processi

    Bacteriocin immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Colicin-E7 immunity protein
    Alternative name(s):
    ImmE7
    Microcin-E7 immunity protein
    Gene namesi
    Name:imm
    Synonyms:ceiE7
    Encoded oniPlasmid ColE70 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8787Colicin-E7 immunity proteinPRO_0000218708Add
    BLAST

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    colE7Q471122EBI-1035025,EBI-1035016

    Protein-protein interaction databases

    DIPiDIP-16989N.
    IntActiQ03708. 1 interaction.
    MINTiMINT-95483.

    Structurei

    Secondary structure

    1
    87
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 93
    Helixi12 – 2514
    Beta strandi28 – 314
    Helixi32 – 4514
    Turni48 – 514
    Helixi52 – 554
    Beta strandi59 – 613
    Helixi65 – 7814

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AYIX-ray2.00A1-87[»]
    1CEIX-ray1.80A1-87[»]
    1MZ8X-ray2.00A/C1-87[»]
    1UJZX-ray2.10A1-87[»]
    1UNKX-ray1.80A/B/C/D1-87[»]
    1ZNVX-ray2.00A/C1-87[»]
    2ERHX-ray2.00A1-87[»]
    2JAZX-ray2.03A/C1-87[»]
    2JB0X-ray1.91A1-87[»]
    2JBGX-ray2.20A/C1-87[»]
    2K0DNMR-X2-51[»]
    X65-87[»]
    4F37X-ray2.57A/B2-87[»]
    7CEIX-ray2.30A1-87[»]
    ProteinModelPortaliQ03708.
    SMRiQ03708. Positions 1-87.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ03708.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.1200.20. 1 hit.
    InterProiIPR023802. Colicin/pyocin_immunity_dom.
    IPR000290. Colicin_pyocin.
    [Graphical view]
    PfamiPF01320. Colicin_Pyocin. 1 hit.
    [Graphical view]
    PRINTSiPR01299. PYOCIN.
    ProDomiPD007225. Colicin_pyocin. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF47345. SSF47345. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q03708-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELKNSISDY TEAEFVQLLK EIEKENVAAT DDVLDVLLEH FVKITEHPDG   50
    TDLIYYPSDN RDDSPEGIVK EIKEWRAANG KPGFKQG 87
    Length:87
    Mass (Da):9,895
    Last modified:November 1, 1997 - v2
    Checksum:i2CA7D97057529335
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41K → E in AAA23071. (PubMed:2045785)Curated
    Sequence conflicti27 – 271V → E in AAA23071. (PubMed:2045785)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57540 Genomic DNA. Translation: AAA23071.1.
    X63620 Genomic DNA. Translation: CAA45165.1.
    PIRiS27394.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57540 Genomic DNA. Translation: AAA23071.1 .
    X63620 Genomic DNA. Translation: CAA45165.1 .
    PIRi S27394.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AYI X-ray 2.00 A 1-87 [» ]
    1CEI X-ray 1.80 A 1-87 [» ]
    1MZ8 X-ray 2.00 A/C 1-87 [» ]
    1UJZ X-ray 2.10 A 1-87 [» ]
    1UNK X-ray 1.80 A/B/C/D 1-87 [» ]
    1ZNV X-ray 2.00 A/C 1-87 [» ]
    2ERH X-ray 2.00 A 1-87 [» ]
    2JAZ X-ray 2.03 A/C 1-87 [» ]
    2JB0 X-ray 1.91 A 1-87 [» ]
    2JBG X-ray 2.20 A/C 1-87 [» ]
    2K0D NMR - X 2-51 [» ]
    X 65-87 [» ]
    4F37 X-ray 2.57 A/B 2-87 [» ]
    7CEI X-ray 2.30 A 1-87 [» ]
    ProteinModelPortali Q03708.
    SMRi Q03708. Positions 1-87.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-16989N.
    IntActi Q03708. 1 interaction.
    MINTi MINT-95483.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q03708.

    Family and domain databases

    Gene3Di 1.10.1200.20. 1 hit.
    InterProi IPR023802. Colicin/pyocin_immunity_dom.
    IPR000290. Colicin_pyocin.
    [Graphical view ]
    Pfami PF01320. Colicin_Pyocin. 1 hit.
    [Graphical view ]
    PRINTSi PR01299. PYOCIN.
    ProDomi PD007225. Colicin_pyocin. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF47345. SSF47345. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the ColE7 plasmid."
      Chak K.-F., Kuo W.S., Lu F.M., James R.
      J. Gen. Microbiol. 137:91-100(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Nucleotide sequence encoding the immunity and lysis proteins and the carboxyl-terminal peptides of colicins E4 and E7."
      Lau P.C.K., Parsons M.
      Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K317.
    3. "The crystal structure of the immunity protein of colicin E7 suggests a possible colicin-interacting surface."
      Chak K.-F., Safo M.K., Ku W.-Y., Hsieh S.-Y., Yuan H.S.
      Proc. Natl. Acad. Sci. U.S.A. 93:6437-6442(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
    4. "A novel role of ImmE7 in the autoregulatory expression of the ColE7 operon and identification of possible RNase active sites in the crystal structure of dimeric ImmE7."
      Hsieh S.-Y., Ko T.P., Tseng M.Y., Ku W.-Y., Chak K.-F., Yuan H.S.
      EMBO J. 16:1444-1454(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
    5. "The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein."
      Ko T.P., Liao C.C., Ku W.-Y., Chak K.-F., Yuan H.S.
      Structure 7:91-102(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

    Entry informationi

    Entry nameiIMM7_ECOLX
    AccessioniPrimary (citable) accession number: Q03708
    Secondary accession number(s): Q47113
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3