Q03708 (IMM7_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Colicin-E7 immunity protein

Alternative name(s):
ImmE7
Microcin-E7 immunity protein

Gene names

Name:	imm
Synonyms:	ceiE7

Encoded on

Plasmid ColE7

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.

Sequence similarities

Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2 family.

Ontologies

Keywords
Biological process	Bacteriocin immunity
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological_process	bacteriocin immunity Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	toxin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
colE7	Q47112	2	EBI-1035025,EBI-1035016

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 87

87

Colicin-E7 immunity protein

PRO_0000218708

Experimental info

Sequence conflict

4

1

K → E in AAA23071. Ref.1

Sequence conflict

27

1

V → E in AAA23071. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

87

Helix Strand Turn

Details...

Helix

7 – 9

3

Helix

12 – 25

14

Beta strand

28 – 31

4

Helix

32 – 45

14

Turn

48 – 51

4

Helix

52 – 55

4

Beta strand

58 – 60

3

Helix

65 – 78

14

Sequences

	Sequence		Length	Mass (Da)
	Q03708 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 2CA7D97057529335	FASTA	87	9,895
	10 20 30 40 50 60 MELKNSISDY TEAEFVQLLK EIEKENVAAT DDVLDVLLEH FVKITEHPDG TDLIYYPSDN 70 80 RDDSPEGIVK EIKEWRAANG KPGFKQG

References

[1]	"Cloning and characterization of the ColE7 plasmid." Chak K.-F., Kuo W.S., Lu F.M., James R. J. Gen. Microbiol. 137:91-100(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence encoding the immunity and lysis proteins and the carboxyl-terminal peptides of colicins E4 and E7." Lau P.C.K., Parsons M. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K317.
[3]	"The crystal structure of the immunity protein of colicin E7 suggests a possible colicin-interacting surface." Chak K.-F., Safo M.K., Ku W.-Y., Hsieh S.-Y., Yuan H.S. Proc. Natl. Acad. Sci. U.S.A. 93:6437-6442(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[4]	"A novel role of ImmE7 in the autoregulatory expression of the ColE7 operon and identification of possible RNase active sites in the crystal structure of dimeric ImmE7." Hsieh S.-Y., Ko T.P., Tseng M.Y., Ku W.-Y., Chak K.-F., Yuan H.S. EMBO J. 16:1444-1454(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[5]	"The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein." Ko T.P., Liao C.C., Ku W.-Y., Chak K.-F., Yuan H.S. Structure 7:91-102(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M57540 Genomic DNA. Translation: AAA23071.1.
X63620 Genomic DNA. Translation: CAA45165.1.

PIR

S27394.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AYI	X-ray	2.00	A	1-87	[»]
1CEI	X-ray	1.80	A	1-87	[»]
1MZ8	X-ray	2.00	A/C	1-87	[»]
1UJZ	X-ray	2.10	A	1-87	[»]
1UNK	X-ray	1.80	A/B/C/D	1-87	[»]
1ZNV	X-ray	2.00	A/C	1-87	[»]
2ERH	X-ray	2.00	A	1-87	[»]
2JAZ	X-ray	2.03	A/C	1-87	[»]
2JB0	X-ray	1.91	A	1-87	[»]
2JBG	X-ray	2.20	A/C	1-87	[»]
2K0D	NMR	-	X	2-87	[»]
7CEI	X-ray	2.30	A	1-87	[»]

ProteinModelPortal

Q03708.

SMR

Q03708. Positions 1-87.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-16989N.

IntAct

Q03708. 1 interaction.

MINT

MINT-95483.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.1200.20. 1 hit.

InterPro

IPR023802. Colicin/pyocin_immunity_dom.
IPR000290. Colicin_pyocin.
[Graphical view]

Pfam

PF01320. Colicin_Pyocin. 1 hit.
[Graphical view]

PRINTS

PR01299. PYOCIN.

ProDom

PD007225. Colicin_pyocin. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF47345. Colicin_pyocin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q03708.

Entry information

Entry name

IMM7_ECOLX

Accession

Primary (citable) accession number: Q03708
Secondary accession number(s): Q47113

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program