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Q03708

- IMM7_ECOLX

UniProt

Q03708 - IMM7_ECOLX

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Protein

Colicin-E7 immunity protein

Gene
imm, ceiE7
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. toxic substance binding Source: InterPro

GO - Biological processi

  1. bacteriocin immunity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Bacteriocin immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E7 immunity protein
Alternative name(s):
ImmE7
Microcin-E7 immunity protein
Gene namesi
Name:imm
Synonyms:ceiE7
Encoded oniPlasmid ColE70 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8787Colicin-E7 immunity proteinPRO_0000218708Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
colE7Q471122EBI-1035025,EBI-1035016

Protein-protein interaction databases

DIPiDIP-16989N.
IntActiQ03708. 1 interaction.
MINTiMINT-95483.

Structurei

Secondary structure

1
87
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93
Helixi12 – 2514
Beta strandi28 – 314
Helixi32 – 4514
Turni48 – 514
Helixi52 – 554
Beta strandi59 – 613
Helixi65 – 7814

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AYIX-ray2.00A1-87[»]
1CEIX-ray1.80A1-87[»]
1MZ8X-ray2.00A/C1-87[»]
1UJZX-ray2.10A1-87[»]
1UNKX-ray1.80A/B/C/D1-87[»]
1ZNVX-ray2.00A/C1-87[»]
2ERHX-ray2.00A1-87[»]
2JAZX-ray2.03A/C1-87[»]
2JB0X-ray1.91A1-87[»]
2JBGX-ray2.20A/C1-87[»]
2K0DNMR-X2-51[»]
X65-87[»]
4F37X-ray2.57A/B2-87[»]
7CEIX-ray2.30A1-87[»]
ProteinModelPortaliQ03708.
SMRiQ03708. Positions 1-87.

Miscellaneous databases

EvolutionaryTraceiQ03708.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1200.20. 1 hit.
InterProiIPR023802. Colicin/pyocin_immunity_dom.
IPR000290. Colicin_pyocin.
[Graphical view]
PfamiPF01320. Colicin_Pyocin. 1 hit.
[Graphical view]
PRINTSiPR01299. PYOCIN.
ProDomiPD007225. Colicin_pyocin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47345. SSF47345. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03708-1 [UniParc]FASTAAdd to Basket

« Hide

MELKNSISDY TEAEFVQLLK EIEKENVAAT DDVLDVLLEH FVKITEHPDG   50
TDLIYYPSDN RDDSPEGIVK EIKEWRAANG KPGFKQG 87
Length:87
Mass (Da):9,895
Last modified:November 1, 1997 - v2
Checksum:i2CA7D97057529335
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41K → E in AAA23071. 1 Publication
Sequence conflicti27 – 271V → E in AAA23071. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57540 Genomic DNA. Translation: AAA23071.1.
X63620 Genomic DNA. Translation: CAA45165.1.
PIRiS27394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57540 Genomic DNA. Translation: AAA23071.1 .
X63620 Genomic DNA. Translation: CAA45165.1 .
PIRi S27394.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AYI X-ray 2.00 A 1-87 [» ]
1CEI X-ray 1.80 A 1-87 [» ]
1MZ8 X-ray 2.00 A/C 1-87 [» ]
1UJZ X-ray 2.10 A 1-87 [» ]
1UNK X-ray 1.80 A/B/C/D 1-87 [» ]
1ZNV X-ray 2.00 A/C 1-87 [» ]
2ERH X-ray 2.00 A 1-87 [» ]
2JAZ X-ray 2.03 A/C 1-87 [» ]
2JB0 X-ray 1.91 A 1-87 [» ]
2JBG X-ray 2.20 A/C 1-87 [» ]
2K0D NMR - X 2-51 [» ]
X 65-87 [» ]
4F37 X-ray 2.57 A/B 2-87 [» ]
7CEI X-ray 2.30 A 1-87 [» ]
ProteinModelPortali Q03708.
SMRi Q03708. Positions 1-87.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-16989N.
IntActi Q03708. 1 interaction.
MINTi MINT-95483.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q03708.

Family and domain databases

Gene3Di 1.10.1200.20. 1 hit.
InterProi IPR023802. Colicin/pyocin_immunity_dom.
IPR000290. Colicin_pyocin.
[Graphical view ]
Pfami PF01320. Colicin_Pyocin. 1 hit.
[Graphical view ]
PRINTSi PR01299. PYOCIN.
ProDomi PD007225. Colicin_pyocin. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF47345. SSF47345. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the ColE7 plasmid."
    Chak K.-F., Kuo W.S., Lu F.M., James R.
    J. Gen. Microbiol. 137:91-100(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence encoding the immunity and lysis proteins and the carboxyl-terminal peptides of colicins E4 and E7."
    Lau P.C.K., Parsons M.
    Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K317.
  3. "The crystal structure of the immunity protein of colicin E7 suggests a possible colicin-interacting surface."
    Chak K.-F., Safo M.K., Ku W.-Y., Hsieh S.-Y., Yuan H.S.
    Proc. Natl. Acad. Sci. U.S.A. 93:6437-6442(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
  4. "A novel role of ImmE7 in the autoregulatory expression of the ColE7 operon and identification of possible RNase active sites in the crystal structure of dimeric ImmE7."
    Hsieh S.-Y., Ko T.P., Tseng M.Y., Ku W.-Y., Chak K.-F., Yuan H.S.
    EMBO J. 16:1444-1454(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
  5. "The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein."
    Ko T.P., Liao C.C., Ku W.-Y., Chak K.-F., Yuan H.S.
    Structure 7:91-102(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiIMM7_ECOLX
AccessioniPrimary (citable) accession number: Q03708
Secondary accession number(s): Q47113
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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