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Q03707

- SRC1_YEAST

UniProt

Q03707 - SRC1_YEAST

Protein

Inner nuclear membrane protein SRC1

Gene

SRC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    Plays a role in sister chromatid separation.1 Publication

    GO - Biological processi

    1. maintenance of rDNA Source: SGD
    2. mitotic sister chromatid segregation Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32634-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inner nuclear membrane protein SRC1
    Alternative name(s):
    Helix-extension-helix domain-containing protein 1
    Gene namesi
    Name:SRC1
    Synonyms:HEH1
    Ordered Locus Names:YML034W
    ORF Names:YML033W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML034w1.
    SGDiS000004497. SRC1.

    Subcellular locationi

    Nucleus inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Targeting to the inner nuclear membrane requires the SRP1 and KAP95 karyopherins and the Ran cycle.

    GO - Cellular componenti

    1. integral component of nuclear inner membrane Source: InterPro
    2. nuclear envelope Source: SGD

    Keywords - Cellular componenti

    Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 834834Inner nuclear membrane protein SRC1PRO_0000072188Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei78 – 781Phosphoserine1 Publication
    Modified residuei80 – 801Phosphoserine2 Publications
    Modified residuei85 – 851Phosphoserine2 Publications
    Modified residuei181 – 1811Phosphoserine1 Publication
    Modified residuei203 – 2031Phosphoserine1 Publication
    Modified residuei204 – 2041Phosphoserine2 Publications
    Modified residuei206 – 2061Phosphoserine2 Publications
    Modified residuei301 – 3011Phosphoserine1 Publication
    Modified residuei394 – 3941Phosphothreonine2 Publications
    Modified residuei427 – 4271Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ03707.
    PaxDbiQ03707.
    PeptideAtlasiQ03707.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03707.

    Interactioni

    Protein-protein interaction databases

    BioGridi35136. 163 interactions.
    DIPiDIP-4546N.
    IntActiQ03707. 8 interactions.
    MINTiMINT-552160.
    STRINGi4932.YML034W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03707.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei455 – 47521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei708 – 72821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi117 – 12913Poly-AspAdd
    BLAST

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG292066.
    GeneTreeiENSGT00530000068220.
    HOGENOMiHOG000142043.
    OMAiHENGSEF.
    OrthoDBiEOG7GBG5Q.

    Family and domain databases

    InterProiIPR025856. HeH/LEM_domain.
    IPR018996. Inner-Nucl-membr_MAN1.
    [Graphical view]
    PfamiPF12949. HeH. 1 hit.
    PF09402. MSC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03707-1 [UniParc]FASTAAdd to Basket

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    MNSDLEYLED GFDPNSMKVA TLRRILVENN VDFPSNARKN ALVGLFDEKV    50
    KPQIPQLRKM YLNVRPSDEG IVKMDRPSSS PSIASPRRSR RARREKSASP 100
    MAKQFKKNRI LDDVSNDDDD DDDDDDDNDK KDDPLIVPSG TDTDEVDDEE 150
    DDVITSSSNK SDTNDFQQNS DTRKKRKDPD SDDWSESNSK ENKIDNKHLN 200
    LLSSDSEIEQ DYQKAKKRKT SDLNQEHGNG SAILGKLSVK TPIKNTNRKP 250
    VSMDNFNDSL TSSGTENDPF VPNIRHNPKE LGTANGTGHS TPLSKLKVSA 300
    SFADKLPQKE VPSTILVPEV EQQEPSQSER TPSLFSSEGS GSESEAPLLP 350
    EITTPGPHQP MGNTSNNVVE MIDTDSSNLV SDEDEVLVPT RIETPQLPTE 400
    KDVEKCEARV QELQEEVNEQ LEHENGSEFD VKQGSGKVGN RHKFKRALKF 450
    LSKSLLALFL FCIFIVIPLL FGLWYREQRL LIGYCGHEVP SHRVSGNSFE 500
    FIQKLDNLLQ DYRPKCIPCP PNGICYPYLK LKCKPDYKLA PSRLDFLEII 550
    PAQGKCVKDD KKQQLVSEVV EKSLEFLRAK NAQISCGDGK DDIESGMTED 600
    ALYQIFNEAR APWIRDDEFE DLWIQVIKDL TEEPEILWRQ LSPTDNNIGG 650
    NSNNIIKTND VPRQKRHLPE KFISKTRNFR STSKKYIGMK CRFEREIYQT 700
    YKKFQRPIWL MFLLIVISKV IEIKLKNYYR KKARIEELVT QTMEKLKFQK 750
    IKSMSDPKEN AYLSIVQLRD IFLSDIVDLK YKNQLWSEVV KYLEHNNSNI 800
    KSNLTEIRGE IMKCWEWIGP MELNEPKDSA ENKI 834
    Length:834
    Mass (Da):95,498
    Last modified:September 26, 2001 - v2
    Checksum:i214CA064ECE160F4
    GO

    Sequence cautioni

    The sequence CAA86621.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA86622.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46659 Genomic DNA. Translation: CAA86621.1. Sequence problems.
    Z46659 Genomic DNA. Translation: CAA86622.1. Sequence problems.
    BK006946 Genomic DNA. Translation: DAA09865.1.
    PIRiS49746.
    RefSeqiNP_013679.1. NM_001182391.1.

    Genome annotation databases

    EnsemblFungiiYML034W; YML034W; YML034W.
    GeneIDi854974.
    KEGGisce:YML034W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46659 Genomic DNA. Translation: CAA86621.1 . Sequence problems.
    Z46659 Genomic DNA. Translation: CAA86622.1 . Sequence problems.
    BK006946 Genomic DNA. Translation: DAA09865.1 .
    PIRi S49746.
    RefSeqi NP_013679.1. NM_001182391.1.

    3D structure databases

    ProteinModelPortali Q03707.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35136. 163 interactions.
    DIPi DIP-4546N.
    IntActi Q03707. 8 interactions.
    MINTi MINT-552160.
    STRINGi 4932.YML034W.

    Proteomic databases

    MaxQBi Q03707.
    PaxDbi Q03707.
    PeptideAtlasi Q03707.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML034W ; YML034W ; YML034W .
    GeneIDi 854974.
    KEGGi sce:YML034W.

    Organism-specific databases

    CYGDi YML034w1.
    SGDi S000004497. SRC1.

    Phylogenomic databases

    eggNOGi NOG292066.
    GeneTreei ENSGT00530000068220.
    HOGENOMi HOG000142043.
    OMAi HENGSEF.
    OrthoDBi EOG7GBG5Q.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32634-MONOMER.

    Miscellaneous databases

    NextBioi 978082.

    Gene expression databases

    Genevestigatori Q03707.

    Family and domain databases

    InterProi IPR025856. HeH/LEM_domain.
    IPR018996. Inner-Nucl-membr_MAN1.
    [Graphical view ]
    Pfami PF12949. HeH. 1 hit.
    PF09402. MSC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "SRC1: an intron-containing yeast gene involved in sister chromatid segregation."
      Rodriguez-Navarro S., Igual J.C., Perez-Ortin J.E.
      Yeast 19:43-54(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION OF INTRON.
    4. "Karyopherin-mediated import of integral inner nuclear membrane proteins."
      King M.C., Lusk C.P., Blobel G.
      Nature 442:1003-1007(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-85; SER-204 AND SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301; THR-394 AND SER-427, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78; SER-80; SER-85; SER-181; SER-203; SER-204; SER-206 AND THR-394, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSRC1_YEAST
    AccessioniPrimary (citable) accession number: Q03707
    Secondary accession number(s): D6VZE1, Q03712
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3