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Q03707 (SRC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inner nuclear membrane protein SRC1
Alternative name(s):
Helix-extension-helix domain-containing protein 1
Gene names
Name:SRC1
Synonyms:HEH1
Ordered Locus Names:YML034W
ORF Names:YML033W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in sister chromatid separation. Ref.3

Subcellular location

Nucleus inner membrane; Multi-pass membrane protein. Note: Targeting to the inner nuclear membrane requires the SRP1 and KAP95 karyopherins and the Ran cycle. Ref.4

Sequence caution

The sequence CAA86621.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA86622.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 834834Inner nuclear membrane protein SRC1
PRO_0000072188

Regions

Transmembrane455 – 47521Helical; Potential
Transmembrane708 – 72821Helical; Potential
Compositional bias117 – 12913Poly-Asp

Amino acid modifications

Modified residue781Phosphoserine Ref.8
Modified residue801Phosphoserine Ref.5 Ref.8
Modified residue851Phosphoserine Ref.5 Ref.8
Modified residue1811Phosphoserine Ref.8
Modified residue2031Phosphoserine Ref.8
Modified residue2041Phosphoserine Ref.5 Ref.8
Modified residue2061Phosphoserine Ref.5 Ref.8
Modified residue3011Phosphoserine Ref.7
Modified residue3941Phosphothreonine Ref.7 Ref.8
Modified residue4271Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q03707 [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: 214CA064ECE160F4

FASTA83495,498
        10         20         30         40         50         60 
MNSDLEYLED GFDPNSMKVA TLRRILVENN VDFPSNARKN ALVGLFDEKV KPQIPQLRKM 

        70         80         90        100        110        120 
YLNVRPSDEG IVKMDRPSSS PSIASPRRSR RARREKSASP MAKQFKKNRI LDDVSNDDDD 

       130        140        150        160        170        180 
DDDDDDDNDK KDDPLIVPSG TDTDEVDDEE DDVITSSSNK SDTNDFQQNS DTRKKRKDPD 

       190        200        210        220        230        240 
SDDWSESNSK ENKIDNKHLN LLSSDSEIEQ DYQKAKKRKT SDLNQEHGNG SAILGKLSVK 

       250        260        270        280        290        300 
TPIKNTNRKP VSMDNFNDSL TSSGTENDPF VPNIRHNPKE LGTANGTGHS TPLSKLKVSA 

       310        320        330        340        350        360 
SFADKLPQKE VPSTILVPEV EQQEPSQSER TPSLFSSEGS GSESEAPLLP EITTPGPHQP 

       370        380        390        400        410        420 
MGNTSNNVVE MIDTDSSNLV SDEDEVLVPT RIETPQLPTE KDVEKCEARV QELQEEVNEQ 

       430        440        450        460        470        480 
LEHENGSEFD VKQGSGKVGN RHKFKRALKF LSKSLLALFL FCIFIVIPLL FGLWYREQRL 

       490        500        510        520        530        540 
LIGYCGHEVP SHRVSGNSFE FIQKLDNLLQ DYRPKCIPCP PNGICYPYLK LKCKPDYKLA 

       550        560        570        580        590        600 
PSRLDFLEII PAQGKCVKDD KKQQLVSEVV EKSLEFLRAK NAQISCGDGK DDIESGMTED 

       610        620        630        640        650        660 
ALYQIFNEAR APWIRDDEFE DLWIQVIKDL TEEPEILWRQ LSPTDNNIGG NSNNIIKTND 

       670        680        690        700        710        720 
VPRQKRHLPE KFISKTRNFR STSKKYIGMK CRFEREIYQT YKKFQRPIWL MFLLIVISKV 

       730        740        750        760        770        780 
IEIKLKNYYR KKARIEELVT QTMEKLKFQK IKSMSDPKEN AYLSIVQLRD IFLSDIVDLK 

       790        800        810        820        830 
YKNQLWSEVV KYLEHNNSNI KSNLTEIRGE IMKCWEWIGP MELNEPKDSA ENKI 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"SRC1: an intron-containing yeast gene involved in sister chromatid segregation."
Rodriguez-Navarro S., Igual J.C., Perez-Ortin J.E.
Yeast 19:43-54(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION OF INTRON.
[4]"Karyopherin-mediated import of integral inner nuclear membrane proteins."
King M.C., Lusk C.P., Blobel G.
Nature 442:1003-1007(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-85; SER-204 AND SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[6]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301; THR-394 AND SER-427, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78; SER-80; SER-85; SER-181; SER-203; SER-204; SER-206 AND THR-394, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46659 Genomic DNA. Translation: CAA86621.1. Sequence problems.
Z46659 Genomic DNA. Translation: CAA86622.1. Sequence problems.
BK006946 Genomic DNA. Translation: DAA09865.1.
PIRS49746.
RefSeqNP_013679.1. NM_001182391.1.

3D structure databases

ProteinModelPortalQ03707.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35136. 163 interactions.
DIPDIP-4546N.
IntActQ03707. 8 interactions.
MINTMINT-552160.
STRING4932.YML034W.

Proteomic databases

PaxDbQ03707.
PeptideAtlasQ03707.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML034W; YML034W; YML034W.
GeneID854974.
KEGGsce:YML034W.

Organism-specific databases

CYGDYML034w1.
SGDS000004497. SRC1.

Phylogenomic databases

eggNOGNOG292066.
GeneTreeENSGT00530000068220.
HOGENOMHOG000142043.
OMAHENGSEF.
OrthoDBEOG7GBG5Q.

Enzyme and pathway databases

BioCycYEAST:G3O-32634-MONOMER.

Gene expression databases

GenevestigatorQ03707.

Family and domain databases

InterProIPR025856. HeH/LEM_domain.
IPR018996. Inner-Nucl-membr_MAN1.
[Graphical view]
PfamPF12949. HeH. 1 hit.
PF09402. MSC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978082.

Entry information

Entry nameSRC1_YEAST
AccessionPrimary (citable) accession number: Q03707
Secondary accession number(s): D6VZE1, Q03712
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 26, 2001
Last modified: March 19, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD