Inner nuclear membrane protein SRC1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

Q03707 (SRC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Inner nuclear membrane protein SRC1

Alternative name(s):
Helix-extension-helix domain-containing protein 1

Gene names

Name:	SRC1
Synonyms:	HEH1
Ordered Locus Names:	YML034W
ORF Names:	YML033W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Plays a role in sister chromatid separation. Ref.3

Subcellular location

Nucleus inner membrane; Multi-pass membrane protein. Note: Targeting to the inner nuclear membrane requires the SRP1 and KAP95 karyopherins and the Ran cycle. Ref.4

Sequence caution

The sequence CAA86621.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA86622.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane Nucleus
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maintenance of rDNA Inferred from mutant phenotype PubMed 18997772. Source: SGD mitotic sister chromatid segregation Inferred from genetic interaction Ref.3. Source: SGD
Cellular_component	integral to nuclear inner membrane Inferred from electronic annotation. Source: InterPro nuclear envelope Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 834

834

Inner nuclear membrane protein SRC1

PRO_0000072188

Regions

Transmembrane

455 – 475

Helical; Potential

Transmembrane

708 – 728

Helical; Potential

Compositional bias

117 – 129

Poly-Asp

Amino acid modifications

Modified residue

Phosphoserine Ref.7 Ref.8

Modified residue

Phosphoserine Ref.5 Ref.7 Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.5 Ref.7

Modified residue

203

Phosphoserine Ref.7 Ref.8

Modified residue

204

Phosphoserine Ref.5 Ref.7 Ref.8

Modified residue

206

Phosphoserine Ref.5 Ref.7

Modified residue

221

Phosphoserine Ref.6

Modified residue

241

Phosphothreonine Ref.8

Modified residue

263

Phosphoserine Ref.8

Modified residue

299

Phosphoserine Ref.7

Modified residue

301

Phosphoserine Ref.7 Ref.8

Modified residue

394

Phosphothreonine Ref.8

Modified residue

427

Phosphoserine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

Q03707 [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: 214CA064ECE160F4
Show »

FASTA

834

95,498

        10         20         30         40         50         60 
MNSDLEYLED GFDPNSMKVA TLRRILVENN VDFPSNARKN ALVGLFDEKV KPQIPQLRKM 

        70         80         90        100        110        120 
YLNVRPSDEG IVKMDRPSSS PSIASPRRSR RARREKSASP MAKQFKKNRI LDDVSNDDDD 

       130        140        150        160        170        180 
DDDDDDDNDK KDDPLIVPSG TDTDEVDDEE DDVITSSSNK SDTNDFQQNS DTRKKRKDPD 

       190        200        210        220        230        240 
SDDWSESNSK ENKIDNKHLN LLSSDSEIEQ DYQKAKKRKT SDLNQEHGNG SAILGKLSVK 

       250        260        270        280        290        300 
TPIKNTNRKP VSMDNFNDSL TSSGTENDPF VPNIRHNPKE LGTANGTGHS TPLSKLKVSA 

       310        320        330        340        350        360 
SFADKLPQKE VPSTILVPEV EQQEPSQSER TPSLFSSEGS GSESEAPLLP EITTPGPHQP 

       370        380        390        400        410        420 
MGNTSNNVVE MIDTDSSNLV SDEDEVLVPT RIETPQLPTE KDVEKCEARV QELQEEVNEQ 

       430        440        450        460        470        480 
LEHENGSEFD VKQGSGKVGN RHKFKRALKF LSKSLLALFL FCIFIVIPLL FGLWYREQRL 

       490        500        510        520        530        540 
LIGYCGHEVP SHRVSGNSFE FIQKLDNLLQ DYRPKCIPCP PNGICYPYLK LKCKPDYKLA 

       550        560        570        580        590        600 
PSRLDFLEII PAQGKCVKDD KKQQLVSEVV EKSLEFLRAK NAQISCGDGK DDIESGMTED 

       610        620        630        640        650        660 
ALYQIFNEAR APWIRDDEFE DLWIQVIKDL TEEPEILWRQ LSPTDNNIGG NSNNIIKTND 

       670        680        690        700        710        720 
VPRQKRHLPE KFISKTRNFR STSKKYIGMK CRFEREIYQT YKKFQRPIWL MFLLIVISKV 

       730        740        750        760        770        780 
IEIKLKNYYR KKARIEELVT QTMEKLKFQK IKSMSDPKEN AYLSIVQLRD IFLSDIVDLK 

       790        800        810        820        830 
YKNQLWSEVV KYLEHNNSNI KSNLTEIRGE IMKCWEWIGP MELNEPKDSA ENKI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"SRC1: an intron-containing yeast gene involved in sister chromatid segregation." Rodriguez-Navarro S., Igual J.C., Perez-Ortin J.E. Yeast 19:43-54(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION OF INTRON.
[4]	"Karyopherin-mediated import of integral inner nuclear membrane proteins." King M.C., Lusk C.P., Blobel G. Nature 442:1003-1007(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-85; SER-204 AND SER-206, MASS SPECTROMETRY. Strain: ADR376.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-221, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79; SER-80; SER-85; SER-203; SER-204; SER-206; SER-299 AND SER-301, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79; SER-80; SER-82; SER-203; SER-204; THR-241; SER-263; SER-301; THR-394 AND SER-427, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46659 Genomic DNA. Translation: CAA86621.1. Sequence problems. Z46659 Genomic DNA. Translation: CAA86622.1. Sequence problems. BK006946 Genomic DNA. Translation: DAA09865.1.
PIR	S49746.
RefSeq	NP_013679.1. NM_001182391.1.
3D structure databases
ProteinModelPortal	Q03707.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4546N.
IntAct	Q03707. 8 interactions.
MINT	MINT-552160.
STRING	4932.YML034W.
Proteomic databases
PaxDb	Q03707.
PeptideAtlas	Q03707.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML034W; YML034W; YML034W.
GeneID	854974.
KEGG	sce:YML034W.
Organism-specific databases
CYGD	YML034w1.
SGD	S000004497. SRC1.
Phylogenomic databases
eggNOG	NOG292066.
GeneTree	ENSGT00530000068220.
HOGENOM	HOG000142043.
OrthoDB	EOG43R6VX.
Gene expression databases
Genevestigator	Q03707.
GermOnline	YML034W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR025856. HeH/LEM_domain. IPR018996. Inner-Nucl-membr_MAN1. [Graphical view]
Pfam	PF12949. HeH. 1 hit. PF09402. MSC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978082.

Entry information

Entry name

SRC1_YEAST

Accession

Primary (citable) accession number: Q03707
Secondary accession number(s): D6VZE1, Q03712

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 26, 2001
Last modified:	April 3, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents