Q03705 (CG121_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein CGI121

Gene names

Name:	CGI121
Ordered Locus Names:	YML036W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the EKC/KEOPS complex which promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. Ref.5 Ref.7
Subunit structure	Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1. Ref.5 Ref.7
Subcellular location	Nucleus Probable. Chromosome › telomere Probable.
Sequence similarities	Belongs to the CGI121/TPRKB family.
Sequence caution	The sequence AAS56559.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA86619.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosome Nucleus Telomere
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription from RNA polymerase II promoter Inferred from physical interaction Ref.5. Source: SGD telomere maintenance Inferred from mutant phenotype Ref.7. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	EKC/KEOPS complex Inferred from direct assay Ref.7Ref.5. Source: SGD chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BUD32	P53323	9	EBI-912262,EBI-3809

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Protein CGI121		PRO_0000203259

Sequences

	Sequence		Length	Mass (Da)
	Q03705 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: B0B697F8AB578ED4	FASTA	181	20,662
	10 20 30 40 50 60 MVVSIIPQFP DIKVSLALFE QVKNAKEIRS KMSELSTSFA FIDPRLVCSG EQMYSAIYKT 70 80 90 100 110 120 LIEVKYNKMR TRNLNSECVL CLSPTSNISD AFLKFGIKDD SSQLICLKFH TNTDDVDKEQ 130 140 150 160 170 180 LRTIMTSIVK GQEIEFNDDN LSRFYDEALI RKIYKLSDDF KPQDVNGLSR ALVDAIQLRG V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing." Juneau K., Palm C., Miranda M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-179. Strain: ATCC 201390 / BY4743.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154. Strain: ATCC 204508 / S288c.
[5]	"Yeast homolog of a cancer-testis antigen defines a new transcription complex." Kisseleva-Romanova E., Lopreiato R., Baudin-Baillieu A., Rousselle J.-C., Ilan L., Hofmann K., Namane A., Mann C., Libri D. EMBO J. 25:3576-3585(2006) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE EKC/KEOPS COMPLEX, FUNCTION OF THE EKC/KEOPS COMPLEX.
[6]	"A large-scale full-length cDNA analysis to explore the budding yeast transcriptome." Miura F., Kawaguchi N., Sese J., Toyoda A., Hattori M., Morishita S., Ito T. Proc. Natl. Acad. Sci. U.S.A. 103:17846-17851(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF INTRON.
[7]	"A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator." Downey M., Houlsworth R., Maringele L., Rollie A., Brehme M., Galicia S., Guillard S., Partington M., Zubko M.K., Krogan N.J., Emili A., Greenblatt J.F., Harrington L., Lydall D., Durocher D. Cell 124:1155-1168(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE EKC/KEOPS COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46659 Genomic DNA. Translation: CAA86619.1. Sequence problems. EF123129 mRNA. Translation: ABM97473.1. AY558233 Genomic DNA. Translation: AAS56559.1. Sequence problems. BK006946 Genomic DNA. Translation: DAA09863.1.
PIR	S49743.
RefSeq	NP_013676.2. NM_001182394.1.
3D structure databases
ProteinModelPortal	Q03705.
SMR	Q03705. Positions 9-157.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6420N.
IntAct	Q03705. 7 interactions.
MINT	MINT-2491864.
Proteomic databases
PaxDb	Q03705.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML036W; YML036W; YML036W.
GeneID	854972.
KEGG	sce:YML036W.
Organism-specific databases
SGD	S000004500. CGI121.
Phylogenomic databases
eggNOG	NOG325471.
GeneTree	ENSGT00390000012942.
HOGENOM	HOG000272647.
KO	K15901.
OMA	MECAIAN.
OrthoDB	EOG490BKB.
Gene expression databases
Genevestigator	Q03705.
Family and domain databases
InterPro	IPR013926. CGI121/TPRKB. [Graphical view]
PANTHER	PTHR15840. PTHR15840. 1 hit.
Pfam	PF08617. CGI-121. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978076.

Entry information

Entry name

CG121_YEAST

Accession

Primary (citable) accession number: Q03705
Secondary accession number(s): A2TBM6, D6VZD9, Q6Q5A2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program