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Protein

EKC/KEOPS complex subunit CGI121

Gene

CGI121

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. CGI121 acts as an allosteric effector that regulates the t6A activity of the complex. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. CGI121 is not required for tRNA modification.6 Publications

GO - Biological processi

  • DNA recombination Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • regulation of cell aging Source: SGD
  • telomere maintenance Source: SGD
  • telomere maintenance via recombination Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • tRNA processing Source: UniProtKB-KW

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation, tRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32635-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
EKC/KEOPS complex subunit CGI121
Alternative name(s):
CGI-121 homolog
Gene namesi
Name:CGI121
Ordered Locus Names:YML036W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML036W
SGDiS000004500 CGI121

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002032591 – 181EKC/KEOPS complex subunit CGI121Add BLAST181

Proteomic databases

MaxQBiQ03705
PaxDbiQ03705
PRIDEiQ03705

Interactioni

Subunit structurei

Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUD32P533239EBI-912262,EBI-3809

Protein-protein interaction databases

BioGridi35134, 97 interactors
DIPiDIP-6420N
IntActiQ03705, 7 interactors
MINTiQ03705
STRINGi4932.YML036W

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Beta strandi9 – 21Combined sources13
Helixi25 – 30Combined sources6
Turni31 – 34Combined sources4
Turni36 – 38Combined sources3
Beta strandi40 – 42Combined sources3
Helixi44 – 46Combined sources3
Helixi50 – 66Combined sources17
Beta strandi71 – 73Combined sources3
Helixi74 – 82Combined sources9
Beta strandi83 – 85Combined sources3
Helixi88 – 95Combined sources8
Beta strandi104 – 110Combined sources7
Beta strandi112 – 114Combined sources3
Helixi118 – 128Combined sources11
Beta strandi131 – 134Combined sources4
Helixi138 – 144Combined sources7
Helixi147 – 153Combined sources7
Helixi165 – 178Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WW5X-ray2.00B1-181[»]
4WW7X-ray1.67B1-181[»]
4WW9X-ray1.95B1-181[»]
4WWAX-ray2.95B1-181[»]
4XAHX-ray2.50A/B1-181[»]
ProteinModelPortaliQ03705
SMRiQ03705
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CGI121/TPRKB family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000012942
HOGENOMiHOG000272647
InParanoidiQ03705
KOiK15901
OMAiLQPDPYD
OrthoDBiEOG092C4RLE

Family and domain databases

Gene3Di3.30.2380.10, 1 hit
InterProiView protein in InterPro
IPR013926 CGI121/TPRKB
IPR036504 CGI121/TPRKB_sf
PANTHERiPTHR15840 PTHR15840, 1 hit
PfamiView protein in Pfam
PF08617 CGI-121, 1 hit
SUPFAMiSSF143870 SSF143870, 1 hit

Sequencei

Sequence statusi: Complete.

Q03705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVSIIPQFP DIKVSLALFE QVKNAKEIRS KMSELSTSFA FIDPRLVCSG
60 70 80 90 100
EQMYSAIYKT LIEVKYNKMR TRNLNSECVL CLSPTSNISD AFLKFGIKDD
110 120 130 140 150
SSQLICLKFH TNTDDVDKEQ LRTIMTSIVK GQEIEFNDDN LSRFYDEALI
160 170 180
RKIYKLSDDF KPQDVNGLSR ALVDAIQLRG V
Length:181
Mass (Da):20,662
Last modified:November 1, 1997 - v1
Checksum:iB0B697F8AB578ED4
GO

Sequence cautioni

The sequence AAS56559 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA86619 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46659 Genomic DNA Translation: CAA86619.1 Sequence problems.
EF123129 mRNA Translation: ABM97473.1
AY558233 Genomic DNA Translation: AAS56559.1 Sequence problems.
BK006946 Genomic DNA Translation: DAA09863.1
PIRiS49743
RefSeqiNP_013676.2, NM_001182394.1

Genome annotation databases

EnsemblFungiiYML036W; YML036W; YML036W
GeneIDi854972
KEGGisce:YML036W

Similar proteinsi

Entry informationi

Entry nameiCG121_YEAST
AccessioniPrimary (citable) accession number: Q03705
Secondary accession number(s): A2TBM6, D6VZD9, Q6Q5A2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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