Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q03705

- CG121_YEAST

UniProt

Q03705 - CG121_YEAST

Protein

EKC/KEOPS complex subunit CGI121

Gene

CGI121

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. CGI121 acts as an allosteric effector that regulates the t6A activity of the complex. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. CGI121 is not required for tRNA modification.6 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. DNA recombination Source: SGD
    2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    3. telomere maintenance Source: SGD
    4. telomere maintenance via recombination Source: SGD
    5. transcription, DNA-templated Source: UniProtKB-KW
    6. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation, tRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32635-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EKC/KEOPS complex subunit CGI121
    Alternative name(s):
    CGI-121 homolog
    Gene namesi
    Name:CGI121
    Ordered Locus Names:YML036W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    SGDiS000004500. CGI121.

    Subcellular locationi

    Nucleus Curated. Chromosometelomere Curated

    GO - Cellular componenti

    1. chromosome, telomeric region Source: UniProtKB-SubCell
    2. EKC/KEOPS complex Source: SGD
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chromosome, Nucleus, Telomere

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 181181EKC/KEOPS complex subunit CGI121PRO_0000203259Add
    BLAST

    Proteomic databases

    MaxQBiQ03705.
    PaxDbiQ03705.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03705.

    Interactioni

    Subunit structurei

    Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUD32P533238EBI-912262,EBI-3809

    Protein-protein interaction databases

    BioGridi35134. 17 interactions.
    DIPiDIP-6420N.
    IntActiQ03705. 7 interactions.
    MINTiMINT-2491864.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03705.
    SMRiQ03705. Positions 9-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CGI121/TPRKB family.Curated

    Phylogenomic databases

    eggNOGiNOG325471.
    GeneTreeiENSGT00390000012942.
    HOGENOMiHOG000272647.
    KOiK15901.
    OMAiEREAVNF.
    OrthoDBiEOG7M3JBJ.

    Family and domain databases

    InterProiIPR013926. CGI121/TPRKB.
    [Graphical view]
    PANTHERiPTHR15840. PTHR15840. 1 hit.
    PfamiPF08617. CGI-121. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03705-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVVSIIPQFP DIKVSLALFE QVKNAKEIRS KMSELSTSFA FIDPRLVCSG    50
    EQMYSAIYKT LIEVKYNKMR TRNLNSECVL CLSPTSNISD AFLKFGIKDD 100
    SSQLICLKFH TNTDDVDKEQ LRTIMTSIVK GQEIEFNDDN LSRFYDEALI 150
    RKIYKLSDDF KPQDVNGLSR ALVDAIQLRG V 181
    Length:181
    Mass (Da):20,662
    Last modified:November 1, 1997 - v1
    Checksum:iB0B697F8AB578ED4
    GO

    Sequence cautioni

    The sequence AAS56559.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA86619.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46659 Genomic DNA. Translation: CAA86619.1. Sequence problems.
    EF123129 mRNA. Translation: ABM97473.1.
    AY558233 Genomic DNA. Translation: AAS56559.1. Sequence problems.
    BK006946 Genomic DNA. Translation: DAA09863.1.
    PIRiS49743.
    RefSeqiNP_013676.2. NM_001182394.1.

    Genome annotation databases

    EnsemblFungiiYML036W; YML036W; YML036W.
    GeneIDi854972.
    KEGGisce:YML036W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46659 Genomic DNA. Translation: CAA86619.1 . Sequence problems.
    EF123129 mRNA. Translation: ABM97473.1 .
    AY558233 Genomic DNA. Translation: AAS56559.1 . Sequence problems.
    BK006946 Genomic DNA. Translation: DAA09863.1 .
    PIRi S49743.
    RefSeqi NP_013676.2. NM_001182394.1.

    3D structure databases

    ProteinModelPortali Q03705.
    SMRi Q03705. Positions 9-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35134. 17 interactions.
    DIPi DIP-6420N.
    IntActi Q03705. 7 interactions.
    MINTi MINT-2491864.

    Proteomic databases

    MaxQBi Q03705.
    PaxDbi Q03705.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML036W ; YML036W ; YML036W .
    GeneIDi 854972.
    KEGGi sce:YML036W.

    Organism-specific databases

    SGDi S000004500. CGI121.

    Phylogenomic databases

    eggNOGi NOG325471.
    GeneTreei ENSGT00390000012942.
    HOGENOMi HOG000272647.
    KOi K15901.
    OMAi EREAVNF.
    OrthoDBi EOG7M3JBJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32635-MONOMER.

    Miscellaneous databases

    NextBioi 978076.
    PROi Q03705.

    Gene expression databases

    Genevestigatori Q03705.

    Family and domain databases

    InterProi IPR013926. CGI121/TPRKB.
    [Graphical view ]
    PANTHERi PTHR15840. PTHR15840. 1 hit.
    Pfami PF08617. CGI-121. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
      Juneau K., Palm C., Miranda M., Davis R.W.
      Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-179.
      Strain: ATCC 201390 / BY4743.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154.
      Strain: ATCC 204508 / S288c.
    5. Cited for: IDENTIFICATION IN THE EKC/KEOPS COMPLEX, FUNCTION OF THE EKC/KEOPS COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "A large-scale full-length cDNA analysis to explore the budding yeast transcriptome."
      Miura F., Kawaguchi N., Sese J., Toyoda A., Hattori M., Morishita S., Ito T.
      Proc. Natl. Acad. Sci. U.S.A. 103:17846-17851(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF INTRON.
    7. Cited for: FUNCTION, IDENTIFICATION IN THE EKC/KEOPS COMPLEX.
    8. "The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A."
      Srinivasan M., Mehta P., Yu Y., Prugar E., Koonin E.V., Karzai A.W., Sternglanz R.
      EMBO J. 30:873-881(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN T(6)A37 FORMATION.
    9. Cited for: FUNCTION IN T(6)A37 FORMATION.
    10. "In vitro biosynthesis of a universal t6A tRNA modification in Archaea and Eukarya."
      Perrochia L., Crozat E., Hecker A., Zhang W., Bareille J., Collinet B., van Tilbeurgh H., Forterre P., Basta T.
      Nucleic Acids Res. 41:1953-1964(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN T(6)A37 FORMATION.
    11. "Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system."
      Wan L.C., Mao D.Y., Neculai D., Strecker J., Chiovitti D., Kurinov I., Poda G., Thevakumaran N., Yuan F., Szilard R.K., Lissina E., Nislow C., Caudy A.A., Durocher D., Sicheri F.
      Nucleic Acids Res. 41:6332-6346(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN THE EKC/KEOPS COMPLEX.

    Entry informationi

    Entry nameiCG121_YEAST
    AccessioniPrimary (citable) accession number: Q03705
    Secondary accession number(s): A2TBM6, D6VZD9, Q6Q5A2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3