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Protein

EKC/KEOPS complex subunit CGI121

Gene

CGI121

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. CGI121 acts as an allosteric effector that regulates the t6A activity of the complex. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. CGI121 is not required for tRNA modification.6 Publications

GO - Biological processi

  1. DNA recombination Source: SGD
  2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  3. telomere maintenance Source: SGD
  4. telomere maintenance via recombination Source: SGD
  5. transcription, DNA-templated Source: UniProtKB-KW
  6. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, tRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32635-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
EKC/KEOPS complex subunit CGI121
Alternative name(s):
CGI-121 homolog
Gene namesi
Name:CGI121
Ordered Locus Names:YML036W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

SGDiS000004500. CGI121.

Subcellular locationi

Nucleus Curated. Chromosometelomere Curated

GO - Cellular componenti

  1. chromosome, telomeric region Source: UniProtKB-SubCell
  2. EKC/KEOPS complex Source: SGD
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181EKC/KEOPS complex subunit CGI121PRO_0000203259Add
BLAST

Proteomic databases

MaxQBiQ03705.
PaxDbiQ03705.

Expressioni

Gene expression databases

GenevestigatoriQ03705.

Interactioni

Subunit structurei

Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUD32P533238EBI-912262,EBI-3809

Protein-protein interaction databases

BioGridi35134. 18 interactions.
DIPiDIP-6420N.
IntActiQ03705. 7 interactions.
MINTiMINT-2491864.

Structurei

3D structure databases

ProteinModelPortaliQ03705.
SMRiQ03705. Positions 9-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CGI121/TPRKB family.Curated

Phylogenomic databases

eggNOGiNOG325471.
GeneTreeiENSGT00390000012942.
HOGENOMiHOG000272647.
InParanoidiQ03705.
KOiK15901.
OMAiEREAVNF.
OrthoDBiEOG7M3JBJ.

Family and domain databases

InterProiIPR013926. CGI121/TPRKB.
[Graphical view]
PANTHERiPTHR15840. PTHR15840. 1 hit.
PfamiPF08617. CGI-121. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVSIIPQFP DIKVSLALFE QVKNAKEIRS KMSELSTSFA FIDPRLVCSG
60 70 80 90 100
EQMYSAIYKT LIEVKYNKMR TRNLNSECVL CLSPTSNISD AFLKFGIKDD
110 120 130 140 150
SSQLICLKFH TNTDDVDKEQ LRTIMTSIVK GQEIEFNDDN LSRFYDEALI
160 170 180
RKIYKLSDDF KPQDVNGLSR ALVDAIQLRG V
Length:181
Mass (Da):20,662
Last modified:November 1, 1997 - v1
Checksum:iB0B697F8AB578ED4
GO

Sequence cautioni

The sequence AAS56559.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA86619.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46659 Genomic DNA. Translation: CAA86619.1. Sequence problems.
EF123129 mRNA. Translation: ABM97473.1.
AY558233 Genomic DNA. Translation: AAS56559.1. Sequence problems.
BK006946 Genomic DNA. Translation: DAA09863.1.
PIRiS49743.
RefSeqiNP_013676.2. NM_001182394.1.

Genome annotation databases

EnsemblFungiiYML036W; YML036W; YML036W.
GeneIDi854972.
KEGGisce:YML036W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46659 Genomic DNA. Translation: CAA86619.1. Sequence problems.
EF123129 mRNA. Translation: ABM97473.1.
AY558233 Genomic DNA. Translation: AAS56559.1. Sequence problems.
BK006946 Genomic DNA. Translation: DAA09863.1.
PIRiS49743.
RefSeqiNP_013676.2. NM_001182394.1.

3D structure databases

ProteinModelPortaliQ03705.
SMRiQ03705. Positions 9-157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35134. 18 interactions.
DIPiDIP-6420N.
IntActiQ03705. 7 interactions.
MINTiMINT-2491864.

Proteomic databases

MaxQBiQ03705.
PaxDbiQ03705.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML036W; YML036W; YML036W.
GeneIDi854972.
KEGGisce:YML036W.

Organism-specific databases

SGDiS000004500. CGI121.

Phylogenomic databases

eggNOGiNOG325471.
GeneTreeiENSGT00390000012942.
HOGENOMiHOG000272647.
InParanoidiQ03705.
KOiK15901.
OMAiEREAVNF.
OrthoDBiEOG7M3JBJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-32635-MONOMER.

Miscellaneous databases

NextBioi978076.
PROiQ03705.

Gene expression databases

GenevestigatoriQ03705.

Family and domain databases

InterProiIPR013926. CGI121/TPRKB.
[Graphical view]
PANTHERiPTHR15840. PTHR15840. 1 hit.
PfamiPF08617. CGI-121. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-179.
    Strain: ATCC 201390 / BY4743.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154.
    Strain: ATCC 204508 / S288c.
  5. Cited for: IDENTIFICATION IN THE EKC/KEOPS COMPLEX, FUNCTION OF THE EKC/KEOPS COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "A large-scale full-length cDNA analysis to explore the budding yeast transcriptome."
    Miura F., Kawaguchi N., Sese J., Toyoda A., Hattori M., Morishita S., Ito T.
    Proc. Natl. Acad. Sci. U.S.A. 103:17846-17851(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF INTRON.
  7. Cited for: FUNCTION, IDENTIFICATION IN THE EKC/KEOPS COMPLEX.
  8. "The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A."
    Srinivasan M., Mehta P., Yu Y., Prugar E., Koonin E.V., Karzai A.W., Sternglanz R.
    EMBO J. 30:873-881(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN T(6)A37 FORMATION.
  9. Cited for: FUNCTION IN T(6)A37 FORMATION.
  10. "In vitro biosynthesis of a universal t6A tRNA modification in Archaea and Eukarya."
    Perrochia L., Crozat E., Hecker A., Zhang W., Bareille J., Collinet B., van Tilbeurgh H., Forterre P., Basta T.
    Nucleic Acids Res. 41:1953-1964(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN T(6)A37 FORMATION.
  11. "Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system."
    Wan L.C., Mao D.Y., Neculai D., Strecker J., Chiovitti D., Kurinov I., Poda G., Thevakumaran N., Yuan F., Szilard R.K., Lissina E., Nislow C., Caudy A.A., Durocher D., Sicheri F.
    Nucleic Acids Res. 41:6332-6346(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE EKC/KEOPS COMPLEX.

Entry informationi

Entry nameiCG121_YEAST
AccessioniPrimary (citable) accession number: Q03705
Secondary accession number(s): A2TBM6, D6VZD9, Q6Q5A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.