Q036X3 (HEMH_LACC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	LSEI_1991

Organism

Lactobacillus casei (strain ATCC 334) [Complete proteome] [HAMAP]

Taxonomic identifier

321967 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Ferrochelatase HAMAP MF_00323		PRO_1000019311
Sites
	Metal binding	185	1	Iron By similarity
	Metal binding	267	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q036X3 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 81CD7AE038F07743	FASTA	321	36,274
	10 20 30 40 50 60 MAKGLLIVNL GSPVSPETKD VRRYLREFLS DQNVITMPKA LWQPILRGFI LPFRSWRSAT 70 80 90 100 110 120 FYKHEWTQAG SPLIAYTQVT RDRLRERLPD WDVQMAMNYG GEYPIGETLQ TMAARGDAPI 130 140 150 160 170 180 VVIPLFPEYT QSTTKTILDK VAASGVKTVV IDRFYDHSDY QKILAQQIDD AYEAGAYDTV 190 200 210 220 230 240 ILSYHGIPTA MVRHGDPYQQ ECETTTAGVK QYLKKVPQTK VEMCYQSKFG PVPWLKPYLR 250 260 270 280 290 300 NRLMELAALG KRNVLVATPS FVADCLETLE ENNVQNYQTF RANGGKNFAT VRPMNGCEPF 310 320 CDFLAKLAED KIAAEANHGK A

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 334.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000423 Genomic DNA. Translation: ABJ70749.1.
RefSeq	YP_807191.1. NC_008526.1.
3D structure databases
ProteinModelPortal	Q036X3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q036X3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4420538.
GenomeReviews	Gene locus LSEI_1991 in contig CP000423_GR.
KEGG	lca:LSEI_1991.
PATRIC	22207701. VBILacCas62221_1955.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0276.
HOGENOM	HBG697135.
OMA	IRPAKSA.
PhylomeDB	Q036X3.
ProtClustDB	CLSK821272.
Enzyme and pathway databases
BioCyc	LCAS321967:LSEI_1991-MONOMER.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_LACC3

Accession

Primary (citable) accession number: Q036X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program