Q03691 (ROT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ROT1

Alternative name(s):
Reversal of TOR2 lethality protein 1

Gene names

Name:	ROT1
Ordered Locus Names:	YMR200W
ORF Names:	YM8325.01

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for normal levels of the cell wall 1,6-beta-glucan. Involved in a protein folding machinery chaperoning proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies. Controls actin cytoskeleton polarization to the mother-bud neck and CLB2 protein stability. Ref.3 Ref.4 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein Probable Ref.5 Ref.9 Ref.12.
Post-translational modification	N-glycosylated.
Miscellaneous	Present with 2360 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the ROT1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' protein folding Inferred from direct assay. Source: SGD budding cell apical bud growth Inferred from mutant phenotype Ref.10. Source: SGD cell cycle cytokinesis Inferred from mutant phenotype Ref.10. Source: SGD establishment or maintenance of actin cytoskeleton polarity Inferred from mutant phenotype Ref.10. Source: SGD fungal-type cell wall organization Inferred from mutant phenotype Ref.4. Source: SGD
Cellular component	endoplasmic reticulum membrane Inferred from direct assay Ref.9. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	unfolded protein binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SAC1	P32368	1	EBI-27440,EBI-16210
SWP1	Q02795	1	EBI-27440,EBI-12666

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 256	232	Protein ROT1		PRO_0000203327
Regions
	Topological domain	25 – 235	211	Lumenal Potential
	Transmembrane	236 – 256	21	Helical; Potential
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...)
	Glycosylation	107	1	N-linked (GlcNAc...)
	Glycosylation	139	1	N-linked (GlcNAc...)

Sequences

	Sequence		Length	Mass (Da)
	Q03691 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 58C71DF16296B23C	FASTA	256	28,908
	10 20 30 40 50 60 MWSKKFTLKK LILGGYLFAQ KVYCEDESNS IYGTWSSKSN QVFTGPGFYD PVDELLIEPS 70 80 90 100 110 120 LPGLSYSFTE DGWYEEATYQ VSGNPRNPTC PMASLIYQHG TYNISENGTL VLNPIEVDGR 130 140 150 160 170 180 QLFSDPCNDD GVSTYSRYNQ TETFKEYAVG IDPYHGIYTL QLYQYDGTPM QPLYLAYRPP 190 200 210 220 230 240 MMLPTETLNP TSSATSTDDP SSNKKRSLRS LVRRSLENRH KTNAIKRQNT SFLTSNAIWY 250 ISAGMLGVGS LLFLAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2." Schmidt A., Bickle M., Beck T., Hall M.N. Cell 88:531-542(1997) [PubMed: 9038344] [Abstract] Cited for: FUNCTION.
[4]	"Cell wall integrity modulates RHO1 activity via the exchange factor ROM2." Bickle M., Delley P.-A., Schmidt A., Hall M.N. EMBO J. 17:2235-2245(1998) [PubMed: 9545237] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Rot1p of Saccharomyces cerevisiae is a putative membrane protein required for normal levels of the cell wall 1,6-beta-glucan." Machi K., Azuma M., Igarashi K., Matsumoto T., Fukuda H., Kondo A., Ooshima H. Microbiology 150:3163-3173(2004) [PubMed: 15470097] [Abstract] Cited for: FUNCTION.
[8]	"Causal links between protein folding in the ER and events along the secretory pathway." Takeuchi M., Kimata Y., Kohno K. Autophagy 2:323-324(2006) [PubMed: 16874095] [Abstract] Cited for: FUNCTION.
[9]	"Saccharomyces cerevisiae Rot1p is an ER-localized membrane protein that may function with BiP/Kar2p in protein folding." Takeuchi M., Kimata Y., Hirata A., Oka M., Kohno K. J. Biochem. 139:597-605(2006) [PubMed: 16567426] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]	"Rot1 plays an antagonistic role to Clb2 in actin cytoskeleton dynamics throughout the cell cycle." Juanes M.A., Queralt E., Bano M.C., Igual J.C. J. Cell Sci. 120:2390-2401(2007) [PubMed: 17606994] [Abstract] Cited for: FUNCTION.
[11]	"Dissecting the role of dolichol in cell wall assembly in the yeast mutants impaired in early glycosylation reactions." Orlowski J., Machula K., Janik A., Zdebska E., Palamarczyk G. Yeast 24:239-252(2007) [PubMed: 17397129] [Abstract] Cited for: FUNCTION.
[12]	"Membrane topology and post-translational modification of the Saccharomyces cerevisiae essential protein Rot1." Angeles Juanes M., Carlos Igual J., Carmen Bano M. Yeast 25:93-106(2008) [PubMed: 17914748] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, GLYCOLYSATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48755 Genomic DNA. Translation: CAA88641.1. BK006946 Genomic DNA. Translation: DAA10099.1.
PIR	S59441.
RefSeq	NP_013927.1. NM_001182707.1.
3D structure databases
ProteinModelPortal	Q03691.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2676N.
IntAct	Q03691. 3 interactions.
MINT	MINT-490710.
STRING	Q03691.
Proteomic databases
PeptideAtlas	Q03691.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR200W; YMR200W; YMR200W.
GeneID	855240.
KEGG	sce:YMR200W.
NMPDR	fig|4932.3.peg.4980.
Organism-specific databases
CYGD	YMR200w.
SGD	S000004813. ROT1.
Phylogenomic databases
eggNOG	fuNOG05449.
GeneTree	EFGT00050000006345.
HOGENOM	HBG332450.
OMA	YSFTEDG.
OrthoDB	EOG41NXWF.
Gene expression databases
ArrayExpress	Q03691.
Genevestigator	Q03691.
GermOnline	YMR200W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019623. Chaperone_Rot1_fun. [Graphical view]
Pfam	PF10681. Rot1. 1 hit. [Graphical view]
PIRSF	PIRSF017290. ROT1_prd. 1 hit.
ProtoNet	Search...
Other
NextBio	978792.

Entry information

Entry name

ROT1_YEAST

Accession

Primary (citable) accession number: Q03691
Secondary accession number(s): D6W025

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program