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Protein

Protein ROT1

Gene

ROT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for normal levels of the cell wall 1,6-beta-glucan. Involved in a protein folding machinery chaperoning proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies. Controls actin cytoskeleton polarization to the mother-bud neck and CLB2 protein stability.8 Publications

Miscellaneous

Present with 2360 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • unfolded protein binding Source: SGD

GO - Biological processi

  • 'de novo' protein folding Source: SGD
  • budding cell apical bud growth Source: SGD
  • establishment or maintenance of actin cytoskeleton polarity Source: SGD
  • fungal-type cell wall biogenesis Source: SGD
  • protein folding Source: SGD
  • protein folding in endoplasmic reticulum Source: SGD
  • protein N-linked glycosylation Source: SGD
  • protein O-linked mannosylation Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-32887-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ROT1
Alternative name(s):
Reversal of TOR2 lethality protein 1
Gene namesi
Name:ROT1
Ordered Locus Names:YMR200W
ORF Names:YM8325.01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR200W.
SGDiS000004813. ROT1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 235LumenalSequence analysisAdd BLAST211
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000020332725 – 256Protein ROT1Add BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi103N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi107N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi139N-linked (GlcNAc...) asparagine1 Publication1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Interactioni

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi35378. 295 interactors.
DIPiDIP-2676N.
IntActiQ03691. 14 interactors.
MINTiMINT-490710.
STRINGi4932.YMR200W.

Family & Domainsi

Sequence similaritiesi

Belongs to the ROT1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000246668.
InParanoidiQ03691.
OMAiPPLYLAY.
OrthoDBiEOG092C50WB.

Family and domain databases

InterProiView protein in InterPro
IPR019623. Rot1.
PfamiView protein in Pfam
PF10681. Rot1. 1 hit.
PIRSFiPIRSF017290. ROT1_prd. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWSKKFTLKK LILGGYLFAQ KVYCEDESNS IYGTWSSKSN QVFTGPGFYD
60 70 80 90 100
PVDELLIEPS LPGLSYSFTE DGWYEEATYQ VSGNPRNPTC PMASLIYQHG
110 120 130 140 150
TYNISENGTL VLNPIEVDGR QLFSDPCNDD GVSTYSRYNQ TETFKEYAVG
160 170 180 190 200
IDPYHGIYTL QLYQYDGTPM QPLYLAYRPP MMLPTETLNP TSSATSTDDP
210 220 230 240 250
SSNKKRSLRS LVRRSLENRH KTNAIKRQNT SFLTSNAIWY ISAGMLGVGS

LLFLAF
Length:256
Mass (Da):28,908
Last modified:November 1, 1997 - v1
Checksum:i58C71DF16296B23C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48755 Genomic DNA. Translation: CAA88641.1.
BK006946 Genomic DNA. Translation: DAA10099.1.
PIRiS59441.
RefSeqiNP_013927.1. NM_001182707.1.

Genome annotation databases

EnsemblFungiiYMR200W; YMR200W; YMR200W.
GeneIDi855240.
KEGGisce:YMR200W.

Similar proteinsi

Entry informationi

Entry nameiROT1_YEAST
AccessioniPrimary (citable) accession number: Q03691
Secondary accession number(s): D6W025
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 27, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names