Clustered mitochondria protein 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q03690 (CLU_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Clustered mitochondria protein 1

Alternative name(s):
Translation initiation factor 31
eIF3-associated protein p135

Gene names

Name:	CLU1
Synonyms:	TIF31
Ordered Locus Names:	YMR012W
ORF Names:	YM8270.16

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1277 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in proper cytoplasmic distribution of mitochondria. Ref.4
Subunit structure	May associate with the eukaryotic translation initiation factor 3 (eIF-3) complex. Associates with the 80S ribosome.
Subcellular location	Cytoplasm Ref.5.
Induction	Expression is increased in intermediate compared to fully aerobic conditions. Ref.10
Disruption phenotype	Causes mitochondria to cluster within cells. Ref.4
Miscellaneous	Present with 17000 molecules/cell in log phase SD medium. HAMAP-Rule MF_03013
Sequence similarities	Belongs to the CLU family. Contains 3 TPR repeats.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat TPR repeat
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrion organization Inferred from mutant phenotype Ref.4. Source: SGD translational initiation Inferred from physical interaction Ref.1. Source: SGD
Cellular_component	eukaryotic translation initiation factor 3 complex Inferred from physical interaction Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
URA7	P28274	3	EBI-8989,EBI-20128

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1277

1277

Clustered mitochondria protein 1 HAMAP-Rule MF_03013

PRO_0000123557

Regions

Repeat

704 – 738

TPR 1 HAMAP-Rule MF_03013

Repeat

1020 – 1053

TPR 2 HAMAP-Rule MF_03013

Repeat

1148 – 1181

TPR 3 HAMAP-Rule MF_03013

Amino acid modifications

Modified residue

1247

Phosphoserine Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

Q03690 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 1E643F4EE77ED5B5
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FASTA

1,277

145,166

        10         20         30         40         50         60 
MSEKKEEVKN ATVKVTVKLP KEDNHSHNTK HLKKTQSSKN NDISFEIGKE SKIQTVLDVL 

        70         80         90        100        110        120 
AMIPSSKYLT NVGLKTIEGD SQLSDEMSIK EIVGEKSELK LQLILKPYSA REALKHVITV 

       130        140        150        160        170        180 
RDFIGFAQET SDGLSEFAIS TGSSFSSLPL GPIKERSKQE EKDEKSDPEE KKNTFKDVTD 

       190        200        210        220        230        240 
EEKLKFNEMV HEVFSSFKNS SINKLLTSES NIITPCVRSL SFAPYNPVPP FYRSKGHLFY 

       250        260        270        280        290        300 
LQIVTLEGES FYITAIPSGF YVNKSNSTKF DPSPKENTDE NAHSSLIYYS LFDLIASRSK 

       310        320        330        340        350        360 
KFISHVQAFE KKLSALDSTS YVRPSNTFLH KPWFVSSLPP NNPDYLRLQT AALDTTPERN 

       370        380        390        400        410        420 
FNDEFQAIKD LTTSTLQDRI EMERLFSKVV HEFSVTAASG AMSIFYSDFV AMNPESPTRD 

       430        440        450        460        470        480 
QIFLKDNIFY SYVSDVSGNY EGKGGDEAAI AASNQDLKTI NILNRLHMHE VRYLLTTVVE 

       490        500        510        520        530        540 
FAGRRILAQT PVPGLLATMG NKIVKDANTG EEVTEDFVND INVKYGLDEG LGKIVYDADF 

       550        560        570        580        590        600 
DSVLEKKFVK AFHLKKHKVN GTELAFSSQS KGIVGFDKRR YILDLANTYP LDINFARQNF 

       610        620        630        640        650        660 
DNIEETGNRY PHRQTLLRPE LVEKWWNNKV EKEGVEFEKA YEENLFSYNP DAYQVEGIED 

       670        680        690        700        710        720 
ANVDEMSNYL QKEVIPSVIQ DYLSGNLSTP YNGEHLADTL HKNGINMRYL GKIIELSQKE 

       730        740        750        760        770        780 
LDSQIVHYEQ NLKAVEQDNK EYEDWEKSYL QKIENMIKER QAKINKLVQE GKEVPKELTE 

       790        800        810        820        830        840 
DLKLNDEEIK KPTDGKPVVV AYDELVPLIK ISELEIVSRS LKHVLKDLSK DVPVFLVPSL 

       850        860        870        880        890        900 
VAYVFNMLVG INYNADPKPE PVDEFYPVNK CSFAKLTRSE LLEAVSKQAF LRFRHQLPSN 

       910        920        930        940        950        960 
WIEAYMENPF TLIRSVSYKF GIQLLNKEYF FTREQLESYK QSLDKKIRNK FVEPPTTFSL 

       970        980        990       1000       1010       1020 
SDLTIIPRVK FSEYTSSVSE EFWAQGASMI NEDKQSALTL LAQSITVLED VNNILHPAVA 

      1030       1040       1050       1060       1070       1080 
EKYLSLSAIY NKLALYPEAI AFCRKACTIY ERVSGIDSFE MMRALTNLAI LEFSNESPYN 

      1090       1100       1110       1120       1130       1140 
ATVVYNRLAE ILKVYELPKI HHPAPTSIFN HLEQLALGVQ DTKLAIEVLG QLSSYVVELE 

      1150       1160       1170       1180       1190       1200 
GKDSLAYGYT ESRLGNLFAA LKDFHRALEH ITVTQGIFTK QLGMNHTHSA QSRQWVNGLS 

      1210       1220       1230       1240       1250       1260 
SLIMDLKQKK QLAQDQMSTT GSNSAGHKKT NHRQKKDDVK PELANKSVDE LLTFIEGDSS 

      1270 
NSKSKNKTNN KKKHGKK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 110-kilodalton subunit of translation initiation factor eIF3 and an associated 135-kilodalton protein are encoded by the Saccharomyces cerevisiae TIF32 and TIF31 genes." Vornlocher H.-P., Hanachi P., Ribeiro S., Hershey J.W.B. J. Biol. Chem. 274:16802-16812(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, ASSOCIATION WITH THE EIF-3 COMPLEX. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The S. cerevisiae CLU1 and D. discoideum cluA genes are functional homologues that influence mitochondrial morphology and distribution." Fields S.D., Conrad M.N., Clarke M. J. Cell Sci. 111:1717-1727(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex." Jao D.L., Chen K.Y. J. Cell. Biochem. 97:583-598(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, ASSOCIATION WITH THE 80S RIBOSOME.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1247, MASS SPECTROMETRY.
[10]	"Low oxygen levels as a trigger for enhancement of respiratory metabolism in Saccharomyces cerevisiae." Rintala E., Toivari M., Pitkanen J.P., Wiebe M.G., Ruohonen L., Penttila M. BMC Genomics 10:461-461(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF004911 Genomic DNA. Translation: AAB82415.1. Z48613 Genomic DNA. Translation: CAA88529.1. BK006946 Genomic DNA. Translation: DAA09910.1.
PIR	S53043.
RefSeq	NP_013725.1. NM_001182508.1.
3D structure databases
ProteinModelPortal	Q03690.
SMR	Q03690. Positions 995-1096.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5924N.
IntAct	Q03690. 77 interactions.
MINT	MINT-670532.
STRING	4932.YMR012W.
Proteomic databases
PaxDb	Q03690.
PeptideAtlas	Q03690.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR012W; YMR012W; YMR012W.
GeneID	855025.
KEGG	sce:YMR012W.
Organism-specific databases
CYGD	YMR012w.
SGD	S000004614. CLU1.
Phylogenomic databases
eggNOG	NOG261851.
GeneTree	ENSGT00390000012485.
HOGENOM	HOG000154574.
KO	K03255.
OMA	HEAVNEW.
OrthoDB	EOG4JT3DK.
Gene expression databases
Genevestigator	Q03690.
GermOnline	YMR012W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
HAMAP	MF_03013. CLU.
InterPro	IPR025697. CLU_dom. IPR023231. GSKIP/TIF31_domain. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF13236. CLU. 1 hit. [Graphical view]
SUPFAM	SSF103107. GSKIP/TIF31_domain. 1 hit.
PROSITE	PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	978218.

Entry information

Entry name

CLU_YEAST

Accession

Primary (citable) accession number: Q03690
Secondary accession number(s): D6VZI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents