Q03680 (BLA1_STRCI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase 1
EC=3.5.2.6

Alternative name(s):
Penicillinase

Gene names

Name:

blaL

Organism

Streptomyces cacaoi

Taxonomic identifier

1898 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 325	299	Beta-lactamase 1		PRO_0000017016
Regions
	Region	270 – 272	3	Substrate binding By similarity
Sites
	Active site	93	1	Acyl-ester intermediate Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q03680 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 8E328575B353ABA3	FASTA	325	34,758
	10 20 30 40 50 60 MRIRPTRRLL LGAVAPLALV PLVACGQASG SESGQQPGLG GCGTSAHGSA DAHEKEFRAL 70 80 90 100 110 120 EKKFDAHPGV YAIDTRDGQE ITHRADERFA YGSTFKALQA GAILAQVLRD GREVRRGAEA 130 140 150 160 170 180 DGMDKVVHYG QDAILPNSPV TEKHVADGMS LRELCDAVVA YSDNTAANLL FDQLGGRRGS 190 200 210 220 230 240 TRVLKQLGDH TTSMDRYEQE LGSAVPGDPR DTSTPRAFAE DLRAFAVEDG EKAALAPNDR 250 260 270 280 290 300 EQLNDWMSGS RTGDALIRAG VPKDWKVEDK SGQVKYGTRN DIAVVRPPGR APIVVSVMSH 310 320 GDTQDAEPHD ELVAEAGLVV ADGLK

References

[1]	"Nucleotide sequence of the gene encoding the active-site serine beta-lactamase from Streptomyces cacaoi." Lenzini M.V., Ishihara H., Dusart J., Ogawara H., Joris B., Beeumen J., Frere J.-M., Ghuysen J.-M. FEMS Microbiol. Lett. 49:371-376(1988) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KCC S0352.
[2]	"Beta-lactamase expression in Streptomyces cacaoi." Urabe H., Lenzini M.V., Mukaide M., Dusart J., Nakano M.M., Ghuysen J.-M., Ogawara H. J. Bacteriol. 172:6427-6434(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION. Strain: KCC S0352.
[3]	"The active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G." de Meester F., Joris B., Lenzini M.V., Dehottay P., Erpicium T., Dusart J., Klein D., Ghuysen J.-M., Frere J.-M., van Beeumen J. Biochem. J. 244:427-432(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 89-96, ACTIVE SITE SER-93.
[4]	"Two different beta-lactamase genes are present in Streptomyces cacaoi." Magdalena J., Forsman M., Lenzini M.V., Brans A., Dusart J. FEMS Microbiol. Lett. 78:101-105(1992) [PubMed] [Europe PMC] [Abstract] Cited for: COMPARISON OF BLAU AND BLAL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D90201 Genomic DNA. Translation: BAA14224.1.
PIR	A37148.
3D structure databases
ProteinModelPortal	Q03680.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA1_STRCI

Accession

Primary (citable) accession number: Q03680

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program