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Protein

Beta-lactamase 1

Gene

blaL

Organism
Streptomyces cacaoi
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931Acyl-ester intermediatePROSITE-ProRule annotation1 Publication

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase 1 (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:blaL
OrganismiStreptomyces cacaoi
Taxonomic identifieri1898 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 325299Beta-lactamase 1PRO_0000017016Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ03680.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni270 – 2723Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIRPTRRLL LGAVAPLALV PLVACGQASG SESGQQPGLG GCGTSAHGSA
60 70 80 90 100
DAHEKEFRAL EKKFDAHPGV YAIDTRDGQE ITHRADERFA YGSTFKALQA
110 120 130 140 150
GAILAQVLRD GREVRRGAEA DGMDKVVHYG QDAILPNSPV TEKHVADGMS
160 170 180 190 200
LRELCDAVVA YSDNTAANLL FDQLGGRRGS TRVLKQLGDH TTSMDRYEQE
210 220 230 240 250
LGSAVPGDPR DTSTPRAFAE DLRAFAVEDG EKAALAPNDR EQLNDWMSGS
260 270 280 290 300
RTGDALIRAG VPKDWKVEDK SGQVKYGTRN DIAVVRPPGR APIVVSVMSH
310 320
GDTQDAEPHD ELVAEAGLVV ADGLK
Length:325
Mass (Da):34,758
Last modified:February 1, 1994 - v1
Checksum:i8E328575B353ABA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90201 Genomic DNA. Translation: BAA14224.1.
PIRiA37148.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90201 Genomic DNA. Translation: BAA14224.1.
PIRiA37148.

3D structure databases

ProteinModelPortaliQ03680.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the gene encoding the active-site serine beta-lactamase from Streptomyces cacaoi."
    Lenzini M.V., Ishihara H., Dusart J., Ogawara H., Joris B., Beeumen J., Frere J.-M., Ghuysen J.-M.
    FEMS Microbiol. Lett. 49:371-376(1988)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KCC S0352.
  2. "Beta-lactamase expression in Streptomyces cacaoi."
    Urabe H., Lenzini M.V., Mukaide M., Dusart J., Nakano M.M., Ghuysen J.-M., Ogawara H.
    J. Bacteriol. 172:6427-6434(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
    Strain: KCC S0352.
  3. "The active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G."
    de Meester F., Joris B., Lenzini M.V., Dehottay P., Erpicium T., Dusart J., Klein D., Ghuysen J.-M., Frere J.-M., van Beeumen J.
    Biochem. J. 244:427-432(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 89-96, ACTIVE SITE SER-93.
  4. "Two different beta-lactamase genes are present in Streptomyces cacaoi."
    Magdalena J., Forsman M., Lenzini M.V., Brans A., Dusart J.
    FEMS Microbiol. Lett. 78:101-105(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPARISON OF BLAU AND BLAL.

Entry informationi

Entry nameiBLA1_STRCI
AccessioniPrimary (citable) accession number: Q03680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 1, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.