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Q03677

- MTND_YEAST

UniProt

Q03677 - MTND_YEAST

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Protein

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase

Gene

ADI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).2 PublicationsUniRule annotation

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.1 PublicationUniRule annotation

Cofactori

Binds 1 iron ion per monomer. Can also use other divalent metal cations.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron or nickelUniRule annotation
Metal bindingi87 – 871Iron or nickelUniRule annotation
Metal bindingi91 – 911Iron or nickelUniRule annotation
Metal bindingi132 – 1321Iron or nickelUniRule annotation

GO - Molecular functioni

  1. acireductone dioxygenase (Ni2+-requiring) activity Source: SGD
  2. acireductone dioxygenase [iron(II)-requiring] activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32717-MONOMER.
YEAST:MONOMER3O-186.
ReactomeiREACT_189018. Methionine salvage pathway.
UniPathwayiUPA00904; UER00878.

Names & Taxonomyi

Protein namesi
Recommended name:
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenaseUniRule annotation (EC:1.13.11.54UniRule annotation)
Alternative name(s):
Acireductone dioxygenase (Fe(2+)-requiring)UniRule annotation
Short name:
ARDUniRule annotation
Short name:
Fe-ARDUniRule annotation
Gene namesi
Name:ADI1UniRule annotation
Ordered Locus Names:YMR009W
ORF Names:YM8270.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR009w.
SGDiS000004611. ADI1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi24 – 241E → A: No loss of acireductone dioxygenase activity. 1 Publication
Mutagenesisi45 – 451E → A: No loss of acireductone dioxygenase activity. 1 Publication
Mutagenesisi91 – 911E → A: Loss of acireductone dioxygenase activity. 1 Publication
Mutagenesisi151 – 1511E → A: No loss of acireductone dioxygenase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1791791,2-dihydroxy-3-keto-5-methylthiopentene dioxygenasePRO_0000162946Add
BLAST

Proteomic databases

MaxQBiQ03677.
PaxDbiQ03677.
PeptideAtlasiQ03677.

Expressioni

Inductioni

By heat shock.1 Publication

Gene expression databases

GenevestigatoriQ03677.

Interactioni

Protein-protein interaction databases

BioGridi35178. 40 interactions.
DIPiDIP-877N.
MINTiMINT-565090.
STRINGi4932.YMR009W.

Structurei

3D structure databases

ProteinModelPortaliQ03677.
SMRiQ03677. Positions 1-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acireductone dioxygenase (ARD) family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1791.
GeneTreeiENSGT00390000008195.
HOGENOMiHOG000201071.
InParanoidiQ03677.
KOiK08967.
OMAiTTDESNY.
OrthoDBiEOG7M0P3P.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_03154. Salvage_MtnD_euk.
InterProiIPR004313. Acireductn_dOase_family.
IPR027496. MTCBP-1_eukaryotes.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03677-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVKVYIHDNK VDSDYRAPHN SGTELSLDEL AKLGVIYKYC ANEEEVNEIA
60 70 80 90 100
RQREYKNRDV VNICEGSFKS EAEFNEKLAT FYQEHLHEDE EIRYCLEGAG
110 120 130 140 150
YFDVRDASTP ENWIRCLVES GDLLILPPGI YHRFTLTTSN HIKALRLFKD
160 170
EPKWQAINRS NQADSLPVRK DYIALINQY
Length:179
Mass (Da):20,879
Last modified:November 1, 1997 - v1
Checksum:iE6629DC2E17E2109
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48613 Genomic DNA. Translation: CAA88525.1.
AY558584 Genomic DNA. Translation: AAS56910.1.
BK006946 Genomic DNA. Translation: DAA09907.1.
PIRiS53039.
RefSeqiNP_013722.1. NM_001182505.1.

Genome annotation databases

EnsemblFungiiYMR009W; YMR009W; YMR009W.
GeneIDi855021.
KEGGisce:YMR009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48613 Genomic DNA. Translation: CAA88525.1 .
AY558584 Genomic DNA. Translation: AAS56910.1 .
BK006946 Genomic DNA. Translation: DAA09907.1 .
PIRi S53039.
RefSeqi NP_013722.1. NM_001182505.1.

3D structure databases

ProteinModelPortali Q03677.
SMRi Q03677. Positions 1-176.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35178. 40 interactions.
DIPi DIP-877N.
MINTi MINT-565090.
STRINGi 4932.YMR009W.

Proteomic databases

MaxQBi Q03677.
PaxDbi Q03677.
PeptideAtlasi Q03677.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR009W ; YMR009W ; YMR009W .
GeneIDi 855021.
KEGGi sce:YMR009W.

Organism-specific databases

CYGDi YMR009w.
SGDi S000004611. ADI1.

Phylogenomic databases

eggNOGi COG1791.
GeneTreei ENSGT00390000008195.
HOGENOMi HOG000201071.
InParanoidi Q03677.
KOi K08967.
OMAi TTDESNY.
OrthoDBi EOG7M0P3P.

Enzyme and pathway databases

UniPathwayi UPA00904 ; UER00878 .
BioCyci YEAST:G3O-32717-MONOMER.
YEAST:MONOMER3O-186.
Reactomei REACT_189018. Methionine salvage pathway.

Miscellaneous databases

NextBioi 978207.
PROi Q03677.

Gene expression databases

Genevestigatori Q03677.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
HAMAPi MF_03154. Salvage_MtnD_euk.
InterProi IPR004313. Acireductn_dOase_family.
IPR027496. MTCBP-1_eukaryotes.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR23418. PTHR23418. 1 hit.
Pfami PF03079. ARD. 1 hit.
[Graphical view ]
SUPFAMi SSF51182. SSF51182. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. "Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 (MTCBP-1) acts as an eukaryotic aci-reductone dioxygenase (ARD) in the methionine salvage pathway."
    Hirano W., Gotoh I., Uekita T., Seiki M.
    Genes Cells 10:565-574(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLU-24; GLU-45; GLU-91 AND GLU-151.
  6. "Regulation and surveillance of normal and 3'-extended forms of the yeast aci-reductone dioxygenase mRNA by RNase III cleavage and exonucleolytic degradation."
    Zer C., Chanfreau G.
    J. Biol. Chem. 280:28997-29003(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "A complete inventory of all enzymes in the eukaryotic methionine salvage pathway."
    Pirkov I., Norbeck J., Gustafsson L., Albers E.
    FEBS J. 275:4111-4120(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMTND_YEAST
AccessioniPrimary (citable) accession number: Q03677
Secondary accession number(s): D6VZI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3