Reviewed, UniProtKB/Swiss-Prot Q03677 (MTND_YEAST)

Names and origin

Protein names

Recommended name:
    1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
    EC=1.13.-.-
Alternative name(s):
    Acireductone dioxygenase
      Short name=ARD

Gene names

Name:	ADI1
Ordered Locus Names:	YMR009W
ORF Names:	YM8270.12

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Ref.4
Cofactor	Binds 1 nickel ion per monomer. Can also use other divalent metal cations By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus.
Induction	By heat shock. Ref.5
Sequence similarities	Belongs to the acireductone dioxygenase (ARD) family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cell membrane Membrane Nucleus
Ligand	Metal-binding Nickel
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine salvage Ref.4 Inferred from mutant phenotype. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.3 Inferred from direct assay. Source: SGD nucleus Ref.3 Inferred from direct assay. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Ref.4 Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase		PRO_0000162946
Sites
	Metal binding	85	1	Nickel By similarity
	Metal binding	87	1	Nickel By similarity
	Metal binding	91	1	Nickel By similarity
	Metal binding	132	1	Nickel By similarity
Experimental info
	Mutagenesis	91	1	E → A: Loss of aci-reductone dioxygenase activity. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q03677-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: E6629DC2E17E2109	FASTA	179	20,879
	10 20 30 40 50 60 MVKVYIHDNK VDSDYRAPHN SGTELSLDEL AKLGVIYKYC ANEEEVNEIA RQREYKNRDV 70 80 90 100 110 120 VNICEGSFKS EAEFNEKLAT FYQEHLHEDE EIRYCLEGAG YFDVRDASTP ENWIRCLVES 130 140 150 160 170 GDLLILPPGI YHRFTLTTSN HIKALRLFKD EPKWQAINRS NQADSLPVRK DYIALINQY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 (MTCBP-1) acts as an eukaryotic aci-reductone dioxygenase (ARD) in the methionine salvage pathway." Hirano W., Gotoh I., Uekita T., Seiki M. Genes Cells 10:565-574(2005) [PubMed: 15938715] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-91.
[5]	"Regulation and surveillance of normal and 3'-extended forms of the yeast aci-reductone dioxygenase mRNA by RNase III cleavage and exonucleolytic degradation." Zer C., Chanfreau G. J. Biol. Chem. 280:28997-29003(2005) [PubMed: 15967792] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z48613 Genomic DNA. Translation: CAA88525.1. AY558584 Genomic DNA. Translation: AAS56910.1.
PIR	S53039.
RefSeq	NP_013722.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:877N.
IntAct	Q03677. 3 interactions.
STRING	Q03677.
Proteomic databases
PeptideAtlas	Q03677.
Genome annotation databases
Ensembl	YMR009W; YMR009W; YMR009W; Saccharomyces cerevisiae. [Genome view]
GeneID	855021.
KEGG	sce:YMR009W.
NMPDR	fig|4932.3.peg.4766.
Organism-specific databases
CYGD	YMR009w.
SGD	S000004611. ADI1.
Phylogenomic databases
HOGENOM	Q03677.
OMA	PARKEYV
OrthoDB	EOG95X9C4
Gene expression databases
ArrayExpress	Q03677.
Genevestigator	Q03677.
GermOnline	YMR009W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004313. Acireductn_dOase. IPR011051. Cupin_RmlC_type. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
PANTHER	PTHR23418. Acireductn_dOase. 1 hit.
Pfam	PF03079. ARD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978207.

Entry information

Entry name

MTND_YEAST

Accession

Primary (citable) accession number: Q03677

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)