Reviewed,
UniProtKB/Swiss-Prot Q03677 (MTND_YEAST)
Last modified
November 24, 2009.
Version 73.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase EC=1.13.-.- Alternative name(s): Acireductone dioxygenase Short name=ARD | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 179 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Ref.4 |
| Cofactor | Binds 1 nickel ion per monomer. Can also use other divalent metal cations By similarity. |
| Pathway | |
| Subcellular location | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. |
| Induction | By heat shock. Ref.5 |
| Sequence similarities | Belongs to the acireductone dioxygenase (ARD) family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Methionine biosynthesis |
| Cellular component | Cell membrane Membrane Nucleus |
| Ligand | Metal-binding Nickel |
| Molecular function | Dioxygenase Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine salvage Ref.4Inferred from mutant phenotype. Source: SGD oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Ref.3 Inferred from direct assay. Source: SGD nucleus Ref.3Inferred from direct assay. Source: SGD plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Ref.4Inferred from genetic interaction. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 179 | 179 | 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase | PRO_0000162946 | |||||
Sites | |||||||||
| Metal binding | 85 | 1 | Nickel By similarity | ||||||
| Metal binding | 87 | 1 | Nickel By similarity | ||||||
| Metal binding | 91 | 1 | Nickel By similarity | ||||||
| Metal binding | 132 | 1 | Nickel By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 91 | 1 | E → A: Loss of aci-reductone dioxygenase activity. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G.Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [2] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [4] | "Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 (MTCBP-1) acts as an eukaryotic aci-reductone dioxygenase (ARD) in the methionine salvage pathway." Hirano W., Gotoh I., Uekita T., Seiki M. Genes Cells 10:565-574(2005) [PubMed: 15938715] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-91. |
| [5] | "Regulation and surveillance of normal and 3'-extended forms of the yeast aci-reductone dioxygenase mRNA by RNase III cleavage and exonucleolytic degradation." Zer C., Chanfreau G. J. Biol. Chem. 280:28997-29003(2005) [PubMed: 15967792] [Abstract] Cited for: INDUCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Z48613 Genomic DNA. Translation: CAA88525.1. AY558584 Genomic DNA. Translation: AAS56910.1. | |
| PIR | S53039. |
| RefSeq | NP_013722.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:877N. |
| IntAct | Q03677. 3 interactions. |
| STRING | Q03677. |
Proteomic databases | |
| PeptideAtlas | Q03677. |
Genome annotation databases | |
| Ensembl | YMR009W; YMR009W; YMR009W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 855021. |
| KEGG | sce:YMR009W. |
| NMPDR | fig|4932.3.peg.4766. |
Organism-specific databases | |
| CYGD | YMR009w. |
| SGD | S000004611. ADI1. |
Phylogenomic databases | |
| HOGENOM | Q03677. |
| OMA | PARKEYV |
| OrthoDB | EOG95X9C4 |
Gene expression databases | |
| ArrayExpress | Q03677. |
| Genevestigator | Q03677. |
| GermOnline | YMR009W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR004313. Acireductn_dOase. IPR011051. Cupin_RmlC_type. IPR014710. RmlC-like_jellyroll. [Graphical view] |
| Gene3D | G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit. |
| PANTHER | PTHR23418. Acireductn_dOase. 1 hit. |
| Pfam | PF03079. ARD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 978207. |
Entry information
| Entry name | MTND_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q03677 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIII Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names |

Clusters with


