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Protein

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase

Gene

ADI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniRule annotation2 Publications

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.UniRule annotation1 Publication

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per monomer. Can also use other divalent metal cations.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron or nickelUniRule annotation
Metal bindingi87 – 871Iron or nickelUniRule annotation
Metal bindingi91 – 911Iron or nickelUniRule annotation
Metal bindingi132 – 1321Iron or nickelUniRule annotation

GO - Molecular functioni

  1. acireductone dioxygenase (Ni2+-requiring) activity Source: SGD
  2. acireductone dioxygenase [iron(II)-requiring] activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32717-MONOMER.
YEAST:MONOMER3O-186.
ReactomeiREACT_350157. Methionine salvage pathway.
UniPathwayiUPA00904; UER00878.

Names & Taxonomyi

Protein namesi
Recommended name:
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenaseUniRule annotation (EC:1.13.11.54UniRule annotation)
Alternative name(s):
Acireductone dioxygenase (Fe(2+)-requiring)UniRule annotation
Short name:
ARDUniRule annotation
Short name:
Fe-ARDUniRule annotation
Gene namesi
Name:ADI1UniRule annotation
Ordered Locus Names:YMR009W
ORF Names:YM8270.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR009w.
SGDiS000004611. ADI1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi24 – 241E → A: No loss of acireductone dioxygenase activity. 1 Publication
Mutagenesisi45 – 451E → A: No loss of acireductone dioxygenase activity. 1 Publication
Mutagenesisi91 – 911E → A: Loss of acireductone dioxygenase activity. 1 Publication
Mutagenesisi151 – 1511E → A: No loss of acireductone dioxygenase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1791791,2-dihydroxy-3-keto-5-methylthiopentene dioxygenasePRO_0000162946Add
BLAST

Proteomic databases

MaxQBiQ03677.
PaxDbiQ03677.
PeptideAtlasiQ03677.

Expressioni

Inductioni

By heat shock.1 Publication

Gene expression databases

GenevestigatoriQ03677.

Interactioni

Protein-protein interaction databases

BioGridi35178. 39 interactions.
DIPiDIP-877N.
MINTiMINT-565090.
STRINGi4932.YMR009W.

Structurei

3D structure databases

SMRiQ03677. Positions 1-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acireductone dioxygenase (ARD) family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1791.
GeneTreeiENSGT00390000008195.
HOGENOMiHOG000201071.
InParanoidiQ03677.
KOiK08967.
OMAiNNDLDAY.
OrthoDBiEOG7M0P3P.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_03154. Salvage_MtnD_euk.
InterProiIPR004313. Acireductn_dOase_family.
IPR027496. MTCBP-1_eukaryotes.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKVYIHDNK VDSDYRAPHN SGTELSLDEL AKLGVIYKYC ANEEEVNEIA
60 70 80 90 100
RQREYKNRDV VNICEGSFKS EAEFNEKLAT FYQEHLHEDE EIRYCLEGAG
110 120 130 140 150
YFDVRDASTP ENWIRCLVES GDLLILPPGI YHRFTLTTSN HIKALRLFKD
160 170
EPKWQAINRS NQADSLPVRK DYIALINQY
Length:179
Mass (Da):20,879
Last modified:November 1, 1997 - v1
Checksum:iE6629DC2E17E2109
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48613 Genomic DNA. Translation: CAA88525.1.
AY558584 Genomic DNA. Translation: AAS56910.1.
BK006946 Genomic DNA. Translation: DAA09907.1.
PIRiS53039.
RefSeqiNP_013722.1. NM_001182505.1.

Genome annotation databases

EnsemblFungiiYMR009W; YMR009W; YMR009W.
GeneIDi855021.
KEGGisce:YMR009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48613 Genomic DNA. Translation: CAA88525.1.
AY558584 Genomic DNA. Translation: AAS56910.1.
BK006946 Genomic DNA. Translation: DAA09907.1.
PIRiS53039.
RefSeqiNP_013722.1. NM_001182505.1.

3D structure databases

SMRiQ03677. Positions 1-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35178. 39 interactions.
DIPiDIP-877N.
MINTiMINT-565090.
STRINGi4932.YMR009W.

Proteomic databases

MaxQBiQ03677.
PaxDbiQ03677.
PeptideAtlasiQ03677.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR009W; YMR009W; YMR009W.
GeneIDi855021.
KEGGisce:YMR009W.

Organism-specific databases

CYGDiYMR009w.
SGDiS000004611. ADI1.

Phylogenomic databases

eggNOGiCOG1791.
GeneTreeiENSGT00390000008195.
HOGENOMiHOG000201071.
InParanoidiQ03677.
KOiK08967.
OMAiNNDLDAY.
OrthoDBiEOG7M0P3P.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00878.
BioCyciYEAST:G3O-32717-MONOMER.
YEAST:MONOMER3O-186.
ReactomeiREACT_350157. Methionine salvage pathway.

Miscellaneous databases

NextBioi978207.
PROiQ03677.

Gene expression databases

GenevestigatoriQ03677.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_03154. Salvage_MtnD_euk.
InterProiIPR004313. Acireductn_dOase_family.
IPR027496. MTCBP-1_eukaryotes.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. "Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 (MTCBP-1) acts as an eukaryotic aci-reductone dioxygenase (ARD) in the methionine salvage pathway."
    Hirano W., Gotoh I., Uekita T., Seiki M.
    Genes Cells 10:565-574(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLU-24; GLU-45; GLU-91 AND GLU-151.
  6. "Regulation and surveillance of normal and 3'-extended forms of the yeast aci-reductone dioxygenase mRNA by RNase III cleavage and exonucleolytic degradation."
    Zer C., Chanfreau G.
    J. Biol. Chem. 280:28997-29003(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "A complete inventory of all enzymes in the eukaryotic methionine salvage pathway."
    Pirkov I., Norbeck J., Gustafsson L., Albers E.
    FEBS J. 275:4111-4120(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMTND_YEAST
AccessioniPrimary (citable) accession number: Q03677
Secondary accession number(s): D6VZI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 1, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.