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Q03677

- MTND_YEAST

UniProt

Q03677 - MTND_YEAST

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Protein
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Gene
ADI1, YMR009W, YM8270.12
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).2 Publications

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.1 Publication

Cofactori

Binds 1 iron ion per monomer. Can also use other divalent metal cations By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron or nickel By similarity
Metal bindingi87 – 871Iron or nickel By similarity
Metal bindingi91 – 911Iron or nickel By similarity
Metal bindingi132 – 1321Iron or nickel By similarity

GO - Molecular functioni

  1. acireductone dioxygenase (Ni2+-requiring) activity Source: SGD
  2. acireductone dioxygenase [iron(II)-requiring] activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. L-methionine salvage from methylthioadenosine Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32717-MONOMER.
YEAST:MONOMER3O-186.
ReactomeiREACT_189018. Methionine salvage pathway.
UniPathwayiUPA00904; UER00878.

Names & Taxonomyi

Protein namesi
Recommended name:
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (EC:1.13.11.54)
Alternative name(s):
Acireductone dioxygenase (Fe(2+)-requiring)
Short name:
ARD
Short name:
Fe-ARD
Gene namesi
Name:ADI1
Ordered Locus Names:YMR009W
ORF Names:YM8270.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR009w.
SGDiS000004611. ADI1.

Subcellular locationi

Cytoplasm. Nucleus 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi24 – 241E → A: No loss of acireductone dioxygenase activity. 1 Publication
Mutagenesisi45 – 451E → A: No loss of acireductone dioxygenase activity. 1 Publication
Mutagenesisi91 – 911E → A: Loss of acireductone dioxygenase activity. 1 Publication
Mutagenesisi151 – 1511E → A: No loss of acireductone dioxygenase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1791791,2-dihydroxy-3-keto-5-methylthiopentene dioxygenaseUniRule annotation
PRO_0000162946Add
BLAST

Proteomic databases

MaxQBiQ03677.
PaxDbiQ03677.
PeptideAtlasiQ03677.

Expressioni

Inductioni

By heat shock.1 Publication

Gene expression databases

GenevestigatoriQ03677.

Interactioni

Protein-protein interaction databases

BioGridi35178. 40 interactions.
DIPiDIP-877N.
MINTiMINT-565090.
STRINGi4932.YMR009W.

Structurei

3D structure databases

ProteinModelPortaliQ03677.
SMRiQ03677. Positions 1-176.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1791.
GeneTreeiENSGT00390000008195.
HOGENOMiHOG000201071.
KOiK08967.
OMAiTTDESNY.
OrthoDBiEOG7M0P3P.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_03154. Salvage_MtnD_euk.
InterProiIPR004313. Acireductn_dOase_family.
IPR027496. MTCBP-1_eukaryotes.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03677-1 [UniParc]FASTAAdd to Basket

« Hide

MVKVYIHDNK VDSDYRAPHN SGTELSLDEL AKLGVIYKYC ANEEEVNEIA    50
RQREYKNRDV VNICEGSFKS EAEFNEKLAT FYQEHLHEDE EIRYCLEGAG 100
YFDVRDASTP ENWIRCLVES GDLLILPPGI YHRFTLTTSN HIKALRLFKD 150
EPKWQAINRS NQADSLPVRK DYIALINQY 179
Length:179
Mass (Da):20,879
Last modified:November 1, 1997 - v1
Checksum:iE6629DC2E17E2109
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48613 Genomic DNA. Translation: CAA88525.1.
AY558584 Genomic DNA. Translation: AAS56910.1.
BK006946 Genomic DNA. Translation: DAA09907.1.
PIRiS53039.
RefSeqiNP_013722.1. NM_001182505.1.

Genome annotation databases

EnsemblFungiiYMR009W; YMR009W; YMR009W.
GeneIDi855021.
KEGGisce:YMR009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48613 Genomic DNA. Translation: CAA88525.1 .
AY558584 Genomic DNA. Translation: AAS56910.1 .
BK006946 Genomic DNA. Translation: DAA09907.1 .
PIRi S53039.
RefSeqi NP_013722.1. NM_001182505.1.

3D structure databases

ProteinModelPortali Q03677.
SMRi Q03677. Positions 1-176.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35178. 40 interactions.
DIPi DIP-877N.
MINTi MINT-565090.
STRINGi 4932.YMR009W.

Proteomic databases

MaxQBi Q03677.
PaxDbi Q03677.
PeptideAtlasi Q03677.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR009W ; YMR009W ; YMR009W .
GeneIDi 855021.
KEGGi sce:YMR009W.

Organism-specific databases

CYGDi YMR009w.
SGDi S000004611. ADI1.

Phylogenomic databases

eggNOGi COG1791.
GeneTreei ENSGT00390000008195.
HOGENOMi HOG000201071.
KOi K08967.
OMAi TTDESNY.
OrthoDBi EOG7M0P3P.

Enzyme and pathway databases

UniPathwayi UPA00904 ; UER00878 .
BioCyci YEAST:G3O-32717-MONOMER.
YEAST:MONOMER3O-186.
Reactomei REACT_189018. Methionine salvage pathway.

Miscellaneous databases

NextBioi 978207.
PROi Q03677.

Gene expression databases

Genevestigatori Q03677.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
HAMAPi MF_03154. Salvage_MtnD_euk.
InterProi IPR004313. Acireductn_dOase_family.
IPR027496. MTCBP-1_eukaryotes.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR23418. PTHR23418. 1 hit.
Pfami PF03079. ARD. 1 hit.
[Graphical view ]
SUPFAMi SSF51182. SSF51182. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. "Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 (MTCBP-1) acts as an eukaryotic aci-reductone dioxygenase (ARD) in the methionine salvage pathway."
    Hirano W., Gotoh I., Uekita T., Seiki M.
    Genes Cells 10:565-574(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLU-24; GLU-45; GLU-91 AND GLU-151.
  6. "Regulation and surveillance of normal and 3'-extended forms of the yeast aci-reductone dioxygenase mRNA by RNase III cleavage and exonucleolytic degradation."
    Zer C., Chanfreau G.
    J. Biol. Chem. 280:28997-29003(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "A complete inventory of all enzymes in the eukaryotic methionine salvage pathway."
    Pirkov I., Norbeck J., Gustafsson L., Albers E.
    FEBS J. 275:4111-4120(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMTND_YEAST
AccessioniPrimary (citable) accession number: Q03677
Secondary accession number(s): D6VZI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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