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Q03674 (PLB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysophospholipase 2

EC=3.1.1.5
Alternative name(s):
Phospholipase B 2
Gene names
Name:PLB2
Ordered Locus Names:YMR006C
ORF Names:YM8270.08C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol > phosphatidylcholine > phosphatidylethanolamine. Ref.5

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Subcellular location

Secretedcell wall. Membrane; Lipid-anchorGPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP). Ref.5 Ref.7

Post-translational modification

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Miscellaneous

Present with 623 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the lysophospholipase family.

Contains 1 PLA2c domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

YHR080CP388001EBI-2079187,EBI-24597

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 680661Lysophospholipase 2
PRO_0000024647
Propeptide681 – 70626Removed in mature form Potential
PRO_0000372443

Regions

Domain36 – 588553PLA2c
Compositional bias369 – 3724Poly-Asp
Compositional bias657 – 66711Poly-Ser
Compositional bias671 – 6744Poly-Ser

Amino acid modifications

Lipidation6801GPI-anchor amidated asparagine Potential
Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation801N-linked (GlcNAc...) Potential
Glycosylation941N-linked (GlcNAc...) Potential
Glycosylation1251N-linked (GlcNAc...) Potential
Glycosylation1621N-linked (GlcNAc...) Potential
Glycosylation1811N-linked (GlcNAc...) Potential
Glycosylation1931N-linked (GlcNAc...) Potential
Glycosylation2171N-linked (GlcNAc...) Potential
Glycosylation2791N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Potential
Glycosylation3651N-linked (GlcNAc...) Potential
Glycosylation3901N-linked (GlcNAc...) Potential
Glycosylation4911N-linked (GlcNAc...) Potential
Glycosylation5151N-linked (GlcNAc...) Potential
Glycosylation5241N-linked (GlcNAc...) Potential
Glycosylation5431N-linked (GlcNAc...) Potential
Glycosylation5671N-linked (GlcNAc...) Potential
Glycosylation5841N-linked (GlcNAc...) Potential
Glycosylation5981N-linked (GlcNAc...) Potential
Glycosylation6301N-linked (GlcNAc...) Potential
Glycosylation6341N-linked (GlcNAc...) Potential
Glycosylation6421N-linked (GlcNAc...) Potential
Glycosylation6481N-linked (GlcNAc...) Potential
Glycosylation6521N-linked (GlcNAc...) Potential
Glycosylation6581N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q03674 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 5E7BF22C77780DC2

FASTA70675,455
        10         20         30         40         50         60 
MQLRNILQAS SLISGLSLAA DSSSTTGDGY APSIIPCPSD DTSLVRNASG LSTAETDWLK 

        70         80         90        100        110        120 
KRDAYTKEAL HSFLSRATSN FSDTSLLSTL FSSNSSNVPK IGIACSGGGY RAMLGGAGMI 

       130        140        150        160        170        180 
AAMDNRTDGA NEHGLGGLLQ SSTYLSGLSG GNWLTGTLAW NNWTSVQEIV DHMSESDSIW 

       190        200        210        220        230        240 
NITKSIVNPG GSNLTYTIER WESIVQEVQA KSDAGFNISL SDLWARALSY NFFPSLPDAG 

       250        260        270        280        290        300 
SALTWSSLRD VDVFKNGEMP LPITVADGRY PGTTVINLNA TLFEFTPFEM GSWDPSLNAF 

       310        320        330        340        350        360 
TDVKYLGTNV TNGKPVNKDQ CVSGYDNAGF VIATSASLFN EFSLEASTST YYKMINSFAN 

       370        380        390        400        410        420 
KYVNNLSQDD DDIAIYAANP FKDTEFVDRN YTSSIVDADD LFLVDGGEDG QNLPLVPLIK 

       430        440        450        460        470        480 
KERDLDVVFA LDISDNTDES WPSGVCMTNT YERQYSKQGK GMAFPYVPDV NTFLNLGLTN 

       490        500        510        520        530        540 
KPTFFGCDAK NLTDLEYIPP LVVYIPNTKH SFNGNQSTLK MNYNVTERLG MIRNGFEAAT 

       550        560        570        580        590        600 
MGNFTDDSNF LGCIGCAIIR RKQESLNATL PPECTKCFAD YCWNGTLSTS ANPELSGNST 

       610        620        630        640        650        660 
YQSGAIASAI SEATDGIPIT ALLGSSTSGN TTSNSTTSTS SNVTSNSNSS SNTTLNSNSS 

       670        680        690        700 
SSSISSSTAR SSSSTANKAN AAAISYANTN TLMSLLGAIT ALFGLI 

« Hide

References

« Hide 'large scale' references
[1]"The PLB2 gene of Saccharomyces cerevisiae confers resistance to lysophosphatidylcholine and encodes a phospholipase B/lysophospholipase."
Fyrst H., Oskouian B., Kuypers F.A., Saba J.D.
Biochemistry 38:5864-5871(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: DG338.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Characterization and function in vivo of two novel phospholipases B/lysophospholipases from Saccharomyces cerevisiae."
Merkel O., Fido M., Mayr J.A., Prueger H., Raab F., Zandonella G., Kohlwein S.D., Paltauf F.
J. Biol. Chem. 274:28121-28127(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages."
Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G.
J. Biol. Chem. 280:20894-20901(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, PROBABLE GPI-ANCHOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF129165 Genomic DNA. Translation: AAD28616.1.
Z48613 Genomic DNA. Translation: CAA88521.1.
AY693181 Genomic DNA. Translation: AAT93200.1.
BK006946 Genomic DNA. Translation: DAA09905.1.
PIRS53035.
RefSeqNP_013719.1. NM_001182502.1.

3D structure databases

ProteinModelPortalQ03674.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35176. 53 interactions.
STRING4932.YMR006C.

Proteomic databases

PaxDbQ03674.
PeptideAtlasQ03674.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR006C; YMR006C; YMR006C.
GeneID855018.
KEGGsce:YMR006C.

Organism-specific databases

CYGDYMR006c.
SGDS000004608. PLB2.

Phylogenomic databases

eggNOGNOG257248.
GeneTreeENSGT00510000051940.
HOGENOMHOG000189547.
KOK13333.
OMAHAPNEND.
OrthoDBEOG7N37NC.

Enzyme and pathway databases

BioCycYEAST:YMR006C-MONOMER.

Gene expression databases

GenevestigatorQ03674.

Family and domain databases

InterProIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMSSF52151. SSF52151. 1 hit.
PROSITEPS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978201.

Entry information

Entry namePLB2_YEAST
AccessionPrimary (citable) accession number: Q03674
Secondary accession number(s): D6VZI1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families