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Protein

Lysophospholipase 2

Gene

PLB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol > phosphatidylcholine > phosphatidylethanolamine.1 Publication

Miscellaneous

Present with 623 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

GO - Molecular functioni

  • lysophospholipase activity Source: SGD

GO - Biological processi

  • glycerophospholipid metabolic process Source: SGD
  • phospholipid catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YMR006C-MONOMER
YEAST:YMR006C-MONOMER

Chemistry databases

SwissLipidsiSLP:000000121

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase 2 (EC:3.1.1.5)
Alternative name(s):
Phospholipase B 2
Gene namesi
Name:PLB2
Ordered Locus Names:YMR006C
ORF Names:YM8270.08C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR006C
SGDiS000004608 PLB2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002464720 – 680Lysophospholipase 2Add BLAST661
PropeptideiPRO_0000372443681 – 706Removed in mature formSequence analysisAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi80N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi94N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi125N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi162N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi181N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi193N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi217N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi279N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi309N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi365N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi390N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi491N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi515N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi524N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi543N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi567N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi584N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi598N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi630N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi634N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi642N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi648N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi652N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi658N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi680GPI-anchor amidated asparagineSequence analysis1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ03674
PaxDbiQ03674
PRIDEiQ03674

Interactioni

Protein-protein interaction databases

BioGridi35176, 113 interactors
STRINGi4932.YMR006C

Structurei

3D structure databases

ProteinModelPortaliQ03674
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 588PLA2cPROSITE-ProRule annotationAdd BLAST553

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi369 – 372Poly-Asp4
Compositional biasi657 – 667Poly-SerAdd BLAST11
Compositional biasi671 – 674Poly-Ser4

Sequence similaritiesi

Belongs to the lysophospholipase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00510000051940
HOGENOMiHOG000189547
InParanoidiQ03674
KOiK13333
OMAiTANIHNW
OrthoDBiEOG092C2IZ9

Family and domain databases

InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR002642 LysoPLipase_cat_dom
PfamiView protein in Pfam
PF01735 PLA2_B, 1 hit
SMARTiView protein in SMART
SM00022 PLAc, 1 hit
SUPFAMiSSF52151 SSF52151, 1 hit
PROSITEiView protein in PROSITE
PS51210 PLA2C, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLRNILQAS SLISGLSLAA DSSSTTGDGY APSIIPCPSD DTSLVRNASG
60 70 80 90 100
LSTAETDWLK KRDAYTKEAL HSFLSRATSN FSDTSLLSTL FSSNSSNVPK
110 120 130 140 150
IGIACSGGGY RAMLGGAGMI AAMDNRTDGA NEHGLGGLLQ SSTYLSGLSG
160 170 180 190 200
GNWLTGTLAW NNWTSVQEIV DHMSESDSIW NITKSIVNPG GSNLTYTIER
210 220 230 240 250
WESIVQEVQA KSDAGFNISL SDLWARALSY NFFPSLPDAG SALTWSSLRD
260 270 280 290 300
VDVFKNGEMP LPITVADGRY PGTTVINLNA TLFEFTPFEM GSWDPSLNAF
310 320 330 340 350
TDVKYLGTNV TNGKPVNKDQ CVSGYDNAGF VIATSASLFN EFSLEASTST
360 370 380 390 400
YYKMINSFAN KYVNNLSQDD DDIAIYAANP FKDTEFVDRN YTSSIVDADD
410 420 430 440 450
LFLVDGGEDG QNLPLVPLIK KERDLDVVFA LDISDNTDES WPSGVCMTNT
460 470 480 490 500
YERQYSKQGK GMAFPYVPDV NTFLNLGLTN KPTFFGCDAK NLTDLEYIPP
510 520 530 540 550
LVVYIPNTKH SFNGNQSTLK MNYNVTERLG MIRNGFEAAT MGNFTDDSNF
560 570 580 590 600
LGCIGCAIIR RKQESLNATL PPECTKCFAD YCWNGTLSTS ANPELSGNST
610 620 630 640 650
YQSGAIASAI SEATDGIPIT ALLGSSTSGN TTSNSTTSTS SNVTSNSNSS
660 670 680 690 700
SNTTLNSNSS SSSISSSTAR SSSSTANKAN AAAISYANTN TLMSLLGAIT

ALFGLI
Length:706
Mass (Da):75,455
Last modified:November 1, 1997 - v1
Checksum:i5E7BF22C77780DC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129165 Genomic DNA Translation: AAD28616.1
Z48613 Genomic DNA Translation: CAA88521.1
AY693181 Genomic DNA Translation: AAT93200.1
BK006946 Genomic DNA Translation: DAA09905.1
PIRiS53035
RefSeqiNP_013719.1, NM_001182502.1

Genome annotation databases

EnsemblFungiiYMR006C; YMR006C; YMR006C
GeneIDi855018
KEGGisce:YMR006C

Similar proteinsi

Entry informationi

Entry nameiPLB2_YEAST
AccessioniPrimary (citable) accession number: Q03674
Secondary accession number(s): D6VZI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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