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Q03674

- PLB2_YEAST

UniProt

Q03674 - PLB2_YEAST

Protein

Lysophospholipase 2

Gene

PLB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol > phosphatidylcholine > phosphatidylethanolamine.1 Publication

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    GO - Molecular functioni

    1. lysophospholipase activity Source: SGD

    GO - Biological processi

    1. glycerophospholipid metabolic process Source: SGD
    2. phospholipid catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:YMR006C-MONOMER.
    YEAST:YMR006C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysophospholipase 2 (EC:3.1.1.5)
    Alternative name(s):
    Phospholipase B 2
    Gene namesi
    Name:PLB2
    Ordered Locus Names:YMR006C
    ORF Names:YM8270.08C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR006c.
    SGDiS000004608. PLB2.

    Subcellular locationi

    Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
    Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: SGD
    3. fungal-type cell wall Source: SGD

    Keywords - Cellular componenti

    Cell wall, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 680661Lysophospholipase 2PRO_0000024647Add
    BLAST
    Propeptidei681 – 70626Removed in mature formSequence AnalysisPRO_0000372443Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi125 – 1251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi193 – 1931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi524 – 5241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi543 – 5431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi567 – 5671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi584 – 5841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi598 – 5981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi630 – 6301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi634 – 6341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi642 – 6421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi648 – 6481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi652 – 6521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi658 – 6581N-linked (GlcNAc...)Sequence Analysis
    Lipidationi680 – 6801GPI-anchor amidated asparagineSequence Analysis

    Post-translational modificationi

    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    MaxQBiQ03674.
    PaxDbiQ03674.
    PeptideAtlasiQ03674.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03674.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    YHR080CP388001EBI-2079187,EBI-24597

    Protein-protein interaction databases

    BioGridi35176. 54 interactions.
    STRINGi4932.YMR006C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03674.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 588553PLA2cPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi369 – 3724Poly-Asp
    Compositional biasi657 – 66711Poly-SerAdd
    BLAST
    Compositional biasi671 – 6744Poly-Ser

    Sequence similaritiesi

    Belongs to the lysophospholipase family.Curated
    Contains 1 PLA2c domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG257248.
    GeneTreeiENSGT00510000051940.
    HOGENOMiHOG000189547.
    KOiK13333.
    OMAiHAPNEND.
    OrthoDBiEOG7N37NC.

    Family and domain databases

    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view]
    PfamiPF01735. PLA2_B. 1 hit.
    [Graphical view]
    SMARTiSM00022. PLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 1 hit.
    PROSITEiPS51210. PLA2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03674-1 [UniParc]FASTAAdd to Basket

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    MQLRNILQAS SLISGLSLAA DSSSTTGDGY APSIIPCPSD DTSLVRNASG    50
    LSTAETDWLK KRDAYTKEAL HSFLSRATSN FSDTSLLSTL FSSNSSNVPK 100
    IGIACSGGGY RAMLGGAGMI AAMDNRTDGA NEHGLGGLLQ SSTYLSGLSG 150
    GNWLTGTLAW NNWTSVQEIV DHMSESDSIW NITKSIVNPG GSNLTYTIER 200
    WESIVQEVQA KSDAGFNISL SDLWARALSY NFFPSLPDAG SALTWSSLRD 250
    VDVFKNGEMP LPITVADGRY PGTTVINLNA TLFEFTPFEM GSWDPSLNAF 300
    TDVKYLGTNV TNGKPVNKDQ CVSGYDNAGF VIATSASLFN EFSLEASTST 350
    YYKMINSFAN KYVNNLSQDD DDIAIYAANP FKDTEFVDRN YTSSIVDADD 400
    LFLVDGGEDG QNLPLVPLIK KERDLDVVFA LDISDNTDES WPSGVCMTNT 450
    YERQYSKQGK GMAFPYVPDV NTFLNLGLTN KPTFFGCDAK NLTDLEYIPP 500
    LVVYIPNTKH SFNGNQSTLK MNYNVTERLG MIRNGFEAAT MGNFTDDSNF 550
    LGCIGCAIIR RKQESLNATL PPECTKCFAD YCWNGTLSTS ANPELSGNST 600
    YQSGAIASAI SEATDGIPIT ALLGSSTSGN TTSNSTTSTS SNVTSNSNSS 650
    SNTTLNSNSS SSSISSSTAR SSSSTANKAN AAAISYANTN TLMSLLGAIT 700
    ALFGLI 706
    Length:706
    Mass (Da):75,455
    Last modified:November 1, 1997 - v1
    Checksum:i5E7BF22C77780DC2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF129165 Genomic DNA. Translation: AAD28616.1.
    Z48613 Genomic DNA. Translation: CAA88521.1.
    AY693181 Genomic DNA. Translation: AAT93200.1.
    BK006946 Genomic DNA. Translation: DAA09905.1.
    PIRiS53035.
    RefSeqiNP_013719.1. NM_001182502.1.

    Genome annotation databases

    EnsemblFungiiYMR006C; YMR006C; YMR006C.
    GeneIDi855018.
    KEGGisce:YMR006C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF129165 Genomic DNA. Translation: AAD28616.1 .
    Z48613 Genomic DNA. Translation: CAA88521.1 .
    AY693181 Genomic DNA. Translation: AAT93200.1 .
    BK006946 Genomic DNA. Translation: DAA09905.1 .
    PIRi S53035.
    RefSeqi NP_013719.1. NM_001182502.1.

    3D structure databases

    ProteinModelPortali Q03674.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35176. 54 interactions.
    STRINGi 4932.YMR006C.

    Proteomic databases

    MaxQBi Q03674.
    PaxDbi Q03674.
    PeptideAtlasi Q03674.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR006C ; YMR006C ; YMR006C .
    GeneIDi 855018.
    KEGGi sce:YMR006C.

    Organism-specific databases

    CYGDi YMR006c.
    SGDi S000004608. PLB2.

    Phylogenomic databases

    eggNOGi NOG257248.
    GeneTreei ENSGT00510000051940.
    HOGENOMi HOG000189547.
    KOi K13333.
    OMAi HAPNEND.
    OrthoDBi EOG7N37NC.

    Enzyme and pathway databases

    BioCyci MetaCyc:YMR006C-MONOMER.
    YEAST:YMR006C-MONOMER.

    Miscellaneous databases

    NextBioi 978201.

    Gene expression databases

    Genevestigatori Q03674.

    Family and domain databases

    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view ]
    Pfami PF01735. PLA2_B. 1 hit.
    [Graphical view ]
    SMARTi SM00022. PLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52151. SSF52151. 1 hit.
    PROSITEi PS51210. PLA2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The PLB2 gene of Saccharomyces cerevisiae confers resistance to lysophosphatidylcholine and encodes a phospholipase B/lysophospholipase."
      Fyrst H., Oskouian B., Kuypers F.A., Saba J.D.
      Biochemistry 38:5864-5871(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: DG338.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Characterization and function in vivo of two novel phospholipases B/lysophospholipases from Saccharomyces cerevisiae."
      Merkel O., Fido M., Mayr J.A., Prueger H., Raab F., Zandonella G., Kohlwein S.D., Paltauf F.
      J. Biol. Chem. 274:28121-28127(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages."
      Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G.
      J. Biol. Chem. 280:20894-20901(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, GPI-ANCHOR.

    Entry informationi

    Entry nameiPLB2_YEAST
    AccessioniPrimary (citable) accession number: Q03674
    Secondary accession number(s): D6VZI1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 623 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3