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Q03663 (GSTX2_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutathione S-transferase

EC=2.5.1.18
Alternative name(s):
Auxin-induced protein PGNT35/PCNT111
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Tissue specificity

Root tip-specific expression.

Induction

By auxin.

Sequence similarities

Belongs to the GST superfamily. HSP26 family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Ontologies

Keywords
   Biological processAuxin signaling pathway
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processauxin-activated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionglutathione transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 223223Probable glutathione S-transferase
PRO_0000185867

Regions

Domain2 – 8180GST N-terminal
Domain86 – 212127GST C-terminal
Region65 – 662Glutathione binding By similarity

Sites

Binding site121Glutathione By similarity
Binding site391Glutathione By similarity
Binding site531Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03663 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: ACD523DB2A003A83

FASTA22325,842
        10         20         30         40         50         60 
MAEVKLLGFW YSPFSHRVEW ALKIKGVKYE YIEEDRDNKS SLLLQSNPVY KKVPVLIHNG 

        70         80         90        100        110        120 
KPIVESMIIL EYIDETFEGP SILPKDPYDR ALARFWAKFL DDKVAAVVNT FFRKGEEQEK 

       130        140        150        160        170        180 
GKEEVYEMLK VLDNELKDKK FFAGDKFGFA DIAANLVGFW LGVFEEGYGD VLVKSEKFPN 

       190        200        210        220 
FSKWRDEYIN CSQVNESLPP RDELLAFFRA RFQAVVASRS APK 

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References

[1]"Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression."
van der Zaal E.J., Droog F.N.J., Boot C.J.M., Hensgens L.A.M., Hoge J.H.C., Schilperoort R.A., Libbenga K.R.
Plant Mol. Biol. 16:983-998(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Samsun NN and cv. White Burley.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56269 Genomic DNA. Translation: CAA39710.1.
X56265 mRNA. Translation: CAA39706.1.
PIRS16268.

3D structure databases

ProteinModelPortalQ03663.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTX2_TOBAC
AccessionPrimary (citable) accession number: Q03663
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families