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Q03663

- GSTX2_TOBAC

UniProt

Q03663 - GSTX2_TOBAC

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Protein

Probable glutathione S-transferase

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121GlutathioneBy similarity
Binding sitei39 – 391GlutathioneBy similarity
Binding sitei53 – 531Glutathione; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. auxin-activated signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Auxin signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutathione S-transferase (EC:2.5.1.18)
Alternative name(s):
Auxin-induced protein PGNT35/PCNT111
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Probable glutathione S-transferasePRO_0000185867Add
BLAST

Expressioni

Tissue specificityi

Root tip-specific expression.

Inductioni

By auxin.

Structurei

3D structure databases

ProteinModelPortaliQ03663.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8180GST N-terminalAdd
BLAST
Domaini86 – 212127GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 662Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. HSP26 family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03663-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEVKLLGFW YSPFSHRVEW ALKIKGVKYE YIEEDRDNKS SLLLQSNPVY
60 70 80 90 100
KKVPVLIHNG KPIVESMIIL EYIDETFEGP SILPKDPYDR ALARFWAKFL
110 120 130 140 150
DDKVAAVVNT FFRKGEEQEK GKEEVYEMLK VLDNELKDKK FFAGDKFGFA
160 170 180 190 200
DIAANLVGFW LGVFEEGYGD VLVKSEKFPN FSKWRDEYIN CSQVNESLPP
210 220
RDELLAFFRA RFQAVVASRS APK
Length:223
Mass (Da):25,842
Last modified:October 1, 1993 - v1
Checksum:iACD523DB2A003A83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56269 Genomic DNA. Translation: CAA39710.1.
X56265 mRNA. Translation: CAA39706.1.
PIRiS16268.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56269 Genomic DNA. Translation: CAA39710.1 .
X56265 mRNA. Translation: CAA39706.1 .
PIRi S16268.

3D structure databases

ProteinModelPortali Q03663.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression."
    van der Zaal E.J., Droog F.N.J., Boot C.J.M., Hensgens L.A.M., Hoge J.H.C., Schilperoort R.A., Libbenga K.R.
    Plant Mol. Biol. 16:983-998(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Samsun NN and cv. White Burley.
    Tissue: Leaf.

Entry informationi

Entry nameiGSTX2_TOBAC
AccessioniPrimary (citable) accession number: Q03663
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 1, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3