Q03663 (GSTX2_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Probable glutathione S-transferase EC=2.5.1.18 Alternative name(s): Auxin-induced protein PGNT35/PCNT111
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Tissue specificity	Root tip-specific expression.
Induction	By auxin.
Sequence similarities	Belongs to the GST superfamily. HSP26 family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Auxin signaling pathway
Molecular function	Transferase
Gene Ontology (GO)
Biological_process	auxin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Probable glutathione S-transferase		PRO_0000185867
Regions
	Domain	2 – 81	80	GST N-terminal
	Domain	86 – 212	127	GST C-terminal
	Region	65 – 66	2	Glutathione binding By similarity
Sites
	Binding site	12	1	Glutathione By similarity
	Binding site	39	1	Glutathione By similarity
	Binding site	53	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03663 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: ACD523DB2A003A83	FASTA	223	25,842
	10 20 30 40 50 60 MAEVKLLGFW YSPFSHRVEW ALKIKGVKYE YIEEDRDNKS SLLLQSNPVY KKVPVLIHNG 70 80 90 100 110 120 KPIVESMIIL EYIDETFEGP SILPKDPYDR ALARFWAKFL DDKVAAVVNT FFRKGEEQEK 130 140 150 160 170 180 GKEEVYEMLK VLDNELKDKK FFAGDKFGFA DIAANLVGFW LGVFEEGYGD VLVKSEKFPN 190 200 210 220 FSKWRDEYIN CSQVNESLPP RDELLAFFRA RFQAVVASRS APK

References

[1]

"Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression." van der Zaal E.J., Droog F.N.J., Boot C.J.M., Hensgens L.A.M., Hoge J.H.C., Schilperoort R.A., Libbenga K.R. Plant Mol. Biol. 16:983-998(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Samsun NN and cv. White Burley. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56269 Genomic DNA. Translation: CAA39710.1. X56265 mRNA. Translation: CAA39706.1.
PIR	S16268.
3D structure databases
ProteinModelPortal	Q03663.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTX2_TOBAC

Accession

Primary (citable) accession number: Q03663

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program