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Protein

Probable glutathione S-transferase

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121GlutathioneBy similarity
Binding sitei39 – 391GlutathioneBy similarity
Binding sitei53 – 531Glutathione; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Auxin signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutathione S-transferase (EC:2.5.1.18)
Alternative name(s):
Auxin-induced protein PGNT1/PCNT110
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Probable glutathione S-transferasePRO_0000185866Add
BLAST

Expressioni

Tissue specificityi

Root tip-specific expression.

Inductioni

By auxin.

Structurei

3D structure databases

ProteinModelPortaliQ03662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8180GST N-terminalAdd
BLAST
Domaini86 – 218133GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 662Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. HSP26 family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEVKLLGFW YSPFSRRVEW ALKIKGVKYE YIEEDRDNKS SLLLQSNPIH
60 70 80 90 100
KKVPVLIHNG KRIVESMVIL EYIDETFEGP SILPKDPYDR ALARFWAKFL
110 120 130 140 150
DDKVPAVVKT FLRKGEEQEK DKEEVCEMLK VLDNELKDKK FFVGDKFGFA
160 170 180 190 200
DIAANLVAFW LGVFEEASGV VLVTSEKFPN FCKWRGEYIN CSQIKESLPP
210 220
RDELLAFYRS RCQAAAASAS APK
Length:223
Mass (Da):25,668
Last modified:October 1, 1993 - v1
Checksum:i0B29A74FC15869BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56268 Genomic DNA. Translation: CAA39709.1.
X56264 mRNA. Translation: CAA39705.1.
PIRiS16267.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56268 Genomic DNA. Translation: CAA39709.1.
X56264 mRNA. Translation: CAA39705.1.
PIRiS16267.

3D structure databases

ProteinModelPortaliQ03662.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression."
    van der Zaal E.J., Droog F.N.J., Boot C.J.M., Hensgens L.A.M., Hoge J.H.C., Schilperoort R.A., Libbenga K.R.
    Plant Mol. Biol. 16:983-998(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Samsun NN and cv. White Burley.
    Tissue: Leaf.

Entry informationi

Entry nameiGSTX1_TOBAC
AccessioniPrimary (citable) accession number: Q03662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 27, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.